##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043225_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1385358
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19362937233553	33.0	31.0	34.0	30.0	34.0
2	32.49659582577211	34.0	31.0	34.0	31.0	34.0
3	32.566437700579925	34.0	31.0	34.0	31.0	34.0
4	36.135398936592566	37.0	35.0	37.0	35.0	37.0
5	36.09534358627878	37.0	35.0	37.0	35.0	37.0
6	36.32988223982537	37.0	37.0	37.0	35.0	37.0
7	36.27223793416575	37.0	37.0	37.0	35.0	37.0
8	36.16607187456239	37.0	37.0	37.0	35.0	37.0
9	37.679836547664934	39.0	38.0	39.0	35.0	39.0
10	37.472517573074974	39.0	37.0	39.0	35.0	39.0
11	37.28906607533937	39.0	37.0	39.0	34.0	39.0
12	37.3364559918808	39.0	37.0	39.0	34.0	39.0
13	37.34231223986868	39.0	37.0	39.0	34.0	39.0
14	38.75659143701483	40.0	38.0	41.0	35.0	41.0
15	38.858816277092274	40.0	38.0	41.0	35.0	41.0
16	38.903768556575265	41.0	38.0	41.0	35.0	41.0
17	38.848271710272726	41.0	38.0	41.0	35.0	41.0
18	38.613775644995734	40.0	38.0	41.0	35.0	41.0
19	38.35467727475497	40.0	37.0	41.0	35.0	41.0
20	38.081122713406934	40.0	35.0	41.0	34.0	41.0
21	38.01124907785569	40.0	35.0	41.0	34.0	41.0
22	37.97242950919546	40.0	35.0	41.0	34.0	41.0
23	37.928982255850116	40.0	35.0	41.0	34.0	41.0
24	37.854349561629554	40.0	35.0	41.0	34.0	41.0
25	37.830279970953356	40.0	35.0	41.0	34.0	41.0
26	37.80691705681853	40.0	35.0	41.0	34.0	41.0
27	37.80327395517982	40.0	35.0	41.0	34.0	41.0
28	37.73392076271982	40.0	35.0	41.0	34.0	41.0
29	37.747128179142145	40.0	35.0	41.0	34.0	41.0
30	37.67142355983074	40.0	35.0	41.0	34.0	41.0
31	37.52775022773897	40.0	35.0	41.0	33.0	41.0
32	37.28545256893886	40.0	35.0	41.0	33.0	41.0
33	36.999475947733366	40.0	35.0	41.0	33.0	41.0
34	36.693626484995214	40.0	35.0	41.0	31.0	41.0
35	36.477095451139704	40.0	35.0	41.0	31.0	41.0
36	36.41327729005788	40.0	35.0	41.0	30.0	41.0
37	36.26466516236236	39.0	35.0	41.0	30.0	41.0
38	36.17174910745093	39.0	35.0	41.0	30.0	41.0
39	36.08640943351827	39.0	35.0	41.0	30.0	41.0
40	36.03044555992025	39.0	35.0	41.0	30.0	41.0
41	35.96008468569135	39.0	35.0	41.0	29.0	41.0
42	35.89108952343005	39.0	35.0	41.0	29.0	41.0
43	35.79413696676238	39.0	35.0	41.0	28.0	41.0
44	35.71452361050357	39.0	35.0	41.0	27.0	41.0
45	35.657766440154816	39.0	35.0	41.0	27.0	41.0
46	35.56716314483332	39.0	35.0	41.0	27.0	41.0
47	35.42573688533938	38.0	35.0	40.0	26.0	41.0
48	35.39569988407329	38.0	35.0	40.0	26.0	41.0
49	35.33628275146208	38.0	35.0	40.0	26.0	41.0
50	35.243548598990294	38.0	35.0	40.0	25.0	41.0
51	34.74471003163082	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	5.0
15	14.0
16	46.0
17	92.0
18	205.0
19	420.0
20	821.0
21	1421.0
22	2301.0
23	3731.0
24	6840.0
25	13199.0
26	21022.0
27	24166.0
28	22723.0
29	21300.0
30	22105.0
31	25178.0
32	30671.0
33	39609.0
34	70773.0
35	113686.0
36	121875.0
37	109985.0
38	194027.0
39	538981.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.252666819695705	19.391088801594965	26.019844689964618	27.336399688744716
2	33.91152323081831	20.006958490152005	25.989744167211654	20.09177411181803
3	21.226715404971134	22.188271912386544	37.08124542537019	19.503767257272127
4	22.569761751114154	24.30606384775632	34.68468078287345	18.439493618256076
5	20.334815982583564	29.769777920219898	30.95582513689602	18.939580960300514
6	74.60995641559799	1.660004128896646	20.154573763604784	3.575465691900577
7	75.39971617444733	1.3553175424691668	16.991275901247185	6.2536903818363205
8	64.31471143199087	3.384323763243869	24.110807459154962	8.190157345610306
9	33.96753763287179	20.72135866685723	28.118941096813966	17.19216260345701
10	24.62959032971983	20.57063950256901	42.02184561680086	12.777924550910305
11	22.846802054053896	17.137591871559554	40.353829118538314	19.661776955848236
12	19.357667837483163	19.42205552644154	42.288202760586074	18.932073875489223
13	14.61838744930913	22.473974236262396	41.61552465138975	21.292113663038723
14	13.504956841480686	22.598057686172094	42.478045386102366	21.418940086244856
15	14.620119853496352	19.394264875938205	44.910124314437134	21.075490956128306
16	15.276989774484285	24.597901769795243	38.5471481017903	21.577960353930177
17	16.35302932527188	21.769174466094686	40.63534479896171	21.242451409671723
18	18.060241468270295	27.104113160641507	38.16017231646982	16.675473054618372
19	17.39629756351788	23.40903939631489	36.47952370434213	22.715139335825107
20	15.281826069506943	28.467082154937568	35.42983113390185	20.821260641653637
21	15.420418404484618	23.273767502696057	37.04970123246121	24.25611286035812
22	13.555268746417893	25.56119068139788	34.535838389788054	26.347702182396176
23	13.471535877368881	22.95616006837222	42.093162922508114	21.479141131750783
24	17.278927179833662	21.987818311223524	38.44493625474426	22.288318254198554
25	14.215170374733463	25.515931622006732	38.192077426917805	22.076820576341998
26	15.416087394016564	22.642306176454028	37.6119385747222	24.329667854807205
27	19.991005934928012	22.77064845332398	38.413031144296276	18.825314467451733
28	16.200649940304242	22.388003678471556	35.955760171739	25.455586209485205
29	18.041040655195264	25.615689229787534	34.01041463650551	22.332855478511693
30	19.59421319254662	21.905023827775924	36.023179568025014	22.47758341165244
31	22.357181320640585	23.35042638798058	32.81238495753444	21.48000733384439
32	17.124526656647596	23.849430977408005	34.91732822851566	24.108714137428734
33	18.30162311835641	21.626684221695765	39.865363321249816	20.206329338698012
34	23.0621254578239	19.520874748620933	35.34017921721317	22.076820576341998
35	20.07040779350897	20.08195715475711	38.671592469238995	21.176042582494922
36	23.661320756078936	20.495207736917102	35.35057364233649	20.492897864667473
37	21.31008735648114	19.674986537775794	40.53580374170431	18.47912236403875
38	22.055454258032942	20.15154205627715	35.60855749921681	22.1844461864731
39	25.581257696566517	19.3440251545088	36.300436421488165	18.77428072743652
40	19.69960111393589	18.833759937864436	40.07289090617732	21.393748042022352
41	21.958006522501766	22.057691946774767	33.28439291504434	22.699908615679124
42	21.215526961262	18.3253714924229	37.3468807340774	23.1122208122377
43	23.594911928902132	20.603916099665213	34.569475904423264	21.231696067009395
44	17.98517062015739	22.717449208074736	35.7647626100979	23.53261756166998
45	20.31207817762629	20.78762312701843	36.245071671004894	22.655227024350385
46	24.138020641595894	18.80993938029015	35.048196928158646	22.003843049955318
47	19.123143620638132	17.739746693634427	39.05986755769988	24.077242128027557
48	20.530361105216123	16.483248373344654	33.90914117506089	29.077249346378338
49	21.327627948876753	16.49725197385802	38.44645210840808	23.72866796885715
50	20.225024867218437	15.675587104560698	39.162945606839536	24.936442421381333
51	18.45457997138646	15.998319567938395	37.67661499771178	27.870485462963362
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	1512.5
2	2747.0
3	41831.0
4	80915.0
5	46402.5
6	11890.0
7	11110.5
8	10331.0
9	10552.5
10	10774.0
11	10843.0
12	10912.0
13	10484.0
14	10056.0
15	9465.0
16	8874.0
17	8142.5
18	7411.0
19	6646.0
20	5881.0
21	5594.0
22	5307.0
23	5377.0
24	5447.0
25	5394.5
26	5930.5
27	6519.0
28	7531.5
29	8544.0
30	9720.0
31	10896.0
32	12566.5
33	14237.0
34	17573.0
35	20909.0
36	23742.5
37	26576.0
38	30512.5
39	34449.0
40	40023.0
41	45597.0
42	55416.5
43	65236.0
44	78131.0
45	91026.0
46	120815.0
47	150604.0
48	180438.0
49	210272.0
50	206721.5
51	203171.0
52	162810.0
53	122449.0
54	96736.0
55	71023.0
56	56431.5
57	41840.0
58	35411.5
59	28983.0
60	22801.0
61	16619.0
62	13808.5
63	10998.0
64	9633.0
65	8268.0
66	7008.0
67	5748.0
68	4652.0
69	3556.0
70	3282.0
71	3008.0
72	3140.5
73	3273.0
74	2798.0
75	1965.5
76	1608.0
77	1204.0
78	800.0
79	603.5
80	407.0
81	273.0
82	139.0
83	107.0
84	75.0
85	47.5
86	20.0
87	13.0
88	6.0
89	7.5
90	9.0
91	5.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1385358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.88259309165874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36161589633244	23.794375709740045
2	12.295905094628235	8.086424878406278
3	4.989750465906508	4.92227802597955
4	2.508249870659644	3.2991103947642695
5	1.434888379626357	2.3591425359601526
6	0.9347503115620803	1.8442208482438271
7	0.6793700572797294	1.5637614406530367
8	0.5194576907216941	1.3664892697867348
9	0.36446634418701945	1.0786138642355585
>10	3.4130943993658507	23.75230890218602
>50	0.3259689587629097	7.204365820578963
>100	0.15875478173527502	9.573769173187548
>500	0.00819236647732978	1.7918554272293132
>1k	0.004649721514160145	2.78610912331007
>5k	6.642459305943065E-4	1.536448136232587
>10k+	2.2141531019810216E-4	5.040726449506085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69234	4.99755297908555	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	8128	0.5867075514054851	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	7890	0.5695278765488776	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	5085	0.36705313716743254	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	4706	0.3396955877109022	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCC	2953	0.2131578985359741	No Hit
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2937	0.21200296241116018	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2547	0.1838513943688202	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	2105	0.1519462839208349	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	1979	0.14285116193792508	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	1826	0.13180708524439172	No Hit
GCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1795	0.12956939650256466	No Hit
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1670	0.12054645802745571	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	1668	0.12040209101185397	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC	1618	0.11679291562181038	No Hit
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	1583	0.11426649284877989	No Hit
TGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT	1515	0.1093580143183206	TruSeq Adapter, Index 14 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	1488	0.10740905960769707	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5844698626636581	0.0
2	0.0	0.0	0.0	1.8923628405076522	0.0
3	0.0	0.0	0.0	3.2518670264292697	0.0
4	0.0	0.0	0.0	4.1156148807745	0.0
5	0.0	0.0	0.0	8.369244628464267	0.0
6	0.0	0.0	0.0	9.552260137812754	0.0
7	0.0	0.0	0.0	13.177749000619334	0.0
8	0.0	0.0	0.0	17.48825935245619	0.0
9	0.0	0.0	0.0	20.964472721130566	0.0
10	0.0	0.0	0.0	23.1120764452221	0.0
11	0.0	0.0	0.0	25.051936033862727	0.0
12	0.0	0.0	0.0	26.456843646191093	0.0
13	0.0	0.0	0.0	27.090614844682747	0.0
14	0.0	0.0	0.0	27.534254683626905	0.0
15	0.0	0.0	0.0	28.91194911351434	0.0
16	0.0	0.0	0.0	30.569571186653558	0.0
17	0.0	0.0	0.0	32.34586294661741	0.0
18	0.0	0.0	0.0	33.38508890842656	0.0
19	0.0	0.0	0.0	34.45217770424684	0.0
20	7.218350780087169E-5	0.0	0.0	35.70095238920192	0.0
21	2.1655052340261506E-4	0.0	0.0	36.948933055571196	0.0
22	3.6091753900435844E-4	0.0	0.0	38.16356494133646	0.0
23	4.3310104680523013E-4	0.0	0.0	39.108447058449876	0.0
24	8.662020936104603E-4	0.0	0.0	39.9308337628252	0.0
25	8.662020936104603E-4	0.0	0.0	40.70550716854416	0.0
26	8.662020936104603E-4	0.0	0.0	41.4957722119481	0.0
27	9.38385601411332E-4	0.0	0.0	42.26409346898058	0.0
28	9.38385601411332E-4	0.0	0.0	43.040210544855555	0.0
29	9.38385601411332E-4	0.0	0.0	43.917167981128344	0.0
30	9.38385601411332E-4	0.0	0.0	44.673506775865874	0.0
31	9.38385601411332E-4	0.0	0.0	45.35701241123233	0.0
32	9.38385601411332E-4	0.0	0.0	46.057048069885184	0.0
33	9.38385601411332E-4	0.0	0.0	46.75434075524161	0.0
34	9.38385601411332E-4	0.0	0.0	47.49205620496652	0.0
35	9.38385601411332E-4	0.0	0.0	48.136582746120496	0.0
36	9.38385601411332E-4	0.0	0.0	48.77995435114967	0.0
37	9.38385601411332E-4	0.0	0.0	49.43321509674755	0.0
38	9.38385601411332E-4	0.0	0.0	50.09304454155532	0.0
39	9.38385601411332E-4	0.0	0.0	50.72609390496897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGC	20	7.033788E-4	45.000004	9
AGCGTAG	20	7.033788E-4	45.000004	35
CCGCTTA	20	7.033788E-4	45.000004	34
GCCGATT	30	2.1658252E-6	45.000004	8
CCCGTAG	20	7.033788E-4	45.000004	23
ACGTGTC	20	7.033788E-4	45.000004	9
TCGTCGT	20	7.033788E-4	45.000004	44
ACGTCCG	20	7.033788E-4	45.000004	39
ATAACGG	115	0.0	45.000004	1
CCACGTC	20	7.033788E-4	45.000004	37
GCGTCAT	40	6.8175723E-9	45.000004	36
GCCCGTA	20	7.033788E-4	45.000004	22
GTCGGCG	20	7.033788E-4	45.000004	3
ACGTTGG	70	0.0	45.0	1
GTCCTCG	25	3.891223E-5	45.0	22
GGCGTAG	25	3.891223E-5	45.0	7
CCTACGG	35	1.2121745E-7	45.0	1
GTGATAC	35	1.2121745E-7	45.0	8
ACCACGG	25	3.891223E-5	45.0	1
CATCCGG	45	3.8562575E-10	45.0	1
>>END_MODULE