FastQCFastQC Report
Thu 26 May 2016
SRR1043224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043224_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1173180
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT909357.751154980480404No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT70000.5966688828653745No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCTT65390.5573739750080977Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT47290.4030924495814794No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC46780.398745290577746No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT34510.2941577592526296No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC29140.2483847320956716No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG25820.22008557936548526No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT24800.2113912613580184No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT22960.1957073935798428No Hit
CGTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG19190.16357251231695052No Hit
ACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC18690.159310591725055No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT16760.14285957824033824No Hit
GCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC16530.14089909476806628No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC13520.11524233280485517No Hit
ACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT13310.11345232615625905No Hit
TCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC12840.10944612079987727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGG551.8189894E-1246.0000041
ATACCGG453.092282E-1046.0000041
CCGCACT700.046.00000433
CTAGCGG1450.046.0000041
ACGCCTA253.4171993E-546.024
TTCGTGG301.8614373E-646.01
CGTCTAG206.3115085E-446.020
CACGACC405.6115823E-946.026
GCCGATT253.4171993E-546.08
GCGATCG405.6115823E-946.08
GCGATAG1150.046.08
CCAACGC206.3115085E-446.039
CATCCCG206.3115085E-446.019
AACAGCG206.3115085E-446.044
TAGTCGG301.8614373E-646.01
CGTTTTT180400.045.8087581
ATTGCGG2000.044.851
TTCACGG1650.044.606061
TACGGGA2950.044.440683
TCACGAC1200.044.08333224
Produced by FastQC (version 0.11.5)