##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043224_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1173180
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26825636304744	33.0	31.0	34.0	31.0	34.0
2	32.608465026679625	34.0	31.0	34.0	31.0	34.0
3	32.65900543821068	34.0	31.0	34.0	31.0	34.0
4	36.18875620109446	37.0	35.0	37.0	35.0	37.0
5	36.100719412195915	37.0	35.0	37.0	35.0	37.0
6	36.3299041920251	37.0	37.0	37.0	35.0	37.0
7	36.26722838780068	37.0	37.0	37.0	35.0	37.0
8	36.23837944731414	37.0	37.0	37.0	35.0	37.0
9	37.77804514226291	39.0	38.0	39.0	35.0	39.0
10	37.590268330520466	39.0	37.0	39.0	35.0	39.0
11	37.38507049216659	39.0	37.0	39.0	35.0	39.0
12	37.41276615694096	39.0	37.0	39.0	35.0	39.0
13	37.4200352887025	39.0	37.0	39.0	35.0	39.0
14	38.8541417344312	41.0	38.0	41.0	35.0	41.0
15	38.91685504355683	41.0	38.0	41.0	35.0	41.0
16	38.91958352511976	41.0	38.0	41.0	35.0	41.0
17	38.89989856628991	41.0	38.0	41.0	35.0	41.0
18	38.58063638998278	40.0	38.0	41.0	35.0	41.0
19	38.25370275661024	40.0	37.0	41.0	35.0	41.0
20	37.90105184200208	40.0	35.0	41.0	34.0	41.0
21	37.81161884791763	40.0	35.0	41.0	34.0	41.0
22	37.76267409945618	40.0	35.0	41.0	34.0	41.0
23	37.75573824988493	39.0	35.0	41.0	34.0	41.0
24	37.64570654119572	39.0	35.0	41.0	34.0	41.0
25	37.56336367820795	39.0	35.0	41.0	34.0	41.0
26	37.49946896469425	39.0	35.0	41.0	34.0	41.0
27	37.51904396597283	39.0	35.0	41.0	34.0	41.0
28	37.49480983310319	39.0	35.0	41.0	34.0	41.0
29	37.43177943708553	39.0	35.0	41.0	33.0	41.0
30	37.3840646789069	39.0	35.0	41.0	33.0	41.0
31	37.18923779812135	39.0	35.0	41.0	33.0	41.0
32	36.95478017013587	39.0	35.0	41.0	33.0	41.0
33	36.676665132375255	39.0	35.0	41.0	31.0	41.0
34	36.33780920233894	39.0	35.0	41.0	30.0	41.0
35	36.2049668422578	39.0	35.0	41.0	30.0	41.0
36	36.098956681839105	39.0	35.0	41.0	30.0	41.0
37	36.03342965273871	39.0	35.0	41.0	30.0	41.0
38	35.95291174414838	39.0	35.0	41.0	29.0	41.0
39	35.94852026117049	39.0	35.0	41.0	29.0	41.0
40	35.918514635435315	39.0	35.0	41.0	29.0	41.0
41	35.892213471078605	39.0	35.0	41.0	29.0	41.0
42	35.8186765884178	39.0	35.0	41.0	28.0	41.0
43	35.72612131130773	39.0	35.0	41.0	28.0	41.0
44	35.55992345590617	39.0	35.0	41.0	27.0	41.0
45	35.47828892412077	39.0	35.0	41.0	26.0	41.0
46	35.440667246287866	38.0	35.0	41.0	26.0	41.0
47	35.367339197736065	38.0	35.0	41.0	26.0	41.0
48	35.2995363030396	38.0	35.0	41.0	25.0	41.0
49	35.255855026509145	38.0	35.0	40.0	26.0	41.0
50	35.11557391022691	38.0	35.0	40.0	24.0	41.0
51	35.031726589270185	38.0	35.0	40.0	24.0	41.0
52	34.691944970081316	37.0	35.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	1.0
14	3.0
15	27.0
16	31.0
17	98.0
18	209.0
19	461.0
20	814.0
21	1450.0
22	2330.0
23	3885.0
24	7300.0
25	13660.0
26	19502.0
27	20707.0
28	18967.0
29	17896.0
30	19067.0
31	21745.0
32	26107.0
33	33043.0
34	58742.0
35	98651.0
36	127860.0
37	87634.0
38	147456.0
39	444842.0
40	689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.22189263369645	18.974240951942583	23.772737346357765	31.031129068003203
2	36.54290049267802	18.859595288020596	24.51175437699245	20.08574984230894
3	20.82127209805827	20.48671133159447	40.048671133159445	18.643345437187815
4	21.140404711979407	23.492729163470226	37.58928723640021	17.777578888150156
5	19.7814487120476	28.70957568318587	33.60592577439097	17.90304983037556
6	71.25070321689766	1.588076850952113	23.45215567943538	3.7090642527148434
7	71.82887536439421	1.280110468981742	20.67687822840485	6.214135938219199
8	61.230075521232884	3.4905982031742786	27.375253584275217	7.904072691317615
9	31.13929661262551	22.069503401012632	30.85153173426073	15.939668252101127
10	22.638469800030688	18.947731805860993	45.57476261102303	12.83903578308529
11	20.775243355665797	16.940367207078197	43.09210862783205	19.19228080942396
12	17.466373446529946	19.380146269114714	45.27174005693926	17.88174022741608
13	13.310063246901585	22.26290935747285	44.47484614466663	19.95218125095893
14	12.685521403365213	21.958522988799672	45.4103377145877	19.945617893247412
15	13.904942123118364	19.65367633270257	47.047597129170285	19.39378441500878
16	14.051637429891406	24.289452598919176	41.23024599805657	20.428663973132853
17	14.854583269404525	22.17988714434273	42.64963603198145	20.315893554271298
18	15.997971325798257	27.161560885797577	40.793313898975434	16.04715388942873
19	16.185495831841664	23.20820334475528	38.966654733289005	21.63964609011405
20	14.161339265926799	28.413627917284646	37.55561806372424	19.869414753064323
21	14.51405581411207	23.40280263898123	39.40341635554646	22.679725191360234
22	12.90023696278491	25.200906936701955	36.841149695698874	25.05770640481427
23	12.727458701989464	22.53592799058968	44.43998363422493	20.29662967319593
24	16.57281917523313	21.910192809287576	40.44716070850168	21.06982730697762
25	13.928297447961949	24.779573466987163	39.996846178762	21.29528290628889
26	14.541246867488363	22.682708535774562	38.92974650096319	23.84629809577388
27	18.365042022537036	22.82607954448593	38.890281116282246	19.918597316694793
28	14.237883359757241	21.903799928399735	36.575376327588266	27.28294038425476
29	16.113469373838626	25.039039192621765	35.10330895514755	23.744182478392062
30	18.919688368366323	21.69556248827972	32.89222455207215	26.492524591281814
31	17.373037385567432	23.594759542440205	33.40033072503793	25.63187234695443
32	14.315876506588928	25.414173443120408	32.825141921955705	27.44480812833495
33	16.19359348096626	23.589900952965444	33.269575001278575	26.946930564789717
34	13.751086789750932	21.844559232172386	36.22462026287526	28.179733715201415
35	17.4910073475511	25.496172795308482	32.006682691488095	25.00613716565233
36	17.37235547827273	26.044170545014406	34.00228438943726	22.58118958727561
37	16.487666001807057	25.175676366797934	38.29173698835643	20.04492064303858
38	14.795001619529824	28.576007091835866	34.20131608107877	22.427675207555534
39	19.324229870949043	26.954942975502483	35.10987231285907	18.61095484068941
40	14.985765185223068	23.954550878808025	39.21717042568063	21.842513510288274
41	16.94565198861215	25.775925262960502	32.99894304369321	24.279479704734143
42	16.962614432567893	21.622598407746466	36.817879609267116	24.59690755041852
43	20.431050648664314	22.81772618012581	34.55982884126903	22.191394329940845
44	16.03573194224245	23.802741267324706	36.12634037402615	24.035186416406688
45	18.230109616597623	22.480096830835848	35.457559794745904	23.832233757820624
46	19.941867403126544	21.812339112497657	34.90453297874154	23.34126050563426
47	15.788881501559862	20.792035322797865	38.02954363354302	25.389539542099254
48	17.105559249220068	19.131505821783527	34.44177364087352	29.321161288122884
49	18.518215448609762	18.59944765509129	38.83862663870847	24.04371025759048
50	17.08459059990794	18.242895378373312	39.587360848292676	25.08515317342607
51	15.379566647914217	18.23812202731039	38.25440256397143	28.127908760803972
52	14.177705040999678	18.375696824016774	44.1874222199492	23.25917591503435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	411.0
1	1741.0
2	3071.0
3	52628.5
4	102186.0
5	56272.5
6	10359.0
7	9718.5
8	9078.0
9	9446.0
10	9814.0
11	10148.5
12	10483.0
13	10172.0
14	9474.5
15	9088.0
16	8379.5
17	7671.0
18	6939.5
19	6208.0
20	5883.0
21	5558.0
22	5431.0
23	5304.0
24	5194.5
25	5085.0
26	6071.5
27	7058.0
28	7555.0
29	8052.0
30	8894.5
31	9737.0
32	12041.0
33	14345.0
34	16373.0
35	18401.0
36	20894.0
37	23387.0
38	25218.0
39	29180.5
40	31312.0
41	36943.0
42	42574.0
43	54234.5
44	65895.0
45	83959.5
46	102024.0
47	126627.5
48	151231.0
49	154359.5
50	157488.0
51	136859.0
52	116230.0
53	94407.0
54	72584.0
55	57976.5
56	43369.0
57	35766.5
58	28164.0
59	23447.0
60	18730.0
61	16240.0
62	13750.0
63	11373.0
64	7346.0
65	5696.0
66	4711.0
67	3726.0
68	3081.5
69	2437.0
70	2212.5
71	1988.0
72	2071.0
73	2154.0
74	1709.5
75	1265.0
76	1028.5
77	792.0
78	559.5
79	327.0
80	232.0
81	137.0
82	95.5
83	54.0
84	36.0
85	18.0
86	15.5
87	13.0
88	15.5
89	9.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1173180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.66379556130408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39418718326984	26.481124865928614
2	10.972960078581059	7.6072888973257
3	3.789514994843622	3.9407691917326675
4	1.8983412785146234	2.63214855936062
5	1.1109650684357621	1.9255133004003726
6	0.7700126540158378	1.6014936731053304
7	0.5282102894488405	1.2816841440782303
8	0.4259858493329059	1.1813029114627465
9	0.3461556270295828	1.0799161098973633
>10	3.362733461775154	24.511545638805853
>50	0.26014003812096803	6.073801808489854
>100	0.1289579479553331	7.987932299244043
>500	0.007150631908654294	1.658835507271065
>1k	0.003945176225464439	3.1070686500575926
>5k	4.931470281830548E-4	1.1572018720601271
>10k+	2.465735140915274E-4	7.772372570779797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90935	7.751154980480404	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	7000	0.5966688828653745	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCTT	6539	0.5573739750080977	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	4729	0.4030924495814794	No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	4678	0.398745290577746	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	3451	0.2941577592526296	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	2914	0.2483847320956716	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	2582	0.22008557936548526	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	2480	0.2113912613580184	No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	2296	0.1957073935798428	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1919	0.16357251231695052	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1869	0.159310591725055	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	1676	0.14285957824033824	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1653	0.14089909476806628	No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1352	0.11524233280485517	No Hit
ACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	1331	0.11345232615625905	No Hit
TCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1284	0.10944612079987727	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5730578427862733	0.0
2	0.0	0.0	0.0	1.9179495047648272	0.0
3	0.0	0.0	0.0	3.301965597776982	0.0
4	0.0	0.0	0.0	4.162703080516204	0.0
5	0.0	0.0	0.0	8.282701716701615	0.0
6	0.0	0.0	0.0	9.664416372594145	0.0
7	0.0	0.0	0.0	13.263011643567056	0.0
8	0.0	0.0	0.0	17.418554697488876	0.0
9	0.0	0.0	0.0	20.578513101143898	0.0
10	0.0	0.0	0.0	22.43918239315365	0.0
11	8.523841183791063E-5	0.0	0.0	24.174380742938	0.0
12	8.523841183791063E-5	0.0	0.0	25.41332105900203	0.0
13	8.523841183791063E-5	0.0	0.0	25.969927888303584	0.0
14	8.523841183791063E-5	0.0	0.0	26.369610801411547	0.0
15	8.523841183791063E-5	0.0	0.0	27.55348710342829	0.0
16	8.523841183791063E-5	0.0	0.0	28.940316064031094	0.0
17	8.523841183791063E-5	0.0	0.0	30.4209072776556	0.0
18	8.523841183791063E-5	0.0	0.0	31.277127124567414	0.0
19	8.523841183791063E-5	0.0	0.0	32.159174210266116	0.0
20	8.523841183791063E-5	0.0	0.0	33.14205833716906	0.0
21	3.409536473516425E-4	0.0	0.0	34.15017303397603	0.0
22	5.966688828653744E-4	0.0	0.0	35.15666820095808	0.0
23	6.81907294703285E-4	0.0	0.0	35.94171397398524	0.0
24	0.0014490530012444807	0.0	0.0	36.603590241906616	0.0
25	0.0014490530012444807	0.0	0.0	37.25165788711025	0.0
26	0.0014490530012444807	0.0	0.0	37.901174585315125	0.0
27	0.0014490530012444807	0.0	0.0	38.49988919006461	0.0
28	0.0014490530012444807	0.0	0.0	39.1235786494826	0.0
29	0.0014490530012444807	0.0	0.0	39.85773709064253	0.0
30	0.0014490530012444807	0.0	0.0	40.48833086141939	0.0
31	0.0014490530012444807	0.0	0.0	41.04800627354711	0.0
32	0.0014490530012444807	0.0	0.0	41.62430317598322	0.0
33	0.0014490530012444807	0.0	0.0	42.201452462537716	0.0
34	0.0014490530012444807	0.0	0.0	42.825482875603065	0.0
35	0.0014490530012444807	0.0	0.0	43.351574353466646	0.0
36	0.0014490530012444807	0.0	0.0	43.90264068599874	0.0
37	0.0014490530012444807	0.0	0.0	44.441688402461686	0.0
38	0.0015342914130823916	0.0	0.0	44.96607511208851	0.0
39	0.0015342914130823916	0.0	0.0	45.51586286844304	0.0
40	0.0015342914130823916	0.0	0.0	46.03828909459759	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGG	55	1.8189894E-12	46.000004	1
ATACCGG	45	3.092282E-10	46.000004	1
CCGCACT	70	0.0	46.000004	33
CTAGCGG	145	0.0	46.000004	1
ACGCCTA	25	3.4171993E-5	46.0	24
TTCGTGG	30	1.8614373E-6	46.0	1
CGTCTAG	20	6.3115085E-4	46.0	20
CACGACC	40	5.6115823E-9	46.0	26
GCCGATT	25	3.4171993E-5	46.0	8
GCGATCG	40	5.6115823E-9	46.0	8
GCGATAG	115	0.0	46.0	8
CCAACGC	20	6.3115085E-4	46.0	39
CATCCCG	20	6.3115085E-4	46.0	19
AACAGCG	20	6.3115085E-4	46.0	44
TAGTCGG	30	1.8614373E-6	46.0	1
CGTTTTT	18040	0.0	45.808758	1
ATTGCGG	200	0.0	44.85	1
TTCACGG	165	0.0	44.60606	1
TACGGGA	295	0.0	44.44068	3
TCACGAC	120	0.0	44.083332	24
>>END_MODULE