FastQCFastQC Report
Thu 26 May 2016
SRR1043223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043223_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1138526
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT837457.355563245810812No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC67880.5962094848953823No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT63670.5592318488993664No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC46640.4096524804879291No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG44500.390856247463826No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC31770.2790450108297922No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT28210.2477765110326861No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC24530.2154540168603967No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC23430.2057924017545493No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT22920.20131292566002004No Hit
CGTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT18990.16679460987276531No Hit
ACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG18060.15862615346509434No Hit
GCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG16690.1465930510150844No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTC15640.13737060023223008No Hit
AGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG13830.12147285173988122No Hit
ACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC12730.11181123663403383No Hit
TCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG11990.10531160465373648No Hit
TGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCTT11510.1010956271530031Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCA207.0332567E-445.00000411
TCGATAT207.0332567E-445.00000424
GCGCAAA207.0332567E-445.00000413
GCCGATA302.1654832E-645.0000048
CCGTACG207.0332567E-445.00000413
CGCTCGA207.0332567E-445.00000427
ACAGCGC207.0332567E-445.00000410
GCGATTG1200.045.0000048
CGGTCTA950.045.00000430
CACCGTC207.0332567E-445.00000410
CTCTCGA207.0332567E-445.00000419
TAGTCCG207.0332567E-445.00000443
CCGCACT800.045.00000433
TGGTCGG207.0332567E-445.0000041
TGCCGCG207.0332567E-445.0000041
ACGGTCC207.0332567E-445.00000415
GCGCGAC700.045.08
CCTACGG253.8907827E-545.01
GCCGATT253.8907827E-545.08
CGCAGTA351.2119199E-745.036