FastQCFastQC Report
Thu 26 May 2016
SRR1043222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043222_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1878489
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT686273.653308590042316No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT91060.4847513080992223RNA PCR Primer, Index 41 (96% over 27bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC88860.4730397676004491No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT70270.3740772503858154RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT56750.3021045105933545Illumina PCR Primer Index 11 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA53090.2826207659453955No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33420.17790894703136403No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC32770.1744487191567265RNA PCR Primer, Index 41 (96% over 26bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30340.16151279033308152No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG28010.14910920425938082No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT25400.1352150584858362Illumina PCR Primer Index 11 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG253.4179902E-546.01
TATACGG1950.046.01
CCAGTCG253.4179902E-546.027
CCGTTAA206.312482E-446.029
CGTTTTT183400.045.17231
TCGTAGG2750.045.1636351
CGACGGT5800.044.8103527
ACGGGTA4200.044.3571434
CGGTCTA5800.044.01724230
ACGTAGG2100.043.8095251
TCACGAC6250.043.42424
CACGACG6050.043.33884425
AATACGG800.043.1251
GACGGTC6150.042.26016228
CTAACGG1100.041.818181
CTTGCGG4350.041.7701151
CCAACGG1050.041.6190451
GCGCGAC14050.041.580078
TACGGGT3600.041.527783
ACGGTCT6250.041.21629
Produced by FastQC (version 0.11.5)