FastQCFastQC Report
Thu 26 May 2016
SRR1043221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043221_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1840360
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650233.5331674237649158No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC87310.4744180486426569No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC86410.4695277011019583RNA PCR Primer, Index 41 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT70410.38258818926731725RNA PCR Primer, Index 41 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC57330.31151513834249817Illumina PCR Primer Index 11 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG44330.24087678497685233No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG32190.17491143037231846RNA PCR Primer, Index 41 (96% over 25bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC31460.17094481514486295No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29150.15839292312373668No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC25040.13606033602121323No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC23750.1290508378795453Illumina PCR Primer Index 11 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT253.8917227E-545.00000418
GGACGTA207.0343923E-445.0000048
GCCCGTA253.8917227E-545.00000431
ATTACGG1250.044.9999961
CAATGCG302.1662163E-644.99999629
CGACGGT5500.044.59090827
CGTTTTT174350.044.290221
CTTGCGG4500.044.01
TCACGAC6000.043.50000424
TATACGG1500.043.5000041
ACGGGTA3950.043.291144
CACGACG5900.042.71186425
CGGTCTA5800.042.67241730
GCGATAT850.042.3529438
GCGACGG1400.041.7857171
TCGTAGG2750.041.7272721
CACTTAC24300.041.3888936
CTAGCGG5800.041.1206931
TACGGGT3400.041.0294153
AGGGCGC6650.040.939855
Produced by FastQC (version 0.11.5)