##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043221_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1840360
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28408680910257	33.0	31.0	34.0	31.0	34.0
2	32.58461387989306	34.0	31.0	34.0	31.0	34.0
3	32.70054065508922	34.0	31.0	34.0	31.0	34.0
4	36.192022756417224	37.0	37.0	37.0	35.0	37.0
5	36.170193875111394	37.0	37.0	37.0	35.0	37.0
6	36.349785368080155	37.0	37.0	37.0	35.0	37.0
7	36.30400628137973	37.0	37.0	37.0	35.0	37.0
8	36.18932382794671	37.0	37.0	37.0	35.0	37.0
9	37.60562987676324	39.0	37.0	39.0	35.0	39.0
10	37.48379827859767	39.0	37.0	39.0	35.0	39.0
11	37.13371242582973	39.0	35.0	39.0	34.0	39.0
12	37.13366189223847	39.0	35.0	39.0	34.0	39.0
13	37.15217240105197	39.0	35.0	39.0	34.0	39.0
14	38.43490512725771	40.0	37.0	41.0	35.0	41.0
15	38.49703699276228	40.0	37.0	41.0	35.0	41.0
16	38.42547979743094	40.0	37.0	41.0	34.0	41.0
17	38.35528374883175	40.0	36.0	41.0	34.0	41.0
18	38.184409028668306	40.0	36.0	41.0	34.0	41.0
19	38.04545415027495	40.0	36.0	41.0	34.0	41.0
20	37.845395466104456	40.0	35.0	41.0	34.0	41.0
21	37.79657512660567	40.0	35.0	41.0	34.0	41.0
22	37.76120813319133	40.0	35.0	41.0	34.0	41.0
23	37.75144047903671	40.0	35.0	41.0	34.0	41.0
24	37.68521321915277	39.0	35.0	41.0	34.0	41.0
25	37.670823643200244	39.0	35.0	41.0	34.0	41.0
26	37.649339259710054	39.0	35.0	41.0	34.0	41.0
27	37.62500380360364	39.0	35.0	41.0	34.0	41.0
28	37.552670129757225	39.0	35.0	41.0	34.0	41.0
29	37.50236149449021	39.0	35.0	41.0	34.0	41.0
30	37.38756927992349	39.0	35.0	41.0	34.0	41.0
31	37.27153491708144	39.0	35.0	41.0	33.0	41.0
32	37.04465974048556	39.0	35.0	41.0	33.0	41.0
33	36.76043219804821	39.0	35.0	41.0	32.0	41.0
34	36.48852181095003	39.0	35.0	41.0	31.0	41.0
35	36.36565834945337	39.0	35.0	41.0	31.0	41.0
36	36.3313362602969	39.0	35.0	41.0	31.0	41.0
37	36.23513714707992	39.0	35.0	41.0	31.0	41.0
38	36.20153774261558	39.0	35.0	41.0	31.0	41.0
39	36.15378078202091	39.0	35.0	41.0	31.0	41.0
40	36.09966473950749	39.0	35.0	41.0	31.0	41.0
41	36.06412060683779	38.0	35.0	41.0	31.0	41.0
42	35.96768295333522	38.0	35.0	41.0	30.0	41.0
43	35.84405822773805	38.0	35.0	41.0	30.0	41.0
44	35.71375763437588	38.0	35.0	40.0	30.0	41.0
45	35.65976548066683	38.0	35.0	40.0	29.0	41.0
46	35.56499217544393	38.0	35.0	40.0	29.0	41.0
47	35.43103794909692	38.0	35.0	40.0	28.0	41.0
48	35.38982590362755	37.0	35.0	40.0	28.0	41.0
49	35.32987839335782	37.0	35.0	40.0	28.0	41.0
50	35.24846008389663	37.0	35.0	40.0	28.0	41.0
51	34.838395748657874	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	11.0
15	34.0
16	60.0
17	114.0
18	299.0
19	566.0
20	1047.0
21	1824.0
22	2876.0
23	4433.0
24	7607.0
25	13371.0
26	20680.0
27	26210.0
28	28743.0
29	29734.0
30	32835.0
31	37192.0
32	43248.0
33	56253.0
34	129583.0
35	209515.0
36	139249.0
37	145923.0
38	250304.0
39	658402.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.676095981221067	18.433132647960182	24.985981003716663	26.904790367102088
2	33.31945923623639	20.893031798126454	25.473168293160033	20.314340672477122
3	26.484872524940773	21.386141841813558	33.01522528201004	19.11376035123563
4	23.456769327740222	24.436957986480905	30.965245930144103	21.14102675563477
5	21.964506944293507	31.904736029907195	27.88823925753657	18.24251776826273
6	79.23835553913364	1.9435871242582972	15.911669455976005	2.90638788063205
7	79.69603773174813	1.7784020517724795	13.82626225303745	4.699297963441936
8	69.45450890043251	3.207198591579908	20.283966180529898	7.054326327457671
9	38.928850877002326	15.508052772283683	29.443641461453197	16.119454889260798
10	32.82509943706666	17.256134669303833	32.79803951400813	17.120726379621377
11	29.432013301745314	15.13350648786107	35.52049598991502	19.913984220478604
12	23.40639874807103	16.62131322132626	38.14204829489882	21.830239735703884
13	17.382522984633443	20.397748266643482	40.66448955639114	21.555239192331936
14	19.024430002825536	19.7891173466061	40.43333912930079	20.753113521267576
15	24.007748483992263	15.760177356604144	40.26141624464778	19.97065791475581
16	21.31860070855702	21.517692190658348	35.94611923754048	21.217587863244148
17	22.843085048577453	20.737410071942445	36.356962768154055	20.062542111326046
18	21.1502097415723	27.696700645525873	34.30622269555956	16.84686691734226
19	22.746038818492035	22.839172770544895	32.45397639592254	21.960812015040535
20	21.176400269512484	28.288758721119784	32.898454650177136	17.636386359190592
21	18.896574583233715	21.79220369927623	36.26524158316851	23.045980134321546
22	19.536612401921364	22.6622508639614	33.448999108870005	24.352137625247234
23	21.606696515899063	20.388945641070226	37.381816601099786	20.62254124193093
24	23.251646417005368	18.870818752852703	33.61679236671086	24.260742463431068
25	16.57284444347845	21.160044773848593	36.60729422504293	25.659816557630027
26	16.292355843421937	18.908420091721183	36.95711708578756	27.842106979069314
27	19.656751939837857	22.337857810428392	35.766154448042776	22.239235801690974
28	21.634897520050426	23.169597252711426	31.471940272555372	23.72356495468278
29	21.649785911452106	20.90183442369971	35.23218283379339	22.216196831054795
30	24.900345584559545	20.456323762742073	30.223760568584407	24.41957008411398
31	26.982112195440024	20.050370579669195	31.471016540242125	21.496500684648655
32	21.992110239301006	22.239942185224628	32.1301810515334	23.637766523940968
33	19.19059314481949	19.983970527505488	34.906050990023694	25.91938533765133
34	19.748147101654023	25.554619748310113	31.499923927927142	23.197309222108718
35	19.907898454650176	27.62035688669608	30.900530331022193	21.571214327631548
36	20.947042969853726	26.88435958182095	27.04008998239475	25.12850746593058
37	20.06569366863005	28.100969375556957	30.326512204133977	21.50682475167902
38	19.394574974461516	25.126660001304092	28.790943076354626	26.687821947879765
39	20.951226933860767	20.225608033210893	33.172640135625635	25.650524897302702
40	16.373807298572018	20.603794909691583	35.33792301506227	27.68447477667413
41	18.419657023625813	23.342009172118498	30.13986393966398	28.098469864591713
42	18.536699341433195	18.57527874980982	33.87098176443739	29.01704014431959
43	21.898704601273664	19.544056597622202	32.80955899932622	25.747679801777913
44	19.386696081201503	22.246353974222437	32.71582733812949	25.651122606446563
45	20.522017431372124	21.71884848616575	32.38415310048034	25.374980981981786
46	21.49188202308244	20.55902106109674	32.196635440892	25.75246147492882
47	17.539340129105174	20.482133930317982	36.2085678888913	25.76995805168554
48	19.766404399139297	18.06363972266296	32.727401160642486	29.442554717555257
49	19.571116520680736	17.506194440218216	36.63419113651677	26.28849790258428
50	18.0889065182899	17.483590167141212	37.46853876415484	26.95896455041405
51	16.299691364732986	16.61310830489687	36.02871177378339	31.058488556586756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	728.0
1	3203.0
2	5678.0
3	49883.0
4	94088.0
5	53961.0
6	13834.0
7	12108.0
8	10382.0
9	10307.0
10	10232.0
11	10113.5
12	9995.0
13	9445.5
14	8896.0
15	8246.5
16	7597.0
17	7056.5
18	6516.0
19	6037.5
20	5559.0
21	5371.5
22	5184.0
23	4653.0
24	4122.0
25	4752.5
26	6343.0
27	7303.0
28	8496.5
29	9690.0
30	11033.5
31	12377.0
32	14246.5
33	16116.0
34	19086.0
35	22056.0
36	24170.5
37	26285.0
38	30101.5
39	33918.0
40	40223.5
41	46529.0
42	54609.5
43	62690.0
44	75509.5
45	88329.0
46	114408.0
47	140487.0
48	177763.5
49	215040.0
50	221373.0
51	227706.0
52	195982.5
53	164259.0
54	146424.0
55	128589.0
56	114779.5
57	100970.0
58	91848.0
59	82726.0
60	76375.0
61	70024.0
62	66336.5
63	62649.0
64	57493.5
65	52338.0
66	41185.0
67	30032.0
68	23410.0
69	16788.0
70	14523.5
71	12259.0
72	11094.0
73	9929.0
74	8315.5
75	5191.0
76	3680.0
77	2495.0
78	1310.0
79	995.5
80	681.0
81	540.5
82	400.0
83	293.0
84	186.0
85	117.5
86	49.0
87	34.0
88	19.0
89	24.0
90	29.0
91	19.0
92	9.0
93	9.5
94	10.0
95	5.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1840360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.369394267124786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69805706789462	20.056610548787905
2	12.312391316936113	6.985901672825687
3	4.925874248749143	4.1923220601912465
4	2.4822453738799126	2.8167919071738328
5	1.4307418222598602	2.029463942507727
6	0.9438992215398965	1.6066709498578493
7	0.6388194936240369	1.268604545610172
8	0.5108615597856616	1.1594266404382219
9	0.3999919821670989	1.0212777221208458
>10	4.998295709969187	32.39868766066974
>50	0.48232112075713796	8.997696744989607
>100	0.16200210997051648	8.072493531999765
>500	0.008119424581443437	1.58722888374958
>1k	0.005412949720962292	2.587411778360723
>5k	7.732785315660417E-4	1.653315432414735
>10k+	1.9331963289151042E-4	3.5660959783023713
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65023	3.5331674237649158	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8731	0.4744180486426569	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	8641	0.4695277011019583	RNA PCR Primer, Index 41 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	7041	0.38258818926731725	RNA PCR Primer, Index 41 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	5733	0.31151513834249817	Illumina PCR Primer Index 11 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4433	0.24087678497685233	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	3219	0.17491143037231846	RNA PCR Primer, Index 41 (96% over 25bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3146	0.17094481514486295	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2915	0.15839292312373668	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	2504	0.13606033602121323	No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2375	0.1290508378795453	Illumina PCR Primer Index 11 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39128214045078136	0.0
2	0.0	0.0	0.0	1.3942380838531592	0.0
3	0.0	0.0	0.0	2.2365732791410378	0.0
4	0.0	0.0	0.0	2.794561933534743	0.0
5	0.0	0.0	0.0	5.651122606446565	0.0
6	0.0	0.0	0.0	6.339194505422852	0.0
7	0.0	0.0	0.0	8.721989176030776	0.0
8	5.4337194896650656E-5	0.0	0.0	11.604414353713404	0.0
9	5.4337194896650656E-5	0.0	0.0	14.111749875024453	0.0
10	5.4337194896650656E-5	0.0	0.0	15.663837510052382	0.0
11	5.4337194896650656E-5	0.0	0.0	17.067584603012453	0.0
12	5.4337194896650656E-5	0.0	0.0	18.10591406029255	0.0
13	5.4337194896650656E-5	0.0	0.0	18.624345236801496	0.0
14	5.4337194896650656E-5	0.0	0.0	18.987046012736638	0.0
15	5.4337194896650656E-5	0.0	0.0	20.07058401617075	0.0
16	5.4337194896650656E-5	0.0	0.0	21.26996891912452	0.0
17	5.4337194896650656E-5	0.0	0.0	22.61584689951966	0.0
18	5.4337194896650656E-5	0.0	0.0	23.469158208176662	0.0
19	5.4337194896650656E-5	0.0	0.0	24.384142233041363	0.0
20	1.0867438979330131E-4	0.0	0.0	25.385033363037667	0.0
21	1.6301158468995196E-4	0.0	0.0	26.3140363841857	0.0
22	2.716859744832533E-4	0.0	0.0	27.18397487448108	0.0
23	2.716859744832533E-4	0.0	0.0	27.98800234736682	0.0
24	3.260231693799039E-4	0.0	0.0	28.683409767654155	0.0
25	3.260231693799039E-4	0.0	0.0	29.32888130583147	0.0
26	3.260231693799039E-4	0.0	0.0	29.94745593253494	0.0
27	3.260231693799039E-4	0.0	0.0	30.606729118216002	0.0
28	3.260231693799039E-4	0.0	0.0	31.2508422265209	0.0
29	3.260231693799039E-4	0.0	0.0	31.915223108522245	0.0
30	3.260231693799039E-4	0.0	0.0	32.53705796691951	0.0
31	3.260231693799039E-4	0.0	0.0	33.10558803712317	0.0
32	3.260231693799039E-4	0.0	0.0	33.790291030015865	0.0
33	3.260231693799039E-4	0.0	0.0	34.41495142254776	0.0
34	3.260231693799039E-4	0.0	0.0	35.084548675259185	0.0
35	3.260231693799039E-4	0.0	0.0	35.65802343019844	0.0
36	3.260231693799039E-4	0.0	0.0	36.228237953443895	0.0
37	3.260231693799039E-4	0.0	0.0	37.01183464104849	0.0
38	3.260231693799039E-4	0.0	0.0	37.75984046599579	0.0
39	3.260231693799039E-4	0.0	0.0	38.612825751483406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	25	3.8917227E-5	45.000004	18
GGACGTA	20	7.0343923E-4	45.000004	8
GCCCGTA	25	3.8917227E-5	45.000004	31
ATTACGG	125	0.0	44.999996	1
CAATGCG	30	2.1662163E-6	44.999996	29
CGACGGT	550	0.0	44.590908	27
CGTTTTT	17435	0.0	44.29022	1
CTTGCGG	450	0.0	44.0	1
TCACGAC	600	0.0	43.500004	24
TATACGG	150	0.0	43.500004	1
ACGGGTA	395	0.0	43.29114	4
CACGACG	590	0.0	42.711864	25
CGGTCTA	580	0.0	42.672417	30
GCGATAT	85	0.0	42.352943	8
GCGACGG	140	0.0	41.785717	1
TCGTAGG	275	0.0	41.727272	1
CACTTAC	2430	0.0	41.38889	36
CTAGCGG	580	0.0	41.120693	1
TACGGGT	340	0.0	41.029415	3
AGGGCGC	665	0.0	40.93985	5
>>END_MODULE