SRR1043220_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043220_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1390407
Sequences flagged as poor quality	0
Sequence length	52
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62914	4.524862144681378	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	7191	0.5171866942557107	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	6258	0.4500840401407646	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6022	0.43311059279764846	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	5623	0.4044139593658547	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4203	0.30228558975896985	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2759	0.1984311068629545	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	2418	0.17390591387989274	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	2408	0.17318670000942168	Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2133	0.15340831857146864	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	2125	0.15283294747509182	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2108	0.1516102838952911	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	1979	0.14233242496621493	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1868	0.13434915100398662	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1776	0.1277323833956532	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	1703	0.12248212214121476	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1531	0.11011164356911321	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1392	0.10011457076956602	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	35	1.01978E-7	46.000004	26
AACGTAC	20	6.311914E-4	46.0	37
TTCGAAT	45	3.110472E-10	46.0	15
TCGAATG	30	1.8616865E-6	46.0	16
CCGTTCA	20	6.311914E-4	46.0	31
CGTTTTT	13880	0.0	45.287464	1
CATGCGG	230	0.0	45.0	1
ACGGGTA	140	0.0	44.357143	4
TCACGAC	515	0.0	44.213593	24
ACGGTCT	495	0.0	43.676765	29
ATCACGG	90	0.0	43.444447	1
AGGGTAA	695	0.0	43.352516	5
TAGTCAG	260	0.0	43.346153	33
CACGAGG	160	0.0	43.125	1
TAGTAGG	300	0.0	42.93333	1
CGACGGT	505	0.0	42.81188	27
TAGGGCA	210	0.0	42.714283	4
CACTTAC	1760	0.0	42.602272	36
TAGGGTA	805	0.0	42.571426	4
TGTTAGG	200	0.0	42.55	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content