##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043220_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1390407
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25065754128108	33.0	31.0	34.0	31.0	34.0
2	32.59360101035165	34.0	31.0	34.0	31.0	34.0
3	32.724821581019086	34.0	31.0	34.0	31.0	34.0
4	36.17607650134097	37.0	37.0	37.0	35.0	37.0
5	36.08121003418424	37.0	35.0	37.0	35.0	37.0
6	36.26686718349375	37.0	37.0	37.0	35.0	37.0
7	36.21181639620629	37.0	37.0	37.0	35.0	37.0
8	36.1917977973356	37.0	37.0	37.0	35.0	37.0
9	37.7378566132075	39.0	38.0	39.0	35.0	39.0
10	37.6026285828538	39.0	37.0	39.0	35.0	39.0
11	37.36871362126342	39.0	37.0	39.0	35.0	39.0
12	37.37250963207176	39.0	37.0	39.0	35.0	39.0
13	37.25444851759233	39.0	37.0	39.0	34.0	39.0
14	38.737358917209136	41.0	38.0	41.0	35.0	41.0
15	38.84157947996522	41.0	38.0	41.0	35.0	41.0
16	38.810522386610536	41.0	38.0	41.0	35.0	41.0
17	38.795318924602654	41.0	38.0	41.0	35.0	41.0
18	38.55910247862676	40.0	38.0	41.0	35.0	41.0
19	38.289298744899874	40.0	37.0	41.0	34.0	41.0
20	38.03436547715884	40.0	35.0	41.0	34.0	41.0
21	37.97874938776919	40.0	35.0	41.0	34.0	41.0
22	37.901000929943535	40.0	35.0	41.0	34.0	41.0
23	37.89457115794152	40.0	35.0	41.0	34.0	41.0
24	37.8151023405377	40.0	35.0	41.0	34.0	41.0
25	37.68478582170545	40.0	35.0	41.0	34.0	41.0
26	37.623388691224946	40.0	35.0	41.0	34.0	41.0
27	37.63708899624355	40.0	35.0	41.0	34.0	41.0
28	37.593683719946746	40.0	35.0	41.0	33.0	41.0
29	37.546371673905554	40.0	35.0	41.0	33.0	41.0
30	37.51341225986348	40.0	35.0	41.0	33.0	41.0
31	37.43329255390688	40.0	35.0	41.0	33.0	41.0
32	37.236316416703886	40.0	35.0	41.0	33.0	41.0
33	37.016439071437354	40.0	35.0	41.0	33.0	41.0
34	36.836270962387275	40.0	35.0	41.0	32.0	41.0
35	36.66885595368838	40.0	35.0	41.0	31.0	41.0
36	36.54024828701236	39.0	35.0	41.0	31.0	41.0
37	36.50786712092215	39.0	35.0	41.0	31.0	41.0
38	36.372490213297255	39.0	35.0	41.0	31.0	41.0
39	36.2966196228874	39.0	35.0	41.0	31.0	41.0
40	36.20831742072645	39.0	35.0	41.0	31.0	41.0
41	36.19068733111959	39.0	35.0	41.0	31.0	41.0
42	36.10821148052333	39.0	35.0	41.0	31.0	41.0
43	36.01559327592568	38.0	35.0	41.0	30.0	41.0
44	35.864344037393366	38.0	35.0	41.0	30.0	41.0
45	35.787455759356796	38.0	35.0	41.0	30.0	41.0
46	35.726144215326876	38.0	35.0	40.0	30.0	41.0
47	35.61980269086678	38.0	35.0	40.0	30.0	41.0
48	35.526371055381624	37.0	35.0	40.0	29.0	41.0
49	35.4919027306393	37.0	35.0	40.0	29.0	41.0
50	35.38056914270426	37.0	35.0	40.0	29.0	41.0
51	35.271086092057935	37.0	35.0	40.0	28.0	41.0
52	34.90540036118921	36.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	2.0
14	6.0
15	17.0
16	41.0
17	107.0
18	242.0
19	472.0
20	877.0
21	1486.0
22	2373.0
23	3752.0
24	6471.0
25	11379.0
26	16707.0
27	19460.0
28	20277.0
29	20616.0
30	21824.0
31	25293.0
32	31292.0
33	41518.0
34	83063.0
35	119365.0
36	129913.0
37	132391.0
38	204531.0
39	495863.0
40	1066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.489202082555682	17.47179063396545	24.128546533497026	27.91046074998184
2	32.61354409176593	20.938976860732144	25.59639012174133	20.851088925760585
3	25.52080074395483	21.049376189849447	33.70602996101141	19.72379310518431
4	23.49585409164367	23.644155991734795	31.97481025340062	20.885179663220914
5	21.157689798742382	31.707478457746546	28.75323556339978	18.38159618011129
6	76.7705427259788	2.156562790607355	17.86707057717632	3.205823906237526
7	77.36080154947437	1.9936608489456684	15.303792342817607	5.341745258762362
8	67.04044211515046	3.9677590806145253	21.56749786213677	7.424300942098248
9	38.359775231281205	16.825433128573145	26.083010226502022	18.73178141364363
10	25.050506794053828	17.13728426280938	36.58432387063644	21.227885072500357
11	21.0691545712874	15.180015635709543	41.15464033193159	22.596189461071468
12	19.287877578291823	17.578593893730396	43.239281735491836	19.89424679248594
13	14.9590012133138	21.33698981665081	43.21267082228441	20.49133814775098
14	13.09465501827882	19.15395995561012	44.0268209236576	23.724564102453456
15	16.51286278046644	15.444182890333549	43.41167730024374	24.63127702895627
16	17.801765957737555	19.71106301967697	36.24492684516116	26.242244177424308
17	18.211574021131945	19.82973330830469	37.07712921468318	24.881563455880183
18	20.357923974778608	23.75692872662465	35.64726011879974	20.237887179796996
19	19.44186126795967	20.679124889330964	33.51925011885009	26.359763723859274
20	18.5886578534199	24.481249015576015	36.10122791384106	20.82886521716303
21	18.54543309980459	18.997530938782674	35.70479722843743	26.752238732975307
22	17.134910857036825	21.91595698237998	33.711280222265856	27.23785193831734
23	16.8885801064005	21.755931896200178	38.35991907405529	22.995568923344027
24	22.866829640529716	18.891518814275244	34.544273727045386	23.69737781814965
25	17.015593275925685	21.775062985154705	34.3789264582241	26.830417280695507
26	17.253509224277497	19.652662853394727	35.11935713787402	27.974470784453757
27	21.808937958453892	18.627926930747616	35.280532966246575	24.28260214455192
28	17.68870553729951	22.014633125408604	33.5400353997067	26.75662593758518
29	19.68286983595451	20.199696923274985	33.59073997757491	26.526693263195593
30	21.23047424243405	20.56534525502245	30.997973974526882	27.20620652801662
31	21.554552012468292	19.295717009479958	32.35549015504093	26.794240823010817
32	20.853678095694285	21.24615310481032	28.839756992017445	29.060411807477955
33	17.29601476402233	18.724373510777777	36.767939171767686	27.211672553432198
34	18.46279542608747	19.369292588429143	33.17266095467011	28.99525103081328
35	23.274408140925644	20.524709671340837	32.84124720315706	23.35963498457646
36	18.037811950026146	21.47435966591077	34.32124550581233	26.166582878250754
37	21.919337287571196	21.232847648206604	33.6791313622558	23.1686837019664
38	19.5503187196267	20.277659706834044	32.11009438243622	28.061927191103038
39	23.30526241596885	18.251706155104223	36.07044556018489	22.372585868742032
40	18.7474602760199	16.54954268786046	37.05878926098617	27.644207775133466
41	20.04146987177136	21.546712581280158	30.905770756332494	27.506046790615983
42	19.51680335326275	17.780477227171612	35.40064168261524	27.3020777369504
43	22.241329337381067	19.720197035831955	35.02837658325944	23.01009704352754
44	18.45294219606202	21.16222084612635	33.87389447837935	26.51094247943228
45	21.271109826115662	18.45402101686772	33.64712634501984	26.627742811996775
46	22.41106381081223	18.61987173539834	33.10555830055516	25.863506153234265
47	18.321901428862198	20.777513346811403	35.37079430699069	25.529790917335717
48	20.020756512301794	17.697192260971068	33.39310000596947	28.888951220757665
49	20.06527585088395	16.883545609307202	36.9711890115628	26.079989528246045
50	19.545715750855685	17.199640105379217	36.68745913966198	26.567185004103116
51	18.318736887832124	16.73423680979742	35.464939402635345	29.482086899735116
52	17.904685462601957	17.32334489110023	39.53115886211735	25.24081078418046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	593.0
1	2771.5
2	4950.0
3	39411.5
4	73873.0
5	41698.5
6	9524.0
7	9053.0
8	8582.0
9	8557.5
10	8533.0
11	8264.5
12	7996.0
13	7718.5
14	6972.0
15	6503.0
16	6268.0
17	6033.0
18	5667.0
19	5301.0
20	4964.5
21	4628.0
22	4292.0
23	3956.0
24	4136.5
25	4317.0
26	4824.0
27	5331.0
28	5953.0
29	6575.0
30	6759.5
31	6944.0
32	8947.5
33	10951.0
34	12422.0
35	13893.0
36	16142.0
37	18391.0
38	19616.5
39	23211.5
40	25581.0
41	31524.0
42	37467.0
43	49875.5
44	62284.0
45	78211.5
46	94139.0
47	118405.5
48	142672.0
49	154323.0
50	165974.0
51	149006.0
52	132038.0
53	111999.5
54	91961.0
55	82741.0
56	73521.0
57	68675.5
58	63830.0
59	60853.0
60	57876.0
61	53122.5
62	48369.0
63	46557.0
64	37713.0
65	30681.0
66	26797.5
67	22914.0
68	19719.0
69	16524.0
70	15433.0
71	14342.0
72	14271.5
73	14201.0
74	11257.0
75	8313.0
76	6042.5
77	3772.0
78	2729.5
79	1687.0
80	1496.0
81	1305.0
82	966.0
83	627.0
84	465.0
85	303.0
86	189.5
87	76.0
88	51.5
89	18.5
90	10.0
91	8.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1390407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.24989103004877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79550332080636	21.292605396111398
2	11.187315642180472	6.544555269050778
3	4.476074211985388	3.927740488289521
4	2.2713226492864838	2.657437599428453
5	1.2906976869015419	1.8876383347303052
6	0.8809522694396393	1.5460654730270167
7	0.6232033604009927	1.2760041266902473
8	0.48569380904109155	1.1365192790736995
9	0.41259218323197394	1.086144875944664
>10	5.033904629969159	33.15758594435428
>50	0.3896224853767147	7.483202498290581
>100	0.13901805544353837	7.404867446108558
>500	0.0076682556848777835	1.50697730839096
>1k	0.005194624818788176	2.7249750042548646
>5k	9.89452346435843E-4	1.815637054770633
>10k+	2.4736308660896074E-4	4.552043901484004
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62914	4.524862144681378	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	7191	0.5171866942557107	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	6258	0.4500840401407646	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6022	0.43311059279764846	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	5623	0.4044139593658547	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4203	0.30228558975896985	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2759	0.1984311068629545	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	2418	0.17390591387989274	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	2408	0.17318670000942168	Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2133	0.15340831857146864	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	2125	0.15283294747509182	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2108	0.1516102838952911	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	1979	0.14233242496621493	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1868	0.13434915100398662	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1776	0.1277323833956532	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	1703	0.12248212214121476	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1531	0.11011164356911321	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1392	0.10011457076956602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46410871061494946	0.0
2	0.0	0.0	0.0	1.6854777054488361	0.0
3	0.0	0.0	0.0	2.704387995745131	0.0
4	0.0	0.0	0.0	3.332837075762708	0.0
5	0.0	0.0	0.0	6.532763428262372	0.0
6	0.0	0.0	0.0	7.263916248983211	0.0
7	0.0	0.0	0.0	9.934141585880969	0.0
8	0.0	0.0	0.0	12.873856360044217	0.0
9	0.0	0.0	0.0	15.157360398789708	0.0
10	0.0	0.0	0.0	16.711078123168253	0.0
11	0.0	0.0	0.0	18.28874566943348	0.0
12	0.0	0.0	0.0	19.4174799177507	0.0
13	0.0	0.0	0.0	19.930423250170634	0.0
14	0.0	0.0	0.0	20.269604511484765	0.0
15	0.0	0.0	0.0	21.399777187542927	0.0
16	0.0	0.0	0.0	22.662860586864134	0.0
17	0.0	0.0	0.0	24.200683684705268	0.0
18	0.0	0.0	0.0	24.986640602356	0.0
19	0.0	0.0	0.0	25.87465396822657	0.0
20	1.4384277409420408E-4	0.0	0.0	26.84206854539714	0.0
21	1.4384277409420408E-4	0.0	0.0	27.764963783985554	0.0
22	2.157641611413061E-4	0.0	0.0	28.692821598280215	0.0
23	2.8768554818840817E-4	0.0	0.0	29.438358696410475	0.0
24	6.472924834239184E-4	0.0	0.0	30.037895378835117	0.0
25	7.192138704710204E-4	0.0	0.0	30.613769924921264	0.0
26	7.192138704710204E-4	0.0	0.0	31.254805247672085	0.0
27	7.911352575181224E-4	0.0	0.0	31.85204044571122	0.0
28	7.911352575181224E-4	0.0	0.0	32.52817340534103	0.0
29	7.911352575181224E-4	0.0	0.0	33.18668562514429	0.0
30	7.911352575181224E-4	0.0	0.0	33.914242376512775	0.0
31	7.911352575181224E-4	0.0	0.0	34.468468585097746	0.0
32	7.911352575181224E-4	0.0	0.0	35.06095697159177	0.0
33	7.911352575181224E-4	0.0	0.0	35.67164146900871	0.0
34	7.911352575181224E-4	0.0	0.0	36.38208093025999	0.0
35	7.911352575181224E-4	0.0	0.0	36.92300168224124	0.0
36	8.630566445652244E-4	0.0	0.0	37.506643738128474	0.0
37	8.630566445652244E-4	0.0	0.0	38.06273990277667	0.0
38	8.630566445652244E-4	0.0	0.0	38.90263786071273	0.0
39	8.630566445652244E-4	0.0	0.0	39.4777931929284	0.0
40	8.630566445652244E-4	0.0	0.0	40.04043420379788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	35	1.01978E-7	46.000004	26
AACGTAC	20	6.311914E-4	46.0	37
TTCGAAT	45	3.110472E-10	46.0	15
TCGAATG	30	1.8616865E-6	46.0	16
CCGTTCA	20	6.311914E-4	46.0	31
CGTTTTT	13880	0.0	45.287464	1
CATGCGG	230	0.0	45.0	1
ACGGGTA	140	0.0	44.357143	4
TCACGAC	515	0.0	44.213593	24
ACGGTCT	495	0.0	43.676765	29
ATCACGG	90	0.0	43.444447	1
AGGGTAA	695	0.0	43.352516	5
TAGTCAG	260	0.0	43.346153	33
CACGAGG	160	0.0	43.125	1
TAGTAGG	300	0.0	42.93333	1
CGACGGT	505	0.0	42.81188	27
TAGGGCA	210	0.0	42.714283	4
CACTTAC	1760	0.0	42.602272	36
TAGGGTA	805	0.0	42.571426	4
TGTTAGG	200	0.0	42.55	1
>>END_MODULE