FastQCFastQC Report
Thu 26 May 2016
SRR1043219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043219_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1364036
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT601684.411027274939958No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC68540.502479406701876No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT60540.4438299282423631Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC56100.41127946769733353No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC55970.41032641367236644No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG35340.2590840710948978No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG26860.1969156239278142No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC23740.17404232732860422No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC22650.16605133588849563No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC21380.15674073118304793No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC20990.1538815691081467No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG20490.15021597670442716No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA19110.1400989416701612No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG18130.1329143805588709No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG18110.1327677568627221No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC17690.12968865924359768No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.10600893231556938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG302.1658016E-645.00000420
TTGCGTA207.03375E-445.00000436
TTAACGG900.045.0000041
CGTAAGA207.03375E-445.00000439
AATACGG302.1658016E-645.0000041
TACCGAA302.1658016E-645.0000048
GTCATAG253.8911898E-545.027
CATGCGG2150.045.01
ACGGGTA1550.045.04
TCATGCG253.8911898E-545.033
ATAACGG502.1827873E-1145.01
CGGTCTA5200.044.56730730
CGTTTTT133100.044.27311
CGACGGT5550.043.78378327
TAGTAGG3300.043.6363641
CACGACG5500.043.36363625
TCACGAC5900.043.0932224
ACGGTCT5500.042.95454429
ACGTAGG1050.042.8571431
ACGGGAT3050.042.7868844