##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043219_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1364036
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2359057972077	33.0	31.0	34.0	31.0	34.0
2	32.53531651657288	34.0	31.0	34.0	31.0	34.0
3	32.646120776870994	34.0	31.0	34.0	31.0	34.0
4	36.1483780486732	37.0	37.0	37.0	35.0	37.0
5	36.10543783301907	37.0	35.0	37.0	35.0	37.0
6	36.28836922192669	37.0	37.0	37.0	35.0	37.0
7	36.246095410971556	37.0	37.0	37.0	35.0	37.0
8	36.14457682935055	37.0	37.0	37.0	35.0	37.0
9	37.702738050901885	39.0	38.0	39.0	35.0	39.0
10	37.551095425633925	39.0	37.0	39.0	35.0	39.0
11	37.36152638200165	39.0	37.0	39.0	35.0	39.0
12	37.37686541997425	39.0	37.0	39.0	35.0	39.0
13	37.264739347055354	39.0	37.0	39.0	34.0	39.0
14	38.751363600374184	40.0	38.0	41.0	35.0	41.0
15	38.86454169831295	41.0	38.0	41.0	35.0	41.0
16	38.86567509948418	41.0	38.0	41.0	35.0	41.0
17	38.827641645821664	40.0	38.0	41.0	35.0	41.0
18	38.62118741734089	40.0	38.0	41.0	35.0	41.0
19	38.373260676404435	40.0	37.0	41.0	35.0	41.0
20	38.1428386054327	40.0	35.0	41.0	34.0	41.0
21	38.084213319883055	40.0	35.0	41.0	34.0	41.0
22	38.031667785894214	40.0	35.0	41.0	34.0	41.0
23	37.98888519071344	40.0	35.0	41.0	34.0	41.0
24	37.91293338298989	40.0	35.0	41.0	34.0	41.0
25	37.84204229213892	40.0	35.0	41.0	34.0	41.0
26	37.83607177523174	40.0	35.0	41.0	34.0	41.0
27	37.80483066429332	40.0	35.0	41.0	34.0	41.0
28	37.70987422619344	40.0	35.0	41.0	34.0	41.0
29	37.691420900914636	40.0	35.0	41.0	34.0	41.0
30	37.61351679867687	40.0	35.0	41.0	34.0	41.0
31	37.5224473547619	40.0	35.0	41.0	33.0	41.0
32	37.335533666266876	40.0	35.0	41.0	33.0	41.0
33	37.09949810708808	40.0	35.0	41.0	33.0	41.0
34	36.872147802550664	40.0	35.0	41.0	32.0	41.0
35	36.70543152820014	40.0	35.0	41.0	32.0	41.0
36	36.57606177549566	39.0	35.0	41.0	31.0	41.0
37	36.48750912732508	39.0	35.0	41.0	31.0	41.0
38	36.3700180933641	39.0	35.0	41.0	31.0	41.0
39	36.24409253128216	39.0	35.0	41.0	31.0	41.0
40	36.129321366884746	39.0	35.0	41.0	30.0	41.0
41	36.05714805181095	39.0	35.0	41.0	30.0	41.0
42	35.96074150535616	38.0	35.0	41.0	30.0	41.0
43	35.85819802409907	38.0	35.0	40.0	30.0	41.0
44	35.76666524930427	38.0	35.0	40.0	30.0	41.0
45	35.71746346870611	38.0	35.0	40.0	30.0	41.0
46	35.60367101748048	38.0	35.0	40.0	29.0	41.0
47	35.437277315261476	37.0	35.0	40.0	29.0	41.0
48	35.38059772615972	37.0	35.0	40.0	29.0	41.0
49	35.325706213032504	37.0	35.0	40.0	28.0	41.0
50	35.25047139518312	37.0	35.0	40.0	28.0	41.0
51	34.850820652827345	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	5.0
15	11.0
16	42.0
17	96.0
18	199.0
19	432.0
20	756.0
21	1228.0
22	2117.0
23	3321.0
24	5504.0
25	9982.0
26	15395.0
27	19140.0
28	20188.0
29	20661.0
30	21498.0
31	24880.0
32	29832.0
33	40000.0
34	82064.0
35	116192.0
36	123891.0
37	129648.0
38	212611.0
39	484218.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.783425070892555	17.552689225211065	24.17201598784783	27.49186971604855
2	32.35273849077297	21.01916664956057	25.543607353471607	21.08448750619485
3	25.4963945233117	21.14423666237548	33.421478612001444	19.937890202311376
4	23.652894791633067	23.860587257227813	31.53465157811084	20.951866373028277
5	21.262342049623324	31.685014178511416	28.576591820157237	18.47605195170802
6	77.03227774047019	2.118565785653751	17.615590790858892	3.2335656830171637
7	77.6714837438308	1.9827922430199791	15.012360377585344	5.333363635563871
8	67.24785856091775	3.918738215120422	21.348190223718436	7.485213000243396
9	38.544510555439885	16.84383696617978	25.807163447299047	18.804489031081292
10	25.3552692157685	17.054315282001355	36.24713717233269	21.34327832989745
11	21.272898955746037	15.120568665343143	40.95104528032984	22.655487098580977
12	19.437463527355582	17.576588887683318	43.0902116952925	19.8957358896686
13	15.009867774750813	21.32722303516916	43.1683621253398	20.494547064740225
14	13.136163561665528	19.07391007275468	44.02838341510048	23.761542950479313
15	16.515253263110356	15.512567116996912	43.36930990091171	24.602869718981026
16	17.884132090355386	19.610259553266925	36.12060092255629	26.385007433821393
17	18.349442390083546	19.732470404006932	36.92065312059212	24.997434085317398
18	20.511408789797336	23.75648443296218	35.482787844309094	20.24931893293139
19	19.46664164288919	20.721740481922765	33.311144280649486	26.50047359453856
20	18.597749619511507	24.566287106791904	35.918993340351726	20.916969933344866
21	18.61901005545308	19.025744188569803	35.55052799192983	26.804717764047282
22	17.11714353580111	21.89157764164582	33.467591764440236	27.523687058112834
23	16.92917195733837	21.820245213469438	38.17010694732397	23.08047588186822
24	22.93891070323657	18.824136606365226	34.39623294399855	23.840719746399657
25	16.927925655921104	21.85235580292602	34.26742402693184	26.95229451422103
26	17.269632179795842	19.613558586430273	35.01058623086194	28.106223002911946
27	21.86833778580624	18.56549240635878	35.085877498834336	24.480292309000642
28	17.694767586779232	22.006677243122617	33.289883844707916	27.00867132539024
29	19.694714802248622	20.202619285708	33.32324073558176	26.77942517646162
30	21.2801568287054	20.588972725060042	30.707547308135563	27.423323138098993
31	21.6382852065488	19.22397942576296	32.21146655953362	26.926268808154624
32	20.855607916506603	21.267327255292383	28.57167992633626	29.305384901864763
33	17.351814761487233	18.50713617529156	36.6490327234765	27.4920163397447
34	18.515493725972043	19.280429548780237	33.019583060857634	29.18449366439009
35	23.328269928359664	20.43897668389984	32.792389643675094	23.440363744065408
36	17.9937333032266	21.43125254758672	34.31918219167236	26.25583195751432
37	22.041940242046397	21.06615954417625	33.64390675905914	23.247993454718205
38	19.716341797430566	20.09052547000226	32.09753994762601	28.09559278494116
39	23.443002970596083	18.122029037356786	35.9276441384245	22.507323853622633
40	18.726411912882064	16.363057866507923	37.151732065722605	27.75879815488741
41	20.18135884976643	21.34144553369559	30.85211827253826	27.62507734399972
42	19.618470480251254	17.628347052423837	35.34254227894279	27.410640188382125
43	22.34750402482046	19.54977727860555	34.97393030682475	23.128788389749243
44	18.429865487421154	21.04643865704424	33.82879923990276	26.694896615631848
45	21.259336263852273	18.235222530783645	33.73356714925412	26.771874056109958
46	22.60277587981549	18.39159670272632	33.104918052016224	25.900709365441966
47	18.485069309021167	20.540660217179017	35.41526763223258	25.55900284156723
48	20.043385951690425	17.502910480368552	33.41920594471114	29.03449762322989
49	20.177473321818486	16.719426759997535	37.01031351078711	26.092786407396872
50	19.640830593913943	17.06355257485873	36.76515869082635	26.530458140400988
51	18.385805066728445	16.672947048318374	35.25559442712656	29.68565345782663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	536.0
1	2601.5
2	4667.0
3	37625.0
4	70583.0
5	40158.0
6	9733.0
7	9036.5
8	8340.0
9	8137.0
10	7934.0
11	7848.5
12	7763.0
13	7340.0
14	6917.0
15	6515.0
16	6113.0
17	5809.5
18	5506.0
19	5105.5
20	4705.0
21	4484.5
22	4264.0
23	4084.0
24	3904.0
25	4073.5
26	4511.5
27	4780.0
28	5698.5
29	6617.0
30	7256.5
31	7896.0
32	9491.5
33	11087.0
34	13702.5
35	16318.0
36	17420.5
37	18523.0
38	20529.0
39	22535.0
40	26584.0
41	30633.0
42	38856.0
43	47079.0
44	56539.0
45	65999.0
46	87091.5
47	108184.0
48	137352.5
49	166521.0
50	166177.0
51	165833.0
52	138228.5
53	110624.0
54	95406.0
55	80188.0
56	73037.0
57	65886.0
58	64197.5
59	62509.0
60	57495.0
61	52481.0
62	48046.5
63	43612.0
64	40688.0
65	37764.0
66	32262.0
67	26760.0
68	22497.0
69	18234.0
70	16840.0
71	15446.0
72	14576.0
73	13706.0
74	11819.0
75	7444.0
76	4956.0
77	3508.0
78	2060.0
79	1695.0
80	1330.0
81	1051.5
82	773.0
83	596.0
84	419.0
85	248.5
86	78.0
87	63.0
88	48.0
89	28.5
90	9.0
91	7.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1364036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.176166922882373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54628188218852	21.166224298292093
2	11.277507199298583	6.580688650414864
3	4.417952010646145	3.866967159596872
4	2.277507137832909	2.6579571368587613
5	1.344243712153549	1.96099394654135
6	0.9077283561788227	1.5890420424304177
7	0.6575429089714379	1.3429207169775828
8	0.5275227946232076	1.2312874489241683
9	0.4125221525603441	1.0832233664238613
>10	5.090783281750604	33.22269105544288
>50	0.3834396152192496	7.344043664408287
>100	0.14251461894751902	7.504356865442431
>500	0.008114711370756578	1.6006197053616573
>1k	0.005071694606722862	2.6131962648319194
>5k	0.0010143389213445723	1.7841797261161307
>10k+	2.535847303361431E-4	4.451607951936776
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60168	4.411027274939958	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	6854	0.502479406701876	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	6054	0.4438299282423631	Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5610	0.41127946769733353	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	5597	0.41032641367236644	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3534	0.2590840710948978	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2686	0.1969156239278142	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2374	0.17404232732860422	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	2265	0.16605133588849563	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2138	0.15674073118304793	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2099	0.1538815691081467	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2049	0.15021597670442716	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1911	0.1400989416701612	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1813	0.1329143805588709	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	1811	0.1327677568627221	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1769	0.12968865924359768	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1446	0.10600893231556938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45731930828805106	0.0
2	0.0	0.0	0.0	1.6775950194862892	0.0
3	0.0	0.0	0.0	2.708946098196822	0.0
4	0.0	0.0	0.0	3.359588749857042	0.0
5	0.0	0.0	0.0	6.635162121820832	0.0
6	0.0	0.0	0.0	7.381476735218132	0.0
7	0.0	0.0	0.0	10.078546314026903	0.0
8	7.3311848074391E-5	0.0	0.0	13.097381594034175	0.0
9	7.3311848074391E-5	0.0	0.0	15.446659765578035	0.0
10	7.3311848074391E-5	0.0	0.0	17.051676055470676	0.0
11	7.3311848074391E-5	0.0	0.0	18.65859845341325	0.0
12	7.3311848074391E-5	0.0	0.0	19.835693486095675	0.0
13	7.3311848074391E-5	0.0	0.0	20.356500854816147	0.0
14	7.3311848074391E-5	0.0	0.0	20.70209290663883	0.0
15	7.3311848074391E-5	0.0	0.0	21.85946705218924	0.0
16	7.3311848074391E-5	0.0	0.0	23.159359430396265	0.0
17	7.3311848074391E-5	0.0	0.0	24.73285162561692	0.0
18	7.3311848074391E-5	0.0	0.0	25.554824066226992	0.0
19	7.3311848074391E-5	0.0	0.0	26.480826019254625	0.0
20	1.46623696148782E-4	0.0	0.0	27.457046588213213	0.0
21	2.19935544223173E-4	0.0	0.0	28.40328261130938	0.0
22	2.19935544223173E-4	0.0	0.0	29.356263324428387	0.0
23	2.93247392297564E-4	0.0	0.0	30.120539340603912	0.0
24	3.66559240371955E-4	0.0	0.0	30.725728646458013	0.0
25	3.66559240371955E-4	0.0	0.0	31.29822086807093	0.0
26	3.66559240371955E-4	0.0	0.0	31.92965581553566	0.0
27	3.66559240371955E-4	0.0	0.0	32.54466890903173	0.0
28	3.66559240371955E-4	0.0	0.0	33.24113146573844	0.0
29	3.66559240371955E-4	0.0	0.0	33.894706591321636	0.0
30	4.39871088446346E-4	0.0	0.0	34.649158819855195	0.0
31	4.39871088446346E-4	0.0	0.0	35.224070332454566	0.0
32	4.39871088446346E-4	0.0	0.0	35.83329178995276	0.0
33	4.39871088446346E-4	0.0	0.0	36.45160391661217	0.0
34	4.39871088446346E-4	0.0	0.0	37.161629165212645	0.0
35	4.39871088446346E-4	0.0	0.0	37.70838892815146	0.0
36	4.39871088446346E-4	0.0	0.0	38.29634994970807	0.0
37	4.39871088446346E-4	0.0	0.0	38.86231741684237	0.0
38	4.39871088446346E-4	0.0	0.0	39.71295479004953	0.0
39	4.39871088446346E-4	0.0	0.0	40.29050552917958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	30	2.1658016E-6	45.000004	20
TTGCGTA	20	7.03375E-4	45.000004	36
TTAACGG	90	0.0	45.000004	1
CGTAAGA	20	7.03375E-4	45.000004	39
AATACGG	30	2.1658016E-6	45.000004	1
TACCGAA	30	2.1658016E-6	45.000004	8
GTCATAG	25	3.8911898E-5	45.0	27
CATGCGG	215	0.0	45.0	1
ACGGGTA	155	0.0	45.0	4
TCATGCG	25	3.8911898E-5	45.0	33
ATAACGG	50	2.1827873E-11	45.0	1
CGGTCTA	520	0.0	44.567307	30
CGTTTTT	13310	0.0	44.2731	1
CGACGGT	555	0.0	43.783783	27
TAGTAGG	330	0.0	43.636364	1
CACGACG	550	0.0	43.363636	25
TCACGAC	590	0.0	43.09322	24
ACGGTCT	550	0.0	42.954544	29
ACGTAGG	105	0.0	42.857143	1
ACGGGAT	305	0.0	42.786884	4
>>END_MODULE