FastQCFastQC Report
Thu 26 May 2016
SRR1043218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043218_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2235388
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1107484.954307708549925No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC142280.636489056933293No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA102870.46018856681703574No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT79690.3564929220341167TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT62870.28124871386980693TruSeq Adapter, Index 15 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC54370.2432239951185208TruSeq Adapter, Index 15 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT41480.18556062750627633TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT37580.1681139918439215TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC36930.16520621923352904No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34180.15290410434340704No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33230.14865428283591037No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT27680.12382637823948237No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG27660.12373690831300875No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG23690.10597712790799628TruSeq Adapter, Index 15 (95% over 22bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23410.10472454893736569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAA206.3127413E-446.08
CGTTTTT281950.045.2658271
AATACGG1350.044.29631
TACGGGT1800.043.4444473
TATACGG1150.042.01
CGGTCTA3450.041.99999630
CACTTAC37300.041.62198336
AGCACGG3650.041.589041
CACGACG3600.041.5277825
CTTACTG37200.041.17741838
CGCACTT37850.040.8956434
ACGCATA451.5896148E-840.8888923
GTTTTTT319600.040.8545042
CCGCACT37900.040.8416933
ACTTACT37850.040.7133437
GCACTTA38150.040.69462635
TCTACGG1700.040.5882341
CGTTCAG14000.040.5785738
GTACGGG5050.040.5346532
ACCCGCA38650.040.34670331
Produced by FastQC (version 0.11.5)