##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043218_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2235388
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40580471935968	33.0	31.0	34.0	31.0	34.0
2	32.74033366914379	34.0	31.0	34.0	31.0	34.0
3	32.856026336367556	34.0	31.0	34.0	31.0	34.0
4	36.275467614570715	37.0	37.0	37.0	35.0	37.0
5	36.22119739391998	37.0	37.0	37.0	35.0	37.0
6	36.349007867985335	37.0	37.0	37.0	35.0	37.0
7	36.29315402963602	37.0	37.0	37.0	35.0	37.0
8	36.285898913298276	37.0	37.0	37.0	35.0	37.0
9	37.88680220167595	39.0	38.0	39.0	35.0	39.0
10	37.71920892480411	39.0	37.0	39.0	35.0	39.0
11	37.42820619955015	39.0	37.0	39.0	35.0	39.0
12	37.435110593776116	39.0	37.0	39.0	35.0	39.0
13	37.42102489590174	39.0	37.0	39.0	35.0	39.0
14	38.806392447306685	41.0	38.0	41.0	35.0	41.0
15	38.8557297435613	41.0	38.0	41.0	35.0	41.0
16	38.753043319549	41.0	38.0	41.0	34.0	41.0
17	38.73483663686125	41.0	38.0	41.0	34.0	41.0
18	38.509925793642985	40.0	38.0	41.0	34.0	41.0
19	38.233970120623354	40.0	37.0	41.0	34.0	41.0
20	37.93283939969258	40.0	35.0	41.0	34.0	41.0
21	37.86347739184428	40.0	35.0	41.0	34.0	41.0
22	37.80079699810503	40.0	35.0	41.0	34.0	41.0
23	37.806683671917355	40.0	35.0	41.0	34.0	41.0
24	37.708216649637556	40.0	35.0	41.0	34.0	41.0
25	37.61205124121629	39.0	35.0	41.0	34.0	41.0
26	37.51970485660655	39.0	35.0	41.0	34.0	41.0
27	37.54873024280349	39.0	35.0	41.0	34.0	41.0
28	37.515100286840585	39.0	35.0	41.0	34.0	41.0
29	37.43439662376286	39.0	35.0	41.0	34.0	41.0
30	37.36996709296104	39.0	35.0	41.0	33.0	41.0
31	37.23891512345955	39.0	35.0	41.0	33.0	41.0
32	36.96462538047086	39.0	35.0	41.0	33.0	41.0
33	36.57078234293107	39.0	35.0	41.0	31.0	41.0
34	36.30253360937788	39.0	35.0	41.0	31.0	41.0
35	36.082414328071906	39.0	35.0	41.0	30.0	41.0
36	36.02051455944113	39.0	35.0	41.0	30.0	41.0
37	35.925856271931316	39.0	35.0	41.0	30.0	41.0
38	35.777002023809736	38.0	35.0	41.0	30.0	41.0
39	35.72751844422534	38.0	35.0	41.0	29.0	41.0
40	35.664524011044165	38.0	35.0	41.0	29.0	41.0
41	35.618043936891496	38.0	35.0	41.0	29.0	41.0
42	35.529153775541424	38.0	35.0	41.0	29.0	41.0
43	35.42284292480768	38.0	35.0	41.0	29.0	41.0
44	35.24745726468962	37.0	35.0	40.0	27.0	41.0
45	35.15293049797172	37.0	35.0	40.0	27.0	41.0
46	35.11116146279751	37.0	35.0	40.0	26.0	41.0
47	35.022777701231284	37.0	35.0	40.0	26.0	41.0
48	34.93521572093972	36.0	35.0	40.0	26.0	41.0
49	34.88442364368065	36.0	35.0	40.0	27.0	41.0
50	34.79027578210136	36.0	35.0	40.0	27.0	41.0
51	34.65300788945812	36.0	35.0	40.0	26.0	41.0
52	34.27923027232856	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	6.0
14	14.0
15	48.0
16	88.0
17	170.0
18	403.0
19	804.0
20	1585.0
21	2588.0
22	3973.0
23	6585.0
24	11849.0
25	21042.0
26	31913.0
27	38389.0
28	39133.0
29	37636.0
30	37430.0
31	41171.0
32	48372.0
33	64184.0
34	144458.0
35	211946.0
36	216099.0
37	214553.0
38	317114.0
39	741952.0
40	1879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.654533351704494	16.531134639713553	23.550005636605366	31.264326371976587
2	36.376235356009786	19.385046354368907	23.40551170535048	20.83320658427083
3	25.194597090080112	20.701417382575197	34.787517871617816	19.31646765572688
4	22.62287352352254	23.35071137538539	32.972128328504944	21.05428677258713
5	21.308560303625143	30.57522899827681	29.57218165258112	18.54402904551693
6	76.70645990763126	2.0089129940753017	18.373767775437642	2.9108593228558086
7	76.51432324052917	1.9125091482999816	17.033284602046713	4.539883009124143
8	67.88799080964915	3.3162922946709923	22.02221717214193	6.7734997235379275
9	40.87133866693388	15.166405116248274	26.664766921894547	17.297489294923295
10	27.039198564186616	16.233244519519655	35.93479073878897	20.792766177504753
11	23.967427578568014	13.121212066987923	41.07027504844796	21.841085305996096
12	20.057323381891646	14.308791136035445	43.08558514226613	22.548300339806783
13	16.129235729994075	17.34007697992474	44.57557256279447	21.955114727286716
14	16.402611090334208	17.345310970623444	44.224492571312005	22.027585367730346
15	20.128541443364643	14.323106324271222	43.42552612790263	22.122826104461506
16	19.488294649519457	18.2689537565738	39.198653656546426	23.044097937360313
17	19.984763271521544	19.515717181983618	38.528344967406106	21.971174579088732
18	20.92509219875923	24.43123967740723	35.0247473816626	19.618920742170936
19	21.6303388941875	20.785921728129527	34.30715383638098	23.276585541302
20	21.55263426304516	24.24044505920225	34.79306500705918	19.41385567069341
21	19.24498118447446	19.486907865659116	35.89757124937595	25.370539700490475
22	20.611723781285395	21.12756264236902	34.38159281520702	23.87912076113856
23	21.289816354028922	19.476216209445518	36.991072690736466	22.242894745789098
24	23.41629283149055	17.825719740823516	34.41478615792873	24.3432012697572
25	18.54291067143601	18.973350487700568	36.95492684044112	25.528812000422295
26	18.185523050137157	18.540316043568275	37.062872306731535	26.211288599563026
27	21.547042392640563	20.722174405517073	36.092347279309	21.638435922533358
28	22.076883297217307	21.45699091164487	32.389187022566105	24.076938768571722
29	21.525435405397182	18.667989628646122	36.33525812968487	23.471316836271825
30	24.245231700268587	20.944909787473136	31.59518616007601	23.214672352182262
31	25.421492823617196	18.973976777185886	31.505224149006793	24.09930625019012
32	23.283608930530182	19.89260924725372	31.46353116327009	25.36025065894601
33	18.761619906700762	23.601898193960064	34.41330990414192	23.22317199519725
34	21.083006618985163	22.89817248728185	31.44134262150463	24.577478272228355
35	21.157714007590627	25.120426521033483	30.520249728458772	23.20160974291711
36	23.341988057554214	25.904630426574716	27.87395297818544	22.879428537685627
37	23.186489325343075	25.634789128330294	30.535906965591657	20.642814580734978
38	22.072365065930388	23.032153702176085	29.825157869685263	25.070323362208264
39	22.776180242535077	21.618976213525347	34.324421532190385	21.28042201174919
40	18.91837121788253	22.072275596003916	35.52832886281934	23.48102432329421
41	20.376373139696554	23.909093186507217	31.466170526101063	24.248363147695166
42	19.91027955773226	19.42087905992159	33.075331888692254	27.5935094936539
43	22.162148137146662	20.72781101088491	33.26022149174998	23.84981936021845
44	22.08077523901891	21.286327026896448	33.05860101244169	23.574296721642956
45	22.220929878839826	21.567038921207416	32.51775530690869	23.694275893044072
46	22.482361003995727	20.249057434324598	32.78862550930756	24.479956052372113
47	20.10335565906232	21.684736609483455	34.26617661005606	23.945731121398165
48	21.11566314214803	20.46105642510383	31.51497637099242	26.90830406175572
49	20.304931403407373	18.492852247574024	35.523497486789765	25.678718862228838
50	20.058754900715222	18.66790015871965	36.14151100390626	25.13183393665887
51	19.071812141784783	17.529171669526722	36.015671552321116	27.38334463636738
52	17.532705731622432	18.679307574345035	38.407784241482915	25.38020245254963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	632.0
1	3554.0
2	6476.0
3	76050.5
4	145625.0
5	83172.5
6	20720.0
7	19048.0
8	17376.0
9	17113.5
10	16851.0
11	16341.5
12	15832.0
13	15117.0
14	13475.5
15	12549.0
16	11702.5
17	10856.0
18	9992.5
19	9129.0
20	8761.0
21	8393.0
22	8068.0
23	7743.0
24	7760.0
25	7777.0
26	8465.0
27	9153.0
28	9462.5
29	9772.0
30	10905.0
31	12038.0
32	14004.5
33	15971.0
34	18446.0
35	20921.0
36	23954.0
37	26987.0
38	28885.5
39	35035.5
40	39287.0
41	49936.5
42	60586.0
43	83077.0
44	105568.0
45	135964.0
46	166360.0
47	182730.0
48	199100.0
49	194389.0
50	189678.0
51	171305.0
52	152932.0
53	136777.5
54	120623.0
55	115448.0
56	110273.0
57	109728.0
58	109183.0
59	107628.0
60	106073.0
61	105870.5
62	105668.0
63	105808.5
64	90596.5
65	75244.0
66	63439.5
67	51635.0
68	43018.5
69	34402.0
70	30238.0
71	26074.0
72	25358.5
73	24643.0
74	19783.0
75	14923.0
76	11877.0
77	8831.0
78	6546.0
79	4261.0
80	3270.5
81	2280.0
82	1642.0
83	1004.0
84	691.0
85	378.0
86	311.5
87	245.0
88	169.5
89	79.0
90	64.0
91	46.0
92	28.0
93	18.5
94	9.0
95	6.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2235388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.306627506684052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01251796986345	21.983639224812322
2	9.88534604908787	5.794123088705782
3	3.507945251380836	3.0841813438817796
4	1.7183900853328038	2.0144087256811005
5	1.121408439341708	1.6432349707319667
6	0.8380028843183627	1.473542302814706
7	0.6582497855131991	1.3503756889273
8	0.537607794183961	1.2604377095071528
9	0.4642390370351762	1.2244752479206449
>10	5.791429975322485	36.623449963486124
>50	0.3246381886078311	6.2919555707883115
>100	0.12917874254205158	6.568363378122187
>500	0.005522898735102449	1.1046776831098009
>1k	0.004602415612585374	2.616247227519711
>5k	4.602415612585374E-4	0.8854056822743714
>10k+	4.602415612585374E-4	6.081482191716767
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110748	4.954307708549925	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14228	0.636489056933293	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	10287	0.46018856681703574	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	7969	0.3564929220341167	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT	6287	0.28124871386980693	TruSeq Adapter, Index 15 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	5437	0.2432239951185208	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	4148	0.18556062750627633	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	3758	0.1681139918439215	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	3693	0.16520621923352904	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3418	0.15290410434340704	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3323	0.14865428283591037	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	2768	0.12382637823948237	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	2766	0.12373690831300875	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	2369	0.10597712790799628	TruSeq Adapter, Index 15 (95% over 22bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2341	0.10472454893736569	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2901062365906948	0.0
2	0.0	0.0	0.0	0.9445787487451843	0.0
3	0.0	0.0	0.0	1.6220897669666294	0.0
4	0.0	0.0	0.0	2.088317553820634	0.0
5	0.0	0.0	0.0	4.324797305881574	0.0
6	0.0	0.0	0.0	5.230098756904842	0.0
7	0.0	0.0	0.0	7.610222475919169	0.0
8	0.0	0.0	0.0	10.242830327442038	0.0
9	0.0	0.0	0.0	12.829942721353072	0.0
10	0.0	0.0	0.0	14.201471959230345	0.0
11	0.0	0.0	0.0	15.30154944018667	0.0
12	0.0	0.0	0.0	16.08083249977185	0.0
13	0.0	0.0	0.0	16.495122994307923	0.0
14	0.0	0.0	0.0	16.754719985971114	0.0
15	0.0	0.0	0.0	17.385930317242465	0.0
16	4.4734963236807213E-5	0.0	0.0	18.10115290947254	0.0
17	4.4734963236807213E-5	0.0	0.0	19.002965033363335	0.0
18	1.3420488971042165E-4	0.0	0.0	19.51974332867493	0.0
19	1.7893985294722885E-4	0.0	0.0	20.10460823803295	0.0
20	1.7893985294722885E-4	0.0	0.0	20.725082178127465	0.0
21	1.7893985294722885E-4	0.0	0.0	21.372844445796435	0.0
22	2.2367481618403606E-4	0.0	0.0	21.991976336993847	0.0
23	2.2367481618403606E-4	0.0	0.0	22.511975549658494	0.0
24	2.684097794208433E-4	0.0	0.0	22.959593591805987	0.0
25	3.578797058944577E-4	0.0	0.0	23.396072628107515	0.0
26	4.026146691312649E-4	0.0	0.0	23.836309401320936	0.0
27	4.026146691312649E-4	0.0	0.0	24.291845531961343	0.0
28	4.473496323680721E-4	0.0	0.0	24.736779476314627	0.0
29	4.473496323680721E-4	0.0	0.0	25.192136667102087	0.0
30	4.473496323680721E-4	0.0	0.0	25.63568382759503	0.0
31	4.473496323680721E-4	0.0	0.0	26.06088965316088	0.0
32	4.473496323680721E-4	0.0	0.0	26.533469804794514	0.0
33	4.473496323680721E-4	0.0	0.0	27.001621195067703	0.0
34	4.473496323680721E-4	0.0	0.0	27.50887094320986	0.0
35	4.473496323680721E-4	0.0	0.0	27.941905387342153	0.0
36	4.473496323680721E-4	0.0	0.0	28.3940863957398	0.0
37	4.473496323680721E-4	0.0	0.0	28.95157350759689	0.0
38	4.473496323680721E-4	0.0	0.0	29.557240174860024	0.0
39	4.473496323680721E-4	0.0	0.0	30.19046357947703	0.0
40	4.473496323680721E-4	0.0	0.0	30.779980925011674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGAA	20	6.3127413E-4	46.0	8
CGTTTTT	28195	0.0	45.265827	1
AATACGG	135	0.0	44.2963	1
TACGGGT	180	0.0	43.444447	3
TATACGG	115	0.0	42.0	1
CGGTCTA	345	0.0	41.999996	30
CACTTAC	3730	0.0	41.621983	36
AGCACGG	365	0.0	41.58904	1
CACGACG	360	0.0	41.52778	25
CTTACTG	3720	0.0	41.177418	38
CGCACTT	3785	0.0	40.89564	34
ACGCATA	45	1.5896148E-8	40.88889	23
GTTTTTT	31960	0.0	40.854504	2
CCGCACT	3790	0.0	40.84169	33
ACTTACT	3785	0.0	40.71334	37
GCACTTA	3815	0.0	40.694626	35
TCTACGG	170	0.0	40.588234	1
CGTTCAG	1400	0.0	40.57857	38
GTACGGG	505	0.0	40.534653	2
ACCCGCA	3865	0.0	40.346703	31
>>END_MODULE