FastQCFastQC Report
Thu 26 May 2016
SRR1043217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043217_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2201980
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1049274.765120482474863No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC135300.6144469977020681No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG87080.39546226577898075No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC81200.3687590259675383TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT63880.2901025440739698TruSeq Adapter, Index 15 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG55850.25363536453555435TruSeq Adapter, Index 15 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC41320.1876492974504764No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC38280.17384354081326805TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT37020.1681214179965304No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33670.152907837491712No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC31840.14459713530549778No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT26910.12220819444318295No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC26310.11948337405426025No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT23640.10735792332355425TruSeq Adapter, Index 15 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC22070.10022797663920653No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT207.0346956E-445.040
TGCGATA207.0346956E-445.037
CGACTTA207.0346956E-445.015
CGTTTTT265100.044.202191
ATAACGG850.042.3529431
TCGGGTA800.042.18754
TATACGG700.041.7857131
CACTTAC32900.041.238636
TGCATGG4700.041.170211
GCATAGG4700.041.170211
CGCACTT33050.041.05143734
AGGGTAC6150.040.9756135
TATGCGG4400.040.9090921
ACTACGG1100.040.9090921
GCACTTA33350.040.6821635
ACTTACT33500.040.5671637
CCGCACT33400.040.5538933
CTTACTG33300.040.47297338
CCCGCAC34350.039.95633332
ACCCGCA34050.039.91189631
Produced by FastQC (version 0.11.5)