##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043217_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2201980
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39043451802468	33.0	31.0	34.0	31.0	34.0
2	32.69100990926348	34.0	31.0	34.0	31.0	34.0
3	32.782708743948625	34.0	31.0	34.0	31.0	34.0
4	36.25517670460222	37.0	37.0	37.0	35.0	37.0
5	36.244779244134826	37.0	37.0	37.0	35.0	37.0
6	36.3735901325171	37.0	37.0	37.0	35.0	37.0
7	36.331240065759	37.0	37.0	37.0	35.0	37.0
8	36.246901879217795	37.0	37.0	37.0	35.0	37.0
9	37.854707127221864	39.0	38.0	39.0	35.0	39.0
10	37.671727717781266	39.0	37.0	39.0	35.0	39.0
11	37.42660560041417	39.0	37.0	39.0	35.0	39.0
12	37.44279375834476	39.0	37.0	39.0	35.0	39.0
13	37.43787636581622	39.0	37.0	39.0	35.0	39.0
14	38.81994023560614	41.0	38.0	41.0	35.0	41.0
15	38.87931498015423	41.0	38.0	41.0	35.0	41.0
16	38.810106358822516	41.0	38.0	41.0	35.0	41.0
17	38.76650105813859	41.0	38.0	41.0	34.0	41.0
18	38.56171218630505	40.0	38.0	41.0	35.0	41.0
19	38.312382492120726	40.0	37.0	41.0	35.0	41.0
20	38.034728744130284	40.0	35.0	41.0	34.0	41.0
21	37.96733258249394	40.0	35.0	41.0	34.0	41.0
22	37.91452374680969	40.0	35.0	41.0	34.0	41.0
23	37.8911456961462	40.0	35.0	41.0	34.0	41.0
24	37.795022207286166	40.0	35.0	41.0	34.0	41.0
25	37.75900734793232	40.0	35.0	41.0	34.0	41.0
26	37.72892215188149	40.0	35.0	41.0	34.0	41.0
27	37.70457315688608	40.0	35.0	41.0	34.0	41.0
28	37.61381892660242	39.0	35.0	41.0	34.0	41.0
29	37.565719488823696	39.0	35.0	41.0	34.0	41.0
30	37.45077657381084	40.0	35.0	41.0	34.0	41.0
31	37.31169583738272	39.0	35.0	41.0	33.0	41.0
32	37.04560668125959	39.0	35.0	41.0	33.0	41.0
33	36.641888663838905	39.0	35.0	41.0	32.0	41.0
34	36.342290574846274	39.0	35.0	41.0	31.0	41.0
35	36.126584710124526	39.0	35.0	41.0	30.0	41.0
36	36.04827110146323	39.0	35.0	41.0	30.0	41.0
37	35.91347741578034	39.0	35.0	41.0	30.0	41.0
38	35.76814321655964	38.0	35.0	41.0	30.0	41.0
39	35.685528478914435	38.0	35.0	41.0	29.0	41.0
40	35.60101227077448	38.0	35.0	41.0	29.0	41.0
41	35.51536344562621	38.0	35.0	40.0	29.0	41.0
42	35.4065277613784	38.0	35.0	40.0	29.0	41.0
43	35.28818199983651	37.0	35.0	40.0	28.0	41.0
44	35.17960154043179	37.0	35.0	40.0	27.0	41.0
45	35.11202326996612	37.0	35.0	40.0	27.0	41.0
46	35.01447379176923	37.0	35.0	40.0	26.0	41.0
47	34.87130309993733	36.0	35.0	40.0	26.0	41.0
48	34.80486153371057	36.0	35.0	40.0	26.0	41.0
49	34.745409585918125	36.0	35.0	40.0	26.0	41.0
50	34.680967583720104	36.0	35.0	40.0	26.0	41.0
51	34.2597816510595	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	4.0
14	12.0
15	21.0
16	68.0
17	147.0
18	349.0
19	691.0
20	1361.0
21	2254.0
22	3560.0
23	5691.0
24	9686.0
25	17818.0
26	29259.0
27	37445.0
28	39456.0
29	37761.0
30	37369.0
31	40969.0
32	47682.0
33	61751.0
34	140210.0
35	210930.0
36	206095.0
37	212153.0
38	331732.0
39	727318.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.929100173480233	16.585527570640966	23.623012016457913	30.862360239420884
2	36.07966466543747	19.428832232808656	23.433455344735194	21.058047757018684
3	25.275479341320086	20.78052480040691	34.428741405462354	19.51525445281065
4	22.697572185033472	23.504209847500885	32.624138275552006	21.17407969191364
5	21.48280184197858	30.68483819108257	29.248312882042526	18.584047084896323
6	77.02258876102417	2.006421493383228	18.019873023369875	2.951116722222727
7	76.86436752377406	1.8768108702168047	16.6742204743004	4.584601131708735
8	68.19807627680542	3.257068638225597	21.698108066376626	6.8467470185923585
9	41.018855757091345	15.162944259257577	26.339521703194396	17.47867828045668
10	27.279357669006988	16.27784993505845	35.60422892124361	20.83856347469096
11	24.219475199593095	13.081408550486382	40.79296814684965	21.906148103070873
12	20.196323309021878	14.279466661822541	42.86614773976149	22.658062289394092
13	16.230301819271748	17.268049664392958	44.456534573429366	22.04511394290593
14	16.529986648380092	17.371411184479424	43.96402328813159	22.13457887900889
15	20.227522502475047	14.404581331347242	43.1530259130419	22.214870253135814
16	19.622203653075868	18.26969363936094	38.9903178048847	23.117784902678498
17	20.205814766709963	19.43396397787446	38.343536271900746	22.016684983514835
18	20.966766273989773	24.584691959055032	34.83396761096831	19.614574155986887
19	21.717999255215762	20.88751941434527	34.00298821969319	23.391493110745785
20	21.622312645891427	24.452447342845986	34.4341002188939	19.491139792368685
21	19.350266578261383	19.591276941661594	35.63293036267359	25.42552611740343
22	20.720033787772824	21.238794176150556	33.924558806165365	24.116613229911263
23	21.408777554746184	19.49663484681968	36.66173171418451	22.43285588424963
24	23.519060118620512	17.874049718889363	34.120791287840945	24.48609887464918
25	18.511339794185233	18.988274189593003	36.7943850534519	25.70600096276987
26	18.237495345098502	18.47755202136259	36.776083343172964	26.508869290365944
27	21.57458287541213	20.78206886529396	35.85091599378741	21.7924322655065
28	22.225769534691505	21.448196623039266	32.067139574383056	24.258894267886173
29	21.591976312228088	18.67010599551313	36.04306124487961	23.694856447379177
30	24.374789961761685	20.971216813958346	31.26227304516844	23.391720179111527
31	25.590196096240657	18.93514019200901	31.226714139092998	24.247949572657333
32	23.29712349794276	19.845548097621233	31.233798672104196	25.62352973233181
33	18.815838472647346	23.580731886756464	34.1752876956194	23.428141944976794
34	21.181663775329476	22.918736773267696	31.21754057711696	24.682058874285868
35	21.174670069664575	25.14491503101754	30.21930262763513	23.461112271682758
36	23.348804257985993	25.969491094378693	27.626772268594628	23.054932379040682
37	23.339721523356253	25.56576354008665	30.351093107112693	20.743421829444408
38	22.226723221827626	22.959245769716347	29.667798980917176	25.14623202753885
39	22.80007084533011	21.62149519977475	34.23632367233127	21.342110282563876
40	19.056576354008666	22.043887773730912	35.3950081290475	23.50452774321293
41	20.429250038601623	23.80966221309912	31.389794639370024	24.371293108929237
42	20.041508097257925	19.34854085868173	32.96646654374699	27.643484500313352
43	22.337623411656782	20.564264888872742	33.138266469268565	23.95984523020191
44	22.14525109219884	21.17162735356361	32.97214325289058	23.71097830134697
45	22.336306415135468	21.3359794366888	32.54616299875567	23.781551149420068
46	22.66828036585255	20.167894349630785	32.681359503719385	24.482465780797284
47	20.318304435099318	21.455371983396763	34.19903904667617	24.027284534827746
48	21.241246514500585	20.32616100055405	31.345152998664837	27.08743948628053
49	20.471530168303072	18.358659025059264	35.33156522765874	25.83824557897892
50	20.250728889454038	18.47528133770516	36.044014932015735	25.229974840825076
51	19.226060182199657	17.556789798272465	35.714311664956085	27.502838354571796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	3370.5
2	6174.0
3	72201.5
4	138229.0
5	79685.0
6	21141.0
7	19201.0
8	17261.0
9	16755.5
10	16250.0
11	15792.0
12	15334.0
13	14492.0
14	13650.0
15	12760.0
16	11870.0
17	10944.0
18	10018.0
19	9301.5
20	8585.0
21	8221.5
22	7858.0
23	7461.5
24	7065.0
25	7172.5
26	8027.5
27	8775.0
28	9630.5
29	10486.0
30	11365.5
31	12245.0
32	14298.5
33	16352.0
34	19714.5
35	23077.0
36	25962.5
37	28848.0
38	30930.5
39	33013.0
40	39988.5
41	46964.0
42	59082.5
43	71201.0
44	96572.0
45	121943.0
46	155183.0
47	188423.0
48	206882.5
49	225342.0
50	201714.5
51	178087.0
52	152465.5
53	126844.0
54	119248.5
55	111653.0
56	109610.5
57	107568.0
58	108663.5
59	109759.0
60	109026.0
61	108293.0
62	105522.0
63	102751.0
64	98468.0
65	94185.0
66	78177.5
67	62170.0
68	51258.0
69	40346.0
70	35076.5
71	29807.0
72	27223.5
73	24640.0
74	21083.0
75	13894.5
76	10263.0
77	7717.5
78	5172.0
79	3894.5
80	2617.0
81	1955.5
82	1294.0
83	906.5
84	519.0
85	392.0
86	265.0
87	201.0
88	137.0
89	114.0
90	91.0
91	61.0
92	31.0
93	18.5
94	6.0
95	4.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2201980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.048231253667865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33351641812557	21.592571748120285
2	10.030775165906887	5.827525533456238
3	3.6682015418690463	3.1966430001981925
4	1.8825601997256973	2.1874017612233287
5	1.1729271921957194	1.7035730161308296
6	0.8160513184664742	1.4222908448204823
7	0.6743106079173844	1.371127133290988
8	0.5557411681502478	1.2914638375689533
9	0.4844467185644385	1.2665088279846328
>10	5.9175115213013925	36.824083917277136
>50	0.3177202299416689	6.094499588101038
>100	0.134734605122696	6.745169912224642
>500	0.00583044616946588	1.123387820537652
>1k	0.004727388786053417	2.613151491274284
>5k	6.303185048071223E-4	1.3183396878536762
>10k+	3.1515925240356113E-4	5.4222618799376034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104927	4.765120482474863	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13530	0.6144469977020681	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	8708	0.39546226577898075	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	8120	0.3687590259675383	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	6388	0.2901025440739698	TruSeq Adapter, Index 15 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	5585	0.25363536453555435	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	4132	0.1876492974504764	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	3828	0.17384354081326805	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	3702	0.1681214179965304	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3367	0.152907837491712	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3184	0.14459713530549778	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	2691	0.12220819444318295	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	2631	0.11948337405426025	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	2364	0.10735792332355425	TruSeq Adapter, Index 15 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2207	0.10022797663920653	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29854948727963015	0.0
2	0.0	0.0	0.0	0.973033360884295	0.0
3	0.0	0.0	0.0	1.6641386388613884	0.0
4	0.0	0.0	0.0	2.143888682004378	0.0
5	0.0	0.0	0.0	4.436416316224489	0.0
6	0.0	0.0	0.0	5.368668198621241	0.0
7	0.0	0.0	0.0	7.78853577235034	0.0
8	0.0	0.0	0.0	10.499595818308977	0.0
9	0.0	0.0	0.0	13.18545127567008	0.0
10	0.0	0.0	0.0	14.614574155986885	0.0
11	0.0	0.0	0.0	15.750370121436163	0.0
12	0.0	0.0	0.0	16.55728026594247	0.0
13	0.0	0.0	0.0	16.985849099446863	0.0
14	0.0	0.0	0.0	17.25669624610578	0.0
15	0.0	0.0	0.0	17.92700206178076	0.0
16	0.0	0.0	0.0	18.681186931761413	0.0
17	0.0	0.0	0.0	19.597680269575562	0.0
18	4.541367314871161E-5	0.0	0.0	20.123933914022835	0.0
19	4.541367314871161E-5	0.0	0.0	20.72502929181918	0.0
20	4.541367314871161E-5	0.0	0.0	21.36309139955858	0.0
21	4.541367314871161E-5	0.0	0.0	22.023497034487143	0.0
22	9.082734629742322E-5	0.0	0.0	22.644529014795776	0.0
23	9.082734629742322E-5	0.0	0.0	23.183543901397833	0.0
24	1.8165469259484644E-4	0.0	0.0	23.638089355943286	0.0
25	1.8165469259484644E-4	0.0	0.0	24.079782740987657	0.0
26	1.8165469259484644E-4	0.0	0.0	24.53818835775075	0.0
27	1.8165469259484644E-4	0.0	0.0	24.996548560840697	0.0
28	1.8165469259484644E-4	0.0	0.0	25.447097612149065	0.0
29	1.8165469259484644E-4	0.0	0.0	25.924758626327215	0.0
30	1.8165469259484644E-4	0.0	0.0	26.37053924195497	0.0
31	1.8165469259484644E-4	0.0	0.0	26.804239820525165	0.0
32	1.8165469259484644E-4	0.0	0.0	27.289757400158038	0.0
33	1.8165469259484644E-4	0.0	0.0	27.762195841924086	0.0
34	1.8165469259484644E-4	0.0	0.0	28.272690941788753	0.0
35	1.8165469259484644E-4	0.0	0.0	28.727418051026802	0.0
36	1.8165469259484644E-4	0.0	0.0	29.181781850879663	0.0
37	2.2706836574355808E-4	0.0	0.0	29.756582711922906	0.0
38	2.2706836574355808E-4	0.0	0.0	30.39223789498542	0.0
39	2.2706836574355808E-4	0.0	0.0	31.029164660896104	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.0346956E-4	45.0	40
TGCGATA	20	7.0346956E-4	45.0	37
CGACTTA	20	7.0346956E-4	45.0	15
CGTTTTT	26510	0.0	44.20219	1
ATAACGG	85	0.0	42.352943	1
TCGGGTA	80	0.0	42.1875	4
TATACGG	70	0.0	41.785713	1
CACTTAC	3290	0.0	41.2386	36
TGCATGG	470	0.0	41.17021	1
GCATAGG	470	0.0	41.17021	1
CGCACTT	3305	0.0	41.051437	34
AGGGTAC	615	0.0	40.975613	5
TATGCGG	440	0.0	40.909092	1
ACTACGG	110	0.0	40.909092	1
GCACTTA	3335	0.0	40.68216	35
ACTTACT	3350	0.0	40.56716	37
CCGCACT	3340	0.0	40.55389	33
CTTACTG	3330	0.0	40.472973	38
CCCGCAC	3435	0.0	39.956333	32
ACCCGCA	3405	0.0	39.911896	31
>>END_MODULE