FastQCFastQC Report
Thu 26 May 2016
SRR1043216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043216_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences834565
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461285.527190811979894No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT66550.797421411154314Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT54060.6477626068670506Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT42140.5049337079796061Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC29810.3571920701203621Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG19740.2365304080568919No Hit
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT19280.2310185545763362Illumina Single End Adapter 1 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT16710.20022406882627478No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC15170.18177134195658817Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC13760.1648763128096673Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC11790.1412712011646786Illumina Single End Adapter 1 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC11370.13623863929112773Illumina Single End Adapter 1 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG10670.12785103616854288Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC10360.12413652621425532No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT10210.12233918268798716No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT9890.11850484983194837No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG9280.11119565282512447Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC9190.11011724670936356Illumina Single End Adapter 1 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC8880.106402736755076Illumina Single End Adapter 1 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT8580.10280804970253965Illumina Single End Adapter 1 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC8350.10005212296226176Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAATT206.310455E-446.0000048
GTCGATG206.310455E-446.00000446
AGTTTCG206.310455E-446.00000410
TAGGTGA206.310455E-446.00000411
CGGCACA206.310455E-446.00000413
ACGGGCC206.310455E-446.00000417
AATGCGG405.6079443E-946.0000041
CTACGGT206.310455E-446.0000042
TTGCGAC206.310455E-446.00000415
TTAACTA206.310455E-446.00000433
GCGATAC1050.046.0000048
GCGGCAC206.310455E-446.00000412
CGTCGAT206.310455E-446.00000445
CACCGAA206.310455E-446.00000423
ATGCCGC206.310455E-446.00000446
AGTACCG206.310455E-446.00000439
AATCGAC206.310455E-446.0000041
TGCGTCG206.310455E-446.00000443
GCGAGAC301.8607861E-646.020
TCACGAC600.046.024
Produced by FastQC (version 0.11.5)