##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043216_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	834565
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22523350487979	33.0	31.0	34.0	30.0	34.0
2	32.55091454829762	34.0	31.0	34.0	31.0	34.0
3	32.62863048414444	34.0	31.0	34.0	31.0	34.0
4	36.14095726516209	37.0	35.0	37.0	35.0	37.0
5	36.02443188966707	37.0	35.0	37.0	35.0	37.0
6	36.28084331358252	37.0	37.0	37.0	35.0	37.0
7	36.20679875144536	37.0	37.0	37.0	35.0	37.0
8	36.18394253293632	37.0	37.0	37.0	35.0	37.0
9	37.747990869494885	39.0	38.0	39.0	35.0	39.0
10	37.55693205442356	39.0	37.0	39.0	35.0	39.0
11	37.20707793880644	39.0	37.0	39.0	34.0	39.0
12	37.1997399843032	39.0	37.0	39.0	34.0	39.0
13	37.19792466734167	39.0	37.0	39.0	34.0	39.0
14	38.58455602619328	40.0	38.0	41.0	34.0	41.0
15	38.68668947295897	40.0	38.0	41.0	35.0	41.0
16	38.70571016038296	40.0	38.0	41.0	35.0	41.0
17	38.67166487930838	40.0	38.0	41.0	35.0	41.0
18	38.418607298412944	40.0	38.0	41.0	34.0	41.0
19	38.133467135573625	40.0	37.0	41.0	34.0	41.0
20	37.843918688178874	40.0	35.0	41.0	34.0	41.0
21	37.77396967282357	40.0	35.0	41.0	34.0	41.0
22	37.72409458819864	40.0	35.0	41.0	34.0	41.0
23	37.736413580727685	39.0	35.0	41.0	34.0	41.0
24	37.6487583351806	39.0	35.0	41.0	34.0	41.0
25	37.557922989821044	39.0	35.0	41.0	34.0	41.0
26	37.497114065411324	39.0	35.0	41.0	34.0	41.0
27	37.49488296298072	39.0	35.0	41.0	34.0	41.0
28	37.45436125406649	39.0	35.0	41.0	33.0	41.0
29	37.39756160394936	39.0	35.0	41.0	33.0	41.0
30	37.18488194448605	39.0	35.0	41.0	33.0	41.0
31	37.1145327206389	39.0	35.0	41.0	33.0	41.0
32	36.714312246499674	39.0	35.0	41.0	32.0	41.0
33	36.47451426791203	39.0	35.0	41.0	31.0	41.0
34	36.12852204441835	39.0	35.0	41.0	30.0	41.0
35	36.02676244510614	39.0	35.0	41.0	30.0	41.0
36	35.95914997633498	39.0	35.0	41.0	30.0	41.0
37	35.966278240760154	39.0	35.0	41.0	30.0	41.0
38	35.946257032106544	39.0	35.0	41.0	30.0	41.0
39	35.96438144422544	39.0	35.0	41.0	29.0	41.0
40	35.92744004361553	39.0	35.0	41.0	30.0	41.0
41	35.92975262561934	39.0	35.0	41.0	30.0	41.0
42	35.83723017380312	39.0	35.0	41.0	29.0	41.0
43	35.730301414509356	39.0	35.0	41.0	29.0	41.0
44	35.57476050397513	38.0	35.0	41.0	28.0	41.0
45	35.514287083690306	38.0	35.0	41.0	27.0	41.0
46	35.48294860196629	38.0	35.0	41.0	27.0	41.0
47	35.37582213488464	38.0	35.0	40.0	26.0	41.0
48	35.34014007297215	38.0	35.0	40.0	26.0	41.0
49	35.27609113729907	38.0	35.0	40.0	26.0	41.0
50	35.12642154895065	38.0	35.0	40.0	26.0	41.0
51	35.03886455818301	38.0	35.0	40.0	26.0	41.0
52	34.70303930790292	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	2.0
14	4.0
15	14.0
16	32.0
17	63.0
18	174.0
19	331.0
20	637.0
21	1026.0
22	1665.0
23	2848.0
24	5388.0
25	9705.0
26	13573.0
27	14479.0
28	12905.0
29	12465.0
30	13313.0
31	15962.0
32	19489.0
33	25700.0
34	46452.0
35	83010.0
36	77632.0
37	64708.0
38	107737.0
39	304974.0
40	275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.7193088615027	19.014935924703288	25.51077507443998	28.754980139354036
2	33.35617956660057	19.873706661554223	26.76663890769443	20.003474864150785
3	20.93737456039973	21.48999778327632	38.748090322503344	18.82453733382061
4	22.38926866091916	24.132332412694037	36.32359372846932	17.154805197917476
5	20.040140672086658	29.308681768346386	31.724431290552563	18.926746269014398
6	72.54473887594136	1.630070755423484	21.739109596017087	4.086080772618071
7	73.23156374877931	1.3720920479531253	18.07169004211775	7.324654161149821
8	60.85877073685093	3.084600959781443	26.43041584538053	9.626212457987094
9	34.98481244720303	17.37036659816791	29.286874000227662	18.357946954401395
10	26.95763661308586	19.34936164349092	42.252311084217524	11.440690659205693
11	23.981595202291015	15.614721441709154	40.84175588480226	19.56192747119757
12	19.000197707787887	19.56696003307112	43.39302510888906	18.039817150251928
13	13.375710699586014	23.02193358216556	42.485246805221884	21.11710891302655
14	12.11673147088603	21.803214848453987	45.3442212410058	20.735832439654192
15	12.990000778848861	18.21128372265791	48.261789075746044	20.53692642274718
16	13.403509612792295	24.479339536165547	39.083714270308484	23.033436580733678
17	15.30378101166476	21.619286694265874	41.680875665765996	21.396056628303366
18	17.324833895502447	28.21865283111561	39.02416228813813	15.43235098524381
19	16.857284932869216	23.176864594129874	36.334857081233935	23.630993391766967
20	14.180321484845399	29.9877181525705	35.10307765123148	20.728882711352618
21	14.750798320082916	23.65555708662597	36.78251544217646	24.811129151114653
22	12.747239579900906	26.88658163234739	32.80798979108877	27.55818899666293
23	12.412094923702767	23.13756268235548	42.70080820547231	21.74953418846944
24	17.21938974196138	21.520672446124628	39.16974711376585	22.090190698148138
25	13.584681840240126	25.039631424754212	38.83687909270099	22.538807642304672
26	16.751720956426404	21.75109188619221	37.33597742536531	24.161209732016083
27	21.914170855475607	21.472024348013637	39.435753955653546	17.17805084085721
28	17.20549028535824	21.993014324827904	35.99192393642197	24.80957145339189
29	23.72685171316794	21.24184455374956	34.029464451540626	21.00183928154188
30	23.04661709992631	20.348684644096025	35.142379563005875	21.462318692971788
31	28.764086679887125	20.243839605063716	30.45251118846345	20.539562526585705
32	23.086637949111214	20.86320418421573	36.82337505167363	19.226782814999428
33	27.137970080221436	19.77053914314643	34.90800596718051	18.18348480945163
34	22.017697842588653	22.75976107313391	37.355987849957764	17.866553234319678
35	30.54753074955216	18.637853252892224	35.0610198127168	15.753596184838809
36	24.078891398512997	27.22951477715936	33.141097457957144	15.550496366370503
37	24.693942353201965	25.03759443542444	35.10128030770521	15.167182903668378
38	22.32468411687526	27.27912145848436	30.591385931593106	19.804808493047275
39	26.69019189637715	24.830300815394846	32.37375159514238	16.10575569308562
40	19.202458765943938	22.51939633222098	37.43483131931006	20.843313582525028
41	23.143913296148295	23.862970529557316	30.960081000281587	22.03303517401281
42	22.251232678101765	18.580817551658647	37.54842343017021	21.619526340069378
43	26.25679246074302	21.072175324869843	34.3038588965509	18.36717331783624
44	18.762349247811734	23.68922732201806	36.01073613199691	21.5376872981733
45	23.257864875713697	21.098895831960363	33.96595831361248	21.677280978713462
46	25.812129672344277	20.017494143655675	33.2158669486499	20.95450923535015
47	20.31561352321269	17.95282572358055	38.1010466530468	23.630514100159964
48	20.757041093264156	16.755794935085945	32.70434298107398	29.78282099057593
49	22.552587276006065	16.459472899055196	38.350757580296325	22.637182244642418
50	21.025803861892122	16.449647421111596	38.4124663746982	24.112082342298084
51	18.35063775739457	15.953101316254575	37.70059851539425	27.995662410956605
52	16.962729086410285	15.906849676178608	45.31019153690845	21.820229700502658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	224.0
1	1095.5
2	1967.0
3	26962.0
4	51957.0
5	28958.5
6	5960.0
7	5915.0
8	5870.0
9	6193.0
10	6516.0
11	6626.5
12	6737.0
13	6741.0
14	6255.5
15	5766.0
16	5252.5
17	4739.0
18	4344.0
19	3949.0
20	3646.5
21	3344.0
22	3056.0
23	2768.0
24	2825.5
25	2883.0
26	2921.5
27	2960.0
28	3118.0
29	3276.0
30	3541.0
31	3806.0
32	4963.5
33	6121.0
34	6897.5
35	7674.0
36	8990.5
37	10307.0
38	11441.0
39	14617.5
40	16660.0
41	23231.5
42	29803.0
43	40078.5
44	50354.0
45	65464.5
46	80575.0
47	102409.5
48	124244.0
49	128846.0
50	133448.0
51	115905.0
52	98362.0
53	78364.0
54	58366.0
55	45493.5
56	32621.0
57	26107.0
58	19593.0
59	16163.5
60	12734.0
61	10334.5
62	7935.0
63	6263.0
64	3682.0
65	2773.0
66	2372.0
67	1971.0
68	1627.0
69	1283.0
70	1096.0
71	909.0
72	882.0
73	855.0
74	691.5
75	528.0
76	442.0
77	356.0
78	274.0
79	192.0
80	144.5
81	97.0
82	98.0
83	99.0
84	76.0
85	53.0
86	31.0
87	9.0
88	5.5
89	3.5
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	834565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.997948198575266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3105970156448	24.19099282728874
2	11.884562348650238	7.843323454869972
3	4.821889351825162	4.773373651523652
4	2.4432563966278154	3.224897920470492
5	1.4470587130990986	2.3874984227570524
6	0.8942535909710494	1.770512020275156
7	0.6735140735952109	1.5557207758054326
8	0.4899246655867816	1.2933206988989543
9	0.3680623972680304	1.093077352700451
>10	3.109757845710779	21.90050483881226
>50	0.36776725256646686	8.230754118131149
>100	0.1754921071419288	10.308487795122453
>500	0.008391514478720075	1.881614450971324
>1k	0.004378181467158299	2.540402234944392
>5k	7.296969111930499E-4	1.4520539953397618
>10k+	3.6484845559652494E-4	5.5534654420887595
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46128	5.527190811979894	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	6655	0.797421411154314	Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT	5406	0.6477626068670506	Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	4214	0.5049337079796061	Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2981	0.3571920701203621	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	1974	0.2365304080568919	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	1928	0.2310185545763362	Illumina Single End Adapter 1 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	1671	0.20022406882627478	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1517	0.18177134195658817	Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1376	0.1648763128096673	Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1179	0.1412712011646786	Illumina Single End Adapter 1 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1137	0.13623863929112773	Illumina Single End Adapter 1 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1067	0.12785103616854288	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	1036	0.12413652621425532	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT	1021	0.12233918268798716	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	989	0.11850484983194837	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	928	0.11119565282512447	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	919	0.11011724670936356	Illumina Single End Adapter 1 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	888	0.106402736755076	Illumina Single End Adapter 1 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	858	0.10280804970253965	Illumina Single End Adapter 1 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	835	0.10005212296226176	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6662153337367371	0.0
2	0.0	0.0	0.0	2.346491884993979	0.0
3	0.0	0.0	0.0	3.918208887264623	0.0
4	0.0	0.0	0.0	4.971691839461276	0.0
5	0.0	0.0	0.0	10.14684296609611	0.0
6	0.0	0.0	0.0	11.559075686135891	0.0
7	0.0	0.0	0.0	16.095331100633263	0.0
8	0.0	0.0	0.0	21.45297250663519	0.0
9	0.0	0.0	0.0	25.795474289000857	0.0
10	0.0	0.0	0.0	28.2869518851138	0.0
11	0.0	0.0	0.0	30.438252263155057	0.0
12	0.0	0.0	0.0	31.986723622485965	0.0
13	0.0	0.0	0.0	32.63532498966527	0.0
14	1.1982290175121171E-4	0.0	0.0	33.104192004217765	0.0
15	1.1982290175121171E-4	0.0	0.0	34.582447143122465	0.0
16	1.1982290175121171E-4	0.0	0.0	36.2868080976317	0.0
17	2.3964580350242342E-4	0.0	0.0	38.21895238836999	0.0
18	2.3964580350242342E-4	0.0	0.0	39.27567055891392	0.0
19	2.3964580350242342E-4	0.0	0.0	40.391221774217705	0.0
20	2.3964580350242342E-4	0.0	0.0	41.667695146573365	0.0
21	2.3964580350242342E-4	0.0	0.0	42.848669666233306	0.0
22	0.0010784061157609055	0.0	0.0	44.011311281925316	0.0
23	0.0010784061157609055	0.0	0.0	44.91573454434346	0.0
24	0.002156812231521811	0.0	0.0	45.662950159664014	0.0
25	0.002156812231521811	0.0	0.0	46.40034029704098	0.0
26	0.002156812231521811	0.0	0.0	47.15606333838586	0.0
27	0.002156812231521811	0.0	0.0	47.838095295153764	0.0
28	0.002156812231521811	0.0	0.0	48.56338331945385	0.0
29	0.002156812231521811	0.0	0.0	49.365837292481714	0.0
30	0.002156812231521811	0.0	0.0	50.08058090142769	0.0
31	0.002156812231521811	0.0	0.0	50.726785810571975	0.0
32	0.002156812231521811	0.0	0.0	51.37886204190207	0.0
33	0.002156812231521811	0.0	0.0	52.01763793113778	0.0
34	0.002156812231521811	0.0	0.0	52.69931041920042	0.0
35	0.002156812231521811	0.0	0.0	53.27158459796421	0.0
36	0.0022766351332730223	0.0	0.0	53.83882621485444	0.0
37	0.0022766351332730223	0.0	0.0	54.42356197540036	0.0
38	0.0022766351332730223	0.0	0.0	54.984213332694274	0.0
39	0.0022766351332730223	0.0	0.0	55.53048594177805	0.0
40	0.0022766351332730223	0.0	0.0	56.08526597688616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAATT	20	6.310455E-4	46.000004	8
GTCGATG	20	6.310455E-4	46.000004	46
AGTTTCG	20	6.310455E-4	46.000004	10
TAGGTGA	20	6.310455E-4	46.000004	11
CGGCACA	20	6.310455E-4	46.000004	13
ACGGGCC	20	6.310455E-4	46.000004	17
AATGCGG	40	5.6079443E-9	46.000004	1
CTACGGT	20	6.310455E-4	46.000004	2
TTGCGAC	20	6.310455E-4	46.000004	15
TTAACTA	20	6.310455E-4	46.000004	33
GCGATAC	105	0.0	46.000004	8
GCGGCAC	20	6.310455E-4	46.000004	12
CGTCGAT	20	6.310455E-4	46.000004	45
CACCGAA	20	6.310455E-4	46.000004	23
ATGCCGC	20	6.310455E-4	46.000004	46
AGTACCG	20	6.310455E-4	46.000004	39
AATCGAC	20	6.310455E-4	46.000004	1
TGCGTCG	20	6.310455E-4	46.000004	43
GCGAGAC	30	1.8607861E-6	46.0	20
TCACGAC	60	0.0	46.0	24
>>END_MODULE