SRR1043215_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043215_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	821530
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42555	5.179969082078561	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	6607	0.8042311297213736	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	5350	0.6512239358270544	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	4215	0.5130670821515952	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	2993	0.3643202317626867	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1935	0.23553613379913088	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	1907	0.2321278589948024	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	1600	0.19475856024734337	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1581	0.19244580234440617	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1423	0.17321339451998102	Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1140	0.13876547417623217	Illumina Single End Adapter 1 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	1054	0.12829720156293745	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1040	0.1265930641607732	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	1005	0.12233272065536255	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	983	0.11965479045196158	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT	972	0.1183158253502611	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	940	0.11442065414531423	No Hit
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	898	0.10930824193882147	Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	864	0.10516962253356542	Illumina Single End Adapter 1 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	861	0.10480445023310166	Illumina Single End Adapter 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	842	0.10249169233016445	Illumina Single End Adapter 1 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	65	0.0	45.000004	24
CTGTCGG	20	7.0321094E-4	45.000004	1
TCGCAGT	20	7.0321094E-4	45.000004	40
ATTAGTA	20	7.0321094E-4	45.000004	21
GCGACGG	20	7.0321094E-4	45.000004	1
CGACGTT	40	6.8102963E-9	45.000004	26
CGACGGC	20	7.0321094E-4	45.000004	2
ACGTTGT	20	7.0321094E-4	45.000004	36
GACGTTC	20	7.0321094E-4	45.000004	11
GGCCGAA	20	7.0321094E-4	45.000004	10
CAACCCG	40	6.8102963E-9	45.000004	22
GCGCCTA	20	7.0321094E-4	45.000004	28
CACGACG	65	0.0	45.000004	25
CATTACG	20	7.0321094E-4	45.000004	32
GTTACCA	20	7.0321094E-4	45.000004	17
ACGGCAT	20	7.0321094E-4	45.000004	4
CGTAGCT	20	7.0321094E-4	45.000004	2
GCTTAGC	20	7.0321094E-4	45.000004	11
TTAACGG	40	6.8102963E-9	45.000004	1
TCCCGGC	20	7.0321094E-4	45.000004	39

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content