##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043215_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	821530
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14822465399924	33.0	31.0	34.0	30.0	34.0
2	32.43965162562536	34.0	31.0	34.0	31.0	34.0
3	32.51489781261792	34.0	31.0	34.0	31.0	34.0
4	36.081328740277286	37.0	35.0	37.0	35.0	37.0
5	36.02428639246284	37.0	35.0	37.0	35.0	37.0
6	36.278253989507384	37.0	37.0	37.0	35.0	37.0
7	36.2096843694083	37.0	37.0	37.0	35.0	37.0
8	36.11466166786362	37.0	37.0	37.0	35.0	37.0
9	37.68579358027096	39.0	38.0	39.0	35.0	39.0
10	37.4945674534101	39.0	37.0	39.0	35.0	39.0
11	37.19735615254464	39.0	37.0	39.0	34.0	39.0
12	37.19823500054776	39.0	37.0	39.0	34.0	39.0
13	37.19537813591713	39.0	37.0	39.0	34.0	39.0
14	38.580171144084815	40.0	38.0	41.0	34.0	41.0
15	38.69965308631456	40.0	38.0	41.0	35.0	41.0
16	38.7510255255438	40.0	38.0	41.0	35.0	41.0
17	38.689603544605795	40.0	38.0	41.0	35.0	41.0
18	38.46570788650445	40.0	38.0	41.0	35.0	41.0
19	38.206369822161086	40.0	37.0	41.0	34.0	41.0
20	37.94733241634511	40.0	35.0	41.0	34.0	41.0
21	37.869209888865896	40.0	35.0	41.0	34.0	41.0
22	37.846352537338866	40.0	35.0	41.0	34.0	41.0
23	37.826227891860306	40.0	35.0	41.0	34.0	41.0
24	37.74103806312612	40.0	35.0	41.0	34.0	41.0
25	37.72129806580405	40.0	35.0	41.0	34.0	41.0
26	37.694931408469564	40.0	35.0	41.0	34.0	41.0
27	37.65610263776125	39.0	35.0	41.0	34.0	41.0
28	37.58155392986257	39.0	35.0	41.0	34.0	41.0
29	37.553201952454565	39.0	35.0	41.0	34.0	41.0
30	37.29845166944603	39.0	35.0	41.0	33.0	41.0
31	37.21475417817974	39.0	35.0	41.0	33.0	41.0
32	36.84867746765182	39.0	35.0	41.0	32.0	41.0
33	36.58067751634146	39.0	35.0	41.0	31.0	41.0
34	36.19320170900637	39.0	35.0	41.0	30.0	41.0
35	36.09373607780604	39.0	35.0	41.0	30.0	41.0
36	35.99658198726766	39.0	35.0	41.0	30.0	41.0
37	35.94918018818546	39.0	35.0	41.0	30.0	41.0
38	35.90801066303117	39.0	35.0	41.0	29.0	41.0
39	35.87880418244008	39.0	35.0	41.0	29.0	41.0
40	35.80874952831911	39.0	35.0	41.0	29.0	41.0
41	35.77318296349494	39.0	35.0	41.0	29.0	41.0
42	35.667325599795504	39.0	35.0	41.0	28.0	41.0
43	35.55096344625272	38.0	35.0	40.0	27.0	41.0
44	35.43563229583825	38.0	35.0	40.0	27.0	41.0
45	35.40305649215488	38.0	35.0	40.0	26.0	41.0
46	35.316517960390975	38.0	35.0	40.0	26.0	41.0
47	35.1675605273088	38.0	35.0	40.0	25.0	41.0
48	35.15090014972064	38.0	35.0	40.0	25.0	41.0
49	35.082930629435324	38.0	35.0	40.0	26.0	41.0
50	34.97083977456697	38.0	35.0	40.0	25.0	41.0
51	34.53199761420764	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	11.0
16	27.0
17	79.0
18	144.0
19	313.0
20	543.0
21	947.0
22	1469.0
23	2446.0
24	4447.0
25	8127.0
26	12788.0
27	14518.0
28	13750.0
29	13081.0
30	13468.0
31	15797.0
32	19294.0
33	25446.0
34	46324.0
35	81153.0
36	73330.0
37	64801.0
38	114472.0
39	294726.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.023724027120128	19.13003785619515	25.626574805545747	28.21966331113897
2	33.07608973500663	19.817413849768116	26.862804766715765	20.243691648509486
3	21.16903825788468	21.519116769929276	38.392511533358494	18.919333438827554
4	22.531130938614535	24.407873114797997	35.86564093824936	17.1953550083381
5	20.270349226443344	29.48875877935072	31.227222377758572	19.01366961644736
6	73.04809319197108	1.5726753739972978	21.233186858666148	4.146044575365477
7	73.65281852153907	1.3564933721227466	17.575742821321192	7.41494528501698
8	61.14712791985685	3.0126714788260927	26.028994680656826	9.811205920660232
9	35.1174028945991	17.426752522731974	28.83656105072243	18.61928353194649
10	27.137170888464208	19.36910398889876	41.94539456867065	11.54833055396638
11	24.192786629824838	15.642277214465691	40.58671016274512	19.578225992964345
12	19.212931968400422	19.408055700948232	43.10469489854296	18.274317432108383
13	13.471206164108432	22.91151875159763	42.29413411561355	21.32314096868039
14	12.248974474456197	21.74783635411975	45.075894976446385	20.927294194977662
15	13.090574902925031	18.124353340717928	47.86751548939174	20.917556266965295
16	13.470719267707814	24.382311053765534	38.99370686402201	23.153262814504643
17	15.517753460007548	21.511813323920002	41.50329263690919	21.467140579163267
18	17.481163195501075	28.29501052913466	38.82256277920465	15.401263496159604
19	16.943507845118255	23.142672817791194	36.16398670772826	23.749832629362288
20	14.211166968948183	30.121115479653817	34.731294048908744	20.93642350248926
21	14.786191618078465	23.604372329677553	36.52100349348167	25.088432558762307
22	12.860638077733011	26.955558531033557	32.41391063016567	27.769892761067766
23	12.54817231263618	23.110537655350385	42.24106240794615	22.10022762406729
24	17.323165313500418	21.62732949496671	38.71337626136599	22.336128930166886
25	13.617640256594404	24.92215743795114	38.7229924652782	22.737209840176256
26	16.87558579723199	21.563789514686015	37.06352780787068	24.497096880211313
27	22.06176280841844	21.336287171497084	39.23216437622485	17.36978564385963
28	17.45499251396784	22.03705281608706	35.568877582072474	24.939077087872626
29	23.89955327255243	21.1762199797938	33.68933575158546	21.234890996068312
30	23.271091743454285	20.194880284347498	34.66069407081908	21.873333901379134
31	29.17909266855745	20.02544033693231	30.024709992331385	20.77075700217886
32	23.373705159884604	20.662909449441898	36.46513213151072	19.49825325916278
33	27.584141784231857	19.48206395384222	34.530814455954136	18.402979805971782
34	22.38822684503305	22.44531544800555	37.29796842476842	17.868489282192982
35	30.88067386461846	18.38532981144937	34.98168052292673	15.75231580100544
36	24.449137584750407	27.02725402602461	32.98857010699548	15.535038282229499
37	25.164631845459084	24.792642995386657	34.94601536158144	15.09670979757282
38	22.644821248158923	27.250982922108747	30.21837303567733	19.885822794054995
39	27.0878726279016	24.747483354229306	32.103635898871616	16.06100811899748
40	19.448711550399864	22.45809647852178	37.245870509902254	20.8473214611761
41	23.45343444548586	23.689822647986077	30.714885640207907	22.14185726632016
42	22.45140165301328	18.350760167005465	37.37672391756844	21.821114262412816
43	26.744002044964883	20.866797317200835	34.01835599430331	18.370844643530972
44	18.891823792192618	23.484717539225592	36.01670054654121	21.606758122040585
45	23.4399230703687	20.993268657261453	33.796209511521184	21.77059876084866
46	26.082553284724842	19.86269521502562	33.10859006974791	20.946161430501625
47	20.661813932540504	17.729115187515976	37.8159044709262	23.79316640901732
48	20.92985040108091	16.46574075201149	32.37301133251373	30.231397514393876
49	22.80306257835989	16.065146738402735	38.16184436356554	22.969946319671834
50	21.37463026304578	16.111888792862096	38.28052536121626	24.232955582875853
51	18.669555585310334	15.699730989738658	37.429065280634916	28.201648144316096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	1037.5
2	1863.0
3	25035.0
4	48207.0
5	27299.5
6	6392.0
7	6182.5
8	5973.0
9	6159.5
10	6346.0
11	6395.5
12	6445.0
13	6273.5
14	6102.0
15	5681.5
16	5261.0
17	4739.5
18	4218.0
19	3846.5
20	3475.0
21	3224.5
22	2974.0
23	2732.5
24	2491.0
25	2487.5
26	2609.5
27	2735.0
28	2953.0
29	3171.0
30	3831.5
31	4492.0
32	5274.0
33	6056.0
34	7038.0
35	8020.0
36	9788.5
37	11557.0
38	12670.0
39	13783.0
40	17663.0
41	21543.0
42	28922.0
43	36301.0
44	45451.0
45	54601.0
46	73881.0
47	93161.0
48	115232.0
49	137303.0
50	136046.5
51	134790.0
52	107899.0
53	81008.0
54	62727.0
55	44446.0
56	34753.0
57	25060.0
58	20126.5
59	15193.0
60	12236.0
61	9279.0
62	7609.5
63	5940.0
64	4603.0
65	3266.0
66	2880.5
67	2495.0
68	1939.0
69	1383.0
70	1272.5
71	1162.0
72	962.5
73	763.0
74	727.5
75	552.0
76	412.0
77	297.5
78	183.0
79	145.0
80	107.0
81	111.0
82	115.0
83	70.5
84	26.0
85	28.0
86	30.0
87	18.0
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.60937732991853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79775959057592	23.738896112617855
2	12.029652454692826	7.84558952085718
3	4.952776930524757	4.845209152751925
4	2.485768977580312	3.2423751417968876
5	1.4555029213639903	2.373152198377855
6	0.9592445798379043	1.8768221073368028
7	0.6450364622777429	1.4723966172979475
8	0.4986842003953472	1.3009425007328468
9	0.3790898649669877	1.1125696003790728
>10	3.2305366372473103	22.425565484959748
>50	0.37177926496401614	8.224340221277998
>100	0.18099779765616297	10.588298012762355
>500	0.007902190750061164	1.8237999439521473
>1k	0.0041392427738415625	2.4410181178120207
>5k	7.525895952439205E-4	1.4672122673643155
>10k+	3.7629479762196023E-4	5.221812999723046
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42555	5.179969082078561	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	6607	0.8042311297213736	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	5350	0.6512239358270544	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	4215	0.5130670821515952	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	2993	0.3643202317626867	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1935	0.23553613379913088	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	1907	0.2321278589948024	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	1600	0.19475856024734337	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1581	0.19244580234440617	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1423	0.17321339451998102	Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1140	0.13876547417623217	Illumina Single End Adapter 1 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	1054	0.12829720156293745	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1040	0.1265930641607732	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	1005	0.12233272065536255	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	983	0.11965479045196158	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT	972	0.1183158253502611	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	940	0.11442065414531423	No Hit
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	898	0.10930824193882147	Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	864	0.10516962253356542	Illumina Single End Adapter 1 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	861	0.10480445023310166	Illumina Single End Adapter 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	842	0.10249169233016445	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6758122040582815	0.0
2	0.0	0.0	0.0	2.3904178788358306	0.0
3	0.0	0.0	0.0	3.988168417464974	0.0
4	0.0	0.0	0.0	5.053254293817633	0.0
5	0.0	0.0	0.0	10.247465095614281	0.0
6	0.0	0.0	0.0	11.666281207016176	0.0
7	0.0	0.0	0.0	16.283032877679453	0.0
8	0.0	0.0	0.0	21.770111864448044	0.0
9	0.0	0.0	0.0	26.342799410855356	0.0
10	0.0	0.0	0.0	28.90253551300622	0.0
11	0.0	0.0	0.0	31.101237934098574	0.0
12	0.0	0.0	0.0	32.69594537022385	0.0
13	0.0	0.0	0.0	33.37784377928986	0.0
14	0.0	0.0	0.0	33.85147225299137	0.0
15	0.0	0.0	0.0	35.37789246892992	0.0
16	0.0	0.0	0.0	37.135953647462664	0.0
17	0.0	0.0	0.0	39.11421372317505	0.0
18	0.0	0.0	0.0	40.186968217837446	0.0
19	0.0	0.0	0.0	41.32082821077745	0.0
20	0.0	0.0	0.0	42.5977140213991	0.0
21	0.0	0.0	0.0	43.81008605893881	0.0
22	8.520687010821272E-4	0.0	0.0	44.95672708239504	0.0
23	9.737928012367169E-4	0.0	0.0	45.8612588706438	0.0
24	0.00304310250386474	0.0	0.0	46.647596557642444	0.0
25	0.00304310250386474	0.0	0.0	47.394617360291164	0.0
26	0.00304310250386474	0.0	0.0	48.160992294864464	0.0
27	0.00304310250386474	0.0	0.0	48.87100897106618	0.0
28	0.0031648266040193297	0.0	0.0	49.58358185337115	0.0
29	0.0031648266040193297	0.0	0.0	50.39962022080752	0.0
30	0.0031648266040193297	0.0	0.0	51.15248378026365	0.0
31	0.0031648266040193297	0.0	0.0	51.816123574306474	0.0
32	0.0031648266040193297	0.0	0.0	52.48402371185471	0.0
33	0.0031648266040193297	0.0	0.0	53.14279454189135	0.0
34	0.0032865507041739193	0.0	0.0	53.83443087896973	0.0
35	0.0032865507041739193	0.0	0.0	54.418584835611604	0.0
36	0.0032865507041739193	0.0	0.0	55.00018258615023	0.0
37	0.0032865507041739193	0.0	0.0	55.58908378269814	0.0
38	0.0032865507041739193	0.0	0.0	56.143415334802135	0.0
39	0.0032865507041739193	0.0	0.0	56.6967730941049	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	65	0.0	45.000004	24
CTGTCGG	20	7.0321094E-4	45.000004	1
TCGCAGT	20	7.0321094E-4	45.000004	40
ATTAGTA	20	7.0321094E-4	45.000004	21
GCGACGG	20	7.0321094E-4	45.000004	1
CGACGTT	40	6.8102963E-9	45.000004	26
CGACGGC	20	7.0321094E-4	45.000004	2
ACGTTGT	20	7.0321094E-4	45.000004	36
GACGTTC	20	7.0321094E-4	45.000004	11
GGCCGAA	20	7.0321094E-4	45.000004	10
CAACCCG	40	6.8102963E-9	45.000004	22
GCGCCTA	20	7.0321094E-4	45.000004	28
CACGACG	65	0.0	45.000004	25
CATTACG	20	7.0321094E-4	45.000004	32
GTTACCA	20	7.0321094E-4	45.000004	17
ACGGCAT	20	7.0321094E-4	45.000004	4
CGTAGCT	20	7.0321094E-4	45.000004	2
GCTTAGC	20	7.0321094E-4	45.000004	11
TTAACGG	40	6.8102963E-9	45.000004	1
TCCCGGC	20	7.0321094E-4	45.000004	39
>>END_MODULE