FastQCFastQC Report
Thu 26 May 2016
SRR1043214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043214_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences905798
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400504.421515613856511No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT63610.7022537033643262No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT53270.5881002166045851Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT42480.4689787347730951No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC25700.2837277185421032No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT18920.20887659279441995No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG18900.20865579301345333No Hit
CGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG16910.1866862148072749No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC14130.1559950452529151No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC13910.15356624766228233No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT13100.1446238565331343No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC11180.12342707756033905No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT10440.11525748566457422No Hit
TCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC9580.10576309508300967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG700.046.0000041
GAATAGT351.0192525E-746.0000048
TTACCGG351.0192525E-746.0000041
AGCGTTG206.3107436E-446.030
AACGTGC206.3107436E-446.013
CGGCGAT405.6097633E-946.05
CTCACGC206.3107436E-446.046
ACCGGCC405.6097633E-946.020
TCCGAGG405.6097633E-946.01
ACGCACC206.3107436E-446.020
CGTGAGT206.3107436E-446.031
GCCGGTC405.6097633E-946.031
AGGGTCG501.6370905E-1146.05
CACGACC301.8609626E-646.026
ACGGGTC405.6097633E-946.04
CGTTCCG206.3107436E-446.018
AGTATAC253.4165787E-546.030
CGTTAGC206.3107436E-446.018
ACGATGG206.3107436E-446.01
GCCGATG301.8609626E-646.08
Produced by FastQC (version 0.11.5)