##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043214_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	905798
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26160578848706	33.0	31.0	34.0	31.0	34.0
2	32.594313522440984	34.0	31.0	34.0	31.0	34.0
3	32.68886661264432	34.0	31.0	34.0	31.0	34.0
4	36.177517503902635	37.0	35.0	37.0	35.0	37.0
5	36.06776345277866	37.0	35.0	37.0	35.0	37.0
6	36.317646980894196	37.0	37.0	37.0	35.0	37.0
7	36.247294650683706	37.0	37.0	37.0	35.0	37.0
8	36.22453571326057	37.0	37.0	37.0	35.0	37.0
9	37.754971859067915	39.0	38.0	39.0	35.0	39.0
10	37.53992391239548	39.0	37.0	39.0	35.0	39.0
11	37.29794280844074	39.0	37.0	39.0	35.0	39.0
12	37.31679248574186	39.0	37.0	39.0	34.0	39.0
13	37.323424207163185	39.0	37.0	39.0	34.0	39.0
14	38.70404438958796	40.0	38.0	41.0	35.0	41.0
15	38.7811233851256	40.0	38.0	41.0	35.0	41.0
16	38.797479128900704	40.0	38.0	41.0	35.0	41.0
17	38.79975778264028	40.0	38.0	41.0	35.0	41.0
18	38.54871174367795	40.0	38.0	41.0	35.0	41.0
19	38.30206514035138	40.0	37.0	41.0	35.0	41.0
20	38.021993866182086	40.0	35.0	41.0	34.0	41.0
21	37.96297960472423	40.0	35.0	41.0	34.0	41.0
22	37.90302584019837	40.0	35.0	41.0	34.0	41.0
23	37.91109828019051	40.0	35.0	41.0	34.0	41.0
24	37.8259413246662	40.0	35.0	41.0	34.0	41.0
25	37.741444560487	40.0	35.0	41.0	34.0	41.0
26	37.68994301157653	40.0	35.0	41.0	34.0	41.0
27	37.694077487475134	40.0	35.0	41.0	34.0	41.0
28	37.60737051748845	40.0	35.0	41.0	34.0	41.0
29	37.520563083601424	40.0	35.0	41.0	33.0	41.0
30	37.48078379506248	40.0	35.0	41.0	33.0	41.0
31	37.167736073605816	39.0	35.0	41.0	33.0	41.0
32	37.01017334990804	39.0	35.0	41.0	32.0	41.0
33	36.70989227178686	39.0	35.0	41.0	31.0	41.0
34	36.39233581880287	39.0	35.0	41.0	30.0	41.0
35	36.23870001920958	39.0	35.0	41.0	30.0	41.0
36	36.24986034413854	39.0	35.0	41.0	30.0	41.0
37	36.275759054447015	39.0	35.0	41.0	30.0	41.0
38	36.25116637484295	39.0	35.0	41.0	30.0	41.0
39	36.27714567707149	40.0	35.0	41.0	30.0	41.0
40	36.28262592763508	39.0	35.0	41.0	30.0	41.0
41	36.213414028293286	39.0	35.0	41.0	30.0	41.0
42	36.07458506201162	39.0	35.0	41.0	30.0	41.0
43	35.92563352977154	39.0	35.0	41.0	29.0	41.0
44	35.80849703797094	39.0	35.0	41.0	29.0	41.0
45	35.739844866073895	39.0	35.0	41.0	28.0	41.0
46	35.70135394425689	39.0	35.0	41.0	27.0	41.0
47	35.60599935084864	39.0	35.0	41.0	27.0	41.0
48	35.5469177454576	38.0	35.0	41.0	27.0	41.0
49	35.48909690681587	38.0	35.0	41.0	27.0	41.0
50	35.36432847058616	38.0	35.0	40.0	26.0	41.0
51	35.27125805091201	38.0	35.0	40.0	26.0	41.0
52	34.91529789202449	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	11.0
16	20.0
17	53.0
18	165.0
19	325.0
20	629.0
21	980.0
22	1629.0
23	2857.0
24	5334.0
25	9639.0
26	13880.0
27	14684.0
28	13488.0
29	12989.0
30	14145.0
31	16050.0
32	20114.0
33	26240.0
34	47260.0
35	82878.0
36	77943.0
37	72714.0
38	122668.0
39	348817.0
40	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.047200369177233	19.193683359866107	25.171727029646785	28.587389241309875
2	33.153087112137584	19.574010982581104	26.48559612628864	20.787305778992668
3	22.4287313506985	22.13440524267	36.26746802267172	19.169395383959777
4	23.618510970437118	24.51451648159965	33.417826049516556	18.449146498446673
5	20.925084842315837	30.099867740931202	30.086730153963686	18.888317262789275
6	75.67647532893648	1.6289503840812192	19.165641787683345	3.5289324992989606
7	76.19204281749352	1.2171587925784777	16.149516779679356	6.441281610248642
8	65.20184411977064	3.3614558654357816	23.378612008416887	8.058088006376698
9	33.78888008143096	23.506786281268006	26.719533494222773	15.98480014307826
10	24.601069995738563	19.575666980938355	42.95814298552216	12.865120037800923
11	22.884793298285047	17.56440177611344	39.44102327450491	20.109781651096604
12	17.735852806034018	21.470129101631933	43.14670599846765	17.6473120938664
13	14.090669222056132	24.586497210194768	41.43561809586685	19.887215471882254
14	13.559976948502866	22.36801141093268	44.872366686612246	19.199644953952205
15	13.79932391107068	20.62490754009172	45.63721712788061	19.93855142095699
16	13.627100081916717	24.089918502800845	38.84088946983765	23.44209194544479
17	15.704053221579203	21.385231585850267	40.737228388669436	22.173486803901092
18	16.77239296178618	27.11940189755332	39.789666128651206	16.3185390120093
19	17.58350095716705	23.16046182482187	36.062345026153736	23.193692191857345
20	15.146312974857528	28.64667398249941	35.975349912452884	20.231663130190174
21	15.1987529228371	23.05536112908176	36.59226450047362	25.15362144760752
22	12.893272009874165	26.153402855824364	34.15463491860216	26.798690215699306
23	12.821180881388566	22.91923806411584	42.42159951777328	21.837981536722314
24	16.914698420619168	21.824623149973835	39.08255483010561	22.17812359930139
25	13.947259764318314	25.460643543041606	38.28679241950192	22.30530427313816
26	17.282330055928586	21.745356028606817	36.27519601500555	24.69711790045904
27	21.487903483999744	22.03935093696387	38.47568663211886	17.997058946917523
28	16.871532063440192	22.05999571648425	35.65739822786096	25.4110739922146
29	21.42939154204359	22.81071497177075	34.124937348062154	21.634956138123513
30	23.95754903411136	21.766332007798646	32.463418996288354	21.812699961801638
31	22.113870863040102	20.4140437492686	36.504386187648905	20.967699200042393
32	22.71499826672172	24.20274719087479	31.67361817977077	21.408636362632727
33	20.350122212678766	20.41371254959715	38.43218907526843	20.803976162455648
34	18.172263573114535	22.335443443240106	39.39399292115902	20.098300062486338
35	19.19412495942804	23.41007597720463	36.36296392793979	21.032835135427547
36	17.863254279651755	27.513419106688247	36.96111053457835	17.66221607908165
37	15.699968425631322	22.81954696300941	41.362091768804966	20.1183928425543
38	14.88775643134562	25.051501548910466	35.05053002987421	25.010211989869706
39	20.312807049695405	23.81712037341659	34.7946230837339	21.075449493154103
40	16.825826508780104	21.10227666654155	38.028898275332914	24.04299854934544
41	21.0807486878973	22.73332464854195	31.560127092353923	24.625799571206823
42	20.517267646870494	20.387547775552605	35.59458069017595	23.500603887400946
43	22.568056012488434	23.001154782854456	34.64293363420983	19.787855570447274
44	17.12291261407069	24.127233665784203	36.258415231652094	22.49143848849302
45	19.833340325326397	21.3889851821267	35.52436635982857	23.25330813271833
46	22.623035157949122	19.76235319574563	34.89961337958353	22.71499826672172
47	17.746892795082346	19.480943874903677	37.66656583476669	25.105597495247284
48	18.710352639330182	18.09730204747637	33.76911850103445	29.423226812159005
49	20.079532081104176	18.271954674220964	38.19891410667721	23.449599137997655
50	19.22890092493028	17.05888067759037	38.2975012088788	25.414717188600548
51	16.997719138262614	16.50213402988304	37.72375297803705	28.776393853817297
52	16.214652715064506	16.846029688738547	43.673534275853996	23.265783320342948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	909.0
2	1619.0
3	24286.0
4	46953.0
5	26463.5
6	5974.0
7	5934.0
8	5894.0
9	6072.5
10	6251.0
11	6453.5
12	6656.0
13	6502.5
14	6212.0
15	6075.0
16	5545.0
17	5015.0
18	4683.5
19	4352.0
20	4179.5
21	4007.0
22	3928.0
23	3849.0
24	4154.5
25	4460.0
26	4845.5
27	5231.0
28	5570.0
29	5909.0
30	6620.0
31	7331.0
32	8530.5
33	9730.0
34	11232.0
35	12734.0
36	14574.0
37	16414.0
38	18695.0
39	24685.5
40	28395.0
41	38941.0
42	49487.0
43	66120.5
44	82754.0
45	104916.5
46	127079.0
47	130803.5
48	134528.0
49	118663.5
50	102799.0
51	83435.5
52	64072.0
53	51226.0
54	38380.0
55	31715.5
56	25051.0
57	21747.0
58	18443.0
59	16334.0
60	14225.0
61	11697.0
62	9169.0
63	7801.0
64	5550.0
65	4667.0
66	4019.0
67	3371.0
68	2890.5
69	2410.0
70	2281.5
71	2153.0
72	2285.5
73	2418.0
74	2022.5
75	1627.0
76	1324.5
77	1022.0
78	866.5
79	711.0
80	532.0
81	353.0
82	244.5
83	136.0
84	108.5
85	81.0
86	53.5
87	26.0
88	22.5
89	10.5
90	2.0
91	4.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	905798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.63038252328534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04195264266548	23.50756472454582
2	11.956602640456888	7.802970356740636
3	5.056021172611098	4.949397147243895
4	2.6610068495874444	3.4731868559648293
5	1.5646160907597777	2.552701077178944
6	0.9946180508769781	1.947286047880816
7	0.7021729950081507	1.603852139724582
8	0.5219491260300685	1.3625119712044484
9	0.39818783975241223	1.169371937451767
>10	3.5310908749895087	24.770302158133457
>50	0.41064943596971754	9.069498267596877
>100	0.15025415259766597	8.54083208193555
>500	0.006458888912568665	1.451998025280586
>1k	0.0033994152171414028	2.0595299721455036
>5k	6.798830434282806E-4	1.2964822703956829
>10k+	3.399415217141403E-4	4.442514966576583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40050	4.421515613856511	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	6361	0.7022537033643262	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	5327	0.5881002166045851	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	4248	0.4689787347730951	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2570	0.2837277185421032	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1892	0.20887659279441995	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	1890	0.20865579301345333	No Hit
CGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1691	0.1866862148072749	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1413	0.1559950452529151	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1391	0.15356624766228233	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	1310	0.1446238565331343	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1118	0.12342707756033905	No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1044	0.11525748566457422	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	958	0.10576309508300967	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6090761957964138	0.0
2	0.0	0.0	0.0	2.1556682615770844	0.0
3	0.0	0.0	0.0	3.513255714850331	0.0
4	0.0	0.0	0.0	4.471085164683517	0.0
5	0.0	0.0	0.0	8.809469661006096	0.0
6	0.0	0.0	0.0	9.853079825744812	0.0
7	0.0	0.0	0.0	13.636263272826833	0.0
8	0.0	0.0	0.0	18.036140508148616	0.0
9	0.0	0.0	0.0	21.70263127098978	0.0
10	0.0	0.0	0.0	23.78046760977613	0.0
11	0.0	0.0	0.0	25.792836813505883	0.0
12	0.0	0.0	0.0	27.267448150691436	0.0
13	1.1039989048330864E-4	0.0	0.0	27.91361870969024	0.0
14	1.1039989048330864E-4	0.0	0.0	28.377850249172553	0.0
15	2.207997809666173E-4	0.0	0.0	29.829277609356613	0.0
16	2.207997809666173E-4	0.0	0.0	31.589603863112966	0.0
17	2.207997809666173E-4	0.0	0.0	33.65430261493181	0.0
18	2.207997809666173E-4	0.0	0.0	34.77066630749902	0.0
19	2.207997809666173E-4	0.0	0.0	35.92191636545896	0.0
20	3.3119967144992594E-4	0.0	0.0	37.11434558256918	0.0
21	3.3119967144992594E-4	0.0	0.0	38.306995599460365	0.0
22	9.935990143497778E-4	0.0	0.0	39.570963945603765	0.0
23	0.0014351985762830124	0.0	0.0	40.551977372438444	0.0
24	0.00242879759063279	0.0	0.0	41.33857659213202	0.0
25	0.00242879759063279	0.0	0.0	42.08454865212774	0.0
26	0.0025391974811160988	0.0	0.0	42.83449510818085	0.0
27	0.0025391974811160988	0.0	0.0	43.57980476883367	0.0
28	0.0025391974811160988	0.0	0.0	44.31749683704314	0.0
29	0.0025391974811160988	0.0	0.0	45.230724731121065	0.0
30	0.0025391974811160988	0.0	0.0	46.00926475880936	0.0
31	0.0025391974811160988	0.0	0.0	46.65731211594638	0.0
32	0.0025391974811160988	0.0	0.0	47.32710825150861	0.0
33	0.0025391974811160988	0.0	0.0	48.006067577980964	0.0
34	0.0025391974811160988	0.0	0.0	48.74022684969496	0.0
35	0.0025391974811160988	0.0	0.0	49.382975012088785	0.0
36	0.0025391974811160988	0.0	0.0	50.01810558203926	0.0
37	0.0025391974811160988	0.0	0.0	50.645508159655904	0.0
38	0.0025391974811160988	0.0	0.0	51.251161958847334	0.0
39	0.0025391974811160988	0.0	0.0	51.8824285326309	0.0
40	0.0025391974811160988	0.0	0.0	52.49569992426567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	70	0.0	46.000004	1
GAATAGT	35	1.0192525E-7	46.000004	8
TTACCGG	35	1.0192525E-7	46.000004	1
AGCGTTG	20	6.3107436E-4	46.0	30
AACGTGC	20	6.3107436E-4	46.0	13
CGGCGAT	40	5.6097633E-9	46.0	5
CTCACGC	20	6.3107436E-4	46.0	46
ACCGGCC	40	5.6097633E-9	46.0	20
TCCGAGG	40	5.6097633E-9	46.0	1
ACGCACC	20	6.3107436E-4	46.0	20
CGTGAGT	20	6.3107436E-4	46.0	31
GCCGGTC	40	5.6097633E-9	46.0	31
AGGGTCG	50	1.6370905E-11	46.0	5
CACGACC	30	1.8609626E-6	46.0	26
ACGGGTC	40	5.6097633E-9	46.0	4
CGTTCCG	20	6.3107436E-4	46.0	18
AGTATAC	25	3.4165787E-5	46.0	30
CGTTAGC	20	6.3107436E-4	46.0	18
ACGATGG	20	6.3107436E-4	46.0	1
GCCGATG	30	1.8609626E-6	46.0	8
>>END_MODULE