##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043213_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	891992
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.204025372424866	33.0	31.0	34.0	30.0	34.0
2	32.500836330370674	34.0	31.0	34.0	31.0	34.0
3	32.583824742822806	34.0	31.0	34.0	31.0	34.0
4	36.126258979901166	37.0	35.0	37.0	35.0	37.0
5	36.071446829119544	37.0	35.0	37.0	35.0	37.0
6	36.31357231903425	37.0	37.0	37.0	35.0	37.0
7	36.254552731414634	37.0	37.0	37.0	35.0	37.0
8	36.158770482246474	37.0	37.0	37.0	35.0	37.0
9	37.688846985174756	39.0	38.0	39.0	35.0	39.0
10	37.473799092368544	39.0	37.0	39.0	35.0	39.0
11	37.28193077964825	39.0	37.0	39.0	34.0	39.0
12	37.29784459950314	39.0	37.0	39.0	34.0	39.0
13	37.322321276424006	39.0	37.0	39.0	34.0	39.0
14	38.69899730042422	40.0	38.0	41.0	35.0	41.0
15	38.78428057650741	40.0	38.0	41.0	35.0	41.0
16	38.82789307527422	40.0	38.0	41.0	35.0	41.0
17	38.80926398443035	40.0	38.0	41.0	35.0	41.0
18	38.59313984878788	40.0	38.0	41.0	35.0	41.0
19	38.37533072045489	40.0	37.0	41.0	35.0	41.0
20	38.121029112368724	40.0	35.0	41.0	34.0	41.0
21	38.06429429860358	40.0	35.0	41.0	34.0	41.0
22	38.012064009542684	40.0	35.0	41.0	34.0	41.0
23	37.98748643485592	40.0	35.0	41.0	34.0	41.0
24	37.907615763370075	40.0	35.0	41.0	34.0	41.0
25	37.89147996843021	40.0	35.0	41.0	34.0	41.0
26	37.87153023793935	40.0	35.0	41.0	34.0	41.0
27	37.85490564937802	40.0	35.0	41.0	34.0	41.0
28	37.72630584130799	40.0	35.0	41.0	34.0	41.0
29	37.67906999165912	40.0	35.0	41.0	34.0	41.0
30	37.572822401994635	40.0	35.0	41.0	34.0	41.0
31	37.28450479376497	39.0	35.0	41.0	33.0	41.0
32	37.131899165014936	40.0	35.0	41.0	33.0	41.0
33	36.804919775065244	39.0	35.0	41.0	31.0	41.0
34	36.44823159848967	39.0	35.0	41.0	31.0	41.0
35	36.28906537278361	39.0	35.0	41.0	30.0	41.0
36	36.28225365249912	39.0	35.0	41.0	30.0	41.0
37	36.26659768249043	39.0	35.0	41.0	30.0	41.0
38	36.22879913721199	39.0	35.0	41.0	30.0	41.0
39	36.20416438712455	39.0	35.0	41.0	30.0	41.0
40	36.18712611772303	39.0	35.0	41.0	30.0	41.0
41	36.08009600982968	39.0	35.0	41.0	30.0	41.0
42	35.93035027219975	39.0	35.0	41.0	29.0	41.0
43	35.76316827953614	39.0	35.0	41.0	28.0	41.0
44	35.683840213813575	39.0	35.0	40.0	28.0	41.0
45	35.65842182441098	39.0	35.0	40.0	27.0	41.0
46	35.562473654472235	38.0	35.0	40.0	27.0	41.0
47	35.40972901102252	38.0	35.0	40.0	26.0	41.0
48	35.373156934142905	38.0	35.0	40.0	26.0	41.0
49	35.30497246612077	38.0	35.0	40.0	26.0	41.0
50	35.21629341967193	38.0	35.0	40.0	26.0	41.0
51	34.763877927156294	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	1.0
15	8.0
16	28.0
17	49.0
18	133.0
19	248.0
20	483.0
21	836.0
22	1410.0
23	2315.0
24	4264.0
25	8242.0
26	12606.0
27	14781.0
28	14438.0
29	13405.0
30	14159.0
31	16374.0
32	19905.0
33	25839.0
34	47362.0
35	81329.0
36	75059.0
37	72488.0
38	129714.0
39	336476.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.391949703584785	19.20902878052718	25.328478282316436	28.070543233571605
2	32.88370299285195	19.67315850366371	26.487793612498766	20.955344890985568
3	22.629687261769163	22.17486255482112	35.92767648140342	19.267773702006295
4	23.77543744786949	24.72174638337564	32.888972098404466	18.613844070350407
5	21.064987129929417	30.28143750168163	29.723360747630025	18.93021462075893
6	76.18666983560391	1.586000771307366	18.705885254576273	3.521444138512453
7	76.6223239670311	1.1673871514542733	15.733437071184495	6.476851810330138
8	65.49643942995004	3.2787289572103786	23.012874554928743	8.211957057910833
9	33.91812931057678	23.544829998475322	26.400685207939084	16.136355483008817
10	24.87522309617127	19.556565529735693	42.60296056466874	12.965250809424301
11	23.112090691396336	17.43816088036664	39.15887138001238	20.290877048224647
12	17.960362873209625	21.395931802078945	42.862940474802464	17.780764849908966
13	14.251136781495799	24.499322863882185	41.1169606902304	20.13257966439161
14	13.68655772697513	22.198517475493055	44.74042368092987	19.37450111660194
15	13.882859935963552	20.566440057758363	45.44917443205769	20.101525574220396
16	13.635884626767952	23.94573045498166	38.77702939039812	23.641355527852266
17	15.886689566722573	21.31263509089768	40.51617054861479	22.284504793764967
18	16.915510453008547	27.198898644830894	39.60203678956762	16.283554112592938
19	17.70823056709029	23.156037273876894	35.799872644597706	23.335859514435107
20	15.1427367061588	28.741849702687915	35.69572372846393	20.419689862689353
21	15.151032744688292	23.113660212199214	36.216468309132814	25.518838733979678
22	12.948210297850204	26.20124395734491	33.8674562103696	26.983089534435283
23	12.976125346415662	22.90099014340936	42.0605790186459	22.06230549152907
24	16.983224064789816	21.85468031103418	38.793733576085884	22.36836204809012
25	13.91436246064987	25.346415662920744	38.176463466040055	22.562758410389332
26	17.40957318002852	21.700530946465886	36.0361976340595	24.853698239446093
27	21.58393797253787	21.968022134727665	38.26805621575081	18.17998367698365
28	17.06237275670634	22.027776033865777	35.42094547933166	25.488905730096235
29	21.599408963309088	22.742916976833875	33.71756697369483	21.940107086162207
30	24.14461116243195	21.629790401707638	32.14557978098458	22.08001865487583
31	22.390895882474283	20.304666409564213	36.12958412183069	21.17485358613082
32	23.038435322289885	24.06333240656867	31.228194871702886	21.67003739943856
33	20.701530955434578	20.179889505735478	38.20953551152925	20.909044027300695
34	18.482004322908725	22.19022143696356	39.21257141319653	20.115202826931185
35	19.297482488632184	23.362653476712797	36.274764796096825	21.065099238558194
36	17.935811083507474	27.476928044197706	36.97678902949802	17.610471842796795
37	15.889380173813217	22.616122117687155	41.305303186575664	20.189194521923966
38	14.911008170476867	24.945403097785633	34.98080700275339	25.16278172898412
39	20.40769423941022	23.69729773361196	34.75849559188872	21.136512435089102
40	16.89017390290496	21.04278962143158	37.916707773163886	24.150328702499575
41	21.16151265930636	22.580920008251194	31.460483950528705	24.79708338191374
42	20.83662185311079	20.175629377841954	35.34560848079355	23.642140288253707
43	22.713208190207983	22.797401770419466	34.56880779199813	19.920582247374416
44	17.1729118646804	24.022749082951417	36.26086332612849	22.5434757262397
45	20.111951676696652	21.073507385716464	35.493143436264	23.32139750132288
46	22.8789047435403	19.580220450407626	34.770715432425405	22.77015937362667
47	17.941304406317546	19.192773029354523	37.661436425438794	25.204486138889138
48	18.87528139265823	17.8938824563449	33.605009910402785	29.625826240594087
49	20.192894106673602	17.99747082933479	38.20370586283285	23.605929201158755
50	19.42371680463502	16.747011183956808	38.23767477735227	25.591597234055914
51	17.15205965972789	16.37167149481161	37.531614633315094	28.944654212145398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	827.0
2	1481.0
3	22856.0
4	44231.0
5	25281.0
6	6331.0
7	6116.0
8	5901.0
9	6075.0
10	6249.0
11	6363.5
12	6478.0
13	6249.5
14	6021.0
15	5705.5
16	5390.0
17	4911.5
18	4433.0
19	4191.0
20	3949.0
21	3847.5
22	3746.0
23	3545.0
24	3344.0
25	3920.0
26	4598.0
27	4700.0
28	5466.5
29	6233.0
30	7006.5
31	7780.0
32	9096.0
33	10412.0
34	11735.5
35	13059.0
36	15703.0
37	18347.0
38	20671.0
39	22995.0
40	29144.5
41	35294.0
42	45467.5
43	55641.0
44	76090.5
45	96540.0
46	117813.0
47	139086.0
48	137546.0
49	136006.0
50	110659.5
51	85313.0
52	67705.0
53	50097.0
54	40444.0
55	30791.0
56	26352.5
57	21914.0
58	19008.0
59	16102.0
60	13452.0
61	10802.0
62	9217.5
63	7633.0
64	6444.5
65	5256.0
66	4726.5
67	4197.0
68	3452.5
69	2708.0
70	2402.5
71	2097.0
72	2128.0
73	2159.0
74	2009.0
75	1549.0
76	1239.0
77	1029.0
78	819.0
79	590.5
80	362.0
81	268.0
82	174.0
83	137.5
84	101.0
85	62.0
86	23.0
87	21.0
88	19.0
89	12.5
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	891992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.02044026911077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.19113545687065	22.79571500586901
2	12.437149504120624	7.964860056293903
3	5.132342438562558	4.930195934838442
4	2.639910320282714	3.3812436290568697
5	1.6193024809686152	2.5925389184739216
6	1.03854828546578	1.9952864004806645
7	0.7164340586574542	1.6058373787398332
8	0.5525565104304252	1.4154482190036568
9	0.41357393488553823	1.1918537530977138
>10	3.670051122342081	25.258498351778364
>50	0.4236389258920574	9.11984586822857
>100	0.15405050261400832	8.672502172973205
>500	0.007066536817156101	1.5795030048513008
>1k	0.0031799415677202453	1.9582850448371294
>5k	7.066536817156101E-4	1.2799167318875986
>10k+	3.5332684085780504E-4	4.2584695295897825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37640	4.219768787164011	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	6131	0.6873380030314173	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	5182	0.5809469143221015	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	4184	0.4690625028027157	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	2468	0.27668409582148723	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2002	0.22444147481143328	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	1814	0.20336505260136864	No Hit
CGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	1716	0.19237840698122852	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1417	0.15885792697692355	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1362	0.15269195239419187	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	1187	0.13307294235822742	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1159	0.1299339007524731	No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	994	0.11143597700427807	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	960	0.10762428362586213	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	923	0.10347626436111533	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5982116431537503	0.0
2	0.0	0.0	0.0	2.145310720275518	0.0
3	0.0	0.0	0.0	3.5131480999829594	0.0
4	0.0	0.0	0.0	4.510466461582615	0.0
5	0.0	0.0	0.0	8.881133463080387	0.0
6	0.0	0.0	0.0	9.949080260809515	0.0
7	0.0	0.0	0.0	13.822657602310334	0.0
8	0.0	0.0	0.0	18.353752051587907	0.0
9	1.121086287769397E-4	0.0	0.0	22.160736867595226	0.0
10	1.121086287769397E-4	0.0	0.0	24.30941084673405	0.0
11	1.121086287769397E-4	0.0	0.0	26.372209616229743	0.0
12	1.121086287769397E-4	0.0	0.0	27.90092287823209	0.0
13	1.121086287769397E-4	0.0	0.0	28.56404541744769	0.0
14	1.121086287769397E-4	0.0	0.0	29.042300827810116	0.0
15	1.121086287769397E-4	0.0	0.0	30.552404057435492	0.0
16	1.121086287769397E-4	0.0	0.0	32.33694921030682	0.0
17	1.121086287769397E-4	0.0	0.0	34.45187849218379	0.0
18	1.121086287769397E-4	0.0	0.0	35.58574516363375	0.0
19	1.121086287769397E-4	0.0	0.0	36.74169723495278	0.0
20	1.121086287769397E-4	0.0	0.0	37.982851864142276	0.0
21	1.121086287769397E-4	0.0	0.0	39.21896160503682	0.0
22	6.726517726616383E-4	0.0	0.0	40.48343482901192	0.0
23	0.0012331949165463368	0.0	0.0	41.500932743791424	0.0
24	0.0021300639467618544	0.0	0.0	42.3202226028933	0.0
25	0.0021300639467618544	0.0	0.0	43.079758562857066	0.0
26	0.0021300639467618544	0.0	0.0	43.83570704669997	0.0
27	0.0021300639467618544	0.0	0.0	44.574727127597555	0.0
28	0.0021300639467618544	0.0	0.0	45.31924053130521	0.0
29	0.0021300639467618544	0.0	0.0	46.25019058466892	0.0
30	0.0021300639467618544	0.0	0.0	47.03674472416793	0.0
31	0.0021300639467618544	0.0	0.0	47.69392550605835	0.0
32	0.0021300639467618544	0.0	0.0	48.35032152754733	0.0
33	0.0021300639467618544	0.0	0.0	49.028354514390266	0.0
34	0.0021300639467618544	0.0	0.0	49.76513242271231	0.0
35	0.0021300639467618544	0.0	0.0	50.41805307670921	0.0
36	0.0021300639467618544	0.0	0.0	51.0520273724428	0.0
37	0.0021300639467618544	0.0	0.0	51.690037578812365	0.0
38	0.0021300639467618544	0.0	0.0	52.2931820016323	0.0
39	0.0021300639467618544	0.0	0.0	52.90798572184504	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	20	7.0324366E-4	45.0	1
ACTGCGG	70	0.0	45.0	1
CGTGCGG	20	7.0324366E-4	45.0	1
TAGACGG	90	0.0	45.0	1
CGCATTG	20	7.0324366E-4	45.0	31
GGCGTAC	70	0.0	45.0	7
TAAACGG	50	2.1827873E-11	45.0	1
TCGATTG	25	3.8901002E-5	45.0	13
TCGATGG	95	0.0	45.0	1
TTATCGG	20	7.0324366E-4	45.0	1
GCCGATG	45	3.8380676E-10	45.0	8
TCTACGA	40	6.8121153E-9	45.0	10
ACAGCGC	25	3.8901002E-5	45.0	39
CGTACTC	55	1.8189894E-12	45.0	9
GCGATCG	35	1.2115197E-7	45.0	8
TACTCGA	25	3.8901002E-5	45.0	20
CGGCTTT	20	7.0324366E-4	45.0	27
CGGTCTA	80	0.0	45.0	30
CAGTATA	20	7.0324366E-4	45.0	26
CTCTCGA	20	7.0324366E-4	45.0	31
>>END_MODULE