FastQCFastQC Report
Thu 26 May 2016
SRR1043212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043212_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1036695
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT548965.295289357043297No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT62050.5985366959423939No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT52500.5064170271873598Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT35060.33819011377502545No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC33320.32140600658824436No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG19430.18742253025238859No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT16900.16301805256126442No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT16870.16272867140287162No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC13970.13475515942490318No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC13260.12790647200960745No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC11710.11295511215931398No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC10610.10234446968491215No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT10440.10070464312068642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG1050.046.0000041
CTAACGG1050.046.0000041
ACCGGCG600.046.019
AGATCCG206.311168E-446.024
GACCGAC206.311168E-446.08
ACTACGG501.6370905E-1146.01
AATTCGG405.6097633E-946.01
TCATCGG405.6097633E-946.01
TCGGTTG206.311168E-446.028
AGTACGG453.092282E-1046.01
ACGGTGA206.311168E-446.026
GCGAGTA301.8612245E-646.021
TCACGAC1400.045.99999624
CTCGTCC351.0194344E-745.99999636
CCGTGTA351.0194344E-745.99999624
CATCCGG351.0194344E-745.9999961
CGTTTTT124450.045.5194851
TTCACGG2250.044.977781
CGGTCTA1350.044.296330
ATCGCGG1100.043.9090921
Produced by FastQC (version 0.11.5)