##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043212_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1036695
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.307083568455525	33.0	31.0	34.0	31.0	34.0
2	32.63274154886442	34.0	31.0	34.0	31.0	34.0
3	32.72063721731078	34.0	31.0	34.0	31.0	34.0
4	36.21374656962752	37.0	37.0	37.0	35.0	37.0
5	36.11449944294127	37.0	35.0	37.0	35.0	37.0
6	36.344325958936814	37.0	37.0	37.0	35.0	37.0
7	36.276709157466755	37.0	37.0	37.0	35.0	37.0
8	36.24854947694356	37.0	37.0	37.0	35.0	37.0
9	37.78366250440101	39.0	38.0	39.0	35.0	39.0
10	37.56961015534945	39.0	37.0	39.0	35.0	39.0
11	37.36547489859602	39.0	37.0	39.0	35.0	39.0
12	37.38587337645112	39.0	37.0	39.0	35.0	39.0
13	37.38896493182662	39.0	37.0	39.0	35.0	39.0
14	38.800820877885975	40.0	38.0	41.0	35.0	41.0
15	38.87332436251742	41.0	38.0	41.0	35.0	41.0
16	38.875587323176056	41.0	38.0	41.0	35.0	41.0
17	38.84631159598532	41.0	38.0	41.0	35.0	41.0
18	38.6039664510777	40.0	38.0	41.0	35.0	41.0
19	38.337096252996304	40.0	37.0	41.0	35.0	41.0
20	38.04934141671369	40.0	35.0	41.0	34.0	41.0
21	37.97955425655569	40.0	35.0	41.0	34.0	41.0
22	37.92466443843175	40.0	35.0	41.0	34.0	41.0
23	37.93755347522656	40.0	35.0	41.0	34.0	41.0
24	37.85859003853592	40.0	35.0	41.0	34.0	41.0
25	37.76529934069326	40.0	35.0	41.0	34.0	41.0
26	37.6977249817931	40.0	35.0	41.0	34.0	41.0
27	37.7104423191006	40.0	35.0	41.0	34.0	41.0
28	37.68605809809057	40.0	35.0	41.0	34.0	41.0
29	37.65200275876704	40.0	35.0	41.0	34.0	41.0
30	37.507223436015416	40.0	35.0	41.0	33.0	41.0
31	37.40433203594114	40.0	35.0	41.0	33.0	41.0
32	37.12302461186752	40.0	35.0	41.0	33.0	41.0
33	36.87114049937542	40.0	35.0	41.0	32.0	41.0
34	36.632114556354566	40.0	35.0	41.0	31.0	41.0
35	36.50263288623945	40.0	35.0	41.0	31.0	41.0
36	36.44073425645923	40.0	35.0	41.0	31.0	41.0
37	36.401003188015764	40.0	35.0	41.0	30.0	41.0
38	36.35404144902792	40.0	35.0	41.0	30.0	41.0
39	36.316345694731815	40.0	35.0	41.0	30.0	41.0
40	36.247678439656795	40.0	35.0	41.0	30.0	41.0
41	36.20587250830765	40.0	35.0	41.0	30.0	41.0
42	36.09634945668687	39.0	35.0	41.0	30.0	41.0
43	35.9831888839051	39.0	35.0	41.0	29.0	41.0
44	35.82436492893281	39.0	35.0	41.0	28.0	41.0
45	35.74100000482302	39.0	35.0	41.0	28.0	41.0
46	35.69544562286883	39.0	35.0	41.0	28.0	41.0
47	35.59472168767092	39.0	35.0	41.0	27.0	41.0
48	35.534623973299766	38.0	35.0	41.0	27.0	41.0
49	35.446247932130476	38.0	35.0	40.0	27.0	41.0
50	35.277820381114985	38.0	35.0	40.0	26.0	41.0
51	35.155873231760545	38.0	35.0	40.0	26.0	41.0
52	34.78215000554647	37.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	4.0
15	10.0
16	23.0
17	81.0
18	176.0
19	418.0
20	696.0
21	1161.0
22	1973.0
23	3204.0
24	6078.0
25	11452.0
26	15784.0
27	17038.0
28	15713.0
29	14671.0
30	15462.0
31	17999.0
32	21856.0
33	28844.0
34	51795.0
35	92848.0
36	93092.0
37	77555.0
38	134993.0
39	412839.0
40	928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.364842118463	19.174877857036062	25.796401062993457	28.663878961507482
2	33.14041256107148	20.542493211600327	26.096103482702244	20.22099074462595
3	21.653331018284067	21.795224246282658	36.32177255605554	20.229672179377733
4	22.58234099711101	24.28679602004447	34.34250189303508	18.788361089809445
5	20.741683908960688	30.43604917550485	30.443380164850797	18.378886750683662
6	76.15711467693005	1.518672319245294	19.15384949285952	3.1703635109651342
7	76.6987397450552	1.2099026232402008	16.430386950838965	5.660970680865636
8	67.0010948253826	3.418749005252268	22.552245356638164	7.027910812726984
9	32.15304404863532	26.790521802458777	26.02607324237119	15.030360906534709
10	23.04264995972779	20.294686479629977	43.77594181509509	12.886721745547147
11	21.584265381814323	17.75006149349616	40.91347985665987	19.752193268029654
12	17.71977293225105	21.80390568103444	42.58320914058619	17.89311224612832
13	14.70326373716474	25.178186448280353	40.99508534332663	19.123464471228278
14	13.21941361731271	23.093195202060393	44.31004297310202	19.377348207524875
15	14.28848407680176	20.29487940040224	45.5435783909443	19.8730581318517
16	14.699598242491765	24.014584810382996	38.94028619796565	22.34553074915959
17	15.07106718948196	22.21762427714998	41.74236395468291	20.968944578685146
18	16.97394122668673	26.85553610271102	39.36230038728845	16.8082222833138
19	17.266891419366353	23.782886962896512	37.01889176662374	21.931329851113393
20	15.076083129560768	28.164985844438334	36.48971008830949	20.26922093769141
21	15.43250425631454	22.95718605761579	37.543250425631456	24.06705926043822
22	13.298993435870724	25.134393432976914	35.58510458717366	25.981508543978705
23	12.633223850795074	22.442087595676647	43.598551165000316	21.326137388527965
24	16.690444151847938	22.491089471831156	39.19677436468778	21.621692011633122
25	14.995056405210791	25.5155084185802	38.302104283323445	21.187330892885566
26	16.12547567027911	23.29923458683605	37.401935959949654	23.173353782935195
27	19.650234639889263	23.467365039862255	38.49087725898166	18.391523061266817
28	16.30720703774977	23.19939808719054	35.52906110283159	24.96433377222809
29	20.31571484380652	22.567389637260717	34.482948215241706	22.633947303691055
30	19.818558013687728	25.06812514770497	32.83472959742258	22.278587241184727
31	23.67407964734083	22.064831025518593	31.731705082015445	22.529384245125133
32	19.352847269447622	24.48569733624644	31.209757932661002	24.95169746164494
33	18.827138165034075	23.98053429407878	34.60670689064768	22.585620650239463
34	18.37444957292164	22.761660854928405	32.14397677233902	26.719912799810935
35	19.908652014334013	21.629891144454252	33.14176300647731	25.31969383473442
36	16.979535929082324	25.55081291990412	33.055045119345614	24.414606031667947
37	16.25685471618943	22.861690275346174	35.43896710218531	25.442487906279087
38	15.713589821500054	24.373803288334564	31.415893777822795	28.496713112342587
39	20.207486290567623	22.92419660555901	33.333236872947204	23.535080230926162
40	16.211614795094025	22.098399239892156	36.40173821615808	25.288247748855742
41	20.20198804855816	24.06812032468566	30.33901002705714	25.390881599699043
42	19.984759258991314	21.45905980061638	34.097396051876395	24.458784888515908
43	22.00531496727581	22.756355533691202	33.43316983297884	21.805159666054145
44	17.157698262266145	24.058377825686435	34.0237967772585	24.76012713478892
45	19.496283863624306	21.881170450325314	33.77338561486262	24.849160071187764
46	21.687960296905068	21.2326672743671	32.01047559793382	25.068896830794014
47	17.569873492203588	20.580209222577516	35.40028648734681	26.449630797872082
48	18.705115776578456	18.96951369496332	32.84148182445175	29.483888704006482
49	19.645218699810453	19.038772252205323	36.24643699448729	25.06957205349693
50	18.550007475679926	18.35438581260641	36.659769749058306	26.43583696265536
51	16.691505216095383	18.063557748421665	35.85123879250889	29.393698242974065
52	16.09354728246977	18.30451579297672	41.112574093634095	24.48936283091941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	1346.0
2	2395.0
3	32572.0
4	62749.0
5	35068.0
6	7387.0
7	7144.5
8	6902.0
9	7183.0
10	7464.0
11	7570.0
12	7676.0
13	7602.0
14	7140.0
15	6752.0
16	6296.5
17	5841.0
18	5428.0
19	5015.0
20	4866.5
21	4718.0
22	4793.0
23	4868.0
24	5135.5
25	5403.0
26	6572.5
27	7742.0
28	8142.0
29	8542.0
30	9634.0
31	10726.0
32	12419.5
33	14113.0
34	17476.0
35	20839.0
36	21968.0
37	23097.0
38	24943.5
39	28914.0
40	31038.0
41	35779.5
42	40521.0
43	47619.5
44	54718.0
45	61556.0
46	68394.0
47	79756.0
48	91118.0
49	109373.5
50	127629.0
51	129404.5
52	131180.0
53	114466.5
54	97753.0
55	77117.0
56	56481.0
57	44170.5
58	31860.0
59	25683.5
60	19507.0
61	15636.5
62	11766.0
63	9892.0
64	6662.5
65	5307.0
66	4449.5
67	3592.0
68	3122.5
69	2653.0
70	2399.0
71	2145.0
72	2375.0
73	2605.0
74	2100.5
75	1596.0
76	1345.0
77	1094.0
78	789.0
79	484.0
80	359.5
81	235.0
82	156.5
83	78.0
84	57.5
85	37.0
86	34.0
87	31.0
88	17.0
89	3.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1036695.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.48028953131004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93920395558095	24.755059561474347
2	11.559458041595667	7.740280041153061
3	4.4823150427307965	4.502076162035202
4	2.1881567530172563	2.9304048652363606
5	1.3294742092298077	2.2255590724711714
6	0.8500778324468831	1.7076511172682065
7	0.6250139941396425	1.4647954639441023
8	0.46233437999451305	1.2383271121996011
9	0.36353346151743443	1.0954084991330755
>10	3.6887260274716684	27.200970375474203
>50	0.3777778284717154	8.404016050656072
>100	0.12380429111898135	7.030042171145107
>500	0.006074509610846177	1.4220848496998886
>1k	0.003181885986633712	1.857503910190947
>5k	5.785247248424931E-4	1.1093695146630498
>10k+	2.8926236242124653E-4	5.316451233255591
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54896	5.295289357043297	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	6205	0.5985366959423939	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT	5250	0.5064170271873598	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	3506	0.33819011377502545	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	3332	0.32140600658824436	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	1943	0.18742253025238859	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	1690	0.16301805256126442	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	1687	0.16272867140287162	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1397	0.13475515942490318	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1326	0.12790647200960745	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1171	0.11295511215931398	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1061	0.10234446968491215	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	1044	0.10070464312068642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5204037831763441	0.0
2	0.0	0.0	0.0	1.7401453658018993	0.0
3	0.0	0.0	0.0	2.9510125929034094	0.0
4	0.0	0.0	0.0	3.704175287813677	0.0
5	0.0	0.0	0.0	7.260573264074776	0.0
6	0.0	0.0	0.0	8.248520538827718	0.0
7	9.646038613092568E-5	0.0	0.0	11.464509812432778	0.0
8	9.646038613092568E-5	0.0	0.0	15.268714520664226	0.0
9	9.646038613092568E-5	0.0	0.0	18.225997038666147	0.0
10	9.646038613092568E-5	0.0	0.0	20.032121308581598	0.0
11	9.646038613092568E-5	0.0	0.0	21.720949748961846	0.0
12	9.646038613092568E-5	0.0	0.0	22.961912616536203	0.0
13	9.646038613092568E-5	0.0	0.0	23.509228847443076	0.0
14	9.646038613092568E-5	0.0	0.0	23.907996083708323	0.0
15	9.646038613092568E-5	0.0	0.0	25.144328852748398	0.0
16	9.646038613092568E-5	0.0	0.0	26.681039264200173	0.0
17	9.646038613092568E-5	0.0	0.0	28.312473774832522	0.0
18	9.646038613092568E-5	0.0	0.0	29.24727137682732	0.0
19	9.646038613092568E-5	0.0	0.0	30.21611949512634	0.0
20	9.646038613092568E-5	0.0	0.0	31.27178196094319	0.0
21	9.646038613092568E-5	0.0	0.0	32.34500021703587	0.0
22	8.681434751783312E-4	0.0	0.0	33.477348689826805	0.0
23	8.681434751783312E-4	0.0	0.0	34.375684265864116	0.0
24	0.0012539850197020338	0.0	0.0	35.08408934160963	0.0
25	0.0013504454058329595	0.0	0.0	35.77165897395087	0.0
26	0.0013504454058329595	0.0	0.0	36.4304834112251	0.0
27	0.0013504454058329595	0.0	0.0	37.090079531588366	0.0
28	0.0013504454058329595	0.0	0.0	37.79443327111638	0.0
29	0.0013504454058329595	0.0	0.0	38.61945895369419	0.0
30	0.0013504454058329595	0.0	0.0	39.34387645353744	0.0
31	0.0013504454058329595	0.0	0.0	39.934985699747756	0.0
32	0.0014469057919638852	0.0	0.0	40.55223571059955	0.0
33	0.0014469057919638852	0.0	0.0	41.151833470789384	0.0
34	0.0014469057919638852	0.0	0.0	41.83525530652699	0.0
35	0.0014469057919638852	0.0	0.0	42.44247343722117	0.0
36	0.0014469057919638852	0.0	0.0	43.030881792619816	0.0
37	0.0014469057919638852	0.0	0.0	43.62748928083959	0.0
38	0.0017362869503566624	0.0	0.0	44.200078132912765	0.0
39	0.0017362869503566624	0.0	0.0	44.77459619270856	0.0
40	0.0017362869503566624	0.0	0.0	45.37409749251226	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	105	0.0	46.000004	1
CTAACGG	105	0.0	46.000004	1
ACCGGCG	60	0.0	46.0	19
AGATCCG	20	6.311168E-4	46.0	24
GACCGAC	20	6.311168E-4	46.0	8
ACTACGG	50	1.6370905E-11	46.0	1
AATTCGG	40	5.6097633E-9	46.0	1
TCATCGG	40	5.6097633E-9	46.0	1
TCGGTTG	20	6.311168E-4	46.0	28
AGTACGG	45	3.092282E-10	46.0	1
ACGGTGA	20	6.311168E-4	46.0	26
GCGAGTA	30	1.8612245E-6	46.0	21
TCACGAC	140	0.0	45.999996	24
CTCGTCC	35	1.0194344E-7	45.999996	36
CCGTGTA	35	1.0194344E-7	45.999996	24
CATCCGG	35	1.0194344E-7	45.999996	1
CGTTTTT	12445	0.0	45.519485	1
TTCACGG	225	0.0	44.97778	1
CGGTCTA	135	0.0	44.2963	30
ATCGCGG	110	0.0	43.909092	1
>>END_MODULE