FastQCFastQC Report
Thu 26 May 2016
SRR1043211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043211_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1010094
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505235.001811712573286No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC58010.5743029856627205No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT52000.5148035727367948No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC36240.35877848992272005No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG30150.2984870715002762No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC16830.16661815633000493No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC16650.1648361439628391No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC15290.15137205052203062No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG13990.13850196120361075No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG12940.1281068890618101No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG11670.11553380180458453No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT10560.10454472554039525No Hit
TCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG10370.10266371248616465No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT10170.10068369874486928No Hit
ACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC10110.10008969462248068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGA207.03288E-445.018
GCAACCG207.03288E-445.035
GGCAACG207.03288E-445.07
CAACCGG406.8139343E-945.01
GCACGGA207.03288E-445.02
TGTATCG253.890469E-545.011
CGTTATC207.03288E-445.016
CCGTAGA207.03288E-445.039
CGTAGAC207.03288E-445.040
GTCACGG551.8189894E-1245.01
CAGGCGA207.03288E-445.04
TTAACGG950.045.01
ACGTCCG207.03288E-445.039
TCATCGG406.8139343E-945.01
CGTAAGG207.03288E-445.01
ACGTATA207.03288E-445.025
TCTCACG950.045.022
CGGTCTA900.045.030
GCGGAAT207.03288E-445.04
CGACTCA253.890469E-545.021