##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043211_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1010094
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2527675642069	33.0	31.0	34.0	31.0	34.0
2	32.54640558205474	34.0	31.0	34.0	31.0	34.0
3	32.620451165931094	34.0	31.0	34.0	31.0	34.0
4	36.16845065904757	37.0	35.0	37.0	35.0	37.0
5	36.12636645698321	37.0	37.0	37.0	35.0	37.0
6	36.346928107681066	37.0	37.0	37.0	35.0	37.0
7	36.2910491498811	37.0	37.0	37.0	35.0	37.0
8	36.18850324821254	37.0	37.0	37.0	35.0	37.0
9	37.725254283264725	39.0	38.0	39.0	35.0	39.0
10	37.51925365362035	39.0	37.0	39.0	35.0	39.0
11	37.347883464311245	39.0	37.0	39.0	35.0	39.0
12	37.38268715584886	39.0	37.0	39.0	35.0	39.0
13	37.39836787467305	39.0	37.0	39.0	35.0	39.0
14	38.805566610632276	40.0	38.0	41.0	35.0	41.0
15	38.885610646137884	40.0	38.0	41.0	35.0	41.0
16	38.914501026637126	41.0	38.0	41.0	35.0	41.0
17	38.86762915134631	41.0	38.0	41.0	35.0	41.0
18	38.65508754630757	40.0	38.0	41.0	35.0	41.0
19	38.40910053915774	40.0	37.0	41.0	35.0	41.0
20	38.14292630190854	40.0	35.0	41.0	35.0	41.0
21	38.07946290147254	40.0	35.0	41.0	34.0	41.0
22	38.03807467423824	40.0	35.0	41.0	34.0	41.0
23	38.017127118862206	40.0	35.0	41.0	34.0	41.0
24	37.94550903183268	40.0	35.0	41.0	34.0	41.0
25	37.915372232683296	40.0	35.0	41.0	34.0	41.0
26	37.87120307614935	40.0	35.0	41.0	34.0	41.0
27	37.85969523628494	40.0	35.0	41.0	34.0	41.0
28	37.79302124356743	40.0	35.0	41.0	34.0	41.0
29	37.78511108867095	40.0	35.0	41.0	34.0	41.0
30	37.59294085500953	40.0	35.0	41.0	34.0	41.0
31	37.479736539371586	40.0	35.0	41.0	33.0	41.0
32	37.203241480495876	40.0	35.0	41.0	33.0	41.0
33	36.920533138499984	40.0	35.0	41.0	32.0	41.0
34	36.628652382847534	40.0	35.0	41.0	31.0	41.0
35	36.504358010244594	40.0	35.0	41.0	31.0	41.0
36	36.427756228628226	40.0	35.0	41.0	31.0	41.0
37	36.34219092480502	40.0	35.0	41.0	30.0	41.0
38	36.291024399709336	40.0	35.0	41.0	30.0	41.0
39	36.209884426597924	40.0	35.0	41.0	30.0	41.0
40	36.108213691003016	39.0	35.0	41.0	30.0	41.0
41	36.03051894180145	39.0	35.0	41.0	29.0	41.0
42	35.8963254904989	39.0	35.0	41.0	28.0	41.0
43	35.77681879112241	39.0	35.0	41.0	28.0	41.0
44	35.6631442222209	39.0	35.0	41.0	27.0	41.0
45	35.614113141945204	39.0	35.0	41.0	27.0	41.0
46	35.50257599787742	38.0	35.0	40.0	27.0	41.0
47	35.35042283193445	38.0	35.0	40.0	26.0	41.0
48	35.323303573726804	38.0	35.0	40.0	26.0	41.0
49	35.2248503604615	38.0	35.0	40.0	26.0	41.0
50	35.09034505699469	38.0	35.0	40.0	26.0	41.0
51	34.65406981924455	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	7.0
15	5.0
16	24.0
17	62.0
18	166.0
19	322.0
20	586.0
21	1005.0
22	1708.0
23	2779.0
24	4889.0
25	9496.0
26	14758.0
27	17308.0
28	16349.0
29	15301.0
30	15780.0
31	17756.0
32	21523.0
33	27453.0
34	50871.0
35	90554.0
36	87298.0
37	78051.0
38	142066.0
39	393903.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.63563985134057	19.290778878005412	25.908875807598104	28.16470546305591
2	32.78516652905571	20.609864032456386	26.187067738250104	20.4179017002378
3	21.908852047433207	21.768865075923628	35.980413704071104	20.341869172572057
4	22.842428526453972	24.384265226800675	33.833979807819865	18.939326438925484
5	20.93280427366166	30.43122719271672	30.056806594237763	18.57916193938386
6	76.71731541816901	1.4865943169645597	18.640542365364016	3.1555478995024226
7	77.2371680259461	1.174148148588151	15.922973505436127	5.665710320029621
8	67.41016182652308	3.343946206986677	22.1283365706558	7.117555395834447
9	32.413121947066315	26.8929426370219	25.52653515415397	15.167400261757816
10	23.362182133544007	20.303357905303862	43.416553310879976	12.917906650272155
11	21.787972208527126	17.809530598142352	40.48573697101458	19.916760222315943
12	17.93140044391908	21.775300120582834	42.316259674842144	17.977039760655938
13	14.8535680837625	25.119939332378966	40.68156033002869	19.344932253829842
14	13.37420081695367	23.05765602013278	44.011250438078044	19.55689272483551
15	14.390244868299387	20.310485954772524	45.22202884088016	20.077240336047932
16	14.922769564020774	23.857779572990236	38.67570740940942	22.543743453579566
17	15.266994952944973	22.086459280027405	41.496830988007055	21.149714779020567
18	17.127811867014355	26.81938512653278	39.132001576090936	16.920801430361927
19	17.359473474745915	23.79600314426182	36.82261254893109	22.021910832061174
20	15.142650089991625	28.259053117828635	36.20930329256485	20.388993499614887
21	15.549839915889017	22.918461054119714	37.19059810275083	24.341100927240436
22	13.408950058113403	25.14102647872376	35.2734497977416	26.176573665421238
23	12.81009490205862	22.367027227168958	43.26201323837188	21.56086463240055
24	16.859124002320577	22.485432048898417	38.80609131427372	21.849352634507284
25	14.969596889002409	25.584648557460987	38.067249186709354	21.37850536682725
26	16.252843794735934	23.20734505897471	37.144463782578654	23.3953473637107
27	19.835678659609897	23.45167875465056	38.2160472193677	18.496595366371842
28	16.441539104281382	23.190613942860764	35.22503846176692	25.14280849109093
29	20.51838739760854	22.522656307234772	34.0845505467808	22.874405748375896
30	19.936758361103028	25.005494538132094	32.605480282033156	22.452266818731722
31	23.961532293034114	21.932414210954622	31.315798331640423	22.79025516437084
32	19.4462099566971	24.440398616366398	30.834753993192717	25.27863743374379
33	19.154751933978424	23.86639263276487	34.21582545782867	22.76302997542803
34	18.648165418268004	22.783523117650436	31.645173617504906	26.923137846576655
35	20.171092987385332	21.512849299174135	32.83120184854083	25.484855864899703
36	17.234732609044308	25.354570960722466	32.74962528239946	24.661071147833766
37	16.50935457492075	22.589382770316426	35.26731175514358	25.633950899619247
38	15.910796420927161	24.138842523567114	31.1520511952353	28.798309860270432
39	20.352561246775053	22.703926565250363	33.09127665345997	23.85223553451461
40	16.265713884054357	22.01597079083729	36.28563282229179	25.43268250281657
41	20.42136672428507	23.79639914701008	30.141749183739336	25.640484944965518
42	20.165548948909706	21.309303886568973	33.858828980273124	24.666318184248198
43	22.281886636293255	22.564533597863168	33.28125897193727	21.87232079390631
44	17.343732365502618	23.90767591927088	33.87556009638707	24.873031618839434
45	19.710442790472964	21.567596679120953	33.64003746185998	25.081923068546097
46	22.010129750300468	20.994184699641817	31.87822123485537	25.117464315202348
47	17.882890107257342	20.30642692660287	35.25889669674307	26.55178626939671
48	18.821614622005477	18.82102061788309	32.59449120576897	29.762873554342466
49	19.8531027805333	18.76686724205866	36.090007464651805	25.290022512756238
50	18.78864739321291	18.0468352450366	36.510463382615875	26.654053979134616
51	16.911891368526096	17.934172463156894	35.526297552505014	29.627638615811996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	241.0
1	1229.0
2	2217.0
3	30131.5
4	58046.0
5	32904.5
6	7763.0
7	7299.0
8	6835.0
9	7083.5
10	7332.0
11	7416.0
12	7500.0
13	7240.0
14	6980.0
15	6448.5
16	5917.0
17	5598.5
18	5280.0
19	4984.0
20	4688.0
21	4695.5
22	4703.0
23	4485.5
24	4268.0
25	4822.0
26	6513.0
27	7650.0
28	8108.5
29	8567.0
30	10156.5
31	11746.0
32	13418.0
33	15090.0
34	18041.5
35	20993.0
36	22708.0
37	24423.0
38	25745.5
39	27068.0
40	31389.5
41	35711.0
42	41246.5
43	46782.0
44	51998.0
45	57214.0
46	63201.0
47	69188.0
48	84884.0
49	100580.0
50	118467.0
51	136354.0
52	133659.0
53	130964.0
54	104416.5
55	77869.0
56	59975.0
57	42081.0
58	33204.0
59	24327.0
60	19493.5
61	14660.0
62	11892.0
63	9124.0
64	7682.0
65	6240.0
66	5238.0
67	4236.0
68	3678.0
69	3120.0
70	2633.5
71	2147.0
72	2358.5
73	2570.0
74	2150.0
75	1534.5
76	1339.0
77	986.0
78	633.0
79	458.5
80	284.0
81	212.0
82	140.0
83	104.5
84	69.0
85	47.0
86	25.0
87	23.5
88	22.0
89	11.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1010094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.301704719638785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44820784831705	24.45950529951311
2	11.824816642885828	7.875731044105086
3	4.511823582084197	4.507542500330127
4	2.2298372305439775	2.9702952409773062
5	1.3403747355477316	2.231838182843724
6	0.886426273426788	1.7711703608033214
7	0.6163795650143398	1.436854318852886
8	0.462786145432831	1.232925405083516
9	0.3640742632665183	1.0911864250189462
>10	3.8025573627610214	27.70675804122425
>50	0.380712322698752	8.393597176610305
>100	0.12150370761024576	6.900157263385563
>500	0.006000183091824912	1.3277794835304164
>1k	0.0036001098550949473	1.9479094499919132
>5k	6.000183091824913E-4	1.0990896988952679
>10k+	3.0000915459124563E-4	5.047660108834253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50523	5.001811712573286	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	5801	0.5743029856627205	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	5200	0.5148035727367948	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	3624	0.35877848992272005	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	3015	0.2984870715002762	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1683	0.16661815633000493	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	1665	0.1648361439628391	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1529	0.15137205052203062	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	1399	0.13850196120361075	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	1294	0.1281068890618101	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	1167	0.11553380180458453	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	1056	0.10454472554039525	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	1037	0.10266371248616465	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	1017	0.10068369874486928	No Hit
ACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1011	0.10008969462248068	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5268816565586966	0.0
2	0.0	0.0	0.0	1.7588462063926724	0.0
3	0.0	0.0	0.0	2.962001556290801	0.0
4	0.0	0.0	0.0	3.7292568810427547	0.0
5	0.0	0.0	0.0	7.323971828364489	0.0
6	0.0	0.0	0.0	8.294970567095735	0.0
7	0.0	0.0	0.0	11.53674806503157	0.0
8	0.0	0.0	0.0	15.377677721083383	0.0
9	0.0	0.0	0.0	18.445808013907616	0.0
10	0.0	0.0	0.0	20.311475961643175	0.0
11	0.0	0.0	0.0	22.055571065663194	0.0
12	0.0	0.0	0.0	23.350500052470363	0.0
13	0.0	0.0	0.0	23.914605967365414	0.0
14	0.0	0.0	0.0	24.31694475959663	0.0
15	0.0	0.0	0.0	25.58138153478785	0.0
16	0.0	0.0	0.0	27.147968406900745	0.0
17	0.0	0.0	0.0	28.8242480402814	0.0
18	0.0	0.0	0.0	29.77762465671512	0.0
19	0.0	0.0	0.0	30.764265504002598	0.0
20	0.0	0.0	0.0	31.84436299987922	0.0
21	0.0	0.0	0.0	32.931885547285695	0.0
22	2.970020611943047E-4	0.0	0.0	34.092371601058915	0.0
23	2.970020611943047E-4	0.0	0.0	35.012384985951805	0.0
24	6.930048094533776E-4	0.0	0.0	35.7376640193883	0.0
25	6.930048094533776E-4	0.0	0.0	36.45304298411831	0.0
26	6.930048094533776E-4	0.0	0.0	37.09634944866517	0.0
27	6.930048094533776E-4	0.0	0.0	37.76836611246082	0.0
28	7.920054965181459E-4	0.0	0.0	38.46622195558037	0.0
29	7.920054965181459E-4	0.0	0.0	39.30723279219558	0.0
30	7.920054965181459E-4	0.0	0.0	40.02766079196589	0.0
31	7.920054965181459E-4	0.0	0.0	40.62295192328635	0.0
32	7.920054965181459E-4	0.0	0.0	41.24843826416156	0.0
33	7.920054965181459E-4	0.0	0.0	41.87778563183228	0.0
34	7.920054965181459E-4	0.0	0.0	42.581581516175724	0.0
35	7.920054965181459E-4	0.0	0.0	43.20627585155441	0.0
36	7.920054965181459E-4	0.0	0.0	43.80473500486093	0.0
37	7.920054965181459E-4	0.0	0.0	44.396561112134115	0.0
38	9.900068706476823E-4	0.0	0.0	44.969379087490864	0.0
39	9.900068706476823E-4	0.0	0.0	45.56457121812426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTGA	20	7.03288E-4	45.0	18
GCAACCG	20	7.03288E-4	45.0	35
GGCAACG	20	7.03288E-4	45.0	7
CAACCGG	40	6.8139343E-9	45.0	1
GCACGGA	20	7.03288E-4	45.0	2
TGTATCG	25	3.890469E-5	45.0	11
CGTTATC	20	7.03288E-4	45.0	16
CCGTAGA	20	7.03288E-4	45.0	39
CGTAGAC	20	7.03288E-4	45.0	40
GTCACGG	55	1.8189894E-12	45.0	1
CAGGCGA	20	7.03288E-4	45.0	4
TTAACGG	95	0.0	45.0	1
ACGTCCG	20	7.03288E-4	45.0	39
TCATCGG	40	6.8139343E-9	45.0	1
CGTAAGG	20	7.03288E-4	45.0	1
ACGTATA	20	7.03288E-4	45.0	25
TCTCACG	95	0.0	45.0	22
CGGTCTA	90	0.0	45.0	30
GCGGAAT	20	7.03288E-4	45.0	4
CGACTCA	25	3.890469E-5	45.0	21
>>END_MODULE