FastQCFastQC Report
Thu 26 May 2016
SRR1043210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043210_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences726493
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438606.037222657341503No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT42740.5883057372885905No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT39260.54040438104703Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT33230.45740289307673987No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC19310.265797468110498No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT15760.21693257884109002No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG13430.18486069377130956No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC12840.17673948682230936No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC11290.155404112634258No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC10040.13819816570841012No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT9220.1269110645250539No Hit
TCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC9040.12443340816773182No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT8860.12195575181040974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG351.0188705E-746.0000041
GCGATAT351.0188705E-746.0000048
CGTTTTC700.046.0000041
TTCTCCG351.0188705E-746.00000446
TCACGAC650.046.024
ACAACGC206.3099124E-446.034
CGGGTCG206.3099124E-446.05
GCGACCG206.3099124E-446.014
CTCCGTT206.3099124E-446.021
CGACGGT650.046.027
GCAACGC206.3099124E-446.012
CATGAGT301.8604496E-646.034
ATGGACG301.8604496E-646.011
CGGTTGA253.4159057E-546.026
CTATAAG206.3099124E-446.033
TCGGGTA253.4159057E-546.04
CGACCGT206.3099124E-446.015
CGCGATG206.3099124E-446.046
TAACGTA206.3099124E-446.028
CGACATC253.4159057E-546.010
Produced by FastQC (version 0.11.5)