##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043210_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	726493
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.225713117676285	33.0	31.0	34.0	31.0	34.0
2	32.55722216181023	34.0	31.0	34.0	31.0	34.0
3	32.640139684759525	34.0	31.0	34.0	31.0	34.0
4	36.163704261431285	37.0	35.0	37.0	35.0	37.0
5	36.059269669494405	37.0	35.0	37.0	35.0	37.0
6	36.30366844553217	37.0	37.0	37.0	35.0	37.0
7	36.230214193392094	37.0	37.0	37.0	35.0	37.0
8	36.20338255151805	37.0	37.0	37.0	35.0	37.0
9	37.717876152970504	39.0	38.0	39.0	35.0	39.0
10	37.517663625114075	39.0	37.0	39.0	35.0	39.0
11	37.105139347522964	39.0	37.0	39.0	34.0	39.0
12	37.07511565837524	39.0	37.0	39.0	33.0	39.0
13	37.09472906139495	39.0	37.0	39.0	33.0	39.0
14	38.476227575489375	40.0	37.0	41.0	34.0	41.0
15	38.591306454432456	40.0	37.0	41.0	35.0	41.0
16	38.61203342633721	40.0	37.0	41.0	35.0	41.0
17	38.59858525821997	40.0	37.0	41.0	35.0	41.0
18	38.31111104993441	40.0	37.0	41.0	34.0	41.0
19	38.02117157357332	40.0	37.0	41.0	34.0	41.0
20	37.71913562828548	39.0	35.0	41.0	34.0	41.0
21	37.6258518664323	39.0	35.0	41.0	34.0	41.0
22	37.577148024826116	39.0	35.0	41.0	34.0	41.0
23	37.55634259380338	39.0	35.0	41.0	34.0	41.0
24	37.478848385325115	39.0	35.0	41.0	34.0	41.0
25	37.369333221379975	39.0	35.0	41.0	33.0	41.0
26	37.328484926902256	39.0	35.0	41.0	33.0	41.0
27	37.335602683026536	39.0	35.0	41.0	33.0	41.0
28	37.3398683813884	39.0	35.0	41.0	33.0	41.0
29	37.30301599602474	39.0	35.0	41.0	33.0	41.0
30	37.26869082014555	39.0	35.0	41.0	33.0	41.0
31	37.14128973025205	39.0	35.0	41.0	33.0	41.0
32	36.95702367400649	39.0	35.0	41.0	33.0	41.0
33	36.76201973040346	39.0	35.0	41.0	32.0	41.0
34	36.55237559068016	39.0	35.0	41.0	31.0	41.0
35	36.41377549405156	39.0	35.0	41.0	31.0	41.0
36	36.323504837624036	39.0	35.0	41.0	31.0	41.0
37	36.26481053499483	39.0	35.0	41.0	31.0	41.0
38	36.22452659557628	39.0	35.0	41.0	31.0	41.0
39	36.18410363210657	39.0	35.0	41.0	30.0	41.0
40	36.143006195517366	39.0	35.0	41.0	30.0	41.0
41	36.120854571207154	39.0	35.0	41.0	30.0	41.0
42	36.03290327642524	39.0	35.0	41.0	30.0	41.0
43	35.92397173819982	39.0	35.0	41.0	30.0	41.0
44	35.76750223333191	38.0	35.0	41.0	30.0	41.0
45	35.68974511798462	38.0	35.0	41.0	29.0	41.0
46	35.64935656640876	38.0	35.0	41.0	28.0	41.0
47	35.57088918957237	38.0	35.0	41.0	28.0	41.0
48	35.52929209228444	38.0	35.0	40.0	28.0	41.0
49	35.462195781652404	38.0	35.0	40.0	28.0	41.0
50	35.318955585256845	38.0	35.0	40.0	27.0	41.0
51	35.22605035423604	38.0	35.0	40.0	27.0	41.0
52	34.89371817760116	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	4.0
15	7.0
16	19.0
17	44.0
18	145.0
19	257.0
20	511.0
21	812.0
22	1342.0
23	2287.0
24	4244.0
25	7448.0
26	10578.0
27	11404.0
28	10786.0
29	10496.0
30	11422.0
31	13725.0
32	16914.0
33	22376.0
34	41907.0
35	81090.0
36	70273.0
37	53762.0
38	87964.0
39	265847.0
40	825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.953735273430024	19.771973026581126	25.242775911123715	28.031515788865136
2	33.305895583302245	20.497513396550275	26.711475540714087	19.485115479433386
3	20.74940845954469	21.759053425153443	37.541311478568964	19.950226636732907
4	22.552866992524358	25.020888019567977	34.614511082694534	17.811733905213124
5	20.209279373648474	29.647223028989956	31.193693538685164	18.94980405867641
6	74.0047047941274	1.785013757875162	20.55092065580811	3.6593607921893256
7	74.8895034088422	1.52664925883663	17.24008352454876	6.343763807772408
8	63.79442059317846	3.361353791433641	24.699618578568547	8.14460703681935
9	36.637104555721805	19.027850233932053	27.827935024838506	16.50711018550764
10	28.999315891550225	18.787379919696402	40.66659967817997	11.546704510573399
11	25.691369359374423	16.203046691434054	39.04029357474883	19.0652903744427
12	18.36301244471729	22.97957447628539	40.809615509027616	17.847797569969703
13	13.612656969853804	25.343258641170664	40.705278646869274	20.338805742106256
14	11.744504076432946	22.21411630944827	45.45425764597869	20.587121968140092
15	12.804252759489767	18.105473831131203	48.185873779926304	20.904399629452726
16	13.65945714549211	24.453091771014996	37.38136499594628	24.50608608754661
17	15.780330987359822	21.265586867320124	41.190899292904405	21.763182852415646
18	17.892395384401503	26.8700455475827	37.78329591613409	17.45426315188171
19	18.096664386305168	22.770074866516264	36.089955443479845	23.043305303698727
20	14.602067741877761	29.512741347817528	35.30343719760548	20.58175371269923
21	15.94949985753476	23.523557694292993	35.65994441790905	24.866998030263197
22	13.357045422323408	26.44939455713957	32.84078442600273	27.35277559453429
23	12.807418653724124	22.374338087221762	44.54468246769067	20.27356079136344
24	16.498576035832414	21.967038911593093	39.473745789704786	22.060639262869707
25	13.177277688842151	26.157168754550973	37.764713493454174	22.900840063152707
26	14.690850428015137	23.543241297576163	36.55162541139419	25.21428286301451
27	18.979811230115086	22.37571456297583	38.40491236667112	20.239561840237965
28	13.303225220339357	21.90124337054865	36.6532093220444	28.142322087067594
29	15.594782055711478	23.51846473400294	33.93453206018503	26.952221150100552
30	20.020013957464148	22.212051595817165	30.483019106859942	27.284915339858745
31	18.629360503129416	20.551884188835956	33.85359528584584	26.96516002218879
32	15.602490319934258	25.26356069501014	29.836901387900504	29.2970475971551
33	13.209624869062745	21.057188438154256	33.101075991096955	32.632110701686045
34	16.965476611612225	22.804624407943365	34.41161855654494	25.818280423899477
35	17.2389823439455	23.09987845719092	30.97001622864914	28.69112297021444
36	17.153227904467077	27.259863481134712	30.178680317635543	25.40822829676267
37	18.085652580272622	22.88569882985796	34.369360750895055	24.65928783897436
38	15.932569205759725	25.910641946997426	30.665677439424744	27.49111140781811
39	20.714996565692996	24.229965051280605	31.426455588698033	23.62858279432837
40	15.20372529398081	20.57693604755999	36.36814119337695	27.85119746508225
41	18.091984368741336	22.877852918059773	30.72101176473827	28.30915094846062
42	17.725428875433074	20.816993419069423	35.82443327052015	25.63314443497735
43	20.240938315992032	22.957275569069488	33.67465343781702	23.12713267712146
44	15.331324596382897	25.69522349148581	33.0025203271057	25.970931585025596
45	18.857167240427643	21.540193780256658	33.024956881897005	26.57768209741869
46	21.715419143749493	20.48966748475209	32.14593946534929	25.64897390614913
47	15.217627699096894	19.608172411847054	37.23958799327729	27.93461189577876
48	17.160247930812822	18.069410166374624	33.47575269135422	31.294589211458334
49	18.915254517249306	18.534521323674145	37.096159219703424	25.45406493937313
50	17.080137041926076	18.1217162450292	36.93662567980696	27.861521033237764
51	15.438276762473969	17.110419508515566	36.50840407271646	30.942899656294003
52	15.360230587218322	17.275321303852895	42.793530013365576	24.570918095563204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	209.0
1	1228.5
2	2248.0
3	25425.0
4	48602.0
5	26876.5
6	5151.0
7	4887.5
8	4624.0
9	4843.0
10	5062.0
11	5215.5
12	5369.0
13	5286.0
14	4846.0
15	4489.0
16	4117.0
17	3745.0
18	3435.0
19	3125.0
20	2959.0
21	2793.0
22	2609.5
23	2426.0
24	2370.5
25	2315.0
26	2779.0
27	3243.0
28	3051.5
29	2860.0
30	3417.5
31	3975.0
32	5222.0
33	6469.0
34	7569.5
35	8670.0
36	10403.0
37	12136.0
38	12752.0
39	16108.5
40	18849.0
41	22047.0
42	25245.0
43	32264.5
44	39284.0
45	44551.0
46	49818.0
47	58216.0
48	66614.0
49	82426.0
50	98238.0
51	99952.5
52	101667.0
53	87989.0
54	74311.0
55	59508.5
56	44706.0
57	34716.5
58	24727.0
59	19257.0
60	13787.0
61	11012.5
62	8238.0
63	6698.0
64	4096.0
65	3034.0
66	2430.5
67	1827.0
68	1557.0
69	1287.0
70	1186.5
71	1086.0
72	1188.0
73	1290.0
74	961.5
75	633.0
76	485.5
77	338.0
78	231.0
79	124.0
80	103.5
81	83.0
82	57.0
83	31.0
84	30.0
85	29.0
86	16.5
87	4.0
88	3.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	726493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.15517650845737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41020005971097	24.670833170093427
2	11.562768867989243	7.667312854893585
3	4.481375254684732	4.457423627091163
4	2.1323781144470337	2.827974910690517
5	1.2447732369351758	2.06353381917948
6	0.8178903161880626	1.6270378678663937
7	0.5895881474416969	1.3683529367006697
8	0.41893253264888514	1.1111825652087097
9	0.3282491511852549	0.9794842691668592
>10	3.417126519537618	25.20018537727704
>50	0.4490977165537439	9.891314762396005
>100	0.13514514611331263	7.627496343720303
>500	0.008316624376203854	1.7324690740942326
>1k	0.003742480969291734	2.7284388808152844
>5k	0.0	0.0
>10k+	4.158312188101927E-4	6.046959540806386
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43860	6.037222657341503	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	4274	0.5883057372885905	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	3926	0.54040438104703	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3323	0.45740289307673987	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1931	0.265797468110498	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1576	0.21693257884109002	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	1343	0.18486069377130956	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1284	0.17673948682230936	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1129	0.155404112634258	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1004	0.13819816570841012	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	922	0.1269110645250539	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	904	0.12443340816773182	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	886	0.12195575181040974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.555683261917183	0.0
2	0.0	0.0	0.0	1.973315641031641	0.0
3	0.0	0.0	0.0	3.343872549356979	0.0
4	0.0	0.0	0.0	4.217797005614645	0.0
5	0.0	0.0	0.0	8.714330351428025	0.0
6	0.0	0.0	0.0	9.890804178429798	0.0
7	0.0	0.0	0.0	13.613895798032466	0.0
8	0.0	0.0	0.0	17.997420484436876	0.0
9	0.0	0.0	0.0	21.064070816924595	0.0
10	0.0	0.0	0.0	23.06147478365242	0.0
11	0.0	0.0	0.0	24.97973139452135	0.0
12	0.0	0.0	0.0	26.326475272301316	0.0
13	0.0	0.0	0.0	26.88257147694472	0.0
14	0.0	0.0	0.0	27.316987224928525	0.0
15	0.0	0.0	0.0	28.581555500190643	0.0
16	0.0	0.0	0.0	30.10737887357483	0.0
17	0.0	0.0	0.0	31.7667203951036	0.0
18	0.0	0.0	0.0	32.67478145006215	0.0
19	0.0	0.0	0.0	33.635561526401496	0.0
20	0.0	0.0	0.0	34.749681001743994	0.0
21	0.001514123329474613	0.0	0.0	35.83916156108868	0.0
22	0.0017894184802881789	0.0	0.0	36.90551732776503	0.0
23	0.002064713631101745	0.0	0.0	37.74420400471856	0.0
24	0.002340008781915311	0.0	0.0	38.46299964349278	0.0
25	0.002340008781915311	0.0	0.0	39.140913952371186	0.0
26	0.002340008781915311	0.0	0.0	39.81910355640041	0.0
27	0.002615303932728877	0.0	0.0	40.44939180418807	0.0
28	0.002615303932728877	0.0	0.0	41.134876729713845	0.0
29	0.002615303932728877	0.0	0.0	41.89771959261824	0.0
30	0.002615303932728877	0.0	0.0	42.56627386636898	0.0
31	0.002615303932728877	0.0	0.0	43.15223959487565	0.0
32	0.002615303932728877	0.0	0.0	43.75761363151469	0.0
33	0.002615303932728877	0.0	0.0	44.33779816185428	0.0
34	0.002615303932728877	0.0	0.0	45.066779721208604	0.0
35	0.002615303932728877	0.0	0.0	45.60924881588673	0.0
36	0.002615303932728877	0.0	0.0	46.14786377845348	0.0
37	0.002615303932728877	0.0	0.0	46.68689168374644	0.0
38	0.002615303932728877	0.0	0.0	47.20885128968896	0.0
39	0.002615303932728877	0.0	0.0	47.74677801437866	0.0
40	0.002615303932728877	0.0	0.0	48.26433289790817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	35	1.0188705E-7	46.000004	1
GCGATAT	35	1.0188705E-7	46.000004	8
CGTTTTC	70	0.0	46.000004	1
TTCTCCG	35	1.0188705E-7	46.000004	46
TCACGAC	65	0.0	46.0	24
ACAACGC	20	6.3099124E-4	46.0	34
CGGGTCG	20	6.3099124E-4	46.0	5
GCGACCG	20	6.3099124E-4	46.0	14
CTCCGTT	20	6.3099124E-4	46.0	21
CGACGGT	65	0.0	46.0	27
GCAACGC	20	6.3099124E-4	46.0	12
CATGAGT	30	1.8604496E-6	46.0	34
ATGGACG	30	1.8604496E-6	46.0	11
CGGTTGA	25	3.4159057E-5	46.0	26
CTATAAG	20	6.3099124E-4	46.0	33
TCGGGTA	25	3.4159057E-5	46.0	4
CGACCGT	20	6.3099124E-4	46.0	15
CGCGATG	20	6.3099124E-4	46.0	46
TAACGTA	20	6.3099124E-4	46.0	28
CGACATC	25	3.4159057E-5	46.0	10
>>END_MODULE