FastQCFastQC Report
Thu 26 May 2016
SRR1043209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043209_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences709014
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404465.704541800302955No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC41380.5836274036901951No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT37850.5338399523845792No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC33600.47389755350388No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG18930.266990496661561No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC16090.2269348701153997No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG12830.1809555241504399No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC12760.17996823758064015No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG12080.1703774537597283No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG9310.13130911378336677No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC9120.12862933595105316No Hit
TCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG8800.12411602591768287No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC8220.11593565148219921No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT7150.10084427105811733Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCC453.8380676E-1045.0000044
CGGGTAT1200.045.0000045
ATTAGTC600.045.00000443
CAAGTCG302.164321E-645.00000440
GGCGTAC302.164321E-645.0000047
TCTACGG302.164321E-645.0000041
CTCGCGG453.8380676E-1045.0000041
GCGATAT453.8380676E-1045.0000048
CTCTATT302.164321E-645.00000424
TGTGTCC302.164321E-645.00000411
TTTAGTG253.8892886E-545.012
GTGTTCG207.031456E-445.08
CGGGTTC351.2110468E-745.05
CTTCGAA253.8892886E-545.023
CGGAATC207.031456E-445.05
AGCGTAT207.031456E-445.039
AATCCGG253.8892886E-545.01
GCCTTAC253.8892886E-545.019
ATCTAGT351.2110468E-745.011
CGCGGAT253.8892886E-545.022