##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043209_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	709014
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17635899996333	33.0	31.0	34.0	30.0	34.0
2	32.473003353953516	34.0	31.0	34.0	31.0	34.0
3	32.543646528841464	34.0	31.0	34.0	31.0	34.0
4	36.11567895697405	37.0	35.0	37.0	35.0	37.0
5	36.07159096999495	37.0	35.0	37.0	35.0	37.0
6	36.30519143486588	37.0	37.0	37.0	35.0	37.0
7	36.24943654145052	37.0	37.0	37.0	35.0	37.0
8	36.148724848874636	37.0	37.0	37.0	35.0	37.0
9	37.671969805955875	39.0	38.0	39.0	35.0	39.0
10	37.46248875198515	39.0	37.0	39.0	35.0	39.0
11	37.09925756049951	39.0	35.0	39.0	34.0	39.0
12	37.06550223267806	39.0	37.0	39.0	33.0	39.0
13	37.09284301861458	39.0	37.0	39.0	33.0	39.0
14	38.47523744242004	40.0	37.0	41.0	34.0	41.0
15	38.602886543848214	40.0	38.0	41.0	35.0	41.0
16	38.65378398734016	40.0	38.0	41.0	35.0	41.0
17	38.62450247808929	40.0	37.0	41.0	35.0	41.0
18	38.37564702530557	40.0	37.0	41.0	35.0	41.0
19	38.10468763663341	40.0	37.0	41.0	34.0	41.0
20	37.83120643598011	40.0	35.0	41.0	34.0	41.0
21	37.735851478250076	39.0	35.0	41.0	34.0	41.0
22	37.7059310535476	39.0	35.0	41.0	34.0	41.0
23	37.66026481846621	39.0	35.0	41.0	34.0	41.0
24	37.57606478856553	39.0	35.0	41.0	34.0	41.0
25	37.531566372455266	39.0	35.0	41.0	34.0	41.0
26	37.52669199761923	39.0	35.0	41.0	34.0	41.0
27	37.51220145159334	39.0	35.0	41.0	34.0	41.0
28	37.4649231185844	39.0	35.0	41.0	34.0	41.0
29	37.450274606707346	39.0	35.0	41.0	34.0	41.0
30	37.36939468049996	39.0	35.0	41.0	33.0	41.0
31	37.23400102113639	39.0	35.0	41.0	33.0	41.0
32	37.066826607090974	39.0	35.0	41.0	33.0	41.0
33	36.84380844383891	39.0	35.0	41.0	33.0	41.0
34	36.58217891325136	39.0	35.0	41.0	32.0	41.0
35	36.43583483541933	39.0	35.0	41.0	31.0	41.0
36	36.32337020143467	39.0	35.0	41.0	31.0	41.0
37	36.225298795228305	39.0	35.0	41.0	31.0	41.0
38	36.177040227696494	39.0	35.0	41.0	30.0	41.0
39	36.08848767443238	39.0	35.0	41.0	30.0	41.0
40	36.0235876865619	39.0	35.0	41.0	30.0	41.0
41	35.97034755308076	39.0	35.0	41.0	30.0	41.0
42	35.86867255089462	39.0	35.0	41.0	30.0	41.0
43	35.743045976525146	38.0	35.0	41.0	29.0	41.0
44	35.64781654523042	38.0	35.0	40.0	29.0	41.0
45	35.59977800156273	38.0	35.0	40.0	28.0	41.0
46	35.49735266158355	38.0	35.0	40.0	28.0	41.0
47	35.36812108082492	38.0	35.0	40.0	27.0	41.0
48	35.36246816000813	38.0	35.0	40.0	27.0	41.0
49	35.27398330639451	38.0	35.0	40.0	27.0	41.0
50	35.15469229098438	38.0	35.0	40.0	26.0	41.0
51	34.751134674350574	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	3.0
15	9.0
16	13.0
17	47.0
18	105.0
19	228.0
20	443.0
21	722.0
22	1124.0
23	1980.0
24	3405.0
25	6246.0
26	9800.0
27	11700.0
28	10989.0
29	10712.0
30	11450.0
31	13427.0
32	16392.0
33	21670.0
34	42094.0
35	79092.0
36	65840.0
37	52884.0
38	92324.0
39	256220.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.39184275627844	19.80257089422776	25.337722527340784	27.467863822153017
2	32.86676990863368	20.519764066718004	26.947422758930006	19.666043265718308
3	20.863057711131233	21.80267244370351	37.139746182726995	20.19452366243826
4	22.71407898856722	25.14082937713501	34.19819636847791	17.946895265819858
5	20.386339338856494	29.7944751443554	30.687123244392918	19.132062272395185
6	74.51460761000487	1.7460868191601295	20.06899722713515	3.6703083436998423
7	75.43334828367281	1.4710569890016276	16.699811287224232	6.395783440101324
8	64.02214906898877	3.302050453164536	24.33125438989921	8.344546087947489
9	36.93001830711382	18.98269991847834	27.379713235563756	16.707568538844082
10	29.296036467545072	18.713875889615718	40.287497849125685	11.702589793713523
11	25.86493355561385	16.14961058596868	38.768204859142415	19.21725099927505
12	18.664793643002817	22.772610978062495	40.62472673318157	17.937868645753117
13	13.602270194946787	25.48130220277738	40.52275413461511	20.393673467660722
14	11.910484137125641	22.10661566626329	45.21363470961081	20.769265487000258
15	12.95305875483418	18.01741573509127	47.95152140860406	21.078004101470494
16	13.673213787033825	24.455370415816898	37.27768422062188	24.593731576527404
17	16.007300278978974	21.23836764859368	40.81992739212484	21.934404680302507
18	18.039418121503946	27.03049587173173	37.595731537035945	17.334354469728382
19	18.297805120914397	22.744261749415383	35.76854617821369	23.18938695145653
20	14.673899245995143	29.68601466261597	34.80960883706105	20.830477254327842
21	15.92944568090334	23.48726541365897	35.41396925871704	25.169319646720655
22	13.450933267890338	26.508080235369118	32.36085606208058	27.680130434659965
23	12.949814813247693	22.337922805473518	44.180509834784644	20.531752546494143
24	16.614622560344365	21.84286911118821	39.277926811036174	22.26458151743125
25	13.163350794201525	26.158862871537092	37.533814565015646	23.14397176924574
26	14.791527388739855	23.28642311717399	36.388139021232305	25.533910472853847
27	19.26026848553061	22.166699106082532	38.09515750041607	20.47787490797079
28	13.375476365769929	21.938917990335874	36.26092009466667	28.42468554922752
29	15.741861232641385	23.499959098127825	33.49200438919401	27.26617528003678
30	20.210320247555057	22.17657197178053	29.900537930139603	27.712569850524815
31	18.696809936052038	20.521879680796147	33.41485499581108	27.36645538734073
32	15.752016180216469	25.14449644153712	29.296882713176327	29.806604665070086
33	13.185353180614204	20.870109758058376	32.58863717782724	33.355899883500186
34	17.11729246531098	22.556254178337802	34.12767589920651	26.19877745714471
35	17.25353801194335	22.965837063866157	30.773440298781125	29.007184625409373
36	17.243806187183893	27.336131585554025	29.805617378500283	25.614444848761803
37	18.343220303125186	22.614222004078904	34.08592778139783	24.956629911398082
38	16.17062568581156	25.792156431325758	30.17486255560539	27.862355327257287
39	20.919474086548362	24.047480021551053	31.13365885581949	23.899387036081094
40	15.283196100500133	20.30566392201	36.16106311017836	28.250076867311506
41	18.172842849365455	22.748069854756043	30.422530443686586	28.656556852191915
42	17.961704564366855	20.527662359276402	35.58702649030908	25.923606586047665
43	20.511442651343977	22.793061914151203	33.411470013286056	23.28402542121876
44	15.500681227733162	25.43405348836553	32.89638850572768	26.168876778173633
45	19.067465522542573	21.219327121890398	32.858025370444025	26.855181985123
46	21.901824223499116	20.278866143686866	31.898664906475755	25.920644726338267
47	15.340176639671432	19.40173254688906	37.14129763305097	28.116793180388537
48	17.36919158154846	17.73053846609517	33.168033353361146	31.73223659899522
49	19.122471488574273	18.209513493386588	36.910836739471996	25.75717827856714
50	17.28033579026648	17.796686666271754	36.86415218881432	28.05882535464744
51	15.773595443813521	16.91630912788746	36.08024665239332	31.229848775905694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	1160.0
2	2110.0
3	23567.5
4	45025.0
5	25140.0
6	5255.0
7	4933.0
8	4611.0
9	4811.0
10	5011.0
11	4993.0
12	4975.0
13	4883.0
14	4791.0
15	4426.0
16	4061.0
17	3647.5
18	3234.0
19	2955.5
20	2677.0
21	2523.5
22	2370.0
23	2239.0
24	2108.0
25	2049.0
26	2586.5
27	3183.0
28	3065.0
29	2947.0
30	3730.0
31	4513.0
32	5491.5
33	6470.0
34	8162.0
35	9854.0
36	11116.0
37	12378.0
38	13657.0
39	14936.0
40	18319.0
41	21702.0
42	26533.5
43	31365.0
44	36246.5
45	41128.0
46	45330.5
47	49533.0
48	62151.5
49	74770.0
50	90834.5
51	106899.0
52	103882.0
53	100865.0
54	81432.5
55	62000.0
56	47229.0
57	32458.0
58	25260.5
59	18063.0
60	13948.5
61	9834.0
62	8161.5
63	6489.0
64	5101.5
65	3714.0
66	2914.0
67	2114.0
68	1776.0
69	1438.0
70	1286.5
71	1135.0
72	1143.0
73	1151.0
74	979.0
75	619.5
76	432.0
77	318.5
78	205.0
79	154.5
80	104.0
81	78.5
82	53.0
83	42.0
84	31.0
85	18.5
86	6.0
87	6.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	709014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.716012307194994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.81933054386917	24.15074126582119
2	11.777776180059277	7.706437409164148
3	4.495477309411829	4.412222729442997
4	2.183900385233193	2.8579404752390807
5	1.3200246151524142	2.1592970777563365
6	0.8596604822435705	1.6874797750253905
7	0.5883224226695379	1.3473294534458764
8	0.4416397851620028	1.155895411736563
9	0.3509873788013069	1.0334616664080303
>10	3.580012668805294	26.078536592531222
>50	0.426075806463461	9.20618095325252
>100	0.14419690899397003	7.984876019394957
>500	0.008686560782769278	1.8362912908609645
>1k	0.0034746243131077107	2.636143003021946
>5k	0.0	0.0
>10k+	4.3432803913846384E-4	5.747166876898752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40446	5.704541800302955	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	4138	0.5836274036901951	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3785	0.5338399523845792	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	3360	0.47389755350388	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	1893	0.266990496661561	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1609	0.2269348701153997	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	1283	0.1809555241504399	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	1276	0.17996823758064015	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	1208	0.1703774537597283	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	931	0.13130911378336677	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	912	0.12862933595105316	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	880	0.12411602591768287	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	822	0.11593565148219921	No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	715	0.10084427105811733	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5478030053003184	0.0
2	0.0	0.0	0.0	1.9978448944590657	0.0
3	0.0	0.0	0.0	3.402189519529939	0.0
4	0.0	0.0	0.0	4.298504683969569	0.0
5	0.0	0.0	0.0	8.895875116711377	0.0
6	0.0	0.0	0.0	10.074554240113736	0.0
7	0.0	0.0	0.0	13.877300025105287	0.0
8	0.0	0.0	0.0	18.40640664359237	0.0
9	0.0	0.0	0.0	21.622845247061413	0.0
10	0.0	0.0	0.0	23.735638506432878	0.0
11	0.0	0.0	0.0	25.690183832759296	0.0
12	0.0	0.0	0.0	27.018366351017047	0.0
13	0.0	0.0	0.0	27.60805851506458	0.0
14	0.0	0.0	0.0	28.0448623017317	0.0
15	0.0	0.0	0.0	29.353863252347626	0.0
16	0.0	0.0	0.0	30.961024747043076	0.0
17	0.0	0.0	0.0	32.6639530390091	0.0
18	0.0	0.0	0.0	33.61654353792732	0.0
19	1.4104093854282144E-4	0.0	0.0	34.632320377312716	0.0
20	1.4104093854282144E-4	0.0	0.0	35.7939335471514	0.0
21	4.231228156284643E-4	0.0	0.0	36.94482760566082	0.0
22	0.0011283275083425715	0.0	0.0	38.05030648195945	0.0
23	0.001269368446885393	0.0	0.0	38.91686200836655	0.0
24	0.002538736893770786	0.0	0.0	39.63024707551614	0.0
25	0.002538736893770786	0.0	0.0	40.30710253958314	0.0
26	0.002538736893770786	0.0	0.0	40.99368982840959	0.0
27	0.002538736893770786	0.0	0.0	41.635144016902345	0.0
28	0.00296185970939925	0.0	0.0	42.318487364142314	0.0
29	0.00296185970939925	0.0	0.0	43.082365087290235	0.0
30	0.00296185970939925	0.0	0.0	43.75724597821764	0.0
31	0.00296185970939925	0.0	0.0	44.346938142265174	0.0
32	0.00296185970939925	0.0	0.0	44.95073440016699	0.0
33	0.00296185970939925	0.0	0.0	45.56101854124178	0.0
34	0.00296185970939925	0.0	0.0	46.29936785451345	0.0
35	0.00296185970939925	0.0	0.0	46.870019491857704	0.0
36	0.00296185970939925	0.0	0.0	47.42106643874451	0.0
37	0.00296185970939925	0.0	0.0	47.938686683196664	0.0
38	0.00296185970939925	0.0	0.0	48.45574276389465	0.0
39	0.00296185970939925	0.0	0.0	48.99339082161988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCC	45	3.8380676E-10	45.000004	4
CGGGTAT	120	0.0	45.000004	5
ATTAGTC	60	0.0	45.000004	43
CAAGTCG	30	2.164321E-6	45.000004	40
GGCGTAC	30	2.164321E-6	45.000004	7
TCTACGG	30	2.164321E-6	45.000004	1
CTCGCGG	45	3.8380676E-10	45.000004	1
GCGATAT	45	3.8380676E-10	45.000004	8
CTCTATT	30	2.164321E-6	45.000004	24
TGTGTCC	30	2.164321E-6	45.000004	11
TTTAGTG	25	3.8892886E-5	45.0	12
GTGTTCG	20	7.031456E-4	45.0	8
CGGGTTC	35	1.2110468E-7	45.0	5
CTTCGAA	25	3.8892886E-5	45.0	23
CGGAATC	20	7.031456E-4	45.0	5
AGCGTAT	20	7.031456E-4	45.0	39
AATCCGG	25	3.8892886E-5	45.0	1
GCCTTAC	25	3.8892886E-5	45.0	19
ATCTAGT	35	1.2110468E-7	45.0	11
CGCGGAT	25	3.8892886E-5	45.0	22
>>END_MODULE