FastQCFastQC Report
Thu 26 May 2016
SRR1043208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043208_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1209808
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671445.54997156573605No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT91580.7569796199066299No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT88640.7326782431592451Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT60640.5012365598508193No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC39390.32558885376853186No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT33060.2732665017920199No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG32410.26789374842950286No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC22060.18234298334942403No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC20890.17267202729689338No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT20000.165315488077447No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC18710.15465263909645166No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC17260.14266726621083675No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT15960.1319217594858027No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC13130.10852961792284395No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG12830.10604988560168226No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT12750.10538862364937246No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT12220.10100776321532012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA351.0196345E-746.00000439
GACACCG351.0196345E-746.00000431
GGGCGCA351.0196345E-746.0000046
TCGGTTA351.0196345E-746.00000435
CGATAGT351.0196345E-746.0000049
TGATCCG206.311587E-446.010
AATCCGG453.092282E-1046.01
GTACCGG600.046.01
TCCATCG206.311587E-446.017
ACCGGCC206.311587E-446.020
GATCGAT206.311587E-446.08
CAACGCA650.046.016
GTTTAGT206.311587E-446.020
CCGGATG206.311587E-446.011
CAACCGG600.046.01
CGACCCC253.417264E-546.019
CGATTAC253.417264E-546.09
GCACCGG253.417264E-546.01
TATTGTC253.417264E-546.028
AATGCGT206.311587E-446.01
Produced by FastQC (version 0.11.5)