##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043208_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1209808
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27892442437147	33.0	31.0	34.0	31.0	34.0
2	32.59623014560988	34.0	31.0	34.0	31.0	34.0
3	32.67168261410075	34.0	31.0	34.0	31.0	34.0
4	36.185692275137875	37.0	35.0	37.0	35.0	37.0
5	36.08269246029122	37.0	35.0	37.0	35.0	37.0
6	36.31512934283787	37.0	37.0	37.0	35.0	37.0
7	36.233230396889425	37.0	37.0	37.0	35.0	37.0
8	36.219608400671845	37.0	37.0	37.0	35.0	37.0
9	37.76325912872125	39.0	38.0	39.0	35.0	39.0
10	37.58993658497877	39.0	37.0	39.0	35.0	39.0
11	37.25285334532422	39.0	37.0	39.0	34.0	39.0
12	37.27486262282941	39.0	37.0	39.0	34.0	39.0
13	37.2459067885152	39.0	37.0	39.0	34.0	39.0
14	38.66044612037613	40.0	38.0	41.0	34.0	41.0
15	38.765210678058004	41.0	38.0	41.0	35.0	41.0
16	38.7719398450002	41.0	38.0	41.0	35.0	41.0
17	38.71473903297052	40.0	38.0	41.0	35.0	41.0
18	38.456231897954055	40.0	38.0	41.0	35.0	41.0
19	38.17606843399945	40.0	37.0	41.0	34.0	41.0
20	37.89440225225821	40.0	35.0	41.0	34.0	41.0
21	37.785718890931456	40.0	35.0	41.0	34.0	41.0
22	37.77081983256847	40.0	35.0	41.0	34.0	41.0
23	37.72218814886329	39.0	35.0	41.0	34.0	41.0
24	37.637031661222274	39.0	35.0	41.0	34.0	41.0
25	37.53856810336847	39.0	35.0	41.0	34.0	41.0
26	37.46132609471916	39.0	35.0	41.0	33.0	41.0
27	37.4600845754037	39.0	35.0	41.0	33.0	41.0
28	37.47875200031741	39.0	35.0	41.0	33.0	41.0
29	37.47818744792562	39.0	35.0	41.0	33.0	41.0
30	37.332017973099866	39.0	35.0	41.0	33.0	41.0
31	37.212903204475424	39.0	35.0	41.0	33.0	41.0
32	36.96398684749977	39.0	35.0	41.0	33.0	41.0
33	36.7264656871173	39.0	35.0	41.0	32.0	41.0
34	36.406043768928626	39.0	35.0	41.0	31.0	41.0
35	36.30648416938886	39.0	35.0	41.0	30.0	41.0
36	36.238033638395514	39.0	35.0	41.0	30.0	41.0
37	36.17415821353471	39.0	35.0	41.0	30.0	41.0
38	36.133126082816446	39.0	35.0	41.0	30.0	41.0
39	36.15399303029902	39.0	35.0	41.0	30.0	41.0
40	36.14140921534657	39.0	35.0	41.0	30.0	41.0
41	36.12361300305503	39.0	35.0	41.0	30.0	41.0
42	36.07127907899435	39.0	35.0	41.0	30.0	41.0
43	35.97788657373732	39.0	35.0	41.0	30.0	41.0
44	35.83233537883697	39.0	35.0	41.0	29.0	41.0
45	35.717540303915996	39.0	35.0	41.0	28.0	41.0
46	35.679121811064235	39.0	35.0	41.0	28.0	41.0
47	35.59484232208747	38.0	35.0	41.0	27.0	41.0
48	35.54410369248674	38.0	35.0	41.0	27.0	41.0
49	35.49432223956198	38.0	35.0	40.0	27.0	41.0
50	35.356592120402574	38.0	35.0	40.0	26.0	41.0
51	35.274560922063664	38.0	35.0	40.0	26.0	41.0
52	34.9456517067171	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	5.0
13	3.0
14	5.0
15	11.0
16	54.0
17	103.0
18	223.0
19	441.0
20	802.0
21	1385.0
22	2177.0
23	3840.0
24	7273.0
25	13055.0
26	18379.0
27	19879.0
28	18526.0
29	17307.0
30	18943.0
31	22357.0
32	27586.0
33	36460.0
34	64965.0
35	115730.0
36	113554.0
37	91397.0
38	153931.0
39	460797.0
40	619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.999986774760952	19.76726885588457	25.85418512689617	27.378559242458305
2	33.4787007525161	19.113198127306152	27.4244342903998	19.98366682977795
3	20.841240924179704	21.588962876753996	38.83194688743999	18.73784931162631
4	22.379088252020154	24.009016306719744	36.36915940380622	17.242736037453877
5	20.038964860539853	29.23687064393689	31.79562376839961	18.928540727123643
6	72.60681033684683	1.6618339438985361	21.70832065914591	4.023035060108712
7	73.76393609564494	1.5082558554745877	17.850766402602726	6.877041646277757
8	61.30261991985505	3.541305727851031	25.934776427333922	9.221297924959995
9	35.29204634123762	16.902103474270298	29.201575787232354	18.60427439725973
10	27.304828534775766	19.654110404295558	41.20910094824964	11.831960112679036
11	24.007859098303204	16.462529591472368	40.504443680319525	19.02516762990491
12	19.506731646674513	19.83843717350191	42.45053760596723	18.204293573856347
13	14.287721688069514	23.126892862338487	42.019477470805285	20.565907978786715
14	12.754172562919075	22.734103262666473	43.00045957705685	21.511264597357595
15	13.958413235819236	18.201813841535184	46.926867734384295	20.912905188261277
16	14.72159218652877	25.24458426461058	37.909899752688034	22.123923796172615
17	16.28332760239641	21.835200296245354	40.41566926322193	21.465802838136298
18	18.069313477841114	27.373021173607714	38.67679830187931	15.880867046671868
19	17.181238675889066	23.28972861809477	36.1281294221893	23.400903283826853
20	14.49775501567191	29.424917011624984	35.125160356023436	20.95216761667967
21	15.18092127015196	23.725996191131156	37.04885403303665	24.04422850568024
22	13.339141417481121	26.82409109544655	32.75858648645074	27.078181000621587
23	13.371212622168146	23.189134143599645	43.17528070569875	20.264372528533453
24	17.901104968722308	21.744194120058722	38.26061656064434	22.094084350574636
25	14.686627960800392	25.37237313689445	37.877828548001006	22.063170354304155
26	16.843499133746842	21.771719148823614	37.18424741777208	24.200534299657466
27	21.497047465382938	21.52085289566609	38.65274489836404	18.329354740586936
28	15.734728155211405	21.484152857312896	36.484384298996204	26.296734688479496
29	22.008616243238595	21.61012325922791	34.5173779641067	21.863882533426793
30	21.9437299141682	20.23668218428048	36.51306653617764	21.30652136537368
31	21.92504926401545	20.157330750003304	32.93101054051552	24.986609445465728
32	19.46226178038168	20.716841019401425	39.82673283694603	19.994164363270865
33	20.61608122941822	18.799511988679196	38.05512940896406	22.529277372938516
34	16.022211788978087	17.880109901736475	43.78273246663934	22.314945842646104
35	20.45886620025657	22.658802057847197	37.32063269543597	19.561699046460266
36	19.4093608241969	20.202709851480567	41.91367555843572	18.474253765886818
37	17.444255617420286	23.347919669898033	41.949879650324675	17.257945062357003
38	15.954349781122293	24.24318569558145	38.515698358747834	21.286766164548425
39	22.199059685503816	21.257174692182563	39.73275098197399	16.811014640339625
40	16.466166532210067	19.11766164548424	43.39845661460331	21.017715207702377
41	18.956396386864693	22.471251636623332	34.79105775461891	23.78129422189306
42	18.790336979090895	18.04864904183143	40.572801634639546	22.588212344438123
43	23.19921838837237	20.12724333117321	36.269308849007444	20.404229431446975
44	17.089488580006083	22.52894674196236	37.537774589025695	22.843790089005857
45	19.50119357782392	20.61608122941822	37.080098660283284	22.802626532474573
46	22.736830968219753	19.144608070040867	36.5580323489347	21.560528612804678
47	16.505263645140385	18.121718487561665	41.11173012577202	24.261287741525926
48	18.157922579450624	15.8905380027244	36.477854337217146	29.47368508060783
49	20.05739753746049	16.60999100683745	40.35458519037732	22.978026265324747
50	18.45276275243675	16.34490762170526	40.755309933477044	24.447019692380938
51	16.463273511168712	15.45336119450359	39.50469826617116	28.578667028156534
52	15.068754711491412	16.021715842513853	46.811890812426434	22.0976386335683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	334.0
1	1869.5
2	3405.0
3	40011.5
4	76618.0
5	43031.0
6	9444.0
7	9070.5
8	8697.0
9	9114.0
10	9531.0
11	9729.5
12	9928.0
13	9669.0
14	8663.0
15	7916.0
16	7169.0
17	6422.0
18	5902.5
19	5383.0
20	4925.0
21	4467.0
22	4124.5
23	3782.0
24	3542.5
25	3303.0
26	3774.0
27	4245.0
28	4401.0
29	4557.0
30	5459.0
31	6361.0
32	8021.5
33	9682.0
34	11635.0
35	13588.0
36	15948.5
37	18309.0
38	21554.0
39	29452.0
40	34105.0
41	47522.5
42	60940.0
43	86302.0
44	111664.0
45	145268.5
46	178873.0
47	182628.5
48	186384.0
49	162464.5
50	138545.0
51	113149.0
52	87753.0
53	71691.0
54	55629.0
55	45890.5
56	36152.0
57	31836.5
58	27521.0
59	23449.5
60	19378.0
61	15924.0
62	12470.0
63	9873.0
64	5778.5
65	4281.0
66	3528.0
67	2775.0
68	2366.0
69	1957.0
70	1670.5
71	1384.0
72	1268.5
73	1153.0
74	934.0
75	715.0
76	510.5
77	306.0
78	281.0
79	256.0
80	166.0
81	76.0
82	45.5
83	15.0
84	11.0
85	7.0
86	5.5
87	4.0
88	3.0
89	3.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1209808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.799206246708266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.07337165450726	22.19802638482826
2	12.28451041677736	7.56706339932324
3	5.094982415570875	4.7076424272155775
4	2.6047638450538217	3.2089863555112603
5	1.5432533784040592	2.3765489546197966
6	0.966324007833055	1.7857207451037596
7	0.6860115586622728	1.4790028038005605
8	0.49685882758786865	1.2242286005101144
9	0.3887591379335187	1.0776125582556255
>10	3.1963234239493987	21.44917468776
>50	0.4611880073824395	9.589741101669338
>100	0.18695159852017035	10.758924062543091
>500	0.010236542856142748	2.1080442221103937
>1k	0.005387654134811973	2.900137192375189
>5k	8.081481202217959E-4	1.9983608758560665
>10k+	2.6938270674059864E-4	5.5707856285178
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67144	5.54997156573605	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	9158	0.7569796199066299	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	8864	0.7326782431592451	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	6064	0.5012365598508193	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3939	0.32558885376853186	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	3306	0.2732665017920199	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	3241	0.26789374842950286	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2206	0.18234298334942403	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2089	0.17267202729689338	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	2000	0.165315488077447	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1871	0.15465263909645166	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1726	0.14266726621083675	No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	1596	0.1319217594858027	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1313	0.10852961792284395	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1283	0.10604988560168226	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	1275	0.10538862364937246	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT	1222	0.10100776321532012	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.75631835795432	0.0
2	0.0	0.0	0.0	2.4658458201631994	0.0
3	0.0	0.0	0.0	4.026589343102376	0.0
4	0.0	0.0	0.0	4.9785585811963555	0.0
5	0.0	0.0	0.0	10.037129858622194	0.0
6	0.0	0.0	0.0	11.28716292171981	0.0
7	0.0	0.0	0.0	15.186376681258514	0.0
8	0.0	0.0	0.0	20.01325830214381	0.0
9	0.0	0.0	0.0	23.498687395024664	0.0
10	0.0	0.0	0.0	25.814096121037387	0.0
11	0.0	0.0	0.0	27.85367595519289	0.0
12	0.0	0.0	0.0	29.22455464007512	0.0
13	0.0	0.0	0.0	29.840850779627843	0.0
14	0.0	0.0	0.0	30.269844471188815	0.0
15	0.0	0.0	0.0	31.664528586354198	0.0
16	0.0	0.0	0.0	33.29495258751802	0.0
17	0.0	0.0	0.0	35.0044800497269	0.0
18	0.0	0.0	0.0	35.976783092854404	0.0
19	0.0	0.0	0.0	37.00636795260074	0.0
20	0.0	0.0	0.0	38.171015566106355	0.0
21	0.0	0.0	0.0	39.36054316056763	0.0
22	1.65315488077447E-4	0.0	0.0	40.46377607025247	0.0
23	2.479732321161705E-4	0.0	0.0	41.31539880708344	0.0
24	0.00165315488077447	0.0	0.0	42.07890838876913	0.0
25	0.00165315488077447	0.0	0.0	42.810842712232024	0.0
26	0.00165315488077447	0.0	0.0	43.584353880946395	0.0
27	0.00165315488077447	0.0	0.0	44.31132873976697	0.0
28	0.00165315488077447	0.0	0.0	45.01987092166691	0.0
29	0.00165315488077447	0.0	0.0	45.841736870643935	0.0
30	0.00165315488077447	0.0	0.0	46.546476796318096	0.0
31	0.00165315488077447	0.0	0.0	47.203523203681904	0.0
32	0.00165315488077447	0.0	0.0	47.84337680028567	0.0
33	0.00165315488077447	0.0	0.0	48.48397431658577	0.0
34	0.00165315488077447	0.0	0.0	49.20276605874651	0.0
35	0.00165315488077447	0.0	0.0	49.79087590758203	0.0
36	0.00165315488077447	0.0	0.0	50.356502850039014	0.0
37	0.00165315488077447	0.0	0.0	50.94742306217185	0.0
38	0.00165315488077447	0.0	0.0	51.53288786319813	0.0
39	0.00165315488077447	0.0	0.0	52.107276526523215	0.0
40	0.00165315488077447	0.0	0.0	52.66728274238557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGA	35	1.0196345E-7	46.000004	39
GACACCG	35	1.0196345E-7	46.000004	31
GGGCGCA	35	1.0196345E-7	46.000004	6
TCGGTTA	35	1.0196345E-7	46.000004	35
CGATAGT	35	1.0196345E-7	46.000004	9
TGATCCG	20	6.311587E-4	46.0	10
AATCCGG	45	3.092282E-10	46.0	1
GTACCGG	60	0.0	46.0	1
TCCATCG	20	6.311587E-4	46.0	17
ACCGGCC	20	6.311587E-4	46.0	20
GATCGAT	20	6.311587E-4	46.0	8
CAACGCA	65	0.0	46.0	16
GTTTAGT	20	6.311587E-4	46.0	20
CCGGATG	20	6.311587E-4	46.0	11
CAACCGG	60	0.0	46.0	1
CGACCCC	25	3.417264E-5	46.0	19
CGATTAC	25	3.417264E-5	46.0	9
GCACCGG	25	3.417264E-5	46.0	1
TATTGTC	25	3.417264E-5	46.0	28
AATGCGT	20	6.311587E-4	46.0	1
>>END_MODULE