##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043207_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1200533
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.213734233044825	33.0	31.0	34.0	30.0	34.0
2	32.50249264285113	34.0	31.0	34.0	31.0	34.0
3	32.56511399520046	34.0	31.0	34.0	31.0	34.0
4	36.13230206916428	37.0	35.0	37.0	35.0	37.0
5	36.08537041464083	37.0	35.0	37.0	35.0	37.0
6	36.31503673784894	37.0	37.0	37.0	35.0	37.0
7	36.24495619862178	37.0	37.0	37.0	35.0	37.0
8	36.15390997165426	37.0	37.0	37.0	35.0	37.0
9	37.70639374344562	39.0	38.0	39.0	35.0	39.0
10	37.522778632490734	39.0	37.0	39.0	35.0	39.0
11	37.240743902916456	39.0	37.0	39.0	34.0	39.0
12	37.270465701484255	39.0	37.0	39.0	34.0	39.0
13	37.254067151840054	39.0	37.0	39.0	34.0	39.0
14	38.656475915280964	40.0	38.0	41.0	35.0	41.0
15	38.77408617672317	40.0	38.0	41.0	35.0	41.0
16	38.81025594465125	40.0	38.0	41.0	35.0	41.0
17	38.74551636648056	40.0	38.0	41.0	35.0	41.0
18	38.510324997313695	40.0	38.0	41.0	35.0	41.0
19	38.255777225615624	40.0	37.0	41.0	35.0	41.0
20	37.99841986850841	40.0	35.0	41.0	34.0	41.0
21	37.89057860133791	40.0	35.0	41.0	34.0	41.0
22	37.886580377215786	40.0	35.0	41.0	34.0	41.0
23	37.80939965831843	40.0	35.0	41.0	34.0	41.0
24	37.7235686149402	39.0	35.0	41.0	34.0	41.0
25	37.69552273865025	39.0	35.0	41.0	34.0	41.0
26	37.659743630537434	39.0	35.0	41.0	34.0	41.0
27	37.622289433110126	39.0	35.0	41.0	34.0	41.0
28	37.58668191544922	39.0	35.0	41.0	34.0	41.0
29	37.61827954750098	40.0	35.0	41.0	34.0	41.0
30	37.4358039304209	39.0	35.0	41.0	33.0	41.0
31	37.30959332229934	39.0	35.0	41.0	33.0	41.0
32	37.07860925105766	39.0	35.0	41.0	33.0	41.0
33	36.81705125973214	39.0	35.0	41.0	32.0	41.0
34	36.474026120065005	39.0	35.0	41.0	31.0	41.0
35	36.37155663359525	39.0	35.0	41.0	31.0	41.0
36	36.29159798189637	39.0	35.0	41.0	30.0	41.0
37	36.18664126683731	39.0	35.0	41.0	30.0	41.0
38	36.12783322074445	39.0	35.0	41.0	30.0	41.0
39	36.091379412311035	39.0	35.0	41.0	30.0	41.0
40	36.05214600514938	39.0	35.0	41.0	30.0	41.0
41	35.99407846348247	39.0	35.0	41.0	30.0	41.0
42	35.925272358194235	39.0	35.0	41.0	29.0	41.0
43	35.81016681757186	39.0	35.0	41.0	29.0	41.0
44	35.702257247405946	39.0	35.0	40.0	28.0	41.0
45	35.63038250510398	38.0	35.0	40.0	28.0	41.0
46	35.52710587713957	38.0	35.0	40.0	27.0	41.0
47	35.38297406235397	38.0	35.0	40.0	26.0	41.0
48	35.356084339206	38.0	35.0	40.0	26.0	41.0
49	35.299414510055115	38.0	35.0	40.0	26.0	41.0
50	35.200665870909006	38.0	35.0	40.0	26.0	41.0
51	34.76968646426212	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	5.0
15	16.0
16	32.0
17	98.0
18	190.0
19	364.0
20	707.0
21	1145.0
22	1885.0
23	3239.0
24	5656.0
25	11092.0
26	17365.0
27	20503.0
28	19474.0
29	18392.0
30	19686.0
31	22463.0
32	27232.0
33	36605.0
34	65321.0
35	113471.0
36	108173.0
37	92534.0
38	165420.0
39	449388.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.301040454531446	19.805453077924557	26.136807567971893	26.756698899572108
2	33.19292347648919	19.23853821594242	27.457887454988743	20.110650852579646
3	20.998090015018327	21.75367107776296	38.35946200562583	18.888776901592877
4	22.596463404171313	24.27713357317125	35.868068599530375	17.25833442312706
5	20.191281705709045	29.417017274827096	31.26544626428428	19.126254755179577
6	73.07470931661187	1.6370228890001357	21.273384405093402	4.014883389294589
7	74.25285269126296	1.4548538024360846	17.335550126485487	6.956743379815465
8	61.44004371391707	3.5085249634953812	25.591466456982026	9.459964865605528
9	35.55095944884481	16.847183709235814	28.782299195440693	18.81955764647869
10	27.574335732545464	19.671012791818303	40.906830549430964	11.847820926205276
11	24.169931188896932	16.34540658190987	40.28918821889944	19.19547401029376
12	19.747812013497338	19.73515097044396	42.162606109119864	18.354430906938834
13	14.33813147993433	23.044264505848652	41.82300694774737	20.794597066469645
14	12.778657479636129	22.788794643712418	42.64747408026268	21.785073796388772
15	14.021355514592267	18.11620338632924	46.871931050625015	20.99051004845348
16	14.82516515580996	25.221630725686005	37.72057910944556	22.232625009058477
17	16.359067180993776	21.776660866465143	40.177571128823615	21.686700823717466
18	18.117952609382666	27.516528075446487	38.47899224761002	15.886527067560824
19	17.19952720999756	23.329054678213758	35.88681027510281	23.584607836685873
20	14.632833916268856	29.355711171621273	34.780968119993375	21.2304867921165
21	15.157934017640498	23.73870605805921	36.79590648486964	24.307453439430653
22	13.427869121465216	26.796181362778032	32.40035884061496	27.375590675141794
23	13.474265180548972	23.265666166610995	42.844719803620556	20.415348849219473
24	18.039320868314324	21.703859868908225	37.919574055856856	22.337245206920592
25	14.715047399779932	25.292515907517743	37.79937744318565	22.193059249516672
26	16.928980711067503	21.5426814589853	37.00631302929616	24.522024800651042
27	21.825972297304613	21.5082800722679	38.300904681503965	18.36484294892352
28	15.877281174278423	21.52943734158078	36.08263996075077	26.510641523390028
29	22.229626340966888	21.504198551809907	34.046627622897496	22.219547484325712
30	22.066032337303515	20.05076078708374	36.1960895702159	21.687117305396853
31	22.127421736845218	20.01560973334344	32.43509341267587	25.42187511713547
32	19.71599281319214	20.47099080158563	39.562344392032536	20.25067199318969
33	20.934368318072057	18.610483843426213	37.710750141812014	22.74439769668972
34	16.194723510307504	17.585189245110296	43.7038382118609	22.5162490327213
35	20.588521931508755	22.439449811042262	37.293268906394076	19.678759351054907
36	19.67775979502438	19.84993332128313	41.87639989904484	18.59590698464765
37	17.669901618697693	23.065505071497412	41.851494294617474	17.413099015187424
38	16.090769683132407	24.010002224012165	38.42701533402247	21.47221275883295
39	22.39055486188218	21.05006692860588	39.735350881650064	16.824027327861874
40	16.52399392603119	18.87744859991354	43.57706118865538	21.021496285399902
41	19.16115591991224	22.232291823714966	34.73873687770349	23.867815378669306
42	19.011139218996895	17.749283026788934	40.51800325355488	22.72157450065929
43	23.340549572564854	19.947556626931537	36.13453357800244	20.577360222501174
44	17.313726486485585	22.303843376233722	37.46044465250018	22.92198548478051
45	19.724239150444014	20.322889916395468	37.037715747921965	22.915155185238557
46	23.08766189684082	18.905352872432495	36.37109517189449	21.635890058832203
47	16.66743021641221	17.84174195961294	41.06975818240731	24.421069641567534
48	18.32844245014506	15.647299990920699	36.24081970258211	29.783437856352137
49	20.24284213761721	16.204302588933412	40.37340081447157	23.179454458977805
50	18.661960978998497	15.912348931682843	40.82636628897332	24.599323800345346
51	16.695917563282308	15.28154578008268	39.184428916156406	28.838107740478602
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	279.0
1	1821.0
2	3363.0
3	37678.0
4	71993.0
5	40964.0
6	9935.0
7	9336.5
8	8738.0
9	9056.0
10	9374.0
11	9464.5
12	9555.0
13	9020.5
14	8486.0
15	7836.0
16	7186.0
17	6521.0
18	5856.0
19	5331.0
20	4806.0
21	4374.5
22	3943.0
23	3607.5
24	3272.0
25	3281.0
26	3598.0
27	3906.0
28	4232.5
29	4559.0
30	6045.5
31	7532.0
32	8722.5
33	9913.0
34	12953.0
35	15993.0
36	18198.0
37	20403.0
38	24381.0
39	28359.0
40	35713.5
41	43068.0
42	56603.0
43	70138.0
44	101527.0
45	132916.0
46	164105.0
47	195294.0
48	191398.5
49	187503.0
50	152212.5
51	116922.0
52	94735.0
53	72548.0
54	58919.5
55	45291.0
56	38798.0
57	32305.0
58	28040.0
59	23775.0
60	19673.5
61	15572.0
62	12215.5
63	8859.0
64	7333.5
65	5808.0
66	4421.0
67	3034.0
68	2691.5
69	2349.0
70	1933.5
71	1518.0
72	1367.0
73	1216.0
74	1005.0
75	630.0
76	466.0
77	361.5
78	257.0
79	176.5
80	96.0
81	63.0
82	30.0
83	19.0
84	8.0
85	11.5
86	15.0
87	9.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1200533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.38157449107936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63607123614804	21.764166345092995
2	12.379133091923219	7.521951083345016
3	5.043107944267506	4.59652679025952
4	2.70333878909446	3.2852675518199046
5	1.6441113547855168	2.497534579852279
6	0.9829863391535743	1.7918803612024625
7	0.6905853711748768	1.468674962775945
8	0.5132005753286628	1.2473473206570045
9	0.4018488256265608	1.0987920026933506
>10	3.3337735334644742	21.950753368989844
>50	0.4629981935980627	9.48101780340343
>100	0.19199351597727554	11.080809787633381
>500	0.010497487203193722	2.1378394171676125
>1k	0.005248743601596861	2.8326036561481986
>5k	8.287489897258201E-4	1.9682189137875974
>10k+	2.7624966324194005E-4	5.276616055171474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62870	5.236840636617235	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	8814	0.7341739044241182	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	8551	0.7122669680883408	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	6086	0.5069415001503499	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	3932	0.32752119267025565	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3167	0.2637994957239826	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	3021	0.2516382306858704	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	2354	0.19607957465559048	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	2178	0.1814194195411538	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1901	0.15834633450309155	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	1892	0.1575966674801942	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1691	0.1408541039688205	No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1574	0.1311084326711552	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTC	1321	0.11003445969415251	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1311	0.1092014963353777	No Hit
TGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	1273	0.10603623557203343	Illumina Single End Adapter 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1214	0.10112175175526203	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	1202	0.10012219572473227	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7376723505309725	0.0
2	0.0	0.0	0.0	2.4255060044163717	0.0
3	0.0	0.0	0.0	4.030792989447187	0.0
4	0.0	0.0	0.0	5.016438531885421	0.0
5	0.0	0.0	0.0	10.168983276594647	0.0
6	0.0	0.0	0.0	11.415096461321763	0.0
7	0.0	0.0	0.0	15.403491615807312	0.0
8	0.0	0.0	0.0	20.417764442959918	0.0
9	0.0	0.0	0.0	24.02974345561513	0.0
10	0.0	0.0	0.0	26.41301821774162	0.0
11	0.0	0.0	0.0	28.48509786902984	0.0
12	0.0	0.0	0.0	29.903134691008077	0.0
13	0.0	0.0	0.0	30.5359369546693	0.0
14	0.0	0.0	0.0	30.988236058484024	0.0
15	0.0	0.0	0.0	32.433843967637706	0.0
16	0.0	0.0	0.0	34.12809143938567	0.0
17	0.0	0.0	0.0	35.90896710044622	0.0
18	0.0	0.0	0.0	36.9240162494492	0.0
19	0.0	0.0	0.0	37.95489170226891	0.0
20	0.0	0.0	0.0	39.15627475462982	0.0
21	0.0	0.0	0.0	40.3566582509602	0.0
22	6.663706870198487E-4	0.0	0.0	41.4904046785886	0.0
23	7.496670228973297E-4	0.0	0.0	42.36168435186705	0.0
24	0.002582186412201914	0.0	0.0	43.15316613537487	0.0
25	0.002582186412201914	0.0	0.0	43.90341623262334	0.0
26	0.002582186412201914	0.0	0.0	44.68623519719991	0.0
27	0.002582186412201914	0.0	0.0	45.40699839154775	0.0
28	0.002582186412201914	0.0	0.0	46.114767357498714	0.0
29	0.002582186412201914	0.0	0.0	46.93098815276215	0.0
30	0.002582186412201914	0.0	0.0	47.64133930512531	0.0
31	0.002582186412201914	0.0	0.0	48.330533188175586	0.0
32	0.002582186412201914	0.0	0.0	48.99098983534813	0.0
33	0.002582186412201914	0.0	0.0	49.66185852450536	0.0
34	0.002582186412201914	0.0	0.0	50.39020168541806	0.0
35	0.002582186412201914	0.0	0.0	50.97294285121692	0.0
36	0.002582186412201914	0.0	0.0	51.54502208602346	0.0
37	0.002582186412201914	0.0	0.0	52.14783766876879	0.0
38	0.002582186412201914	0.0	0.0	52.74199043258286	0.0
39	0.002582186412201914	0.0	0.0	53.32089996693136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGG	35	1.2119926E-7	45.000004	1
TCATCGG	70	0.0	45.000004	1
AATCGGT	70	0.0	45.000004	12
CGAACTC	20	7.0334127E-4	45.0	30
GATCGCC	20	7.0334127E-4	45.0	8
CGACGGC	25	3.89091E-5	45.0	2
CATGCGA	20	7.0334127E-4	45.0	23
CGCGGAA	20	7.0334127E-4	45.0	3
TTAGCGA	20	7.0334127E-4	45.0	39
GCGAACT	20	7.0334127E-4	45.0	29
GACACCG	45	3.8562575E-10	45.0	31
CGGCCAC	20	7.0334127E-4	45.0	22
GGCGTAT	20	7.0334127E-4	45.0	7
TTCGTCC	20	7.0334127E-4	45.0	43
GTTAGCG	20	7.0334127E-4	45.0	38
TGCGGTA	20	7.0334127E-4	45.0	3
TACGGAA	20	7.0334127E-4	45.0	3
CCGTCGC	40	6.8157533E-9	45.0	29
CCCGTCG	40	6.8157533E-9	45.0	28
ACGGCTC	40	6.8157533E-9	45.0	4
>>END_MODULE