FastQCFastQC Report
Thu 26 May 2016
SRR1043206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043206_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1303789
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555994.264417018397915No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT69030.5294568369575138Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT69010.5293034379029121No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT53110.4073511894946191No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC29780.22841119230182183No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT29160.22365582160917144No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG22370.1715768425719192No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC21340.16367679125993548No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC18640.14296791888871588No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC17080.13100279262978903No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC15310.11742697629754507No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT15290.11727357724294345No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT14090.10806963396684587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAT206.3117675E-446.00000419
ACGGATA206.3117675E-446.0000044
GTCGTAG206.3117675E-446.00000412
TGTACGA206.3117675E-446.00000412
CCACGTC206.3117675E-446.00000437
CCTGCGT206.3117675E-446.00000422
CGGAGAT206.3117675E-446.0000045
TCGCGAG206.3117675E-446.0000041
GCGATCG501.6370905E-1146.08
CGATCGG253.4174096E-546.09
TATCCGG551.8189894E-1246.01
CGTTTTT139300.045.471641
CAACGCA1150.044.016
GCCACGG1050.043.8095251
CCCGCAC1050.043.80952532
CTAGCGG3000.043.6999971
GTCACGG900.043.4444471
TCACGAC1650.043.2121224
GGCGATA3200.043.1250047
CGGTCTA1550.043.0322630
Produced by FastQC (version 0.11.5)