##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043206_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1303789
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24569773176488	33.0	31.0	34.0	31.0	34.0
2	32.579212587312824	34.0	31.0	34.0	31.0	34.0
3	32.68073284864345	34.0	31.0	34.0	31.0	34.0
4	36.182373835030056	37.0	35.0	37.0	35.0	37.0
5	36.07915621316026	37.0	35.0	37.0	35.0	37.0
6	36.323496363291916	37.0	37.0	37.0	35.0	37.0
7	36.25249330988373	37.0	37.0	37.0	35.0	37.0
8	36.21892882974162	37.0	37.0	37.0	35.0	37.0
9	37.74104552193645	39.0	38.0	39.0	35.0	39.0
10	37.51118240758282	39.0	37.0	39.0	35.0	39.0
11	37.281012495119995	39.0	37.0	39.0	34.0	39.0
12	37.30657491357881	39.0	37.0	39.0	34.0	39.0
13	37.30681421610399	39.0	37.0	39.0	34.0	39.0
14	38.68265493879761	40.0	38.0	41.0	35.0	41.0
15	38.76190549237645	40.0	38.0	41.0	35.0	41.0
16	38.78361452658367	40.0	38.0	41.0	35.0	41.0
17	38.75101109151864	40.0	38.0	41.0	35.0	41.0
18	38.525275945724346	40.0	38.0	41.0	35.0	41.0
19	38.29233948131178	40.0	37.0	41.0	34.0	41.0
20	37.996013158570904	40.0	35.0	41.0	34.0	41.0
21	37.935359939376696	40.0	35.0	41.0	34.0	41.0
22	37.89107976827539	40.0	35.0	41.0	34.0	41.0
23	37.88053818524316	40.0	35.0	41.0	34.0	41.0
24	37.81444696956333	40.0	35.0	41.0	34.0	41.0
25	37.742618629241385	40.0	35.0	41.0	34.0	41.0
26	37.6729570505657	40.0	35.0	41.0	34.0	41.0
27	37.72184532926723	40.0	35.0	41.0	34.0	41.0
28	37.70161659593692	40.0	35.0	41.0	34.0	41.0
29	37.65248364574329	40.0	35.0	41.0	34.0	41.0
30	37.60260747713012	40.0	35.0	41.0	33.0	41.0
31	37.46573410268073	40.0	35.0	41.0	33.0	41.0
32	37.235499762614964	40.0	35.0	41.0	33.0	41.0
33	36.98738139376847	40.0	35.0	41.0	32.0	41.0
34	36.787299938870476	40.0	35.0	41.0	31.0	41.0
35	36.63516489247877	40.0	35.0	41.0	31.0	41.0
36	36.57049415204454	40.0	35.0	41.0	31.0	41.0
37	36.49901095959546	40.0	35.0	41.0	31.0	41.0
38	36.41818576472113	40.0	35.0	41.0	30.0	41.0
39	36.389636666669226	40.0	35.0	41.0	30.0	41.0
40	36.33352099151013	39.0	35.0	41.0	30.0	41.0
41	36.31927482130928	40.0	35.0	41.0	30.0	41.0
42	36.25729853526913	39.0	35.0	41.0	30.0	41.0
43	36.17146256027624	39.0	35.0	41.0	30.0	41.0
44	36.01152487097222	39.0	35.0	41.0	29.0	41.0
45	35.89779097691421	39.0	35.0	41.0	28.0	41.0
46	35.855894627121415	39.0	35.0	41.0	28.0	41.0
47	35.78013927100167	39.0	35.0	41.0	28.0	41.0
48	35.73246514581731	39.0	35.0	41.0	28.0	41.0
49	35.664496325709145	39.0	35.0	41.0	28.0	41.0
50	35.512487833537485	38.0	35.0	40.0	27.0	41.0
51	35.39532393661858	38.0	35.0	40.0	27.0	41.0
52	35.072663598174245	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	11.0
16	36.0
17	89.0
18	225.0
19	454.0
20	854.0
21	1406.0
22	2291.0
23	3819.0
24	7236.0
25	13316.0
26	19023.0
27	20661.0
28	18876.0
29	18507.0
30	19870.0
31	23528.0
32	28835.0
33	37561.0
34	66930.0
35	113072.0
36	110242.0
37	101994.0
38	173658.0
39	520133.0
40	1157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.685154576392346	20.434288063482665	26.287842588026127	25.59271477209886
2	32.49505863295364	20.95454095716408	26.055212921722763	20.495187488159512
3	21.935604610868783	22.068678290735694	35.812313188713816	20.183403909681704
4	22.84464740843802	24.89122089540562	33.26174710785257	19.002384588303784
5	20.735180309083756	31.059626979518924	29.497871204619763	18.707321506777554
6	76.88015468760666	1.5595314886074356	18.316077218016105	3.2442366057697987
7	77.59614477495975	1.4388831321632565	15.158587777623527	5.806384315253465
8	67.01605858003097	3.5266442652913934	22.03048192613989	7.4268152285377464
9	34.141260587411	25.07292207558125	24.98740210264084	15.79841523436691
10	24.007642340900254	20.309728031146143	42.57567750609953	13.106952121854073
11	22.860294112007388	17.712375238631402	39.073500390017095	20.35383025934411
12	18.915023826708154	21.642382317997775	41.70881944854574	17.73377440674833
13	15.096154362400666	24.184434751328627	40.8091339933072	19.91027689296351
14	13.965526630459376	23.966147896630513	42.38247139682878	19.68585407608133
15	14.535404118304418	21.04780758236187	44.48818021934531	19.928608079988404
16	15.689578605127055	24.586110175802986	37.52179225319434	22.202518965875615
17	16.076834518468864	21.924406479882865	40.03393187087788	21.96482713077039
18	17.977448804983016	27.276729593515515	38.0836929902001	16.66212861130137
19	18.10139524110113	23.911384434137734	35.62930811657408	22.357912208187063
20	15.467763572173105	28.776512150355614	35.47399157378993	20.281732703681346
21	16.190963415092476	23.153439705351094	36.653093406985334	24.0025034725711
22	14.234818670812533	25.471682918018175	34.117867231584256	26.17563117958504
23	13.57443574075253	22.578346649649596	42.1765331660261	21.670684443571776
24	17.35802342250165	22.47963435801345	37.845004061239976	22.317338158244933
25	14.362216585659182	25.74082155931673	37.04441439527408	22.852547459750006
26	15.884548803525725	22.674988054048622	36.45681931662255	24.9836438258031
27	20.08308092797224	22.61362843220797	36.879050214413525	20.42424042540626
28	16.281852354943936	21.89111888503431	34.99615351870587	26.830875241315887
29	17.12945883114522	22.449874941420735	33.59684734262983	26.82381888480421
30	22.398869755765695	23.316349501338024	31.286274082692827	22.998506660203454
31	18.334024907404494	23.62713598596092	30.747306504350014	27.291532602284573
32	18.117425442307	23.872651172850823	33.30462214361373	24.70530124122845
33	16.837310331656425	25.12967972578385	32.09008512880535	25.942924813754374
34	15.926580144486568	24.59838210017112	34.38731267099201	25.087725084350303
35	17.539187705986166	27.2942170857401	33.057956463814314	22.10863874445942
36	16.473524473668668	28.07409787933477	35.061578215493455	20.390799431503105
37	15.368591083373154	30.799155384805367	34.71597014547599	19.11628338634549
38	14.656589371439704	29.12764258633874	33.857549035925295	22.358219006296267
39	19.294226289683376	25.440773008515947	35.74435740752529	19.520643294275377
40	15.302552790367152	23.176142765432136	38.08192890107218	23.439375543128527
41	18.921466587001422	24.700775969117704	31.005477113244552	25.372280330636322
42	19.00115739586697	20.04227677944821	36.36769446589901	24.588871358785816
43	20.74047257646751	22.704133874422933	33.93677964762703	22.618613901482526
44	16.17945848599735	25.62646256411122	33.73958516293664	24.454493786954792
45	18.384186398259228	25.301793465046874	32.40869496521293	23.905325171480968
46	21.03055018871919	22.953484037677878	32.53325499754945	23.482710776053487
47	16.60184278284293	21.810124184204653	36.80856334882408	24.779469684128337
48	17.618571716742508	19.66821318480214	33.20982152786993	29.503393570585423
49	18.91172574703422	20.36978376102268	36.541955791926455	24.176534700016646
50	18.18262004051269	19.812638394709573	36.79076905849029	25.213972506287448
51	16.36177326239138	19.067885984618677	36.059055568040534	28.511285184949404
52	15.44260612721844	18.971474678801556	42.15283301209015	23.433086181889863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	1682.5
2	2985.0
3	34394.5
4	65804.0
5	37313.5
6	8823.0
7	8545.0
8	8267.0
9	8452.0
10	8637.0
11	8829.0
12	9021.0
13	8843.0
14	8164.0
15	7663.0
16	7362.5
17	7062.0
18	6518.5
19	5975.0
20	5729.5
21	5484.0
22	5319.0
23	5154.0
24	5275.5
25	5397.0
26	6227.0
27	7057.0
28	8195.0
29	9333.0
30	10596.5
31	11860.0
32	13739.0
33	15618.0
34	19051.5
35	22485.0
36	25393.5
37	28302.0
38	31056.0
39	39686.5
40	45563.0
41	57887.5
42	70212.0
43	93826.5
44	117441.0
45	147479.0
46	177517.0
47	180926.0
48	184335.0
49	161434.5
50	138534.0
51	115221.0
52	91908.0
53	74681.5
54	57455.0
55	49191.5
56	40928.0
57	35298.0
58	29668.0
59	26158.0
60	22648.0
61	18764.5
62	14881.0
63	12809.5
64	9219.0
65	7700.0
66	6275.5
67	4851.0
68	3920.0
69	2989.0
70	2779.5
71	2570.0
72	2572.0
73	2574.0
74	2114.5
75	1655.0
76	1339.5
77	1024.0
78	711.0
79	398.0
80	328.0
81	258.0
82	173.5
83	89.0
84	63.5
85	38.0
86	31.0
87	24.0
88	12.0
89	2.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1303789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.04945456046917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22953440482686	23.149171808313433
2	12.08675296711624	7.747476800064159
3	4.962168686863508	4.77104399553045
4	2.498316538833785	3.2027872955608805
5	1.4742656796787834	2.362470545546218
6	1.0162528550023753	1.9542209819007414
7	0.6774294664754764	1.519787143261006
8	0.4831329283727302	1.2387317467638577
9	0.4048848787763351	1.16787055721069
>10	3.560643204348141	25.169210973050255
>50	0.44282407286413045	9.448287101246875
>100	0.15032518072252504	8.682672773781833
>500	0.009380291225800749	2.080844377086622
>1k	0.003126763741933583	1.746062309601729
>5k	7.215608635231346E-4	1.4734881924876186
>10k+	2.4052028784104484E-4	4.285873398593728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55599	4.264417018397915	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	6903	0.5294568369575138	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	6901	0.5293034379029121	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	5311	0.4073511894946191	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2978	0.22841119230182183	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	2916	0.22365582160917144	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	2237	0.1715768425719192	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2134	0.16367679125993548	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1864	0.14296791888871588	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1708	0.13100279262978903	No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1531	0.11742697629754507	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1529	0.11727357724294345	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	1409	0.10806963396684587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5438763480900667	0.0
2	0.0	0.0	0.0	1.853827574860656	0.0
3	0.0	0.0	0.0	3.1084017429200586	0.0
4	0.0	0.0	0.0	3.926402201583232	0.0
5	0.0	0.0	0.0	7.992857740017748	0.0
6	0.0	0.0	0.0	8.940787197928499	0.0
7	0.0	0.0	0.0	12.211255042035177	0.0
8	0.0	0.0	0.0	16.106210437425073	0.0
9	0.0	0.0	0.0	19.14811369017533	0.0
10	0.0	0.0	0.0	21.065064976004553	0.0
11	0.0	0.0	0.0	22.931701371924444	0.0
12	0.0	0.0	0.0	24.229994270545312	0.0
13	0.0	0.0	0.0	24.80692811490203	0.0
14	0.0	0.0	0.0	25.207529745994176	0.0
15	0.0	0.0	0.0	26.540337431900408	0.0
16	0.0	0.0	0.0	28.199808404580804	0.0
17	0.0	0.0	0.0	29.96389753249951	0.0
18	0.0	0.0	0.0	31.000875141606503	0.0
19	0.0	0.0	0.0	32.046289698716585	0.0
20	1.533990546016265E-4	0.0	0.0	33.23950424493534	0.0
21	1.533990546016265E-4	0.0	0.0	34.45327426447071	0.0
22	6.13596218406506E-4	0.0	0.0	35.65300827051003	0.0
23	6.13596218406506E-4	0.0	0.0	36.5931910761634	0.0
24	8.436948003089457E-4	0.0	0.0	37.37920783194213	0.0
25	8.436948003089457E-4	0.0	0.0	38.117133984103255	0.0
26	8.436948003089457E-4	0.0	0.0	38.88412925711139	0.0
27	9.203943276097589E-4	0.0	0.0	39.620598118253795	0.0
28	9.203943276097589E-4	0.0	0.0	40.408839160324256	0.0
29	9.203943276097589E-4	0.0	0.0	41.27776810511517	0.0
30	9.203943276097589E-4	0.0	0.0	42.06539555096722	0.0
31	9.203943276097589E-4	0.0	0.0	42.74526016096163	0.0
32	9.203943276097589E-4	0.0	0.0	43.423360681828115	0.0
33	9.970938549105721E-4	0.0	0.0	44.09356115138262	0.0
34	9.970938549105721E-4	0.0	0.0	44.872905048286185	0.0
35	9.970938549105721E-4	0.0	0.0	45.53927054147565	0.0
36	9.970938549105721E-4	0.0	0.0	46.20970110961206	0.0
37	9.970938549105721E-4	0.0	0.0	46.86878014770795	0.0
38	9.970938549105721E-4	0.0	0.0	47.52064943023756	0.0
39	9.970938549105721E-4	0.0	0.0	48.18087896124296	0.0
40	9.970938549105721E-4	0.0	0.0	48.799844146560524	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCAT	20	6.3117675E-4	46.000004	19
ACGGATA	20	6.3117675E-4	46.000004	4
GTCGTAG	20	6.3117675E-4	46.000004	12
TGTACGA	20	6.3117675E-4	46.000004	12
CCACGTC	20	6.3117675E-4	46.000004	37
CCTGCGT	20	6.3117675E-4	46.000004	22
CGGAGAT	20	6.3117675E-4	46.000004	5
TCGCGAG	20	6.3117675E-4	46.000004	1
GCGATCG	50	1.6370905E-11	46.0	8
CGATCGG	25	3.4174096E-5	46.0	9
TATCCGG	55	1.8189894E-12	46.0	1
CGTTTTT	13930	0.0	45.47164	1
CAACGCA	115	0.0	44.0	16
GCCACGG	105	0.0	43.809525	1
CCCGCAC	105	0.0	43.809525	32
CTAGCGG	300	0.0	43.699997	1
GTCACGG	90	0.0	43.444447	1
TCACGAC	165	0.0	43.21212	24
GGCGATA	320	0.0	43.125004	7
CGGTCTA	155	0.0	43.03226	30
>>END_MODULE