FastQCFastQC Report
Thu 26 May 2016
SRR1043205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043205_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1278993
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524474.10064793161495No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC67420.5271334557734092No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT66160.5172819554133604No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC51460.4023477845461234No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG28380.22189331763348197No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC27010.21118176565469868No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG21390.1672409465884489No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC20680.16168970432207214No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG18310.14315950126388496No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG17150.13408986601177647No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC15440.12071997266599582No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG13740.10742826583100924No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC13200.10320619424813114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG551.8189894E-1245.0000041
CTAGCGG2700.045.0000041
TGATCGG207.033585E-445.01
TCGCATC207.033585E-445.043
CGTGCCG207.033585E-445.040
CGGTTGC207.033585E-445.041
TAACCGG207.033585E-445.01
GTCGTTG351.2120654E-745.023
ACGTCAG207.033585E-445.010
CGTCGAG207.033585E-445.021
AGTACGG800.045.01
CGGACTA207.033585E-445.028
ACGGATG253.8910544E-544.9999964
ACGTCCG253.8910544E-544.99999639
ACCGTTT253.8910544E-544.99999643
TGGGACG253.8910544E-544.9999965
CGTTTTT134250.044.5139661
ATAGCGG1450.043.4482731
TACGCGG1400.043.3928571
GACGGTC1400.043.39285728