##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043205_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1278993
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20330916588285	33.0	31.0	34.0	30.0	34.0
2	32.49321614739095	34.0	31.0	34.0	31.0	34.0
3	32.580443364428106	34.0	31.0	34.0	31.0	34.0
4	36.13607580338594	37.0	35.0	37.0	35.0	37.0
5	36.088722924988645	37.0	35.0	37.0	35.0	37.0
6	36.330252784808046	37.0	37.0	37.0	35.0	37.0
7	36.26571763879865	37.0	37.0	37.0	35.0	37.0
8	36.16213302183827	37.0	37.0	37.0	35.0	37.0
9	37.68592322241013	39.0	38.0	39.0	35.0	39.0
10	37.46127304840605	39.0	37.0	39.0	35.0	39.0
11	37.2774698532361	39.0	37.0	39.0	34.0	39.0
12	37.30402277416687	39.0	37.0	39.0	34.0	39.0
13	37.318703073433554	39.0	37.0	39.0	34.0	39.0
14	38.69472155046979	40.0	38.0	41.0	35.0	41.0
15	38.78503244349265	40.0	38.0	41.0	35.0	41.0
16	38.834937329602276	40.0	38.0	41.0	35.0	41.0
17	38.78120755938461	40.0	38.0	41.0	35.0	41.0
18	38.579716229877725	40.0	38.0	41.0	35.0	41.0
19	38.368679891133105	40.0	37.0	41.0	35.0	41.0
20	38.09849780256811	40.0	35.0	41.0	34.0	41.0
21	38.045833714492574	40.0	35.0	41.0	34.0	41.0
22	38.008481672690934	40.0	35.0	41.0	34.0	41.0
23	37.96192160551309	40.0	35.0	41.0	34.0	41.0
24	37.90050610128437	40.0	35.0	41.0	34.0	41.0
25	37.89370778417083	40.0	35.0	41.0	34.0	41.0
26	37.848485488192665	40.0	35.0	41.0	34.0	41.0
27	37.86638238051342	40.0	35.0	41.0	34.0	41.0
28	37.81467607719511	40.0	35.0	41.0	34.0	41.0
29	37.785955044320026	40.0	35.0	41.0	34.0	41.0
30	37.688920111368866	40.0	35.0	41.0	34.0	41.0
31	37.53345014398046	40.0	35.0	41.0	33.0	41.0
32	37.315415330654666	40.0	35.0	41.0	33.0	41.0
33	37.02921204416287	40.0	35.0	41.0	32.0	41.0
34	36.77807462589709	40.0	35.0	41.0	31.0	41.0
35	36.62788459358261	40.0	35.0	41.0	31.0	41.0
36	36.548246159283124	40.0	35.0	41.0	31.0	41.0
37	36.4300938316316	40.0	35.0	41.0	30.0	41.0
38	36.339136336164465	39.0	35.0	41.0	30.0	41.0
39	36.25254712105539	39.0	35.0	41.0	30.0	41.0
40	36.174424723200204	39.0	35.0	41.0	30.0	41.0
41	36.13070126263396	39.0	35.0	41.0	30.0	41.0
42	36.05583846041378	39.0	35.0	41.0	30.0	41.0
43	35.945823784805704	39.0	35.0	41.0	29.0	41.0
44	35.84082633759528	39.0	35.0	41.0	28.0	41.0
45	35.75750453677229	39.0	35.0	41.0	28.0	41.0
46	35.65181513894134	39.0	35.0	40.0	27.0	41.0
47	35.5240802725269	38.0	35.0	40.0	27.0	41.0
48	35.5061489781414	38.0	35.0	40.0	27.0	41.0
49	35.42221419507378	38.0	35.0	40.0	27.0	41.0
50	35.30168421562902	38.0	35.0	40.0	26.0	41.0
51	34.88846146929655	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	4.0
15	13.0
16	30.0
17	70.0
18	182.0
19	372.0
20	730.0
21	1166.0
22	2019.0
23	3389.0
24	5943.0
25	11355.0
26	18045.0
27	20888.0
28	19894.0
29	19125.0
30	20301.0
31	23252.0
32	28252.0
33	36616.0
34	66625.0
35	110760.0
36	104953.0
37	102178.0
38	183526.0
39	499184.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.882638919837717	20.577438656818295	26.360973046764137	25.178949376579858
2	32.384149092293704	20.93045075305338	26.12641351438202	20.558986640270902
3	22.02482734463754	22.207471033852414	35.411061671174124	20.35663995033593
4	22.991290804562652	24.990128953012253	32.900336436555946	19.11824380586915
5	20.964461885248788	31.114009224444544	29.13260666790201	18.788922222404658
6	77.27016488753262	1.5361303775704793	17.940442207267747	3.2532625276291585
7	77.98408591759298	1.3884360586805402	14.84417819331302	5.783299830413458
8	67.2303132229809	3.4998627826735564	21.708171975921683	7.56165201842387
9	34.28619234038028	25.093178774238794	24.714130569909294	15.906498315471627
10	24.187388046689858	20.337171509148213	42.291083688495554	13.184356755666371
11	23.010681059239573	17.700722365173228	38.95752361428092	20.33107296130628
12	19.2303632623478	21.48784238850408	41.47419102371944	17.80760332542868
13	15.190778995662994	24.165261264135143	40.656125561281414	19.987834178920448
14	14.05801282727896	23.8497786930812	42.1987454192478	19.89346306039204
15	14.595075969923213	20.997769338847046	44.36419902219949	20.042955669030242
16	15.834410352519521	24.418898305151004	37.43609230073972	22.31059904158975
17	16.26396704282197	21.861417537077998	39.89099236665095	21.98362305344908
18	18.09595517723709	27.1194603879771	38.050794648602455	16.733789786183348
19	18.18977899018994	23.924524997400297	35.48221139599669	22.40348461641307
20	15.555597255027978	28.747694475262957	35.26626025318356	20.430448016525503
21	16.185311412963166	23.14054885366847	36.35586746760928	24.318272265759077
22	14.263956096710459	25.388254665975495	34.03388447004792	26.313904767266123
23	13.64276426845182	22.56517432073514	41.969502569599676	21.82255884121336
24	17.414168803113075	22.459231598609218	37.66932266243834	22.457276935839367
25	14.295856193114426	25.675199160589617	37.04594161187747	22.983003034418484
26	15.808843363489872	22.56845815418849	36.401450203402206	25.221248278919433
27	20.135841243853562	22.456964189796192	36.868067299821035	20.539127266529214
28	16.360058264587842	21.946484460821914	34.782285751368455	26.911171523221782
29	17.24395676911445	22.373617369289747	33.314803130275145	27.067622731320657
30	22.49433734195574	23.341175440365973	31.01729251059232	23.147194707085966
31	18.37719205656325	23.594421548827867	30.394146019563827	27.634240375045056
32	18.190482668787084	23.79520450854696	32.99032911047989	25.02398371218607
33	17.00517516514946	24.970582325313742	31.800564975726996	26.223677533809802
34	16.142074272494064	24.433753742201873	34.07188311429382	25.35228887101024
35	17.526679192145696	27.134941317114325	32.96828051443597	22.370098976304014
36	16.492115281318974	28.06152965653448	34.964851254072535	20.48150380807401
37	15.491406129666075	30.791646240440723	34.55398113984987	19.16296649004334
38	14.712433922625065	29.18092593157273	33.628721971113215	22.477918174688995
39	19.37391369616566	25.337198874426992	35.64624669564259	19.642640733764765
40	15.362163827323528	23.10043917363113	38.011310460651465	23.52608653839388
41	18.925435870251047	24.537194495982387	30.995009355016016	25.542360278750547
42	19.207767360728322	19.860859285390926	36.18190248109255	24.7494708727882
43	20.845383829309462	22.482609365336636	33.861326840725475	22.81067996462842
44	16.28937765883003	25.450569315078347	33.75038018190873	24.509672844182884
45	18.42332209793173	25.146814720643505	32.433172034561565	23.996691146863196
46	21.140850653600136	22.873620105817626	32.397518985639486	23.588010254942755
47	16.76694086676002	21.65234680721474	36.731397278953054	24.84931504707219
48	17.762724268232898	19.468363001204857	33.108312555268085	29.660600175294157
49	19.02363812780836	20.187366154466837	36.47361635286511	24.315379364859698
50	18.269294671667478	19.637167678009185	36.76423561348655	25.329302036836793
51	16.45935513329627	19.04959604939198	35.85633385014617	28.634714967165575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	1565.0
2	2810.0
3	32564.0
4	62318.0
5	35771.5
6	9225.0
7	8742.0
8	8259.0
9	8412.5
10	8566.0
11	8672.0
12	8778.0
13	8395.5
14	8013.0
15	7533.0
16	7053.0
17	6728.5
18	6404.0
19	5888.0
20	5372.0
21	5311.0
22	5250.0
23	4963.0
24	4676.0
25	5138.5
26	6449.5
27	7298.0
28	8159.0
29	9020.0
30	10920.0
31	12820.0
32	14661.0
33	16502.0
34	20753.0
35	25004.0
36	27365.5
37	29727.0
38	33946.0
39	38165.0
40	45654.0
41	53143.0
42	67068.0
43	80993.0
44	107392.0
45	133791.0
46	161256.0
47	188721.0
48	185269.5
49	181818.0
50	149454.5
51	117091.0
52	95287.5
53	73484.0
54	61503.5
55	49523.0
56	42345.0
57	35167.0
58	30861.0
59	26555.0
60	22168.5
61	17782.0
62	14986.5
63	12191.0
64	10534.0
65	8877.0
66	7361.5
67	5846.0
68	4680.0
69	3514.0
70	3185.0
71	2856.0
72	2685.5
73	2515.0
74	2166.0
75	1499.5
76	1182.0
77	810.5
78	439.0
79	370.5
80	302.0
81	207.0
82	112.0
83	75.5
84	39.0
85	42.0
86	45.0
87	24.0
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1278993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.756306040257993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63843789203816	22.74972157945578
2	12.3996609975253	7.875348588657283
3	5.006622907620718	4.769755478477096
4	2.557846386182379	3.2491101057430223
5	1.4810186093807731	2.3515840105406567
6	0.993585822877249	1.893156928113089
7	0.7173144011736258	1.594547895552784
8	0.5151020842099002	1.3086191542515468
9	0.3970085704596259	1.1346773097709089
>10	3.6835706534117634	25.6330125690969
>50	0.44284159449439675	9.344571896819696
>100	0.15406822918402918	8.845494631035047
>500	0.00894589715221684	1.9898042370923363
>1k	0.002981965717405613	1.6616029354041735
>5k	7.454914293514032E-4	1.4602156495402177
>10k+	2.4849714311713444E-4	4.138777030449406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52447	4.10064793161495	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	6742	0.5271334557734092	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	6616	0.5172819554133604	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	5146	0.4023477845461234	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	2838	0.22189331763348197	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2701	0.21118176565469868	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	2139	0.1672409465884489	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	2068	0.16168970432207214	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1831	0.14315950126388496	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1715	0.13408986601177647	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1544	0.12071997266599582	No Hit
TCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1374	0.10742826583100924	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1320	0.10320619424813114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5319810194426396	0.0
2	0.0	0.0	0.0	1.8359756464656178	0.0
3	0.0	0.0	0.0	3.097358625105845	0.0
4	0.0	0.0	0.0	3.9240246037312168	0.0
5	0.0	0.0	0.0	7.995821712863167	0.0
6	0.0	0.0	0.0	8.961581494191133	0.0
7	0.0	0.0	0.0	12.25448458279287	0.0
8	0.0	0.0	0.0	16.246453264404106	0.0
9	0.0	0.0	0.0	19.388612760194935	0.0
10	0.0	0.0	0.0	21.381899666378157	0.0
11	0.0	0.0	0.0	23.287774053493646	0.0
12	0.0	0.0	0.0	24.60771872871861	0.0
13	0.0	0.0	0.0	25.197088647084072	0.0
14	0.0	0.0	0.0	25.627583575516052	0.0
15	0.0	0.0	0.0	27.010077459376244	0.0
16	0.0	0.0	0.0	28.686239877778846	0.0
17	0.0	0.0	0.0	30.519322623345086	0.0
18	0.0	0.0	0.0	31.584692019424658	0.0
19	0.0	0.0	0.0	32.6635876818716	0.0
20	3.12746043176155E-4	0.0	0.0	33.900732842165674	0.0
21	3.12746043176155E-4	0.0	0.0	35.1700126583961	0.0
22	4.691190647642325E-4	0.0	0.0	36.411692636316225	0.0
23	4.691190647642325E-4	0.0	0.0	37.37010288562956	0.0
24	6.2549208635231E-4	0.0	0.0	38.16494695436175	0.0
25	6.2549208635231E-4	0.0	0.0	38.92789092668998	0.0
26	6.2549208635231E-4	0.0	0.0	39.70451753840717	0.0
27	7.036785971463488E-4	0.0	0.0	40.45612446667026	0.0
28	7.036785971463488E-4	0.0	0.0	41.23814594763224	0.0
29	7.036785971463488E-4	0.0	0.0	42.12079346798614	0.0
30	7.036785971463488E-4	0.0	0.0	42.90805344517132	0.0
31	7.036785971463488E-4	0.0	0.0	43.609777379547815	0.0
32	7.036785971463488E-4	0.0	0.0	44.29187649971501	0.0
33	7.036785971463488E-4	0.0	0.0	44.968737123659004	0.0
34	7.036785971463488E-4	0.0	0.0	45.7438781916711	0.0
35	7.036785971463488E-4	0.0	0.0	46.41933145842081	0.0
36	7.036785971463488E-4	0.0	0.0	47.090484467076834	0.0
37	7.036785971463488E-4	0.0	0.0	47.75405338418584	0.0
38	7.036785971463488E-4	0.0	0.0	48.41707499571929	0.0
39	7.036785971463488E-4	0.0	0.0	49.07188702361936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	55	1.8189894E-12	45.000004	1
CTAGCGG	270	0.0	45.000004	1
TGATCGG	20	7.033585E-4	45.0	1
TCGCATC	20	7.033585E-4	45.0	43
CGTGCCG	20	7.033585E-4	45.0	40
CGGTTGC	20	7.033585E-4	45.0	41
TAACCGG	20	7.033585E-4	45.0	1
GTCGTTG	35	1.2120654E-7	45.0	23
ACGTCAG	20	7.033585E-4	45.0	10
CGTCGAG	20	7.033585E-4	45.0	21
AGTACGG	80	0.0	45.0	1
CGGACTA	20	7.033585E-4	45.0	28
ACGGATG	25	3.8910544E-5	44.999996	4
ACGTCCG	25	3.8910544E-5	44.999996	39
ACCGTTT	25	3.8910544E-5	44.999996	43
TGGGACG	25	3.8910544E-5	44.999996	5
CGTTTTT	13425	0.0	44.513966	1
ATAGCGG	145	0.0	43.448273	1
TACGCGG	140	0.0	43.392857	1
GACGGTC	140	0.0	43.392857	28
>>END_MODULE