##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043204_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2408193
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.362759131016496	33.0	31.0	34.0	31.0	34.0
2	32.69237100182585	34.0	31.0	34.0	31.0	34.0
3	32.795442890167024	34.0	31.0	34.0	31.0	34.0
4	36.226841868571164	37.0	37.0	37.0	35.0	37.0
5	36.173211200265094	37.0	37.0	37.0	35.0	37.0
6	36.33415178932918	37.0	37.0	37.0	35.0	37.0
7	36.27861886485012	37.0	37.0	37.0	35.0	37.0
8	36.26949085891372	37.0	37.0	37.0	35.0	37.0
9	37.8072309819022	39.0	38.0	39.0	35.0	39.0
10	37.703025048241564	39.0	37.0	39.0	35.0	39.0
11	37.45009432383534	39.0	37.0	39.0	35.0	39.0
12	37.452325042054355	39.0	37.0	39.0	35.0	39.0
13	37.43851800914628	39.0	37.0	39.0	35.0	39.0
14	38.83786100200441	41.0	38.0	41.0	35.0	41.0
15	38.88345950677541	41.0	38.0	41.0	35.0	41.0
16	38.78960614867662	41.0	38.0	41.0	35.0	41.0
17	38.7649698342284	41.0	38.0	41.0	34.0	41.0
18	38.52463403057811	40.0	38.0	41.0	34.0	41.0
19	38.249121644320034	40.0	37.0	41.0	34.0	41.0
20	37.95548321916059	40.0	35.0	41.0	34.0	41.0
21	37.87188651407923	40.0	35.0	41.0	34.0	41.0
22	37.820572520557945	40.0	35.0	41.0	34.0	41.0
23	37.80806521736422	40.0	35.0	41.0	34.0	41.0
24	37.71309691540504	40.0	35.0	41.0	34.0	41.0
25	37.621031619973984	39.0	35.0	41.0	34.0	41.0
26	37.53235932502088	39.0	35.0	41.0	34.0	41.0
27	37.54100688773699	39.0	35.0	41.0	34.0	41.0
28	37.51045493446746	39.0	35.0	41.0	34.0	41.0
29	37.45865177749458	39.0	35.0	41.0	33.0	41.0
30	37.324348173090776	39.0	35.0	41.0	33.0	41.0
31	37.21617287318749	39.0	35.0	41.0	33.0	41.0
32	36.929497345104814	39.0	35.0	41.0	33.0	41.0
33	36.50163919586179	39.0	35.0	41.0	31.0	41.0
34	36.20080367312753	39.0	35.0	41.0	30.0	41.0
35	36.00256748524724	39.0	35.0	41.0	29.0	41.0
36	35.93727246944078	39.0	35.0	41.0	29.0	41.0
37	35.8763579164959	39.0	35.0	41.0	29.0	41.0
38	35.78735093076012	39.0	35.0	41.0	27.0	41.0
39	35.78862948276986	39.0	35.0	41.0	28.0	41.0
40	35.70584874218968	39.0	35.0	41.0	27.0	41.0
41	35.669242041646996	39.0	35.0	41.0	27.0	41.0
42	35.583977696139804	39.0	35.0	41.0	27.0	41.0
43	35.47597763136094	39.0	35.0	41.0	26.0	41.0
44	35.305663624136436	38.0	35.0	41.0	25.0	41.0
45	35.1938299795739	38.0	35.0	41.0	24.0	41.0
46	35.158335316147834	38.0	35.0	41.0	24.0	41.0
47	35.05239862419665	38.0	35.0	40.0	24.0	41.0
48	34.947703527084414	37.0	35.0	40.0	23.0	41.0
49	34.88707258928167	37.0	35.0	40.0	24.0	41.0
50	34.762943003322405	37.0	35.0	40.0	24.0	41.0
51	34.621783220863115	37.0	35.0	40.0	24.0	41.0
52	34.16897690509025	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	9.0
14	19.0
15	42.0
16	97.0
17	197.0
18	510.0
19	1040.0
20	1706.0
21	2922.0
22	4652.0
23	8074.0
24	14752.0
25	27465.0
26	40920.0
27	46316.0
28	44741.0
29	41417.0
30	41624.0
31	46328.0
32	53698.0
33	68227.0
34	141872.0
35	201139.0
36	210689.0
37	195347.0
38	333414.0
39	878996.0
40	1976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.49148427887632	17.270376585265385	23.89443038826207	31.343708747596228
2	36.78405343757747	19.090870208492426	24.179208227911968	19.94586812601814
3	24.269234234963726	20.156731624084948	36.77209426320897	18.801939877742356
4	21.651794519791395	23.05932290310619	35.05537139257526	20.233511184527153
5	19.982160898233655	30.60925764670855	31.46824195569043	17.94033949936737
6	75.65519042701312	1.818957201519978	19.595896176095522	2.929956195371384
7	75.87905122222347	1.5675238654044754	17.70090686253137	4.85251804984069
8	67.18498060579032	2.906992919587425	23.133154194867274	6.774872279754987
9	33.38727419272459	16.84391574927757	28.29212608790076	21.47668397009708
10	27.000244581725802	19.7342571795533	36.93943965454596	16.32605858417494
11	25.500198696699144	15.598832817801563	38.36175090617737	20.53921757932192
12	21.494747306382838	16.158173368994927	41.16974843793666	21.177330886685578
13	17.58322526475245	19.742977410863663	40.471839258730505	22.201958065653375
14	16.624290494989395	20.49910451529425	41.607047275695926	21.26955771402043
15	19.00092725126267	16.71485632588418	43.12399379950029	21.160222623352862
16	18.28732165569786	21.115209619826985	39.13349137714461	21.46397734733055
17	18.135506581075518	21.663089295583866	39.777252072404494	20.42415205093612
18	19.832712743538412	26.843944816715272	36.19979793978307	17.123544499963252
19	19.570773604939472	22.368348384037326	35.71727847394291	22.34359953708029
20	19.44275230432112	26.434260044772156	35.96862876023641	18.15435889067031
21	16.951797468060075	21.63393880806065	36.86070842328667	24.553555300592603
22	17.33420037347505	24.028805000263684	35.458412178758095	23.178582447503167
23	18.237823961783796	21.84123116378131	39.495464026346724	20.425480848088174
24	22.000977496404982	20.514676356919896	35.76843716429705	21.715908982378075
25	17.470194457005732	21.52838248429424	37.826910052475036	23.174513006225002
26	17.374230387680722	19.688413677807386	38.4151104168146	24.522245517697293
27	21.223714212274515	20.361407910412495	38.352740000489995	20.062137876822998
28	20.319716899766753	22.047609971459927	34.6828098910677	22.949863237705614
29	23.345055815709124	19.33781885421974	36.36054087027078	20.956584459800357
30	23.263999189433736	19.275905211916154	36.303776316931405	21.156319281718698
31	24.372838887913055	19.088877012764343	33.60478167655167	22.933502422770932
32	25.226549533197712	18.286283532922816	34.17014333984029	22.31702359403918
33	20.756226764216986	19.236331971731502	37.741368735811456	22.266072528240056
34	21.084937959706718	19.039877617782295	37.42004897448004	22.45513544803095
35	23.884796608909667	21.040713929489872	34.758053029802845	20.31643643179762
36	21.915851428851425	22.689875769923756	34.78176375398483	20.612509047239985
37	22.875367547368505	22.56521798709655	36.35219436315943	18.207220102375516
38	20.654947506283758	22.485656257617226	33.40018844004612	23.45920779605289
39	24.20923073856622	20.770262184135575	35.774541326214305	19.245965751083904
40	19.21278734719352	20.77184013075364	38.12103099710031	21.894341524952527
41	20.642739182449247	24.17904212826796	32.55910136770599	22.6191173215768
42	19.833958490868465	19.278687380953272	35.07509572530109	25.81225840287718
43	22.83164181608368	20.566997744782086	34.705440967563646	21.895919471570593
44	21.53050025475533	20.62841308815365	35.665704534478756	22.175382122612266
45	22.547902099208823	20.323495666667913	34.57114940538404	22.557452828739226
46	23.168491894129748	20.310166170236354	33.619190820669274	22.90215111496462
47	20.006785170457682	21.187629064614008	35.652042838759186	23.153542926169123
48	20.689703856792207	19.460732590784875	33.20821877648511	26.64134477593781
49	20.705483322972867	16.917373316839637	37.62401933732056	24.75312402286694
50	19.74530280587976	17.546434193604917	38.65624557500167	24.052017425513654
51	18.448438310384592	16.53480431178066	37.9531873068313	27.063570071003447
52	16.664029834818056	17.91600590152035	40.503190566536816	24.916773697124775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	512.0
1	2774.0
2	5036.0
3	80209.5
4	155383.0
5	89011.5
6	22640.0
7	21157.0
8	19674.0
9	19480.0
10	19286.0
11	19079.0
12	18872.0
13	18126.0
14	16409.5
15	15439.0
16	14542.0
17	13645.0
18	12845.0
19	12045.0
20	11356.5
21	10668.0
22	10244.5
23	9821.0
24	9566.5
25	9312.0
26	10237.0
27	11162.0
28	12064.5
29	12967.0
30	14031.0
31	15095.0
32	17521.5
33	19948.0
34	23304.0
35	26660.0
36	30990.0
37	35320.0
38	38940.5
39	45649.0
40	48737.0
41	58435.5
42	68134.0
43	82854.5
44	97575.0
45	127567.0
46	157559.0
47	198871.5
48	240184.0
49	261039.0
50	281894.0
51	265472.5
52	249051.0
53	211174.0
54	173297.0
55	152539.5
56	131782.0
57	118330.5
58	104879.0
59	95204.0
60	85529.0
61	80377.5
62	75226.0
63	73443.5
64	57372.0
65	43083.0
66	34889.5
67	26696.0
68	20839.5
69	14983.0
70	13567.0
71	12151.0
72	14498.5
73	16846.0
74	12509.0
75	8172.0
76	5943.5
77	3715.0
78	2855.0
79	1995.0
80	1477.0
81	959.0
82	670.0
83	381.0
84	274.0
85	167.0
86	119.5
87	72.0
88	43.5
89	14.5
90	14.0
91	9.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2408193.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.300737708471033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6758094507818	23.687086624951544
2	10.343184053885352	6.47498582282211
3	3.676082980707461	3.4519232752109588
4	1.8698729177979423	2.3411360699266726
5	1.088992075632055	1.7043127662981206
6	0.7950614021119835	1.4931605045781848
7	0.5606628544717187	1.2284412665491355
8	0.4856772253344173	1.2161644352936465
9	0.40974811091049596	1.154287633153584
>10	4.620934645220227	31.012222505190696
>50	0.3225012006319469	6.630787589632173
>100	0.1405208922487669	7.847512300559657
>500	0.005876785020664317	1.2735452234401068
>1k	0.004006898877725671	2.639231525318384
>5k	6.678164796209452E-4	1.630570999239552
>10k+	4.006898877725671E-4	6.214631457835478
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119874	4.9777571814219215	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	17639	0.7324579051595947	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	11140	0.46258750855932224	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	9911	0.4115533929381906	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT	8368	0.34748045526251425	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	7367	0.305914019349778	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	7175	0.297941236437445	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	6182	0.25670699981272266	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	4879	0.2026000407774626	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT	4391	0.18233588420861616	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4237	0.17594104791434906	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3540	0.14699818494614011	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	3280	0.13620170808568913	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	3272	0.13586950879767526	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2757	0.11448417963178201	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT	2711	0.11257403372570221	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2661	0.1104977881756155	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35989640365203285	0.0
2	0.0	0.0	0.0	1.1394020329765928	0.0
3	0.0	0.0	0.0	1.9724332725823885	0.0
4	0.0	0.0	0.0	2.498014071131342	0.0
5	0.0	0.0	0.0	5.211750054916695	0.0
6	0.0	0.0	0.0	6.2485440328080015	0.0
7	0.0	0.0	0.0	8.955885180299088	0.0
8	0.0	0.0	0.0	11.674230429205632	0.0
9	4.1524911001734496E-5	0.0	0.0	14.549290692232724	0.0
10	4.1524911001734496E-5	0.0	0.0	16.122794144821448	0.0
11	4.1524911001734496E-5	0.0	0.0	17.327888587002786	0.0
12	4.1524911001734496E-5	0.0	0.0	18.21689540663892	0.0
13	8.304982200346899E-5	0.0	0.0	18.66000773193843	0.0
14	8.304982200346899E-5	0.0	0.0	18.969991192566376	0.0
15	8.304982200346899E-5	0.0	0.0	19.787948889478542	0.0
16	8.304982200346899E-5	0.0	0.0	20.66537025894519	0.0
17	8.304982200346899E-5	0.0	0.0	21.730982525071703	0.0
18	8.304982200346899E-5	0.0	0.0	22.297548410779367	0.0
19	8.304982200346899E-5	0.0	0.0	22.963026634493165	0.0
20	8.304982200346899E-5	0.0	0.0	23.72828921934413	0.0
21	8.304982200346899E-5	0.0	0.0	24.462491170765798	0.0
22	8.304982200346899E-5	0.0	0.0	25.251298380154747	0.0
23	8.304982200346899E-5	0.0	0.0	25.892110806733513	0.0
24	8.304982200346899E-5	0.0	0.0	26.461708010944307	0.0
25	8.304982200346899E-5	0.0	0.0	26.984506640456143	0.0
26	8.304982200346899E-5	0.0	0.0	27.540732823324376	0.0
27	8.304982200346899E-5	0.0	0.0	28.104059765973908	0.0
28	8.304982200346899E-5	0.0	0.0	28.665891811827375	0.0
29	8.304982200346899E-5	0.0	0.0	29.237648311410258	0.0
30	8.304982200346899E-5	0.0	0.0	29.748446241642593	0.0
31	8.304982200346899E-5	0.0	0.0	30.25405355799971	0.0
32	1.2457473300520348E-4	0.0	0.0	30.79910953980848	0.0
33	1.2457473300520348E-4	0.0	0.0	31.336192738704913	0.0
34	1.6609964400693798E-4	0.0	0.0	31.909444135083856	0.0
35	1.6609964400693798E-4	0.0	0.0	32.40109908134439	0.0
36	1.6609964400693798E-4	0.0	0.0	32.91443003114784	0.0
37	1.6609964400693798E-4	0.0	0.0	33.47775697379737	0.0
38	2.076245550086725E-4	0.0	0.0	34.07995123314452	0.0
39	2.076245550086725E-4	0.0	0.0	34.70710196400371	0.0
40	2.076245550086725E-4	0.0	0.0	35.31220296712099	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32155	0.0	45.563675	1
TCGGCGT	1570	0.0	43.21656	4
TACGGGT	240	0.0	43.125	3
GCGTCCC	1625	0.0	42.886154	7
ACTACGG	195	0.0	42.461536	1
CGACGGT	450	0.0	42.422226	27
GTCGGCG	1640	0.0	42.07317	3
AGGGCGC	1140	0.0	41.964912	5
CACGACG	455	0.0	41.956043	25
CTAGCGG	440	0.0	41.81818	1
GCACTTA	4415	0.0	41.780296	35
ACCCGCA	4485	0.0	41.589745	31
CGCACTT	4430	0.0	41.586906	34
CGGCGTC	1655	0.0	41.55287	5
CCCGCAC	4505	0.0	41.45616	32
CCGCACT	4450	0.0	41.348316	33
TCGACGT	95	0.0	41.157894	25
GTTTTTT	35900	0.0	41.12451	2
CGTTTCT	700	0.0	40.74286	1
TCACGAC	480	0.0	40.729168	24
>>END_MODULE