FastQCFastQC Report
Thu 26 May 2016
SRR1043203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043203_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2345915
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1124634.793992962234352No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC170360.7261985195542038No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC94440.4025721307037979No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG92270.393322008683179No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT81460.34724190774175534Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG73170.3119038839855664No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC66740.28449453624705073No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT59710.2545275510834792No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC46840.19966622831603023No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT41530.17703113710428553No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC40770.17379146303254808No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC34990.14915288917117628No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT31130.13269875506998335No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCC29590.1261341523456732No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC26380.11245079212162418No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC26240.11185401005577782No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC25320.10793229933735878No Hit
GGGCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC23720.10111193287054304No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA23580.10051515080469668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT207.034791E-445.012
ACAATCG207.034791E-445.031
CGTTTTT316350.044.431011
ACTACGG1200.043.1249961
CTAGCGG4850.042.680411
ATTACGG1650.042.2727281
CACGACG4100.041.70731725
TCGGCGT14450.041.5743984
CGCACTT43500.041.17241334
GCGTCCC14600.041.1472637
TACGGGT2250.041.03
TAGGGCG6650.040.939854
GTCGGCG14750.040.7288133
CCGCACT44350.040.43404833
ACCCGCA44600.040.35874631
CGTTTTC5300.040.330191
GCACTTA44350.040.2818535
CCCGCAC45250.040.27624532
GTTTTTT352100.040.2328872
TAGGGCA11750.040.212774
Produced by FastQC (version 0.11.5)