##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043203_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2345915
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32506164971877	33.0	31.0	34.0	31.0	34.0
2	32.62331073376486	34.0	31.0	34.0	31.0	34.0
3	32.71043537383068	34.0	31.0	34.0	31.0	34.0
4	36.19548918012801	37.0	37.0	37.0	35.0	37.0
5	36.187060059720835	37.0	37.0	37.0	35.0	37.0
6	36.35225615591358	37.0	37.0	37.0	35.0	37.0
7	36.30765479567674	37.0	37.0	37.0	35.0	37.0
8	36.22436916938593	37.0	37.0	37.0	35.0	37.0
9	37.76810881894698	39.0	38.0	39.0	35.0	39.0
10	37.64920851778517	39.0	37.0	39.0	35.0	39.0
11	37.43409160178438	39.0	37.0	39.0	35.0	39.0
12	37.443437208935535	39.0	37.0	39.0	35.0	39.0
13	37.44324197594542	39.0	37.0	39.0	35.0	39.0
14	38.83765311189877	41.0	38.0	41.0	35.0	41.0
15	38.893315401453165	41.0	38.0	41.0	35.0	41.0
16	38.837993277676304	41.0	38.0	41.0	35.0	41.0
17	38.78566955750741	41.0	38.0	41.0	34.0	41.0
18	38.570003175733135	40.0	38.0	41.0	34.0	41.0
19	38.318419465325896	40.0	37.0	41.0	35.0	41.0
20	38.045853323756404	40.0	35.0	41.0	34.0	41.0
21	37.96213162028462	40.0	35.0	41.0	34.0	41.0
22	37.927881018706984	40.0	35.0	41.0	34.0	41.0
23	37.880806423080124	40.0	35.0	41.0	34.0	41.0
24	37.795563777886244	40.0	35.0	41.0	34.0	41.0
25	37.76472719599815	40.0	35.0	41.0	34.0	41.0
26	37.72047793718016	40.0	35.0	41.0	34.0	41.0
27	37.68906759196305	39.0	35.0	41.0	34.0	41.0
28	37.606680548954245	39.0	35.0	41.0	34.0	41.0
29	37.5912311400882	39.0	35.0	41.0	34.0	41.0
30	37.41964222915153	39.0	35.0	41.0	34.0	41.0
31	37.29439770835687	39.0	35.0	41.0	33.0	41.0
32	37.021256098366734	39.0	35.0	41.0	33.0	41.0
33	36.582726143104075	39.0	35.0	41.0	31.0	41.0
34	36.24507026043143	39.0	35.0	41.0	30.0	41.0
35	36.05259184582562	39.0	35.0	41.0	30.0	41.0
36	35.9617108889282	39.0	35.0	41.0	29.0	41.0
37	35.862578141151744	39.0	35.0	41.0	29.0	41.0
38	35.76427193653649	39.0	35.0	41.0	27.0	41.0
39	35.71322831389884	39.0	35.0	41.0	27.0	41.0
40	35.60443195938472	39.0	35.0	41.0	27.0	41.0
41	35.52701056943666	39.0	35.0	41.0	26.0	41.0
42	35.42555207669502	38.0	35.0	41.0	26.0	41.0
43	35.30278931674847	38.0	35.0	41.0	25.0	41.0
44	35.17367551680261	38.0	35.0	40.0	24.0	41.0
45	35.09238740534077	38.0	35.0	40.0	24.0	41.0
46	35.00697510353103	38.0	35.0	40.0	23.0	41.0
47	34.84764239113523	38.0	35.0	40.0	23.0	41.0
48	34.7720616475874	37.0	35.0	40.0	23.0	41.0
49	34.69376725073159	37.0	35.0	40.0	24.0	41.0
50	34.60353593374014	37.0	35.0	40.0	24.0	41.0
51	34.13146171110206	36.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	7.0
14	19.0
15	41.0
16	90.0
17	189.0
18	418.0
19	830.0
20	1606.0
21	2541.0
22	4082.0
23	6692.0
24	11787.0
25	23052.0
26	37815.0
27	46000.0
28	45147.0
29	42025.0
30	41803.0
31	45811.0
32	51813.0
33	65555.0
34	137512.0
35	199382.0
36	198254.0
37	191117.0
38	344903.0
39	847219.0
40	201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.75569447315866	17.377952739123113	23.90448929309033	30.961863494627895
2	36.478261147569285	19.219451685163357	24.20846450105822	20.09382266620913
3	24.426716227996327	20.256403151861853	36.36308220886094	18.953798411280886
4	21.80590515854155	23.23374887836942	34.58518318012375	20.37516278296528
5	20.1189301402651	30.73922115677678	31.12525389879855	18.01659480415957
6	76.0597464102493	1.798146991685547	19.1984790582779	2.943627539787247
7	76.28724825920803	1.554617281529808	17.320960051834785	4.837174407427379
8	67.59818663506564	2.881775341391312	22.7783189075478	6.741719115995251
9	33.59375765959125	16.8946871476588	27.892314938947067	21.61924025380289
10	27.330316742081447	19.75276171557793	36.53444391633968	16.38247762600094
11	25.829367219187393	15.632066805489542	37.99587794101662	20.542688034306444
12	21.74166583188223	16.17398754856847	40.9161030983646	21.1682435211847
13	17.70106760048851	19.78750295726827	40.213051197507156	22.29837824473606
14	16.77209106041779	20.514340886178744	41.38611160250904	21.327456450894427
15	19.20214500525381	16.67217269167894	42.87486972034366	21.250812582723587
16	18.4611548159247	21.030003218360427	38.93065179258413	21.57819017313074
17	18.365413921646777	21.597756099432416	39.49158430718931	20.5452456717315
18	20.044758654938477	26.966322309205577	35.87048124079517	17.118437795060775
19	19.766487703092398	22.37809127781697	35.40268935575245	22.452731663338184
20	19.579609661901646	26.574833274010356	35.58138295718302	18.26417410690498
21	17.07964696078076	21.64988927561314	36.604608436367045	24.665855327239054
22	17.473224733206447	24.10262093895133	35.14402695749846	23.280127370343767
23	18.42249186351594	21.808803814289945	39.177719567844534	20.59098475434958
24	22.218494702493484	20.460886263995075	35.498941777515384	21.82167725599606
25	17.396197219421847	21.551462862038907	37.75469273183385	23.2976471867054
26	17.475739743341084	19.60160534375713	38.22623581843332	24.696419094468467
27	21.217307532455354	20.390380725644363	38.15185119665461	20.24046054524567
28	20.42290534823299	22.096239633575813	34.463780657014425	23.01707436117677
29	23.491899749138394	19.41229754701257	36.06874076852742	21.027061935321612
30	23.446970585038247	19.207857062169772	36.016096064861685	21.329076287930295
31	24.53878337450419	19.079250526979877	33.34140410031907	23.040561998196864
32	25.373340466299933	18.20215139934738	33.91947278567212	22.50503534868058
33	20.969216702224934	19.10742716594591	37.54769460956599	22.37566152226317
34	21.214920404191968	18.98691981593536	37.250752904517	22.547406875355673
35	24.034502528864003	20.948712975534068	34.56826014582796	20.44852434977397
36	22.03344963479069	22.65529654740261	34.57525102145645	20.73600279635025
37	23.015880797045078	22.432483700389827	36.282175611648334	18.269459890916764
38	20.790608355375195	22.34292376322245	33.315529335035585	23.55093854636677
39	24.411114639703484	20.664218439287016	35.60730034975691	19.31736657125258
40	19.386039136115333	20.73455346847605	37.92034238239663	21.95906501301198
41	20.72023069889574	24.149169940087344	32.386339658512775	22.74425970250414
42	19.92335613182916	19.204745269969287	34.9435508106645	25.92834778753706
43	23.038089615352643	20.36847029836972	34.603853933326654	21.98958615295098
44	21.704452207347664	20.39114801687188	35.6079823864036	22.296417389376852
45	22.708197014810853	20.145060669291087	34.640172384762444	22.506569931135612
46	23.354853010445815	20.114326392899997	33.57150621399326	22.95931438266092
47	20.3156124582519	21.012312892837123	35.53116800907109	23.14090663983989
48	20.82974020797855	19.344392273377338	33.119529053695466	26.706338464948644
49	20.81729303917661	16.79071918633028	37.53115522088396	24.860832553609146
50	19.94249578522666	17.39930901162233	38.61086185987131	24.0473333432797
51	18.642619191232416	16.559252999362723	37.6288569705211	27.16927083888376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	2615.0
2	4776.0
3	75785.0
4	146794.0
5	85109.5
6	23425.0
7	21390.0
8	19355.0
9	19061.5
10	18768.0
11	18395.5
12	18023.0
13	17371.0
14	16719.0
15	15621.0
16	14523.0
17	13517.5
18	12512.0
19	11841.0
20	11170.0
21	10603.5
22	10037.0
23	9314.0
24	8591.0
25	8861.0
26	9768.0
27	10405.0
28	11446.0
29	12487.0
30	14211.0
31	15935.0
32	18478.0
33	21021.0
34	24769.5
35	28518.0
36	32636.5
37	36755.0
38	40214.5
39	43674.0
40	49786.0
41	55898.0
42	66581.5
43	77265.0
44	92333.0
45	107401.0
46	144626.0
47	181851.0
48	228281.5
49	274712.0
50	283978.0
51	293244.0
52	249839.5
53	206435.0
54	176493.0
55	146551.0
56	131471.5
57	116392.0
58	106183.0
59	95974.0
60	87348.0
61	78722.0
62	73225.0
63	67728.0
64	63979.0
65	60230.0
66	46864.0
67	33498.0
68	26044.0
69	18590.0
70	16113.0
71	13636.0
72	14360.5
73	15085.0
74	12781.5
75	7576.5
76	4675.0
77	3570.0
78	2465.0
79	1810.5
80	1156.0
81	804.5
82	453.0
83	360.0
84	267.0
85	169.5
86	72.0
87	53.5
88	35.0
89	27.0
90	19.0
91	11.5
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2345915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.101034434912506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19887176738852	23.387627003041203
2	10.441815777737366	6.49502544132845
3	3.8627887700423753	3.6041017965564373
4	1.903464285731043	2.3679883318458925
5	1.1035710260246923	1.7161100240882836
6	0.7699363811049443	1.4367490740862077
7	0.6049990166667389	1.3171266675308315
8	0.48160454739584224	1.1982719690054833
9	0.40904506034905613	1.1449552056612171
>10	4.755746520917356	31.53491987850701
>50	0.31476655604703246	6.454531084098096
>100	0.14246412007117348	7.912930587782261
>500	0.005947156107869711	1.308675673196642
>1k	0.0038725667679151603	2.5384626011710045
>5k	8.298357359818201E-4	2.012179178164138
>10k+	2.7661191199394E-4	5.570345483936759
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112463	4.793992962234352	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17036	0.7261985195542038	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	9444	0.4025721307037979	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9227	0.393322008683179	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	8146	0.34724190774175534	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	7317	0.3119038839855664	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	6674	0.28449453624705073	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT	5971	0.2545275510834792	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	4684	0.19966622831603023	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT	4153	0.17703113710428553	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4077	0.17379146303254808	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3499	0.14915288917117628	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	3113	0.13269875506998335	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCC	2959	0.1261341523456732	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2638	0.11245079212162418	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2624	0.11185401005577782	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC	2532	0.10793229933735878	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	2372	0.10111193287054304	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2358	0.10051515080469668	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3599874675766172	0.0
2	0.0	0.0	0.0	1.1345253344643775	0.0
3	0.0	0.0	0.0	1.973430409882711	0.0
4	0.0	0.0	0.0	2.5024350839651053	0.0
5	0.0	0.0	0.0	5.236634745930692	0.0
6	0.0	0.0	0.0	6.284413544395258	0.0
7	0.0	0.0	0.0	9.001732799355475	0.0
8	0.0	0.0	0.0	11.730604050018863	0.0
9	0.0	0.0	0.0	14.679261610075386	0.0
10	0.0	0.0	0.0	16.28456273991172	0.0
11	4.262729041759825E-5	0.0	0.0	17.488911576080124	0.0
12	8.52545808351965E-5	0.0	0.0	18.382635346975487	0.0
13	8.52545808351965E-5	0.0	0.0	18.83802269050669	0.0
14	8.52545808351965E-5	0.0	0.0	19.151503784237708	0.0
15	8.52545808351965E-5	0.0	0.0	19.9939469247607	0.0
16	8.52545808351965E-5	0.0	0.0	20.901140919428027	0.0
17	8.52545808351965E-5	0.0	0.0	22.00356790420795	0.0
18	1.2788187125279475E-4	0.0	0.0	22.597920214500526	0.0
19	1.2788187125279475E-4	0.0	0.0	23.266529264700555	0.0
20	1.2788187125279475E-4	0.0	0.0	24.059652630210387	0.0
21	1.2788187125279475E-4	0.0	0.0	24.821402309972868	0.0
22	1.2788187125279475E-4	0.0	0.0	25.61806374058736	0.0
23	1.70509161670393E-4	0.0	0.0	26.272861548692088	0.0
24	2.1313645208799126E-4	0.0	0.0	26.852209052757665	0.0
25	2.1313645208799126E-4	0.0	0.0	27.389611303052327	0.0
26	2.1313645208799126E-4	0.0	0.0	27.946963125262425	0.0
27	2.1313645208799126E-4	0.0	0.0	28.537606861288666	0.0
28	2.1313645208799126E-4	0.0	0.0	29.125437196147345	0.0
29	2.557637425055895E-4	0.0	0.0	29.69792170645569	0.0
30	2.9839103292318773E-4	0.0	0.0	30.227608417184765	0.0
31	2.9839103292318773E-4	0.0	0.0	30.732102399277043	0.0
32	2.9839103292318773E-4	0.0	0.0	31.298746970798174	0.0
33	2.9839103292318773E-4	0.0	0.0	31.839729913487915	0.0
34	2.9839103292318773E-4	0.0	0.0	32.42180556414022	0.0
35	2.9839103292318773E-4	0.0	0.0	32.91351135910721	0.0
36	2.9839103292318773E-4	0.0	0.0	33.43283963826482	0.0
37	2.9839103292318773E-4	0.0	0.0	34.010311541552014	0.0
38	2.9839103292318773E-4	0.0	0.0	34.62107535865537	0.0
39	2.9839103292318773E-4	0.0	0.0	35.260783105952264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAT	20	7.034791E-4	45.0	12
ACAATCG	20	7.034791E-4	45.0	31
CGTTTTT	31635	0.0	44.43101	1
ACTACGG	120	0.0	43.124996	1
CTAGCGG	485	0.0	42.68041	1
ATTACGG	165	0.0	42.272728	1
CACGACG	410	0.0	41.707317	25
TCGGCGT	1445	0.0	41.574398	4
CGCACTT	4350	0.0	41.172413	34
GCGTCCC	1460	0.0	41.147263	7
TACGGGT	225	0.0	41.0	3
TAGGGCG	665	0.0	40.93985	4
GTCGGCG	1475	0.0	40.728813	3
CCGCACT	4435	0.0	40.434048	33
ACCCGCA	4460	0.0	40.358746	31
CGTTTTC	530	0.0	40.33019	1
GCACTTA	4435	0.0	40.28185	35
CCCGCAC	4525	0.0	40.276245	32
GTTTTTT	35210	0.0	40.232887	2
TAGGGCA	1175	0.0	40.21277	4
>>END_MODULE