FastQCFastQC Report
Thu 26 May 2016
SRR1043202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043202_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2534503
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1061714.189026408727865No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC132960.5245998919709308No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA92500.36496307165546854No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT72800.28723580125965525TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT61800.24383478733305897Illumina Single End Adapter 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC48530.1914773823507015Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT42280.1668177153469536TruSeq Adapter, Index 22 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT34270.13521388611495033No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31740.12523165291183322No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30340.11970788750299369No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC30170.11903714456049175TruSeq Adapter, Index 22 (95% over 21bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25830.10191347179308921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT282850.045.203111
CACTTAC36100.042.3684236
CCGCACT36350.041.88720733
GCACTTA36500.041.84109535
CGCACTT36450.041.83539234
CACGACG3750.041.70666525
ATTACGG1600.041.6875041
ACCCGCA36800.041.687531
ACTACGG1050.041.6190451
CCCGCAC37300.041.3753332
ACTTACT36650.041.3560737
CAACGCA41750.040.82155616
TTATGAC40850.040.6511626
TATACGG1700.040.5882341
AACGCAA41850.040.5591417
TCAACGC42550.040.5405415
TATGACC40300.040.5210927
CTTATGA41000.040.4463425
ACCGTAC402.9124203E-740.2500049
ATCAACG42950.040.1629814
Produced by FastQC (version 0.11.5)