##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043202_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2534503
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37762827662859	33.0	31.0	34.0	31.0	34.0
2	32.72130788560913	34.0	31.0	34.0	31.0	34.0
3	32.844929755458956	34.0	31.0	34.0	31.0	34.0
4	36.26979727386395	37.0	37.0	37.0	35.0	37.0
5	36.21809009498114	37.0	37.0	37.0	35.0	37.0
6	36.35647107144872	37.0	37.0	37.0	35.0	37.0
7	36.291906539467504	37.0	37.0	37.0	35.0	37.0
8	36.275406263081955	37.0	37.0	37.0	35.0	37.0
9	37.88111199710555	39.0	38.0	39.0	35.0	39.0
10	37.70438149017776	39.0	37.0	39.0	35.0	39.0
11	37.43860315020341	39.0	37.0	39.0	35.0	39.0
12	37.46354295102432	39.0	37.0	39.0	35.0	39.0
13	37.458008138084665	39.0	37.0	39.0	35.0	39.0
14	38.73724552703232	40.0	38.0	41.0	35.0	41.0
15	38.830362796966504	41.0	38.0	41.0	35.0	41.0
16	38.763290080935	41.0	38.0	41.0	35.0	41.0
17	38.67086564900495	40.0	38.0	41.0	34.0	41.0
18	38.48282799428527	40.0	38.0	41.0	34.0	41.0
19	38.26319203409899	40.0	37.0	41.0	34.0	41.0
20	37.91314904736747	40.0	35.0	41.0	34.0	41.0
21	37.88056435522073	40.0	35.0	41.0	34.0	41.0
22	37.86371016329434	40.0	35.0	41.0	34.0	41.0
23	37.870161132182524	40.0	35.0	41.0	34.0	41.0
24	37.798022334161764	40.0	35.0	41.0	34.0	41.0
25	37.71184251902641	40.0	35.0	41.0	34.0	41.0
26	37.61294383948253	40.0	35.0	41.0	34.0	41.0
27	37.64080531765005	40.0	35.0	41.0	34.0	41.0
28	37.622763910715435	40.0	35.0	41.0	34.0	41.0
29	37.54558230943108	40.0	35.0	41.0	34.0	41.0
30	37.48136498556127	40.0	35.0	41.0	33.0	41.0
31	37.31976920129903	40.0	35.0	41.0	33.0	41.0
32	37.05820470522229	39.0	35.0	41.0	33.0	41.0
33	36.601505699539516	39.0	35.0	41.0	31.0	41.0
34	36.35386819427714	39.0	35.0	41.0	31.0	41.0
35	36.20976065129929	39.0	35.0	41.0	30.0	41.0
36	36.12461614762342	39.0	35.0	41.0	30.0	41.0
37	36.02197472246038	39.0	35.0	41.0	30.0	41.0
38	35.91582176071601	39.0	35.0	41.0	30.0	41.0
39	35.89120549472619	39.0	35.0	41.0	30.0	41.0
40	35.81847723202537	38.0	35.0	41.0	30.0	41.0
41	35.75405947438216	38.0	35.0	41.0	30.0	41.0
42	35.67064824938065	38.0	35.0	41.0	29.0	41.0
43	35.55256395435318	38.0	35.0	41.0	29.0	41.0
44	35.36406230333915	38.0	35.0	40.0	28.0	41.0
45	35.25399259736524	37.0	35.0	40.0	27.0	41.0
46	35.20995122120589	37.0	35.0	40.0	27.0	41.0
47	35.1306406029111	37.0	35.0	40.0	27.0	41.0
48	35.021916722923585	37.0	35.0	40.0	26.0	41.0
49	34.9556769118048	36.0	35.0	40.0	27.0	41.0
50	34.851241051993235	36.0	35.0	40.0	26.0	41.0
51	34.72636686561428	36.0	35.0	40.0	26.0	41.0
52	34.337369496110284	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	7.0
14	25.0
15	63.0
16	116.0
17	220.0
18	475.0
19	922.0
20	1661.0
21	2715.0
22	4355.0
23	7288.0
24	12926.0
25	23862.0
26	36067.0
27	43390.0
28	43071.0
29	41360.0
30	41819.0
31	46512.0
32	55001.0
33	72597.0
34	156754.0
35	228484.0
36	237831.0
37	252921.0
38	366397.0
39	855342.0
40	2315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.47627207385432	17.361825967457918	23.73163495959563	29.43026699909213
2	34.96855201986346	19.922051778987836	23.72757104647341	21.381825154675298
3	25.269017239277286	21.05576517368494	33.879028748437065	19.796188838600703
4	23.162253112345894	23.391055366673466	32.01097019810196	21.43572132287869
5	21.685868984964703	31.433894534747047	28.906692949268553	17.973543531019693
6	78.37808832737622	2.1306346845910222	16.719096406672236	2.7721805813605274
7	78.00914025353293	1.8878257394053193	15.650089978192963	4.452944028868776
8	68.96409276295984	3.5914339024258406	20.762177042205117	6.682296292409203
9	41.375646428510834	15.930499983625982	25.24751400965002	17.446339578213166
10	26.788841836052274	16.374965821701533	35.36507946528373	21.471112876962465
11	23.31897022808811	13.594223403957303	39.66221385415602	23.424592513798565
12	21.796304837674292	14.672186223492337	41.633882461374085	21.89762647745929
13	18.318936690940983	17.34730635552611	42.4083932826278	21.925363670905103
14	16.75188389992042	17.20668707040394	44.23486577052779	21.80656325914785
15	20.300114065755693	16.045512670531462	41.98148512745891	21.67288813625393
16	21.211969368353483	18.285360088348682	38.05045801879106	22.452212524506777
17	20.545803260047432	19.041405750949988	39.483046577573596	20.929744411428985
18	22.451857425301924	23.676200028171206	34.823632088815835	19.048310457711036
19	23.02735486996859	20.49971927435083	33.75912358359805	22.713802272082535
20	21.76071600625448	24.076317921107215	35.06265330914976	19.100312763488542
21	20.700863246167	19.61694265108386	35.0286821518854	24.65351195086374
22	21.875768148627166	21.23453789559531	33.27429480257076	23.615399153206763
23	20.83453836906092	19.438643394779962	36.09559744060276	23.631220795556366
24	23.865033894219103	18.55819464407815	33.83349713928135	23.7432743224214
25	18.95811525967813	19.275061027743902	35.81648946558753	25.950334246990437
26	18.864447980531093	19.56253356180679	36.034954387507135	25.538064070154974
27	21.801789147615924	20.360757118851307	35.37644263983905	22.461011093693713
28	23.421159888151642	21.152904533946103	32.12546996393375	23.3004656139685
29	21.975511569723928	18.79658457693678	35.41396478915196	23.813939064187338
30	26.51154881252853	18.481059205690425	31.290710644256485	23.716681337524555
31	26.44569763776172	19.414141549645038	31.941015654745726	22.199145157847514
32	26.64364571673421	18.07703522150102	31.418862001741566	23.860457060023208
33	24.01780546324072	20.52654899205091	34.50487136925859	20.950774175449784
34	22.936410018058766	22.223962646720086	32.46372168429076	22.37590565093038
35	27.287598397003276	22.746905409068365	29.242932440798057	20.7225637531303
36	25.918020219348726	21.017414459560708	29.333127638830963	23.7314376822596
37	25.43674243036998	23.634692876670496	30.852794413737133	20.075770279222397
38	23.494823245425238	20.094353804276423	30.39956946194185	26.011253488356495
39	24.772588550891435	17.947621289065353	33.924836545863236	23.35495361417998
40	20.81323241676968	17.92331672126646	35.305659531671495	25.957791330292366
41	22.658682984395757	21.7871511692825	30.647586528798744	24.906579317523
42	21.424673792061007	17.877508923840296	32.226909970120374	28.470907313978323
43	23.20119565847821	19.623965724246528	33.51552552906823	23.65931308820704
44	23.210349326870002	20.229252046653723	33.10120366793805	23.459194958538223
45	23.983676484107537	21.223371998376013	32.142554181233955	22.6503973362825
46	23.763436066163663	20.198555693167457	31.92262940702773	24.115378833641152
47	22.553573619758982	20.21035287786205	32.745986096682465	24.4900874056965
48	23.40273418496644	18.779658181505408	30.779249422865153	27.038358210663
49	22.31853740161286	18.400609508057396	34.068217713689826	25.21263537663992
50	22.61646563448534	17.135785595834765	35.33426474539584	24.913484024284053
51	21.35677093299949	16.454310766252792	34.94618076995766	27.242737530790063
52	18.70816487492814	17.38399994002769	37.35541050849022	26.552424676553944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	616.0
1	3760.0
2	6904.0
3	73569.0
4	140234.0
5	80943.0
6	21652.0
7	20204.0
8	18756.0
9	18360.5
10	17965.0
11	17332.5
12	16700.0
13	15929.0
14	14250.0
15	13342.0
16	12710.5
17	12079.0
18	11218.0
19	10357.0
20	9980.5
21	9604.0
22	9022.5
23	8441.0
24	8809.5
25	9178.0
26	10032.5
27	10887.0
28	12078.5
29	13270.0
30	14073.5
31	14877.0
32	17270.0
33	19663.0
34	22701.5
35	25740.0
36	28387.5
37	31035.0
38	34669.0
39	41016.0
40	43729.0
41	51714.0
42	59699.0
43	67495.5
44	75292.0
45	85814.5
46	96337.0
47	117734.5
48	139132.0
49	177706.5
50	216281.0
51	235237.0
52	254193.0
53	240060.5
54	225928.0
55	202986.0
56	180044.0
57	161937.0
58	143830.0
59	134371.5
60	124913.0
61	121039.5
62	117166.0
63	117588.0
64	105585.5
65	93161.0
66	79075.5
67	64990.0
68	52124.5
69	39259.0
70	33508.5
71	27758.0
72	27276.5
73	26795.0
74	21579.0
75	16363.0
76	12574.0
77	8785.0
78	6456.5
79	4128.0
80	3144.5
81	2161.0
82	1541.5
83	922.0
84	693.5
85	465.0
86	342.5
87	220.0
88	139.0
89	57.0
90	56.0
91	48.0
92	40.0
93	31.5
94	23.0
95	12.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2534503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.66110000100362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81832036265389	21.443753618211485
2	10.01864663665099	5.742908662555452
3	3.601513875767073	3.0967004804508655
4	1.8312990045408977	2.0994817560354018
5	1.1449308418476765	1.6407488676214756
6	0.7959910963376884	1.3688388247225798
7	0.6096237991988034	1.2230742070280058
8	0.48646441109698746	1.1154084106704072
9	0.4512560399112251	1.1640145037357255
>10	5.612172004084223	35.88113060706722
>50	0.47201516890977274	8.854278163437746
>100	0.14713063012803237	7.21482610629649
>500	0.00621591947109255	1.2822415645871932
>1k	0.00372955168265553	2.243803967967882
>5k	4.1439463140617E-4	0.8990893519752734
>10k+	2.762630876041133E-4	4.729700907636723
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106171	4.189026408727865	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13296	0.5245998919709308	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9250	0.36496307165546854	No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	7280	0.28723580125965525	TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	6180	0.24383478733305897	Illumina Single End Adapter 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	4853	0.1914773823507015	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	4228	0.1668177153469536	TruSeq Adapter, Index 22 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	3427	0.13521388611495033	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3174	0.12523165291183322	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3034	0.11970788750299369	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	3017	0.11903714456049175	TruSeq Adapter, Index 22 (95% over 21bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2583	0.10191347179308921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25389593147058814	0.0
2	0.0	0.0	0.0	0.8324709025793222	0.0
3	0.0	0.0	0.0	1.4295504878076688	0.0
4	0.0	0.0	0.0	1.823118773187485	0.0
5	0.0	0.0	0.0	3.8711731649163563	0.0
6	0.0	0.0	0.0	4.688177524350928	0.0
7	0.0	0.0	0.0	7.138006938638463	0.0
8	0.0	0.0	0.0	9.854870954976183	0.0
9	0.0	0.0	0.0	12.393830269682065	0.0
10	3.94554672059966E-5	0.0	0.0	13.693730092250828	0.0
11	3.94554672059966E-5	0.0	0.0	14.759303895083178	0.0
12	3.94554672059966E-5	0.0	0.0	15.49814697398267	0.0
13	7.89109344119932E-5	0.0	0.0	15.874275942857436	0.0
14	7.89109344119932E-5	0.0	0.0	16.133458906933626	0.0
15	7.89109344119932E-5	0.0	0.0	16.788695850823615	0.0
16	7.89109344119932E-5	0.0	0.0	17.51684649811028	0.0
17	7.89109344119932E-5	0.0	0.0	18.456794093358738	0.0
18	7.89109344119932E-5	0.0	0.0	18.964585956299914	0.0
19	7.89109344119932E-5	0.0	0.0	19.54047795563864	0.0
20	7.89109344119932E-5	0.0	0.0	20.223531003908853	0.0
21	7.89109344119932E-5	0.0	0.0	20.925128121765884	0.0
22	1.578218688239864E-4	0.0	0.0	21.53988375630252	0.0
23	1.578218688239864E-4	0.0	0.0	22.110686000371672	0.0
24	1.578218688239864E-4	0.0	0.0	22.573340808829187	0.0
25	1.578218688239864E-4	0.0	0.0	23.041164283490687	0.0
26	1.578218688239864E-4	0.0	0.0	23.475411155559886	0.0
27	1.578218688239864E-4	0.0	0.0	23.932502743141356	0.0
28	1.578218688239864E-4	0.0	0.0	24.390422895534154	0.0
29	1.578218688239864E-4	0.0	0.0	24.88657539564956	0.0
30	1.578218688239864E-4	0.0	0.0	25.330922867323494	0.0
31	1.578218688239864E-4	0.0	0.0	25.78986886186365	0.0
32	1.578218688239864E-4	0.0	0.0	26.279590120824476	0.0
33	1.578218688239864E-4	0.0	0.0	26.725042345580178	3.94554672059966E-5
34	1.578218688239864E-4	0.0	0.0	27.23358386239827	3.94554672059966E-5
35	1.578218688239864E-4	0.0	0.0	27.674459252958076	3.94554672059966E-5
36	1.578218688239864E-4	0.0	0.0	28.159406400386978	3.94554672059966E-5
37	1.578218688239864E-4	0.0	0.0	28.71604413172918	3.94554672059966E-5
38	1.578218688239864E-4	0.0	0.0	29.358458048777216	3.94554672059966E-5
39	1.578218688239864E-4	0.0	0.0	30.008171227258362	3.94554672059966E-5
40	1.578218688239864E-4	0.0	0.0	30.634290036350322	3.94554672059966E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28285	0.0	45.20311	1
CACTTAC	3610	0.0	42.36842	36
CCGCACT	3635	0.0	41.887207	33
GCACTTA	3650	0.0	41.841095	35
CGCACTT	3645	0.0	41.835392	34
CACGACG	375	0.0	41.706665	25
ATTACGG	160	0.0	41.687504	1
ACCCGCA	3680	0.0	41.6875	31
ACTACGG	105	0.0	41.619045	1
CCCGCAC	3730	0.0	41.37533	32
ACTTACT	3665	0.0	41.35607	37
CAACGCA	4175	0.0	40.821556	16
TTATGAC	4085	0.0	40.65116	26
TATACGG	170	0.0	40.588234	1
AACGCAA	4185	0.0	40.55914	17
TCAACGC	4255	0.0	40.54054	15
TATGACC	4030	0.0	40.52109	27
CTTATGA	4100	0.0	40.44634	25
ACCGTAC	40	2.9124203E-7	40.250004	9
ATCAACG	4295	0.0	40.16298	14
>>END_MODULE