FastQCFastQC Report
Thu 26 May 2016
SRR1043201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043201_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2465207
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1008944.092719191532394No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC128420.5209298854011043No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG79870.32398901998899077No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC66690.27052494983179914TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT60130.24391460838785547Illumina Single End Adapter 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG45860.1860290028383012Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC41220.16720705401209715TruSeq Adapter, Index 22 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC32560.13207815814250082No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC30850.12514162096732648No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29190.11840790651657243No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT28320.11487879111165918No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC24990.10137079766526706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAA207.034859E-445.00000412
ACTACCG207.034859E-445.00000425
CGTTTTT271650.044.287691
CGAAACG603.6379788E-1241.25000413
ATTACGG1600.040.7812541
TATACGG2100.040.7142831
CACGACG3600.040.62525
CACTTAC31500.040.21428736
ACTTACT31350.040.04784837
ATTGCGG3600.040.01
ACCCGCA32150.039.89113631
GCACTTA32050.039.59438335
GCGTAGG3300.039.5454521
CCCGCAC32600.039.47852732
CGCACTT32350.039.4358634
GCGATCG800.039.3750048
CCGCACT32350.039.29675733
GTTTTTT314200.039.1852342
TACGGGA3850.039.155843
AGGGCGA12700.039.153545
Produced by FastQC (version 0.11.5)