##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043201_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2465207
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.367107102973506	33.0	31.0	34.0	31.0	34.0
2	32.66581832681799	34.0	31.0	34.0	31.0	34.0
3	32.76699035821333	34.0	31.0	34.0	31.0	34.0
4	36.24398519069595	37.0	37.0	37.0	35.0	37.0
5	36.23676267347935	37.0	37.0	37.0	35.0	37.0
6	36.373814450470086	37.0	37.0	37.0	35.0	37.0
7	36.32236075915734	37.0	37.0	37.0	35.0	37.0
8	36.23079076118152	37.0	37.0	37.0	35.0	37.0
9	37.843246023559075	39.0	38.0	39.0	35.0	39.0
10	37.64931180221377	39.0	37.0	39.0	35.0	39.0
11	37.43081656023206	39.0	37.0	39.0	35.0	39.0
12	37.45883084057444	39.0	37.0	39.0	35.0	39.0
13	37.466701579218295	39.0	37.0	39.0	35.0	39.0
14	38.75152390853993	40.0	38.0	41.0	35.0	41.0
15	38.848286979551816	41.0	38.0	41.0	35.0	41.0
16	38.81541225544143	41.0	38.0	41.0	35.0	41.0
17	38.70703717781103	40.0	38.0	41.0	34.0	41.0
18	38.53307977788478	40.0	38.0	41.0	34.0	41.0
19	38.33397844481214	40.0	37.0	41.0	35.0	41.0
20	38.00341391209744	40.0	35.0	41.0	34.0	41.0
21	37.97076432121116	40.0	35.0	41.0	34.0	41.0
22	37.968379937262874	40.0	35.0	41.0	34.0	41.0
23	37.94174485144655	40.0	35.0	41.0	34.0	41.0
24	37.871051396495304	40.0	35.0	41.0	34.0	41.0
25	37.84985642179338	40.0	35.0	41.0	34.0	41.0
26	37.813588473503444	40.0	35.0	41.0	34.0	41.0
27	37.78444122542245	40.0	35.0	41.0	34.0	41.0
28	37.70008279223611	40.0	35.0	41.0	34.0	41.0
29	37.663655830930225	40.0	35.0	41.0	34.0	41.0
30	37.55577645203831	40.0	35.0	41.0	34.0	41.0
31	37.391812127744245	40.0	35.0	41.0	33.0	41.0
32	37.14220915322729	40.0	35.0	41.0	33.0	41.0
33	36.684926661331076	39.0	35.0	41.0	32.0	41.0
34	36.394428540889265	39.0	35.0	41.0	31.0	41.0
35	36.24674682491166	39.0	35.0	41.0	31.0	41.0
36	36.15750117535769	39.0	35.0	41.0	30.0	41.0
37	36.01461702810352	39.0	35.0	41.0	30.0	41.0
38	35.90972441665142	39.0	35.0	41.0	30.0	41.0
39	35.84241972377979	38.0	35.0	41.0	30.0	41.0
40	35.74497192325026	38.0	35.0	41.0	30.0	41.0
41	35.644209593758255	38.0	35.0	41.0	29.0	41.0
42	35.53627017933991	38.0	35.0	40.0	29.0	41.0
43	35.409027314947586	38.0	35.0	40.0	28.0	41.0
44	35.28074356433354	37.0	35.0	40.0	28.0	41.0
45	35.19496172126722	37.0	35.0	40.0	27.0	41.0
46	35.08896615983972	37.0	35.0	40.0	27.0	41.0
47	34.95333008546544	37.0	35.0	40.0	26.0	41.0
48	34.86988313760264	36.0	35.0	40.0	26.0	41.0
49	34.78388549115754	36.0	35.0	40.0	26.0	41.0
50	34.706374758793075	36.0	35.0	40.0	26.0	41.0
51	34.27228058333438	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	11.0
14	17.0
15	49.0
16	102.0
17	221.0
18	433.0
19	768.0
20	1533.0
21	2497.0
22	3854.0
23	6184.0
24	10517.0
25	19718.0
26	32380.0
27	41744.0
28	43512.0
29	41086.0
30	41437.0
31	44968.0
32	53069.0
33	69933.0
34	151488.0
35	224072.0
36	225677.0
37	248261.0
38	378918.0
39	822507.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.66667707823319	17.472569240635778	23.81065768513557	29.050095995995466
2	34.66284981342337	20.025133792010163	23.774068465650146	21.537947928916314
3	25.361561929687852	21.13721079000668	33.61170076184272	19.88952651846275
4	23.38489222203247	23.500095529503202	31.6071226473071	21.507889601157228
5	21.878203331403814	31.56643640878839	28.559346131988107	17.99601412781969
6	78.78056487751333	2.117834323851912	16.332381013034606	2.7692197856001544
7	78.3861558076056	1.879111977209216	15.299242619382468	4.435489595802705
8	69.31377365065083	3.5345104893828387	20.375286943449375	6.77642891651695
9	41.59391077503836	15.969450029956917	24.90350708885704	17.53313210614768
10	27.096020739840508	16.43971479879783	34.91978564071901	21.54447882064265
11	23.53023498635206	13.596383589694497	39.44845199612041	23.42492942783304
12	22.044436836338694	14.631550210590834	41.37149537543906	21.95251757763141
13	18.44056097520411	17.35683859408155	42.19345474842478	22.00914568228956
14	16.864993487362316	17.20078678991257	44.01557354007189	21.91864618265322
15	20.5070405852328	16.060071223227908	41.73592724667746	21.696960944861832
16	21.390942018256478	18.20248766127956	37.88180870815311	22.524761612310854
17	20.692420555352957	19.029436473286015	39.224292321091085	21.053850650269936
18	22.70316448071095	23.66284048357805	34.60390952970683	19.03008550600416
19	23.237196714109608	20.546347629225455	33.43362240980169	22.782833246863245
20	21.925177074379555	24.13566081874666	34.79310256704609	19.14605953982769
21	20.826364682560126	19.73108140614561	34.685728216737985	24.756825694556277
22	21.995191478849442	21.282431860691617	32.98274749341536	23.73962916704358
23	21.037462574136775	19.454187822767015	35.68020859911561	23.828141003980598
24	23.952268511325826	18.546393872806625	33.634011261528954	23.8673263543386
25	18.93305511464149	19.324584101862442	35.6151025045767	26.127258278919374
26	19.00213653457904	19.53649328433677	35.795330777496574	25.66603940358761
27	21.842952741899566	20.34157780665072	35.155141130136336	22.660328321313383
28	23.58442921831716	21.160819355129203	31.93387005634821	23.320881370205424
29	22.07940347402875	18.821989390748932	35.17294896534043	23.92565816988188
30	26.73118322315327	18.46254695853127	30.97342332712831	23.832846491187148
31	26.66056035051012	19.410621501561533	31.594344815668624	22.334473332259723
32	26.879446634704507	17.9965009023583	31.203059215716976	23.920993247220213
33	24.202146107811636	20.482052825584223	34.308031739322495	21.007769327281643
34	23.06370215564048	22.239998507224747	32.22642155405205	22.46987778308272
35	27.44515977765762	22.70275883526211	28.999755395794345	20.852325991285923
36	26.014448279596802	21.013286105385877	29.116297333246255	23.855968281771066
37	25.605395408985938	23.53433200538535	30.68642917207358	20.17384341355513
38	23.701823011211633	19.937230423246405	30.315060763660007	26.04588580188195
39	24.903872169760998	17.80759181683323	33.8560615802243	23.432474433181476
40	21.016044494437995	17.798505358779202	35.14662257571068	26.038827571072126
41	22.816866900020973	21.644064778333018	30.484701690365153	25.05436663128086
42	21.57745779563339	17.82734675019177	31.995933810020823	28.599261644154023
43	23.317676771159583	19.48124437420468	33.45045669592858	23.75062215870716
44	23.376049151247745	20.088779562933254	33.024163893741985	23.511007392077012
45	24.146694374955125	21.02221030526037	32.138355927108755	22.692739392675747
46	23.961273840290083	20.158591144678724	31.828523933284302	24.051611081746888
47	22.798004386649882	20.082694881200645	32.562295985692074	24.557004746457398
48	23.519769333771972	18.638597083328094	30.666268593266206	27.17536498963373
49	22.533645247640465	18.300410472629682	33.90043919232746	25.265505087402396
50	22.84282820874677	16.978939293941643	35.19027002600593	24.987962471305654
51	21.520667432795705	16.44900407957628	34.74762971223106	27.282698775396952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	3586.0
2	6640.0
3	69962.5
4	133285.0
5	77674.0
6	22063.0
7	20076.0
8	18089.0
9	17636.0
10	17183.0
11	16667.0
12	16151.0
13	15151.5
14	14152.0
15	13142.5
16	12133.0
17	11569.5
18	11006.0
19	10311.5
20	9617.0
21	9156.5
22	8696.0
23	8421.5
24	8147.0
25	8548.0
26	9926.0
27	10903.0
28	11767.5
29	12632.0
30	13952.0
31	15272.0
32	17527.0
33	19782.0
34	23477.5
35	27173.0
36	30158.5
37	33144.0
38	36386.0
39	39628.0
40	44846.5
41	50065.0
42	57193.5
43	64322.0
44	72457.0
45	80592.0
46	92516.0
47	104440.0
48	135892.0
49	167344.0
50	202180.5
51	237017.0
52	246721.5
53	256426.0
54	230700.0
55	204974.0
56	181382.0
57	157790.0
58	144559.5
59	131329.0
60	126276.5
61	121224.0
62	117351.5
63	113479.0
64	111987.5
65	110496.0
66	93185.5
67	75875.0
68	61620.0
69	47365.0
70	39132.0
71	30899.0
72	29227.0
73	27555.0
74	23076.0
75	14394.5
76	10192.0
77	7679.0
78	5166.0
79	3929.0
80	2692.0
81	1951.5
82	1211.0
83	863.5
84	516.0
85	383.5
86	251.0
87	161.5
88	72.0
89	72.0
90	72.0
91	55.5
92	39.0
93	30.5
94	22.0
95	14.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2465207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.216368151958804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.97406946885114	20.872795778316796
2	10.190104843064171	5.750554995179142
3	3.7727915594116306	3.1936342680288403
4	1.9259692044153072	2.1737542448447003
5	1.1521266029010109	1.6254414192560285
6	0.8719594132276649	1.476211669031866
7	0.6651049946151991	1.3136793171438326
8	0.5359778901798393	1.2098679576499594
9	0.47085969926128407	1.1957355560049299
>10	5.815612883086982	36.45837619191914
>50	0.46724658755316806	8.635008794526291
>100	0.14729409488108375	7.095931010818885
>500	0.006529655130893002	1.3299899076900805
>1k	0.003627586183829445	2.1660853363607844
>5k	4.353103420595335E-4	0.8462492735514798
>10k+	2.902068947063556E-4	4.656684279677348
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100894	4.092719191532394	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12842	0.5209298854011043	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7987	0.32398901998899077	No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	6669	0.27052494983179914	TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	6013	0.24391460838785547	Illumina Single End Adapter 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	4586	0.1860290028383012	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	4122	0.16720705401209715	TruSeq Adapter, Index 22 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3256	0.13207815814250082	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	3085	0.12514162096732648	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2919	0.11840790651657243	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT	2832	0.11487879111165918	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2499	0.10137079766526706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25275767917258063	0.0
2	0.0	0.0	0.0	0.8215942920817603	0.0
3	0.0	0.0	0.0	1.4209760072886375	0.0
4	0.0	0.0	0.0	1.832057105143706	0.0
5	0.0	0.0	0.0	3.8564712821276266	0.0
6	0.0	0.0	0.0	4.6767269442282124	0.0
7	0.0	0.0	0.0	7.128610295200362	0.0
8	0.0	0.0	0.0	9.893408545408155	0.0
9	0.0	0.0	0.0	12.473110777309978	0.0
10	0.0	0.0	0.0	13.813687856638408	0.0
11	0.0	0.0	0.0	14.906740083084301	0.0
12	0.0	0.0	0.0	15.662782070633419	0.0
13	0.0	0.0	0.0	16.05179605607156	0.0
14	0.0	0.0	0.0	16.319522052306358	0.0
15	0.0	0.0	0.0	16.984942846584484	0.0
16	0.0	0.0	0.0	17.727436276142328	0.0
17	0.0	0.0	0.0	18.6752268673584	0.0
18	0.0	0.0	0.0	19.19510207459252	0.0
19	0.0	0.0	0.0	19.78446434721303	0.0
20	0.0	0.0	0.0	20.484608391911916	0.0
21	0.0	0.0	0.0	21.206981807207264	0.0
22	0.0	0.0	0.0	21.843520645527942	0.0
23	0.0	0.0	0.0	22.416697664739715	0.0
24	0.0	0.0	0.0	22.880593800033832	0.0
25	0.0	0.0	0.0	23.352156634311033	0.0
26	0.0	0.0	0.0	23.796703481695452	0.0
27	0.0	0.0	0.0	24.26214106969516	0.0
28	0.0	0.0	0.0	24.723887284110422	0.0
29	0.0	0.0	0.0	25.224413203434843	0.0
30	0.0	0.0	0.0	25.677113524340957	0.0
31	0.0	0.0	0.0	26.149974424054452	0.0
32	0.0	0.0	0.0	26.640521465337393	0.0
33	0.0	0.0	0.0	27.094763238949103	0.0
34	0.0	0.0	0.0	27.61046029806016	0.0
35	0.0	0.0	0.0	28.06348513532535	0.0
36	0.0	0.0	0.0	28.567053395516076	0.0
37	0.0	0.0	0.0	29.130981698494285	0.0
38	0.0	0.0	0.0	29.77721546304225	0.0
39	0.0	0.0	0.0	30.423124711231146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAA	20	7.034859E-4	45.000004	12
ACTACCG	20	7.034859E-4	45.000004	25
CGTTTTT	27165	0.0	44.28769	1
CGAAACG	60	3.6379788E-12	41.250004	13
ATTACGG	160	0.0	40.781254	1
TATACGG	210	0.0	40.714283	1
CACGACG	360	0.0	40.625	25
CACTTAC	3150	0.0	40.214287	36
ACTTACT	3135	0.0	40.047848	37
ATTGCGG	360	0.0	40.0	1
ACCCGCA	3215	0.0	39.891136	31
GCACTTA	3205	0.0	39.594383	35
GCGTAGG	330	0.0	39.545452	1
CCCGCAC	3260	0.0	39.478527	32
CGCACTT	3235	0.0	39.43586	34
GCGATCG	80	0.0	39.375004	8
CCGCACT	3235	0.0	39.296757	33
GTTTTTT	31420	0.0	39.185234	2
TACGGGA	385	0.0	39.15584	3
AGGGCGA	1270	0.0	39.15354	5
>>END_MODULE