FastQCFastQC Report
Thu 26 May 2016
SRR1043200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043200_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2468002
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1269665.144485296203164No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC132100.5352507818064978No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT90640.36726064241439027No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA88260.3576172142486108No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT75520.3059965105376738Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC59170.23974859015511332No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT51960.21053467541760504No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT45970.18626403058020213No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC43790.17743097452919404No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG34450.13958659676937055No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT34340.13914089210624625No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33650.136345108310285No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31180.12633701269285844No Hit
GGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT30880.12512145452070136No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA253.418304E-546.046
AATACGG650.046.01
CGTTTTT317500.045.3842541
CGGGTAT2200.043.9090925
TCACGAC3350.042.56716524
TATACGG1300.042.461541
ACTACGG1250.042.321
TCATCGG1200.042.1666681
CGACGGT3500.042.0571427
TAGTAGG5200.042.0192341
AGGGTAC6350.042.015755
CTTACGG2250.041.911111
CACTTAC33150.041.6289636
TAGGGTA6800.041.602944
TCCGTAC1550.041.54838646
ACTTACT33000.041.53939437
CACGACG3500.041.39999825
CTTACTG32950.041.25341838
GTTTTTT354500.041.231312
TACGCGG3350.041.194031
Produced by FastQC (version 0.11.5)