##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043200_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2468002
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32223150548501	33.0	31.0	34.0	31.0	34.0
2	32.652882372056425	34.0	31.0	34.0	31.0	34.0
3	32.76156218674053	34.0	31.0	34.0	31.0	34.0
4	36.21523280775299	37.0	37.0	37.0	35.0	37.0
5	36.144396965642656	37.0	37.0	37.0	35.0	37.0
6	36.32028823315378	37.0	37.0	37.0	35.0	37.0
7	36.2605897402028	37.0	37.0	37.0	35.0	37.0
8	36.24347143965037	37.0	37.0	37.0	35.0	37.0
9	37.841052803036625	39.0	38.0	39.0	35.0	39.0
10	37.632787574726436	39.0	37.0	39.0	35.0	39.0
11	37.184222298037035	39.0	35.0	39.0	34.0	39.0
12	37.16771906991972	39.0	35.0	39.0	34.0	39.0
13	37.14900190518484	39.0	35.0	39.0	34.0	39.0
14	38.42076019387343	40.0	37.0	41.0	34.0	41.0
15	38.4647273381464	40.0	37.0	41.0	34.0	41.0
16	38.3017489450981	40.0	37.0	41.0	33.0	41.0
17	38.25132597137279	40.0	37.0	41.0	33.0	41.0
18	38.07407206315068	39.0	36.0	41.0	34.0	41.0
19	37.84408967253673	39.0	36.0	41.0	34.0	41.0
20	37.54567014127217	39.0	35.0	41.0	34.0	41.0
21	37.46886671890866	39.0	35.0	41.0	34.0	41.0
22	37.42961107811096	39.0	35.0	41.0	34.0	41.0
23	37.448760171183004	39.0	35.0	41.0	34.0	41.0
24	37.36813381836806	39.0	35.0	41.0	34.0	41.0
25	37.2625674533489	39.0	35.0	41.0	33.0	41.0
26	37.16971825792686	39.0	35.0	41.0	33.0	41.0
27	37.204538326954356	39.0	35.0	41.0	33.0	41.0
28	37.18021055088286	39.0	35.0	41.0	33.0	41.0
29	37.064572070849216	39.0	35.0	41.0	33.0	41.0
30	36.98737359207975	39.0	35.0	41.0	33.0	41.0
31	36.84855968512181	39.0	35.0	41.0	33.0	41.0
32	36.5660222317486	38.0	35.0	41.0	32.0	41.0
33	36.146634403051536	38.0	35.0	41.0	30.0	41.0
34	35.79954311220169	38.0	35.0	41.0	29.0	41.0
35	35.67909507366688	38.0	35.0	41.0	29.0	41.0
36	35.61613564332606	38.0	35.0	41.0	29.0	41.0
37	35.54003319284182	38.0	35.0	41.0	29.0	41.0
38	35.42791740039109	38.0	35.0	41.0	29.0	41.0
39	35.45091778693858	38.0	35.0	41.0	29.0	41.0
40	35.44501989868728	38.0	35.0	41.0	29.0	41.0
41	35.422155654655064	37.0	35.0	41.0	29.0	41.0
42	35.344763496950165	37.0	35.0	41.0	28.0	41.0
43	35.24141471522309	37.0	35.0	40.0	28.0	41.0
44	35.02294609161581	37.0	35.0	40.0	26.0	41.0
45	34.93781366465667	37.0	35.0	40.0	25.0	41.0
46	34.90140810258663	36.0	35.0	40.0	25.0	41.0
47	34.84448513412874	36.0	35.0	40.0	25.0	41.0
48	34.74552573296132	36.0	35.0	40.0	25.0	41.0
49	34.69247796395627	36.0	35.0	40.0	26.0	41.0
50	34.59763120127131	35.0	35.0	40.0	26.0	41.0
51	34.48403972119958	35.0	35.0	40.0	24.0	41.0
52	34.11331554836666	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	4.0
13	2.0
14	20.0
15	45.0
16	119.0
17	249.0
18	566.0
19	1166.0
20	1911.0
21	3283.0
22	5095.0
23	8214.0
24	14348.0
25	25417.0
26	36896.0
27	44021.0
28	44664.0
29	45464.0
30	47727.0
31	53168.0
32	62655.0
33	80927.0
34	198071.0
35	257283.0
36	233975.0
37	220248.0
38	324521.0
39	756361.0
40	1576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.464523124373482	16.892571399861104	23.199535494703813	31.443369981061604
2	36.35924930368776	18.72757801654942	23.85942150776215	21.05375117200067
3	25.238836921525998	20.429318938963583	35.061073694429744	19.270770445080675
4	22.812987995957865	22.685556980910064	33.44199072772226	21.05946429540981
5	20.99090681450015	30.161928555973617	30.513103311909795	18.334061317616438
6	76.0464942897129	1.8763355945416578	19.03114341074278	3.04602670500267
7	75.96873908530057	1.7802254617297717	17.31915128107676	4.931884171892891
8	66.7818340503776	3.033911641886838	22.85419541799399	7.330058889741581
9	42.36236437409694	13.702136384006172	26.7598243437404	17.175674898156483
10	30.929918209142453	14.90476912093264	35.76281542721602	18.40249724270888
11	27.901395541818847	11.903880142722736	39.15645125084988	21.038273064608536
12	22.592242631894138	12.9996653163166	41.92034690409489	22.48774514769437
13	16.856793470993946	15.742936999240682	44.58225722669592	22.818012303069445
14	18.24779720599902	16.009306313366032	44.48351338451103	21.25938309612391
15	22.904479007715555	13.922841229464158	42.756894038173385	20.415785724646902
16	20.873564932281255	17.990098873501722	40.61159593873911	20.524740255477912
17	21.246943884162167	18.940341215282647	40.22164487711112	19.591070023444065
18	21.350712033458645	26.366226607595944	35.28534417719273	16.997717181752687
19	22.247226704030222	22.776764362427585	33.71930006539703	21.256708868145164
20	21.62721099901864	26.571980087536396	33.797379418655254	18.003429494789714
21	18.486978535673796	21.102008831435306	36.590732098272206	23.82028053461869
22	20.625023804680872	21.368661775800827	34.757467781630645	23.248846637887652
23	22.61635120230859	19.155616567571663	36.389435664962996	21.838596565156752
24	23.96890278046776	17.557076533973635	33.898797488818886	24.575223196739714
25	17.595082986156413	18.496621963839576	37.97079580972787	25.93749924027614
26	17.24577208608421	17.91287041096401	37.48023705005102	27.361120452900767
27	20.988151549309926	21.264326366024015	35.27270237220229	22.474819712463766
28	23.57623697225529	22.26059784392395	31.343775248156202	22.819389935664557
29	23.435475335919502	21.07247076785189	33.89981045396235	21.592243442266255
30	26.43397371639083	18.546743479138186	31.31443977760148	23.704843026869508
31	29.233282631051356	19.025875991996767	29.869708371387055	21.871133005564825
32	25.513188400981846	21.661651813896423	28.652853603846353	24.17230618127538
33	23.98746030189603	21.147875893131367	32.34401754941852	22.520646255554087
34	21.85484452605792	23.596779905364745	32.86277725868942	21.68559830988792
35	24.298278526516594	27.012903555183502	28.214077622303385	20.47474029599652
36	25.213715385968083	24.500304294729098	30.282025703382736	20.003954615920083
37	24.130045275490055	24.284623756382693	31.49721920808816	20.088111760039094
38	21.43178976354152	20.58989417350553	32.545313982727734	25.43300208022522
39	22.518944474113066	17.971379277650502	35.88971970038922	23.619956547847206
40	18.461249221029803	18.41886675942726	37.75296778527732	25.366916234265613
41	19.791718159061457	21.7326404111504	32.010630461401576	26.465010968386572
42	19.372512664090223	17.093543684324406	33.89766296785821	29.63628068372716
43	22.633247460901572	18.553591123508003	33.78506176251073	25.0280996530797
44	21.87186234046812	20.12927866346948	34.29312455986665	23.70573443619576
45	22.763636334168286	20.921376887052766	33.10917090018566	23.20581587859329
46	22.162948004093998	19.812139536353698	33.076472385354634	24.94844007419767
47	20.151482859414212	19.24009786053658	35.028577772627415	25.57984150742179
48	22.178142481245963	17.641963012995937	32.83571083005605	27.344183675702045
49	21.48029053461059	16.639897374475385	35.73177817522028	26.14803391569375
50	20.090056653114544	16.344881406092863	37.87330804432087	25.69175389647172
51	18.022837906938488	15.178107635245027	37.91139553371512	28.887658924101355
52	16.075635270960074	16.13997881687292	40.89692796035011	26.887457951816895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	616.0
1	3613.5
2	6611.0
3	88599.0
4	170587.0
5	96578.5
6	22570.0
7	20094.5
8	17619.0
9	17110.5
10	16602.0
11	16294.0
12	15986.0
13	15364.0
14	13782.0
15	12822.0
16	12017.0
17	11212.0
18	10420.5
19	9629.0
20	9305.0
21	8981.0
22	8667.5
23	8354.0
24	8185.0
25	8016.0
26	8588.0
27	9160.0
28	9812.0
29	10464.0
30	10921.0
31	11378.0
32	14115.0
33	16852.0
34	18659.0
35	20466.0
36	22191.0
37	23916.0
38	25956.5
39	30349.5
40	32702.0
41	40092.5
42	47483.0
43	59668.5
44	71854.0
45	96700.5
46	121547.0
47	159541.0
48	197535.0
49	223570.5
50	249606.0
51	243082.5
52	236559.0
53	209651.5
54	182744.0
55	164834.0
56	146924.0
57	136661.5
58	126399.0
59	124149.0
60	121899.0
61	122168.5
62	122438.0
63	121048.5
64	101227.5
65	82796.0
66	67986.5
67	53177.0
68	42969.5
69	32762.0
70	28769.0
71	24776.0
72	23646.0
73	22516.0
74	18190.5
75	13865.0
76	10918.5
77	7972.0
78	6060.0
79	4148.0
80	3197.0
81	2246.0
82	1704.0
83	1162.0
84	771.5
85	381.0
86	265.5
87	150.0
88	100.5
89	45.5
90	40.0
91	30.5
92	21.0
93	13.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2468002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.61514611379023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41518407866609	22.038165495742927
2	10.155748266549134	6.0152793761744885
3	3.8991266119335983	3.4641961298574415
4	1.9625072381311788	2.324797544265031
5	1.1877993674631857	1.758842591064493
6	0.9013929873214338	1.6016931015282077
7	0.697669274567434	1.4463104253791617
8	0.5871254761578382	1.3910245410834432
9	0.4951985278654599	1.3198839082262477
>10	5.181586455602854	31.728640762190768
>50	0.33382574447883573	6.666211819770281
>100	0.17073960497225701	9.062750454952678
>500	0.006735476684717785	1.3595043113002234
>1k	0.004398678651244267	2.628228450897264
>5k	6.872935392569167E-4	1.4881027697802032
>10k+	2.749174157027667E-4	5.706368317787163
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126966	5.144485296203164	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13210	0.5352507818064978	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	9064	0.36726064241439027	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	8826	0.3576172142486108	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	7552	0.3059965105376738	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	5917	0.23974859015511332	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	5196	0.21053467541760504	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	4597	0.18626403058020213	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	4379	0.17743097452919404	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	3445	0.13958659676937055	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	3434	0.13914089210624625	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3365	0.136345108310285	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3118	0.12633701269285844	No Hit
GGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	3088	0.12512145452070136	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3137355642337405	0.0
2	0.0	0.0	0.0	1.0137755155789987	0.0
3	0.0	0.0	0.0	1.7270245323950304	0.0
4	0.0	0.0	0.0	2.1937988705033464	0.0
5	0.0	0.0	0.0	4.606155100360534	0.0
6	0.0	0.0	0.0	5.685003496755675	0.0
7	0.0	0.0	0.0	8.527383689316297	0.0
8	0.0	0.0	0.0	11.950719650956522	0.0
9	0.0	0.0	0.0	15.307767173608449	0.0
10	0.0	0.0	0.0	17.061533985790934	0.0
11	0.0	0.0	0.0	18.390827884256172	0.0
12	0.0	0.0	0.0	19.307358746062604	0.0
13	0.0	0.0	0.0	19.807196266453595	0.0
14	0.0	0.0	0.0	20.13069681467033	0.0
15	0.0	0.0	0.0	20.82745475895076	0.0
16	0.0	0.0	0.0	21.54905060854894	4.051860573856909E-5
17	0.0	0.0	0.0	22.481707875439323	4.051860573856909E-5
18	0.0	0.0	0.0	23.038717148527432	4.051860573856909E-5
19	0.0	0.0	0.0	23.687744175247833	4.051860573856909E-5
20	0.0	0.0	0.0	24.378951070542083	4.051860573856909E-5
21	0.0	0.0	0.0	25.08154369404887	4.051860573856909E-5
22	8.103721147713819E-5	0.0	0.0	25.74698075609339	4.051860573856909E-5
23	8.103721147713819E-5	0.0	0.0	26.34981657227182	4.051860573856909E-5
24	2.0259302869284548E-4	0.0	0.0	26.863754567459832	4.051860573856909E-5
25	2.0259302869284548E-4	0.0	0.0	27.357149629538387	4.051860573856909E-5
26	2.0259302869284548E-4	0.0	0.0	27.829231904998455	4.051860573856909E-5
27	2.0259302869284548E-4	0.0	0.0	28.32323474616309	4.051860573856909E-5
28	2.0259302869284548E-4	0.0	0.0	28.801678442724114	4.051860573856909E-5
29	2.0259302869284548E-4	0.0	0.0	29.30552730508322	4.051860573856909E-5
30	2.0259302869284548E-4	0.0	0.0	29.77817684102363	4.051860573856909E-5
31	2.0259302869284548E-4	0.0	0.0	30.24454599307456	4.051860573856909E-5
32	2.0259302869284548E-4	0.0	0.0	30.76132029066427	4.051860573856909E-5
33	2.0259302869284548E-4	0.0	0.0	31.273110799748135	4.051860573856909E-5
34	2.0259302869284548E-4	0.0	0.0	31.832713263603512	4.051860573856909E-5
35	2.0259302869284548E-4	0.0	0.0	32.320030534821285	4.051860573856909E-5
36	2.0259302869284548E-4	0.0	0.0	32.8366022393823	4.051860573856909E-5
37	2.0259302869284548E-4	0.0	0.0	33.5666259589741	4.051860573856909E-5
38	2.0259302869284548E-4	0.0	0.0	34.30844059283582	4.051860573856909E-5
39	2.0259302869284548E-4	0.0	0.0	35.13275110798127	4.051860573856909E-5
40	2.0259302869284548E-4	0.0	0.0	35.89223185394501	4.051860573856909E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	25	3.418304E-5	46.0	46
AATACGG	65	0.0	46.0	1
CGTTTTT	31750	0.0	45.384254	1
CGGGTAT	220	0.0	43.909092	5
TCACGAC	335	0.0	42.567165	24
TATACGG	130	0.0	42.46154	1
ACTACGG	125	0.0	42.32	1
TCATCGG	120	0.0	42.166668	1
CGACGGT	350	0.0	42.05714	27
TAGTAGG	520	0.0	42.019234	1
AGGGTAC	635	0.0	42.01575	5
CTTACGG	225	0.0	41.91111	1
CACTTAC	3315	0.0	41.62896	36
TAGGGTA	680	0.0	41.60294	4
TCCGTAC	155	0.0	41.548386	46
ACTTACT	3300	0.0	41.539394	37
CACGACG	350	0.0	41.399998	25
CTTACTG	3295	0.0	41.253418	38
GTTTTTT	35450	0.0	41.23131	2
TACGCGG	335	0.0	41.19403	1
>>END_MODULE