FastQCFastQC Report
Thu 26 May 2016
SRR1043199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043199_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2415014
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1208105.002455472307821No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC124370.5149866626031981No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC85170.35266876299681904No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT73710.30521562193842355No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG72980.30219286513452925No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG57310.23730711291942821No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC49270.20401538044914025No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC45550.1886117430375145No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT42910.17768012939055428No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT32920.13631390956739795No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC32710.13544434939093522No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC32660.13523731125368216No Hit
GGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC30750.12732845441061624No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29220.1209930874106734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT302.1664982E-645.00000425
TCGAACA207.034832E-445.010
CGTTTTT302900.044.4354551
CACGACG2950.042.71186425
TACGCGG2900.042.6724171
AATACGG850.042.352941
TAGGGTA6300.041.7857134
CGCACTT34250.041.64963534
CATATGC9800.041.3265332
CGGTCTA3050.041.31147830
ACTTACT34300.040.99854337
CACTTAC34750.040.9856136
CGACGGT3250.040.8461527
TAGCATA10100.040.76732629
GCACTTA35100.040.7051335
AGGGTAA4700.040.691495
ACCCGCA35850.040.60669731
GTTGATC11250.040.615
CTTACTG34650.040.58441538
CCGCACT35150.040.58321433