##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043199_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2415014
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30949385800662	33.0	31.0	34.0	31.0	34.0
2	32.601322808066534	34.0	31.0	34.0	31.0	34.0
3	32.6819347631111	34.0	31.0	34.0	31.0	34.0
4	36.18646061679146	37.0	37.0	37.0	35.0	37.0
5	36.16165703387227	37.0	37.0	37.0	35.0	37.0
6	36.335542568283245	37.0	37.0	37.0	35.0	37.0
7	36.28752876794917	37.0	37.0	37.0	35.0	37.0
8	36.19756738470253	37.0	37.0	37.0	35.0	37.0
9	37.799442156442986	39.0	38.0	39.0	35.0	39.0
10	37.576960216379696	39.0	37.0	39.0	35.0	39.0
11	37.17914430309721	39.0	35.0	39.0	34.0	39.0
12	37.16877334872593	39.0	35.0	39.0	34.0	39.0
13	37.162214380537755	39.0	35.0	39.0	34.0	39.0
14	38.432976786055896	40.0	37.0	41.0	34.0	41.0
15	38.49024105036244	40.0	37.0	41.0	35.0	41.0
16	38.36987156182117	40.0	37.0	41.0	34.0	41.0
17	38.29480118955832	40.0	37.0	41.0	34.0	41.0
18	38.1342559504831	40.0	37.0	41.0	34.0	41.0
19	37.922104799392464	39.0	36.0	41.0	34.0	41.0
20	37.64452669839595	39.0	35.0	41.0	34.0	41.0
21	37.569081173028394	39.0	35.0	41.0	34.0	41.0
22	37.54538524414351	39.0	35.0	41.0	34.0	41.0
23	37.533574546565774	39.0	35.0	41.0	34.0	41.0
24	37.456259880895104	39.0	35.0	41.0	34.0	41.0
25	37.414678755485475	39.0	35.0	41.0	34.0	41.0
26	37.392696688300774	39.0	35.0	41.0	34.0	41.0
27	37.369486056809606	39.0	35.0	41.0	34.0	41.0
28	37.265847320139756	39.0	35.0	41.0	34.0	41.0
29	37.19705020343567	39.0	35.0	41.0	33.0	41.0
30	37.080099742693	39.0	35.0	41.0	33.0	41.0
31	36.938584621041535	39.0	35.0	41.0	33.0	41.0
32	36.664535278056356	39.0	35.0	41.0	32.0	41.0
33	36.239684324811364	38.0	35.0	41.0	31.0	41.0
34	35.864380910421225	38.0	35.0	41.0	30.0	41.0
35	35.72783014922481	38.0	35.0	41.0	29.0	41.0
36	35.64999167706688	38.0	35.0	41.0	29.0	41.0
37	35.53563788864164	38.0	35.0	41.0	29.0	41.0
38	35.429959826319845	38.0	35.0	40.0	29.0	41.0
39	35.40123908184383	38.0	35.0	40.0	29.0	41.0
40	35.36666578330395	37.0	35.0	40.0	28.0	41.0
41	35.299251681356715	37.0	35.0	40.0	28.0	41.0
42	35.209229014821446	37.0	35.0	40.0	27.0	41.0
43	35.09038125658899	37.0	35.0	40.0	27.0	41.0
44	34.953353479524345	37.0	35.0	40.0	26.0	41.0
45	34.89156004892725	36.0	35.0	40.0	25.0	41.0
46	34.7976065563181	36.0	35.0	40.0	25.0	41.0
47	34.674616379035484	36.0	35.0	40.0	24.0	41.0
48	34.60335964926083	36.0	35.0	40.0	24.0	41.0
49	34.53312113304519	35.0	34.0	40.0	25.0	41.0
50	34.46687845287853	35.0	34.0	40.0	24.0	41.0
51	34.04464901652744	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	13.0
15	48.0
16	108.0
17	237.0
18	466.0
19	926.0
20	1801.0
21	2941.0
22	4603.0
23	7217.0
24	11816.0
25	21066.0
26	33844.0
27	42679.0
28	45061.0
29	45149.0
30	47466.0
31	52664.0
32	59993.0
33	79154.0
34	191197.0
35	255902.0
36	222687.0
37	216624.0
38	337506.0
39	733700.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.787410756210935	16.94892866045497	23.221438881927806	31.042221701406287
2	36.06604350947862	18.7829138878698	24.013939463704972	21.137103138946607
3	25.36622976098689	20.51996385942276	34.739922832745485	19.373883546844866
4	22.971005551106536	22.868563080793734	32.98208623221232	21.17834513588741
5	21.136440616907397	30.23999032717823	30.15750633329662	18.466062722617757
6	76.4520205679967	1.8442129113951304	18.63657933245936	3.0671871881488055
7	76.318895045743	1.7635508531213484	16.938411123082517	4.979142978053129
8	67.04946637990504	3.003460849502322	22.52897912807131	7.418093642521327
9	42.65714401655642	13.651390840798438	26.406016693899083	17.285448448746052
10	31.214684469738064	14.926041836610471	35.40451525332772	18.45475844032374
11	28.157724965569557	11.818109543050268	38.93770388080566	21.086461610574514
12	22.790840964068945	12.957399004726266	41.716735389525695	22.535024641679097
13	16.977541331023343	15.620986048114007	44.48119141338311	22.920281207479544
14	18.346394679285503	15.94392413460129	44.350964425050954	21.358716761062254
15	23.020032182008055	13.948821828776147	42.574038908263056	20.457107080952742
16	21.000457968359605	17.908674649505137	40.476701170262366	20.61416621187289
17	21.374078990846428	18.918772313535243	40.05720049656027	19.64994819905806
18	21.440828086296808	26.48692719793757	35.092674410997205	16.97957030476842
19	22.345667561347472	22.909639447224738	33.453843331757085	21.29084965967071
20	21.64695525574593	26.784151147778022	33.417860103502505	18.15103349297354
21	18.563287831871783	21.2426511813182	36.31109384873131	23.882967138078705
22	20.731142759420855	21.473291666218085	34.37064961114097	23.424915963220087
23	22.84007463310772	19.22485749565013	35.99254497075379	21.942522900488363
24	24.013898056077522	17.547682953390748	33.677113258970756	24.761305731560977
25	17.515674857371426	18.570368536165834	37.854024862795825	26.05993174366691
26	17.323957542275117	17.823664790349042	37.29510470746754	27.557272959908307
27	21.089981258907816	21.261201798416078	35.026546429958586	22.622270512717524
28	23.698537565413698	22.29436351093617	31.07795648389616	22.92914243975397
29	23.547979432003295	21.18849000461281	33.52249717807019	21.741033385313706
30	26.576947380015188	18.46150788359819	30.97559682883826	23.985947907548365
31	29.386123641519262	19.102580771788485	29.473410920185138	22.03788466650711
32	25.67330872616059	21.635236897177407	28.295322511587923	24.396131865074075
33	24.11062627380214	21.11138900229978	32.0285927949072	22.74939192899089
34	21.99142530850753	23.620318557159504	32.593931132490326	21.79432500184264
35	24.449340666348103	26.957856144933324	27.95391662325767	20.638886565460904
36	25.303041721497266	24.524329879661153	30.167651202022018	20.004977196819564
37	24.27224024374186	24.172406453958445	31.401101608520698	20.154251693779003
38	21.547452726982122	20.448701332580267	32.52494602515762	25.478899915279996
39	22.556763646090662	17.805652472408028	35.891551767401765	23.746032114099545
40	18.553929708067944	18.292150687325208	37.69514379626785	25.458775808339002
41	19.868207803350206	21.64844593033415	31.904369912555374	26.578976353760268
42	19.591314998588	16.990957402317335	33.772433617362054	29.645293981732614
43	22.77614125632398	18.4142203730496	33.713634786382194	25.09600358424423
44	21.93424137499824	19.992761946721636	34.232803619357895	23.840193058922228
45	22.831213400833285	20.77963109116552	33.10813105017197	23.28102445782923
46	22.299539464367495	19.747131900684632	32.98974664329068	24.96358199165719
47	20.320544725620636	19.02075929994609	34.92849316815555	25.73020280627773
48	22.339994716386737	17.42548904478401	32.74602134811641	27.48849489071285
49	21.64430516758909	16.435556895322346	35.67167726563904	26.248460671449525
50	20.178392340582704	16.178912420383483	37.87700609603091	25.765689143002895
51	18.15389061926763	15.106247831275512	37.73891165848314	29.000949890973715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	444.0
1	3408.5
2	6373.0
3	84321.0
4	162269.0
5	92508.0
6	22747.0
7	20156.5
8	17566.0
9	16919.5
10	16273.0
11	15796.0
12	15319.0
13	14679.0
14	14039.0
15	12943.5
16	11848.0
17	10992.5
18	10137.0
19	9400.5
20	8664.0
21	8587.0
22	8510.0
23	8033.0
24	7556.0
25	7729.5
26	8070.5
27	8238.0
28	9192.0
29	10146.0
30	11557.5
31	12969.0
32	14646.0
33	16323.0
34	19045.0
35	21767.0
36	23638.5
37	25510.0
38	27104.0
39	28698.0
40	33725.5
41	38753.0
42	46423.5
43	54094.0
44	66330.0
45	78566.0
46	111036.5
47	143507.0
48	189305.5
49	235104.0
50	253295.0
51	271486.0
52	240026.0
53	208566.0
54	184889.5
55	161213.0
56	147030.5
57	132848.0
58	127457.5
59	122067.0
60	121891.5
61	121716.0
62	120026.0
63	118336.0
64	111796.0
65	105256.0
66	85149.5
67	65043.0
68	52038.0
69	39033.0
70	33316.5
71	27600.0
72	25314.5
73	23029.0
74	19630.0
75	12935.0
76	9639.0
77	7129.0
78	4619.0
79	3708.5
80	2798.0
81	2038.5
82	1279.0
83	912.5
84	546.0
85	379.5
86	213.0
87	143.5
88	74.0
89	67.0
90	60.0
91	43.0
92	26.0
93	18.0
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2415014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.197534213177057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68900929055663	21.515373698961486
2	10.386711547373958	6.065327315337047
3	3.896353661933389	3.4129175805281347
4	2.1070233130616285	2.4607954108431427
5	1.275269358760922	1.8617360366719193
6	0.9290457343840341	1.6275506769170427
7	0.7127300259206903	1.456697152160455
8	0.6195818741731833	1.4472210375228707
9	0.5171398609395276	1.3589287904501532
>10	5.337123783303753	31.867924842104188
>50	0.3387821246216555	6.6302188629298655
>100	0.1786343272438885	9.272462651075037
>500	0.006870053305454178	1.3551850504759644
>1k	0.004866287758030043	2.8909416450361327
>5k	5.725044421211815E-4	1.2084210461196294
>10k+	2.8625222106059075E-4	5.568298202866904
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120810	5.002455472307821	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12437	0.5149866626031981	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	8517	0.35266876299681904	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	7371	0.30521562193842355	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7298	0.30219286513452925	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	5731	0.23730711291942821	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	4927	0.20401538044914025	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	4555	0.1886117430375145	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	4291	0.17768012939055428	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	3292	0.13631390956739795	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3271	0.13544434939093522	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC	3266	0.13523731125368216	No Hit
GGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	3075	0.12732845441061624	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2922	0.1209930874106734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3157745669383283	0.0
2	0.0	0.0	0.0	1.0070749072262106	0.0
3	0.0	0.0	0.0	1.7220604104158403	0.0
4	0.0	0.0	0.0	2.1975441964311595	0.0
5	0.0	0.0	0.0	4.635749523605246	0.0
6	0.0	0.0	0.0	5.73238912900712	0.0
7	0.0	0.0	0.0	8.638169385353459	0.0
8	0.0	0.0	0.0	12.171937719615704	0.0
9	0.0	0.0	0.0	15.644505580505951	0.0
10	0.0	0.0	0.0	17.47335626211691	0.0
11	0.0	0.0	0.0	18.82577078228118	0.0
12	0.0	0.0	0.0	19.76452310421389	0.0
13	0.0	0.0	0.0	20.275990118483787	0.0
14	0.0	0.0	0.0	20.6078722525004	0.0
15	0.0	0.0	0.0	21.310725320847002	0.0
16	0.0	0.0	0.0	22.060410415840238	0.0
17	0.0	0.0	0.0	23.011833471772835	0.0
18	0.0	0.0	0.0	23.5929067077872	0.0
19	0.0	0.0	0.0	24.25306851223223	0.0
20	0.0	0.0	0.0	24.972567446813972	0.0
21	0.0	0.0	0.0	25.695875883121175	0.0
22	4.140762745060691E-5	0.0	0.0	26.369826427507252	0.0
23	4.140762745060691E-5	0.0	0.0	26.99267995961928	0.0
24	8.281525490121382E-5	0.0	0.0	27.513008206163608	0.0
25	8.281525490121382E-5	0.0	0.0	28.01172167117872	0.0
26	8.281525490121382E-5	0.0	0.0	28.491180589429295	0.0
27	8.281525490121382E-5	0.0	0.0	28.9896456086797	0.0
28	8.281525490121382E-5	0.0	0.0	29.46984986422439	0.0
29	8.281525490121382E-5	0.0	0.0	29.98019887255312	0.0
30	8.281525490121382E-5	0.0	0.0	30.46110705776447	0.0
31	8.281525490121382E-5	0.0	0.0	30.951331959152203	0.0
32	8.281525490121382E-5	0.0	0.0	31.491163198225767	0.0
33	8.281525490121382E-5	0.0	0.0	32.008385872711294	0.0
34	8.281525490121382E-5	0.0	0.0	32.557906496608304	0.0
35	8.281525490121382E-5	0.0	0.0	33.05902160401555	0.0
36	8.281525490121382E-5	0.0	0.0	33.57156521659916	0.0
37	8.281525490121382E-5	0.0	0.0	34.30323799365138	0.0
38	8.281525490121382E-5	0.0	0.0	35.05727088952693	0.0
39	8.281525490121382E-5	0.0	0.0	35.88215223597047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAT	30	2.1664982E-6	45.000004	25
TCGAACA	20	7.034832E-4	45.0	10
CGTTTTT	30290	0.0	44.435455	1
CACGACG	295	0.0	42.711864	25
TACGCGG	290	0.0	42.672417	1
AATACGG	85	0.0	42.35294	1
TAGGGTA	630	0.0	41.785713	4
CGCACTT	3425	0.0	41.649635	34
CATATGC	980	0.0	41.32653	32
CGGTCTA	305	0.0	41.311478	30
ACTTACT	3430	0.0	40.998543	37
CACTTAC	3475	0.0	40.98561	36
CGACGGT	325	0.0	40.84615	27
TAGCATA	1010	0.0	40.767326	29
GCACTTA	3510	0.0	40.70513	35
AGGGTAA	470	0.0	40.69149	5
ACCCGCA	3585	0.0	40.606697	31
GTTGATC	1125	0.0	40.6	15
CTTACTG	3465	0.0	40.584415	38
CCGCACT	3515	0.0	40.583214	33
>>END_MODULE