FastQCFastQC Report
Thu 26 May 2016
SRR1043198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043198_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences776578
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421565.428430885242693No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT50570.651190221716299No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT47110.6066357790202658Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT40250.5182995140217724No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC21970.2829078341132507No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT16770.21594740000360554No Hit
CGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG14440.18594397471986074No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC14090.1814370224240192No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG13960.17976301157127808No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC13590.17499851914424563No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC11720.1509185168778925No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT11290.14538140405728722No Hit
TCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC9000.11589305903592427No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT8790.11318888765841936No Hit
TGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC8430.1085531652969824No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC8320.1071366945754322No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT8170.10520514359150014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG1050.046.0000041
ACATTGG351.01899786E-746.0000041
GCAAACG351.01899786E-746.0000049
ATTCGTT351.01899786E-746.00000411
CTTGATG351.01899786E-746.00000422
ATCGCGG700.046.0000041
ACGAAGG700.046.0000041
GCATAAT206.310183E-446.09
TCACGAC453.092282E-1046.024
TCGCCGT206.310183E-446.024
CTAGACA253.4161247E-546.040
CTATGAC206.310183E-446.030
ACTATCC301.860617E-646.016
CTGTCGG301.860617E-646.01
GTACCGG600.046.01
CGAACCT206.310183E-446.034
GTCGATG253.4161247E-546.040
AATCAGG301.860617E-646.01
TACTTGG551.8189894E-1246.01
CAACGGC206.310183E-446.02
Produced by FastQC (version 0.11.5)