##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043198_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	776578
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20246259873445	33.0	31.0	34.0	31.0	34.0
2	32.54964343568837	34.0	31.0	34.0	31.0	34.0
3	32.66297397041894	34.0	31.0	34.0	31.0	34.0
4	36.18290371347115	37.0	35.0	37.0	35.0	37.0
5	36.07399771819444	37.0	35.0	37.0	35.0	37.0
6	36.2701595976193	37.0	36.0	37.0	35.0	37.0
7	36.17612912032018	37.0	37.0	37.0	35.0	37.0
8	36.17745802739712	37.0	37.0	37.0	35.0	37.0
9	37.78730404415268	39.0	38.0	39.0	35.0	39.0
10	37.61171704580866	39.0	37.0	39.0	35.0	39.0
11	37.232460873215565	39.0	37.0	39.0	35.0	39.0
12	37.1925151111672	39.0	37.0	39.0	34.0	39.0
13	37.204296284468526	39.0	37.0	39.0	34.0	39.0
14	38.60057199663137	40.0	38.0	41.0	35.0	41.0
15	38.69905663049945	40.0	38.0	41.0	35.0	41.0
16	38.71856272003585	40.0	38.0	41.0	35.0	41.0
17	38.7135883324019	40.0	38.0	41.0	35.0	41.0
18	38.4555820535735	40.0	37.0	41.0	35.0	41.0
19	38.214836114337515	40.0	37.0	41.0	35.0	41.0
20	37.97056702610684	40.0	35.0	41.0	34.0	41.0
21	37.93533811156124	40.0	35.0	41.0	34.0	41.0
22	37.88635397860872	40.0	35.0	41.0	34.0	41.0
23	37.875645459953795	40.0	35.0	41.0	34.0	41.0
24	37.767730994182166	40.0	35.0	41.0	34.0	41.0
25	37.67978103938046	39.0	35.0	41.0	34.0	41.0
26	37.62608392202715	39.0	35.0	41.0	34.0	41.0
27	37.61994931610218	39.0	35.0	41.0	34.0	41.0
28	37.6374697712271	40.0	35.0	41.0	34.0	41.0
29	37.591999773364684	40.0	35.0	41.0	34.0	41.0
30	37.518084468012226	40.0	35.0	41.0	34.0	41.0
31	37.38454990998972	40.0	35.0	41.0	33.0	41.0
32	37.19743670307426	40.0	35.0	41.0	33.0	41.0
33	36.95711828045605	40.0	35.0	41.0	33.0	41.0
34	36.744239471115584	40.0	35.0	41.0	32.0	41.0
35	36.61960807542835	40.0	35.0	41.0	32.0	41.0
36	36.50504649887068	39.0	35.0	41.0	31.0	41.0
37	36.40885783527218	39.0	35.0	41.0	31.0	41.0
38	36.35406746006196	39.0	35.0	41.0	31.0	41.0
39	36.271412530357544	39.0	35.0	41.0	31.0	41.0
40	36.215424593537286	39.0	35.0	41.0	31.0	41.0
41	36.199373404860815	39.0	35.0	41.0	31.0	41.0
42	36.139051067632614	39.0	35.0	41.0	31.0	41.0
43	36.027868417596174	39.0	35.0	41.0	30.0	41.0
44	35.83958855388641	39.0	35.0	41.0	30.0	41.0
45	35.81049682066708	38.0	35.0	41.0	30.0	41.0
46	35.80950657886265	38.0	35.0	41.0	30.0	41.0
47	35.72834924502111	38.0	35.0	41.0	29.0	41.0
48	35.65790171753513	38.0	35.0	40.0	29.0	41.0
49	35.58915009181305	38.0	35.0	40.0	28.0	41.0
50	35.45032308409458	38.0	35.0	40.0	28.0	41.0
51	35.34878016116861	38.0	35.0	40.0	27.0	41.0
52	35.01879399107366	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	4.0
15	6.0
16	15.0
17	38.0
18	114.0
19	254.0
20	466.0
21	798.0
22	1333.0
23	2284.0
24	4203.0
25	7436.0
26	10607.0
27	11207.0
28	10498.0
29	10187.0
30	11150.0
31	13492.0
32	17180.0
33	22433.0
34	43241.0
35	85795.0
36	72853.0
37	59561.0
38	96893.0
39	293640.0
40	887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.41463188501348	19.19704137897288	25.222321518250578	28.166005217763058
2	32.627501680449356	20.17942820940073	26.37674515631398	20.81632495383593
3	21.724153916284006	22.262927870735457	36.96486379990162	19.04805441307892
4	23.644630674574866	24.240320998019516	33.95898415870653	18.15606416869909
5	20.166422432775587	30.634398605162648	29.476755715459362	19.722423246602403
6	74.19589532538909	1.5852882775458483	20.174148636711315	4.044667760353757
7	74.3491317034477	1.2289815060431792	16.7310946228196	7.690792167689531
8	62.21487088225523	3.1152054268856446	25.171586112405965	9.498337578453162
9	35.020049499213215	19.553090610344356	27.40046717779798	18.026392712644448
10	28.1505528098916	18.101851971083395	41.52216519139095	12.225430027634056
11	24.920999564757178	16.62112498680107	37.99296400361587	20.46491144482589
12	17.16453466361396	21.881124626244887	42.393809765406694	18.560530944734463
13	12.579676478087197	25.632196637040966	40.77104939877256	21.01707748609927
14	10.690490845736036	22.55266051832527	46.14925995843303	20.607588677505674
15	11.241240416287868	18.37072386804674	48.21099747868212	22.17703823698328
16	11.918442191254453	24.21006003260458	38.51126351763764	25.360234258503333
17	14.505304039001876	21.699687603820866	41.96951239926962	21.82549595790764
18	15.958345459181178	28.815521428626617	37.90733190999487	17.31880120219733
19	16.631812902245493	23.313949146125694	34.821357287999405	25.23288066362941
20	13.084068825024659	30.971389866825998	34.333705049589355	21.61083625855999
21	14.036709770299957	24.32672571203408	35.17727774930528	26.45928676836068
22	11.460020757734574	27.786004754190824	31.12372485442544	29.630249633649164
23	11.495303755707734	22.624385444861947	43.888057606576545	21.992253192853774
24	16.458230853822798	22.11213812392316	38.81400194185259	22.615629080401455
25	12.738449968966414	27.09141902036885	37.59055754863002	22.579573462034723
26	15.347975348258641	23.92856866921288	35.75506903363216	24.968386948896313
27	18.837386585764726	24.2465019611681	39.47433483822616	17.441776614841007
28	14.294764981753282	23.322834280651783	35.541954575071664	26.840446162523275
29	16.923863411016022	28.713277996543813	32.58848435057393	21.77437424186624
30	21.158853328319886	23.597114520370134	32.999132089757886	22.24490006155209
31	19.778180685005243	25.899008212954783	33.27714666137851	21.045664440661465
32	15.79390608541576	31.196093631290093	32.34562400686087	20.664376276433273
33	17.53461983213534	24.201046128012898	38.03262518381927	20.23170885603249
34	14.327601348480126	28.448397971613925	38.282567881140075	18.941432798765867
35	20.528137547033268	25.34774356214057	33.220874142713285	20.903244748112876
36	23.481994081727787	28.815392658561017	29.766874673245958	17.935738586465234
37	19.433076909209376	26.812374288223463	33.570742410936184	20.183806391630977
38	19.873599303611485	25.896175271511684	28.990391177705266	25.239834247171565
39	25.672244127441157	22.34353793179822	30.834764827229204	21.149453113531415
40	18.503872115872454	20.01846562740639	36.50541220585698	24.972250050864176
41	20.430658607377495	23.24531470116331	31.485568738748714	24.83845795271048
42	20.148652163723412	19.08037569954338	37.738514353999214	23.032457782733996
43	23.619906821980535	23.28768005274422	33.7835993293655	19.30881379590975
44	16.196183770336013	27.361836158119342	34.32713777624398	22.114842295300665
45	19.90540550981357	23.177710416725684	33.519363154763596	23.397520918697158
46	23.462163491626082	21.2806698103732	32.15015104728695	23.107015650713773
47	18.7614122470634	17.88152638884954	36.648475748733546	26.708585615353513
48	20.263128752037787	16.98361272145232	32.0319401270703	30.72131839943959
49	20.93814143588925	17.964583081158622	37.3292830855368	23.767992397415327
50	19.44814300688405	17.255832640121145	37.16677526275532	26.129249090239487
51	16.561504446430362	16.28722420671201	37.544972945409214	29.606298401448406
52	15.693851744448079	16.459776094609943	44.12834254897769	23.718029611964283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	895.0
2	1646.0
3	23809.5
4	45973.0
5	25253.5
6	4534.0
7	4441.0
8	4348.0
9	4660.0
10	4972.0
11	5171.5
12	5371.0
13	5335.0
14	5023.0
15	4747.0
16	4460.0
17	4173.0
18	3984.5
19	3796.0
20	3593.5
21	3391.0
22	3177.0
23	2963.0
24	2908.0
25	2853.0
26	3141.5
27	3430.0
28	3744.0
29	4058.0
30	3978.5
31	3899.0
32	4762.5
33	5626.0
34	7221.0
35	8816.0
36	10436.0
37	12056.0
38	13129.5
39	17381.5
40	20560.0
41	29665.5
42	38771.0
43	58344.5
44	77918.0
45	98918.5
46	119919.0
47	122038.0
48	124157.0
49	107077.0
50	89997.0
51	71428.5
52	52860.0
53	41585.5
54	30311.0
55	24676.0
56	19041.0
57	17097.0
58	15153.0
59	13981.5
60	12810.0
61	10715.0
62	8620.0
63	7128.0
64	4750.5
65	3865.0
66	3338.0
67	2811.0
68	2381.5
69	1952.0
70	1692.0
71	1432.0
72	1405.0
73	1378.0
74	1301.5
75	1225.0
76	1111.5
77	998.0
78	711.5
79	425.0
80	308.5
81	192.0
82	177.0
83	162.0
84	98.5
85	35.0
86	25.0
87	15.0
88	12.5
89	15.0
90	20.0
91	11.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	776578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.430710315666254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3764989064359	22.328989821995524
2	11.804099272697313	7.184142510096373
3	4.6629357158634654	4.256893379700449
4	2.411025241216571	2.9347684271688332
5	1.3243262900957764	2.015009484866278
6	0.9314653287500073	1.7007090952966981
7	0.6374197584628947	1.357799521348641
8	0.44453122223610625	1.0821920680108805
9	0.33894976541168215	0.9283033910525339
>10	3.2250653109371212	21.857294378187582
>50	0.6114261654092229	12.698556234087462
>100	0.21866977436789817	11.169235505892622
>500	0.008492030072539734	1.7345050198524181
>1k	0.004246015036269867	2.651244673893904
>5k	4.2460150362698667E-4	0.6534111952766447
>10k+	4.2460150362698667E-4	5.446945293273133
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42156	5.428430885242693	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	5057	0.651190221716299	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	4711	0.6066357790202658	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	4025	0.5182995140217724	No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2197	0.2829078341132507	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	1677	0.21594740000360554	No Hit
CGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	1444	0.18594397471986074	No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1409	0.1814370224240192	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG	1396	0.17976301157127808	No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1359	0.17499851914424563	No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1172	0.1509185168778925	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT	1129	0.14538140405728722	No Hit
TCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	900	0.11589305903592427	No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	879	0.11318888765841936	No Hit
TGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	843	0.1085531652969824	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	832	0.1071366945754322	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT	817	0.10520514359150014	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6338062628609104	0.0
2	0.0	0.0	0.0	2.1866444838767003	0.0
3	0.0	0.0	0.0	3.6730373510452266	0.0
4	0.0	0.0	0.0	4.751357880341704	0.0
5	0.0	0.0	0.0	9.840608412805926	0.0
6	0.0	0.0	0.0	11.33215208259827	0.0
7	0.0	0.0	0.0	16.211764948273064	0.0
8	0.0	0.0	0.0	21.723123755759243	0.0
9	0.0	0.0	0.0	26.296392635382407	0.0
10	0.0	0.0	0.0	28.636788577580102	0.0
11	0.0	0.0	0.0	30.59113186312257	0.0
12	0.0	0.0	0.0	32.03541691884138	0.0
13	0.0	0.0	0.0	32.634068953794724	0.0
14	0.0	0.0	0.0	33.05231412684881	0.0
15	0.0	0.0	0.0	34.43672110206573	0.0
16	0.0	0.0	0.0	36.00514050101857	0.0
17	0.0	0.0	0.0	37.88801640015556	0.0
18	0.0	0.0	0.0	38.82417477703463	0.0
19	0.0	0.0	0.0	39.81853722356286	0.0
20	0.0	0.0	0.0	40.87406545124894	0.0
21	0.0	0.0	0.0	41.897272392470555	0.0
22	7.726203935728285E-4	0.0	0.0	42.898459652475346	0.0
23	7.726203935728285E-4	0.0	0.0	43.70777951474288	0.0
24	0.001545240787145657	0.0	0.0	44.37017273216599	0.0
25	0.001545240787145657	0.0	0.0	44.96019717272444	0.0
26	0.001545240787145657	0.0	0.0	45.56387124023601	0.0
27	0.001545240787145657	0.0	0.0	46.15299429033529	0.0
28	0.001545240787145657	0.0	0.0	46.7583423686996	0.0
29	0.001545240787145657	0.0	0.0	47.510745861973945	0.0
30	0.001545240787145657	0.0	0.0	48.133992979456025	0.0
31	0.001545240787145657	0.0	0.0	48.67302447403867	0.0
32	0.001545240787145657	0.0	0.0	49.19879265186498	0.0
33	0.001545240787145657	0.0	0.0	49.72790885139677	0.0
34	0.001545240787145657	0.0	0.0	50.3328706195643	0.0
35	0.001545240787145657	0.0	0.0	50.82657505105733	0.0
36	0.001545240787145657	0.0	0.0	51.28911712667627	0.0
37	0.001545240787145657	0.0	0.0	51.79505471440087	0.0
38	0.001545240787145657	0.0	0.0	52.261202351856475	0.0
39	0.001545240787145657	0.0	0.0	52.736492663969365	0.0
40	0.0019315509839320712	0.0	0.0	53.241915171431586	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	105	0.0	46.000004	1
ACATTGG	35	1.01899786E-7	46.000004	1
GCAAACG	35	1.01899786E-7	46.000004	9
ATTCGTT	35	1.01899786E-7	46.000004	11
CTTGATG	35	1.01899786E-7	46.000004	22
ATCGCGG	70	0.0	46.000004	1
ACGAAGG	70	0.0	46.000004	1
GCATAAT	20	6.310183E-4	46.0	9
TCACGAC	45	3.092282E-10	46.0	24
TCGCCGT	20	6.310183E-4	46.0	24
CTAGACA	25	3.4161247E-5	46.0	40
CTATGAC	20	6.310183E-4	46.0	30
ACTATCC	30	1.860617E-6	46.0	16
CTGTCGG	30	1.860617E-6	46.0	1
GTACCGG	60	0.0	46.0	1
CGAACCT	20	6.310183E-4	46.0	34
GTCGATG	25	3.4161247E-5	46.0	40
AATCAGG	30	1.860617E-6	46.0	1
TACTTGG	55	1.8189894E-12	46.0	1
CAACGGC	20	6.310183E-4	46.0	2
>>END_MODULE